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Rasa1 (NM_013135) Rat Tagged ORF Clone
CAT＃: RR206131
- TrueORF®
Rasa1 (Myc-DDK-tagged ORF) - Rat RAS p21 protein activator (GTPase activating protein) 1 (Rasa1), (10 ug)
"NM_013135" in other vectors (3)
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CNY 16,720.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GAPX; Rasa |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR206131 representing NM_013135
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGCGGCCGAGGCGGGCAGTGAGGAAGGCGGCCCGGCGACCGCGGGGACCGGCGGCGCCGCGGCCA CGGGCTCCAGCGCCTACCCAGCAGCGTGCCGGGTAAAGTTACCCGCGGCCCCTCCGATGGCAGTCGCCCC GTGTCCTGGGCTGGCGGACACCGACCTGGCTGCGGCCCTCGGTGGGGGAGCCGCGTCGGGGTCCGGGTTC CTGGGGACCGGGCCGGTGTCTGGTGTCCTGGGGGGCGCCGCGCTGACCGGCGGTGCTGCAGCTGGCGTGG CGGGTGCTGCAGCCGCTGGACCTGCCGGAGACATCGCTCTCACCAAGGGGACTCTCTCTCTGCCTGCTGA GACTCTCGGGCCGGGCGGCGGATTCCCTCCTCTGCCGCCTCCTCCTCTGTTGCCTCCTTTGGGCTCCGGT CTGGGGACAGTGGATGAAGGTGACTCCTTGGATGGACCAGAATACGAGGAGGAAGAGGTGGCCATACCGC TGACCGCACCTCCAACTAACCAGTGGTATCATGGAAAACTTGATAGAACTATAGCAGAAGAACGCCTCAG GCAGGCAGGGAAATCTGGAAGCTACCTTATAAGAGAGAGTGATCGGAGGCCAGGGTCCTTTGTACTTTCA TTTCTTAGCCAGACAAATGTTGTCAATCATTTTAGGATTATTGCTATGTGTGGTGATTACTACATTGGTG GAAGGCGTTTTTCTTCACTCTCAGACCTAATAGGATATTACAGTCACGTTTCTTGTTTACTTAAAGGAGA AAAATTACTCTACCCAGTTGCACCACCAGAGCCAGTAGAAGATAGAAGACGTGTACGAGCCATTCTGCCC TACACAAAAGTACCAGACACTGATGAAATAAGTTTCTTAAAGGGAGATATGTTCATTGTTCACAATGAGT TAGAAGATGGATGGATGTGGGTTACAAACCTAAGAACAGATGAGCAAGGCCTTATTGTTGAGGACCTAGT GGAAGAGGTGGGCCGAGAAGAAGATCCACATGAAGGCAAAATATGGTTCCATGGGAAGATTTCCAAACAA GAAGCTTATAATTTATTAATGACAGTTGGCCAAGTCTGCAGCTTTCTTGTGAGGCCCTCAGATAATACTC CTGGAGATTATTCCCTGTATTTTCGGACCAATGAAAATATCCAGCGATTTAAAATTTGTCCAACACCTAA CAATCAGTTCATGATGGGAGGCCGCTATTATAACAGTATTGGGGACATCATAGACCACTATCGGAAAGAA CAGATTGTTGAAGGCTATTATCTTAAGGAACCTGTACCAATGCAGGATCAAGGACAAGTACTCAATGACA CTGTGGATGGGAAGGAAATCTATAATACAATTCGTCGTAAAACAAAGGATGCATTTTATAAAAATATTGT TAAAAAAGGTTATCTTCTGAAGAAGGGCAAAGGAAAACGTTGGAAAAATTTATATTTTATCTTAGAGGGC AGTGATGCCCAACTTATTTATTTTGAAAGTGAAAAACGAGCTACAAAACCAAAAGGGCTAATAGATCTCA GTGTATGTTCTGTCTACGTTGTCCATGATAGTCTCTTCGGCAGGCCAAACTGTTTTCAGATAGTCGTTCA GCACTTTAGTGAAGAACATTACATCTTTTACTTTGCAGGAGAAACTCCAGAACAAGCAGAGGATTGGATG AAAGGCCTGCAAGCATTTTGCAGTTTACGGAAAAGTAGCCCAGGGACATCTAATAAACGCCTTCGTCAGG TCAGCAGCCTTGTATTACATATTGAAGAAGCTCATAAACTACCAGTAAAACACTTCACTAATCCATATTG TAACATCTACTTGAATAGTGTCCAAGTAGCAAAAACTCATGCAAGGGAGGGGCAAAACCCAGTATGGTCA GAGGAGTTTGTCTTTGATGATCTTCCTCCTGACATCAATAGATTTGAAATCACACTTAGTAATAAAACAA AGAAAAGTAAAGATCCAGATATCTTATTTATGCGTTGCCAGTTGAGCAGGTTACAGAAAGGACATGCCAC AGATGAATGGTTTCTGCTAAGTTCTCATATACCACTAAAAGGTATTGAACCAGGATCTCTGCGTGTTCGA GCACGATACTCTATGGAAAAAATCATGCCAGAAGAAGAGTACAGTGAATTTAAAGAGCTTATACTGCAGA AGGAACTTCACGTAGTCTATGCTCTATCACACGTATGTGGACAAGACCGAACACTACTGGCCAGCATCCT ACTGAAAATATTTCTTCATGAAAAGCTTGAGTCATTGTTGTTATGCACACTAAATGACAGAGAGATAAGT