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Mical1 (NM_001106397) Rat Tagged ORF Clone
CAT＃: RR206110
- TrueORF®
Mical1 (Myc-DDK-tagged ORF) - Rat microtubule associated monoxygenase, calponin and LIM domain containing 1 (Mical1), (10 ug)
"NM_001106397" in other vectors (3)
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CNY 16,820.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MICAL-1; Nical |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR206110 representing NM_001106397
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCACCAACCTCCACCAACCCAGCACATGACCACTTTGAGACCTTTGTGCAGGCACAGCTGTGCC AAGATGTACTGAGCAGTTTTCAGGGGCTCTGTAGAGCCCTGGGAGTGGAGTCTGGTGGGGGATTACCCCA GTACCACAAGATCAAGGCCCAGCTCAACTACTGGAGTGCCAAGTCACTGTGGGCCAAGTTGGACAAGAGA GCAAGCCAGCCTGCGTACCAGCAAGGCCAGGCCTGTACCAACACCAAGTGTCTCGTGGTAGGTGCTGGGC CTTGTGGACTTCGGGCTGCAGTGGAGCTTGCACTGTTAGGTGCCCGAGTGGTGCTTGTGGAAAAGCGTAC CAAGTTCTCTAGGCACAATGTACTCCATCTCTGGCCCTTCACCATCCATGATCTCCGGGCACTTGGGGCC AAGAAGTTCTACGGGCGTTTCTGTACTGGGACCCTGGACCATATCAGCATCCGACAGCTCCAGCTGCTTC TGCTGAAGGTGGCGTTACTGTTGGGGGTGGAGATTCACTGGGGCTTCACTTTCACTGGCCTGCAGCCCCC TCCCAAAAAAGGGAGTGGCTGGCGTGCCCGGATCCAGCCCAGTCCCCCAGCCCAACTGGCCAGCTACGAA TTTGATGTCCTCATCTCAGCTGGAGGAGGCAAATTTGTCCCTGAAGGCTTCACCATCAGAGAGATGCGCG GCAAACTGGCCATCGGCATCACAGCCAACTTTGTGAATGGGCGCACGGTGGAGGAGACACAGGTGCCAGA AATCAGTGGCGTAGCTCGGATCTACAACCAAAAATTCTTCCAGAGCCTGCTCAAAGCCACAGGTATTGAT CTGGAGAACATTGTGTACTACAAGGACGACACCCACTACTTTGTCATGACAGCCAAGAAGCAGTGTCTGC TGAGGCTGGGGGTATTGCGCCAGGACTTGCCAGAGACTGATCAGCTGCTGGGCAAAGCTAATGTGGTGCC TGAGGCTCTGCAGCAGTTTGCCAGAGCAGCGGCCGACTTCGCCACCCAAGGCAAGCTTGGGAAACTGGAG TTCGCTCAGGATGCACGTGGGCGGCCCGATGTGGCAGCCTTCGACTTCACAAGCATGATGCGGTCAGAGA GTTCTGCTCGTATCCAAGAAAAGCATGGTGCCCGCCTACTGCTGGGGCTGGTGGGGGACTGCCTTGTGGA GCCCTTCTGGCCTTTGGGCACTGGAGTGGCCCGAGGCTTCTTGGCAGCCTTCGATGCAGCCTGGATGGTG AAGCGGTGGGCGGAGGGTACTGGGCCTCTAGAGCTGTTGGCTGAACGTGAGAGCTTGTACCAGCTTCTGT CACAAACATCCCCAGAGAATATGCATCGAAATGTAGCCCAGTATGGGTTGGACCCTGCCACCCGATACCC CAACCTGAACCTCCGGGCTGTAACGCCCAACCAGGTACAAGACCTCTATGACATAATGGACAAGGAGCAT GCTCGGAAGAAGAGTGACGAGACGGATGCCCGGAAGACAACCACAGGGTCTGCAGGCACTGAGGAGCTTC TGCACTGGTGCCAGGAGCAGACAGCTGGCTTTCCTGGAGTCTCTGTCACTGACTTCTCTTCCTCGTGGGC TGATGGGCGAGCTCTGTGTGCCCTGGTACACCGCCTACAGCCTGGCCTTCTGGAACCCTCAGAGCTGCAG