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Ldha (NM_017025) Rat Tagged ORF Clone
CAT＃: RR207357
- TrueORF®
Ldha (Myc-DDK-tagged ORF) - Rat lactate dehydrogenase A (Ldha), (10 ug)
"NM_017025" in other vectors (3)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CDK1; Ldh1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>NM_017025 ORF sequence, RR207357 may differ due to SNPs.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCAGCCCTCAAGGACCAGCTGATTGTGAATCTTCTTAAGGAAGAACAGGTCCCCCAGAACAAGATT ACAGTTGTTGGGGTTGGTGCTGTTGGCATGGCTTGTGCCATCAGTATCTTAATGAAGGACTTGGCTGAT GAGCTTGCCCTTGTTGATGTCATAGAAGATAAGCTAAAGGGAGAGATGATGGATCTTCAGCATGGCAGC CTTTTCCTTAAGACACCAAAAATTGTCTCCAGCAAAGATTATAGTGTGACTGCAAACTCCAAGCTGGTC ATTATCACCGCGGGGGCCCGTCAGCAAGAGGGAGAGAGCCGGCTCAATTTGGTCCAGCGAAACGTGAAC ATCTTCAAGTTCATCATTCCAAATGTTGTGAAATACAGTCCACAGTGCAAACTGCTCATCGTCTCAAAC CCAGTGGATATCTTGACCTACGTGGCTTGGAAGATCAGCGGCTTCCCCAAAAACAGAGTTATTGGAAGT GGTTGCAATCTGGATTCGGCTCGGTTCCGTTACCTGATGGGAGAAAGGCTGGGAGTTCATCCACTGAGC TGTCACGGGTGGGTCCTGGGAGAGCATGGCGACTCCAGTGTGCCTGTGTGGAGTGGTGTGAATGTCGCC GGCGTCTCCCTGAAGTCTCTGAACCCGCAGCTGGGCACGGATGCAGACAAGGAGCAGTGGAAGGATGTG CACAAGCAGGTGGTTGACAGTGCATACGAAGTGATCAAGCTGAAAGGTTACACATCCTGGGCCATTGGC CTCTCCGTGGCAGACTTGGCCGAGAGCATAATGAAGAACCTTAGGCGGGTGCATCCCATTTCCACCATG ATTAAGGGTCTCTATGGAATCAAGGAGGATGTCTTCCTCAGCGTCCCATGTATCCTGGGACAAAATGGA ATCTCAGATGTTGTGAAGGTGACACTGACTCCTGACGAGGAGGCCCGCCTGAAGAAGAGTGCAGATACC CTCTGGGGAATCCAGAAGGAGCTGCAGTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC >Peptide sequence encoded by RR207357
Blue=ORF Red=Cloning site Green=Tag(s) MAALKDQLIVNLLKEEQVPQNKITVVGVGAVGMACAISILMKDLADELALVDVIEDKLKGEMMDLQHGS LFLKTPKIVSSKDYSVTANSKLVIITAGARQQEGESRLNLVQRNVNIFKFIIPNVVKYSPQCKLLIVSN PVDILTYVAWKISGFPKNRVIGSGCNLDSARFRYLMGERLGVHPLSCHGWVLGEHGDSSVPVWSGVNVA GVSLKSLNPQLGTDADKEQWKDVHKQVVDSAYEVIKLKGYTSWAIGLSVADLAESIMKNLRRVHPISTM IKGLYGIKEDVFLSVPCILGQNGISDVVKVTLTPDEEARLKKSADTLWGIQKELQF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017025 |
| ORF Size | 996 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq Size | 1598 bp |
| RefSeq ORF | 999 bp |
| Locus ID | 24533 |
| UniProt ID | P04642 |
| MW | 36.5 kDa |
| Gene Summary | mRNA expression in fibroblasts increases in response to induction by epidermal growth factor or serum [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN207357 | Ldha (untagged ORF) - Rat lactate dehydrogenase A (Ldha), (10 ug) |
CNY 2,400.00 |
|
| RR207357L3 | Lenti ORF clone of Ldha (Myc-DDK-tagged ORF) - Rat lactate dehydrogenase A (Ldha), (10 ug) |
CNY 6,080.00 |
|
| RR207357L4 | Lenti ORF clone of Ldha (mGFP-tagged ORF) - Rat lactate dehydrogenase A (Ldha), (10 ug) |
CNY 6,650.00 |