ATGGAAGATGAAGCCACTACCCTATTTCGAGCTACAACACTTGCGAGTACCTTGATGGAGCAGTACATGA AAGCCACGGCCACGCAGTTTGTTCATCATGCTTTGAAAGACTCCATTTTAAAGATAATGGAAAGCAAGCA ATCATGTGAGTTAAGTCCATCAAAGTTAGAAAAAAATGAAGATGTGAACACTAATTTAGCACACCTATTG AGCATACTTTCAGAGCTTGTGGAGAAAATATTCATGGCTTCAGAAATACTTCCACCGACACTGAGATATA TTTATGGGTGTTTACAGAAATCAGTTCAGCATAAGTGGCCTACAAATAACACCATGAGAACACGAGTTGT TAGTGGTTTTGTTTTTCTTCGGCTTATCTGCCCAGCTATCCTGAATCCACGGATGTTTAATATCATCTCA GATTCTCCATCTCCTATTGCTGCAAGAACACTGACATTAGTGGCTAAATCTGTGCAGAATTTAGCAAATC TTGTGGAATTTGGAGCTAAGGAGCCTTATATGGAAGGTGTCAATCCATTCATCAAAAGTAATAAACATCG CATGATCATGTTTTTAGATGAACTTGGGAATGTACCTGAGCTTCCGGACACTACAGAACATTCTAGAACT GACCTGTCTCGTGACTTAGCAGCATTGCATGAGATTTGTGTAGCTCATTCAGATGAACTGAGAACACTCA GTAATGAGCGTGGTGTACAGCAGCATGTACTGAAAAAGCTTCTGGCAATAACAGAACTGCTTCAACAAAA GCAAAACCAGTATACAAAAACCAATGATGTCAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR206131 representing NM_013135
Red=Cloning site Green=Tags(s) MMAAEAGSEEGGPATAGTGGAAATGSSAYPAACRVKLPAAPPMAVAPCPGLADTDLAAALGGGAASGSGF LGTGPVSGVLGGAALTGGAAAGVAGAAAAGPAGDIALTKGTLSLPAETLGPGGGFPPLPPPPLLPPLGSG LGTVDEGDSLDGPEYEEEEVAIPLTAPPTNQWYHGKLDRTIAEERLRQAGKSGSYLIRESDRRPGSFVLS FLSQTNVVNHFRIIAMCGDYYIGGRRFSSLSDLIGYYSHVSCLLKGEKLLYPVAPPEPVEDRRRVRAILP YTKVPDTDEISFLKGDMFIVHNELEDGWMWVTNLRTDEQGLIVEDLVEEVGREEDPHEGKIWFHGKISKQ EAYNLLMTVGQVCSFLVRPSDNTPGDYSLYFRTNENIQRFKICPTPNNQFMMGGRYYNSIGDIIDHYRKE QIVEGYYLKEPVPMQDQGQVLNDTVDGKEIYNTIRRKTKDAFYKNIVKKGYLLKKGKGKRWKNLYFILEG SDAQLIYFESEKRATKPKGLIDLSVCSVYVVHDSLFGRPNCFQIVVQHFSEEHYIFYFAGETPEQAEDWM KGLQAFCSLRKSSPGTSNKRLRQVSSLVLHIEEAHKLPVKHFTNPYCNIYLNSVQVAKTHAREGQNPVWS EEFVFDDLPPDINRFEITLSNKTKKSKDPDILFMRCQLSRLQKGHATDEWFLLSSHIPLKGIEPGSLRVR ARYSMEKIMPEEEYSEFKELILQKELHVVYALSHVCGQDRTLLASILLKIFLHEKLESLLLCTLNDREIS MEDEATTLFRATTLASTLMEQYMKATATQFVHHALKDSILKIMESKQSCELSPSKLEKNEDVNTNLAHLL SILSELVEKIFMASEILPPTLRYIYGCLQKSVQHKWPTNNTMRTRVVSGFVFLRLICPAILNPRMFNIIS DSPSPIAARTLTLVAKSVQNLANLVEFGAKEPYMEGVNPFIKSNKHRMIMFLDELGNVPELPDTTEHSRT DLSRDLAALHEICVAHSDELRTLSNERGVQQHVLKKLLAITELLQQKQNQYTKTNDVR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013135 |
| ORF Size | 3114 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013135.1, NP_037267.1 |
| RefSeq Size | 3117 bp |
| RefSeq ORF | 3117 bp |
| Locus ID | 25676 |
| UniProt ID | P50904 |
| MW | 115.4 kDa |
| Gene Summary | may play a role in Ras GTPase mediated signal transduction; may be involved in cell proliferation and cell migration [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN206131 | Rasa1 (untagged ORF) - Rat RAS p21 protein activator (GTPase activating protein) 1 (Rasa1), (10 ug) |
CNY 16,720.00 |
|
| RR206131L3 | Lenti ORF clone of Rasa1 (Myc-DDK-tagged ORF) - Rat RAS p21 protein activator (GTPase activating protein) 1 (Rasa1), (10 ug) |
CNY 18,340.00 |
|
| RR206131L4 | Lenti ORF clone of Rasa1 (mGFP-tagged ORF) - Rat RAS p21 protein activator (GTPase activating protein) 1 (Rasa1), (10 ug) |
CNY 20,050.00 |