GGCATGAGCGCTCTGGAAGCCACTGCCTGGGCACTGAGGGTGGCAGAATATGAGTTGGGCATCATACCAG TGTTGTCTGCACAGGCCGTAGTGGCAGGCAGTGACCCACTGGGCCTCATTGCCTACCTCAGCCACTTCCA CAGCGCCTTCAAGAACACACCCCACAGCTCAGGCCTTGTCAGCCAGCCTCACGGGACCCCTAGTGCCATA CTTTTCCTTGGCAAACTCCAGAGGAGCCTACAACGGACCCGGACCAAGGTAGAGGAGGAGACTCCGTGTA CTGAGGAGCCTCCTGTCTCTGAGCCCAGTGTGCCCCCAGCTCTGCCCTCCGAACATGAGGAGGCTGGGGC AGAGGATGTGTGTGAGCTCTGTGGGAAACGCCTCTACATCCTGGAACGCTTCTGTGTGGATGGCCATTTC TTCCACCGCGGCTGCTTCTGCTGCCGTACCTGTGAGGCCACATTGCGGCCAGGTGGCTATGGGCAATATC CAGGAGATGGATATTTCTACTGTCTCCAGCACCTACCCCAGGAGGATCAGAAGGAGGCAGACAACAATGG AAGTCCAGAGAACCAGGAGCTCCCAACACCAGGAGACAGTACCACACAGTCAGGCCCCTCCTCTCCTGTA CCTCCTGTGACGGAGGCCAGCCCTGTCCCAAGCCCCAGCCAACCTGCACGTCGGCTGATCCGCCTCTCCA GCGTAGAACGCCTACGGCTATCCTCCCTGAACATCATCCCTGACTCAGGAGTGGAGCCTCCACCCAAGCC CCCACGGAGCTGCTTGGACTTGGCCCAAGAGTCCCTCAAGAGCAGCTTTATGGGCTGGGGTGTGCTCCGG GCACCACAGGTTCCTGAGGCCATAGAGAAGGGGGAGGAGGAAGAAGAAGAGGAGGAGGAAGAAGAAGAGG AGGAGGAGGAGCTTCCGCCACCTTTGGCGCTAGAAGTGGAACAGTCACTGCTGACCTTGGCCAAGAACTC AGGCGACATGACTAAGTACCCAACGTGGCGTCGAACCCTCATGCGCCGTGCCAAAGAGGAGGAGATGAAG AGGTTCTGCAAGGCCCAGGCCATCCAGCGGAGACTAAACGAGATTGAGGCTGCTATGAGGGAACTGGAGA CCGAAGGCATGAAGCTGGAGGTGGCCTTGAGGAAAGAGAGTAGCTCTCCAGAAAAGCAGAAGAAACTCTG GCTAGAGCAGCTGCTACAGCTCATTCAGAAGAAGAACAGCCTAGTGACTGAGGAAGCCGAACTCATGATC ACGGTGCAGGAGCTGGACCTGGAAGAGAAGCAGCGGCAACTGGACCACGAGTTTCGGGGCATCAACCGGG AAGAAACTCTGAAGACCCAGGCCGACCGACTGTCTGAGGACCGGGTCCTAAGGAAGCTGTTGGATGTGGT GAACCAGCGGGATGCTCTGATCCAATTCCAGGAGGAGCGAAGGCTCAGAGAGATGCCCGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR206110 representing NM_001106397
Red=Cloning site Green=Tags(s) MASPTSTNPAHDHFETFVQAQLCQDVLSSFQGLCRALGVESGGGLPQYHKIKAQLNYWSAKSLWAKLDKR ASQPAYQQGQACTNTKCLVVGAGPCGLRAAVELALLGARVVLVEKRTKFSRHNVLHLWPFTIHDLRALGA KKFYGRFCTGTLDHISIRQLQLLLLKVALLLGVEIHWGFTFTGLQPPPKKGSGWRARIQPSPPAQLASYE FDVLISAGGGKFVPEGFTIREMRGKLAIGITANFVNGRTVEETQVPEISGVARIYNQKFFQSLLKATGID LENIVYYKDDTHYFVMTAKKQCLLRLGVLRQDLPETDQLLGKANVVPEALQQFARAAADFATQGKLGKLE FAQDARGRPDVAAFDFTSMMRSESSARIQEKHGARLLLGLVGDCLVEPFWPLGTGVARGFLAAFDAAWMV KRWAEGTGPLELLAERESLYQLLSQTSPENMHRNVAQYGLDPATRYPNLNLRAVTPNQVQDLYDIMDKEH ARKKSDETDARKTTTGSAGTEELLHWCQEQTAGFPGVSVTDFSSSWADGRALCALVHRLQPGLLEPSELQ GMSALEATAWALRVAEYELGIIPVLSAQAVVAGSDPLGLIAYLSHFHSAFKNTPHSSGLVSQPHGTPSAI LFLGKLQRSLQRTRTKVEEETPCTEEPPVSEPSVPPALPSEHEEAGAEDVCELCGKRLYILERFCVDGHF FHRGCFCCRTCEATLRPGGYGQYPGDGYFYCLQHLPQEDQKEADNNGSPENQELPTPGDSTTQSGPSSPV PPVTEASPVPSPSQPARRLIRLSSVERLRLSSLNIIPDSGVEPPPKPPRSCLDLAQESLKSSFMGWGVLR APQVPEAIEKGEEEEEEEEEEEEEEEELPPPLALEVEQSLLTLAKNSGDMTKYPTWRRTLMRRAKEEEMK RFCKAQAIQRRLNEIEAAMRELETEGMKLEVALRKESSSPEKQKKLWLEQLLQLIQKKNSLVTEEAELMI TVQELDLEEKQRQLDHEFRGINREETLKTQADRLSEDRVLRKLLDVVNQRDALIQFQEERRLREMPV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001106397 |
| ORF Size | 3141 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001106397.1, NP_001099867.1 |
| RefSeq Size | 3536 bp |
| RefSeq ORF | 3144 bp |
| Locus ID | 294520 |
| UniProt ID | D3ZBP4 |
| MW | 116.7 kDa |
| Gene Summary | Monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin to form methionine-sulfoxide, resulting in actin filament disassembly and preventing repolymerization. In the absence of actin, it also functions as a NADPH oxidase producing H(2)O(2). Acts as a cytoskeletal regulator that connects NEDD9 to intermediate filaments. Also acts as a negative regulator of apoptosis via its interaction with STK38 and STK38L; acts by antagonizing STK38 and STK38L activation by MST1/STK4. Involved in regulation of lamina-specific connectivity in the nervous system such as the development of lamina-restricted hippocampal connections. Through redox regulation of the actin cytoskeleton controls the intracellular distribution of secretory vesicles containing L1/neurofascin/NgCAM family proteins in neurons, thereby regulating their cell surface levels. May act as Rab effector protein and play a role in vesicle trafficking.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN206110 | Mical1 (untagged ORF) - Rat microtubule associated monoxygenase, calponin and LIM domain containing 1 (Mical1), (10 ug) |
CNY 16,820.00 |
|
| RR206110L3 | Lenti ORF clone of Mical1 (Myc-DDK-tagged ORF) - Rat microtubule associated monoxygenase, calponin and LIM domain containing 1 (Mical1), (10 ug) |
CNY 18,430.00 |
|
| RR206110L4 | Lenti ORF clone of Mical1 (mGFP-tagged ORF) - Rat microtubule associated monoxygenase, calponin and LIM domain containing 1 (Mical1), (10 ug) |
CNY 20,240.00 |
