Hipk1 (NM_001100986) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR205914 representing NM_001100986 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCATCACAGCTGCAGGTGTTTTCCCCTCCATCAGTGTCGTCGAGTGCCTTCTGCAGTGCGAAGAAAC TGAAAATAGAGCCCTCTGGCTGGGATGTTTCAGGACAGAGCAGCAACGACAAATATTATACCCACAGCAA AACCCTCCCAGCTACACAAGGGCAAGCCAGCTCCTCTCACCAGGTAGCAAATTTCAATATTCCTGCTTAC GACCAGGGCCTCCTCCTCCAGGCTCCTGCAGTGGAGCATATTGTGGTAACAGCTGCTGACAGCTCAGGCA GTGCCGCCACGGCAACCTTCCAAAGCAGCCAGACCCTGACTCACAGGAGCAACGTTTCTTTGCTTGAGCC ATATCAAAAATGTGGATTGAAAAGAAAAAGTGAGGAAGTCGACAGCAACGGTAGCGTGCAGATCATAGAA GAACATCCCCCTCTCATGCTGCAAAACAGAACTGTGGTGGGTGCTGCTGCCACGACCACCACTGTGACCA CGAAGAGTAGCAGTTCTAGCGGAGAAGGGGATTACCAGCTGGTCCAGCATGAGATCCTGTGCTCTATGAC CAATAGTTATGAAGTCTTGGAGTTCCTAGGCCGAGGGACATTTGGACAGGTGGCAAAGTGCTGGAAGCGG AGCACCAAGGAAATTGTGGCCATTAAGATCTTGAAGAACCACCCCTCCTATGCCAGACAAGGACAGATCG AAGTGAGCATCCTTTCTCGCCTAAGCAGTGAGAATGCTGATGAGTATAATTTTGTCCGATCTTATGAGTG TTTTCAGCACAAGAATCATACCTGCCTTGTGTTTGAGATGTTGGAGCAGAATTTGTATGATTTTCTAAAG CAGAACAAGTTTAGCCCACTGCCACTCAAGTACATAAGACCAATCTTGCAGCAGGTGGCCACAGCCCTGA TGAAGCTGAAGAGTCTTGGTCTGATTCATGCTGACCTCAAACCTGAGAACATCATGCTGGTTGATCCAGT TCGCCAACCCTACCGAGTGAAGGTCATTGACTTTGGTTCTGCGAGTCATGTTTCCAAAGCCGTATGTTCA ACCTACCTGCAATCACGCTACTACAGAGCTCCTGAAATTATCCTTGGGTTGCCATTCTGTGAGGCTATTG ACATGTGGTCGCTGGGCTGTGTAATAGCTGAGCTGTTCCTGGGCTGGCCTCTTTATCCTGGTGCTTCAGA ATACGATCAGATTCGTTATATCTCACAAACACAAGGCCTGCCAGCTGAATATCTCCTCAGTGCCGGAACA AAAACAACTAGGTTTTTCAACAGAGATCCTAATTTGGGGTACCCACTGTGGAGGCTTAAGACACCAGAAG AACATGAATTAGAGACTGGAATAAAGTCAAAAGAAGCTCGGAAGTACATTTTTAACTGTTTAGATGACAT GGCTCAGGTAAATATGTCTACAGACTTAGAGGGAACAGACATGTTGGCAGAAAAGGCAGACCGGAGAGAA TACATTGATCTCCTAAAGAAAATGCTGACGATTGATGCAGACAAGAGAGTCACGCCTCTGAAAACTCTTA ACCACCAGTTCGTGACAATGACCCACCTTCTGGACTTTCCACACAGTAACCATGTTAAGTCTTGTTTCCA GAACATGGAGATCTGCAAGCGGAGGGTTCACATGTATGACACAGTGAGTCAGATCAAGAGTCCCTTCACT ACCCATGTGGCTCCAAGTACAAGCACAAATCTAACCATGAGCTTCAGCAACCAGCTCAGCACAGTGCACA ACCAGGCCAGTGTTTTAGCCTCCAGCTCTACTGCAGCAGCTGCTACTCTTTCTCTGGCTAACTCAGACGT CTCGCTGCTAAACTACCAGTCAGCTTTGTACCCTCCGTCCGCAGCGCCCGTTCCTGGAGTTGCCCAGCAG GGTGTTTCCTTACAGCCTGGAACCACCCAGATCTGCACCCAGACGGACCCATTCCAGCAAACATTCATAG TGTGTCCACCTGCCTTTCAGACTGGGCTACAAGCAACAACAAAGCATTCTGGATTCCCTGTGAGGATGGA TAATGCTGTGCCACTTGTCCCCCAGGCGCCCGCAGCCCAGCCACTGCAGATACAGTCAGGAGTGCTCACA CAGGGAAGCTGTACACCACTAATGGTAGCAACTCTCCACCCTCAAGTAGCCACCATCACACCGCAGTATG CGGTGCCCTTTACCCTGAGCTGCGCAGCCGGCCGGCCGGCGCTGGTTGAACAGACTGCTGCTGTACTGCA AGCTTGGCCTGGAGGAACCCAACAGATTCTCCTGCCTTCAGCATGGCAGCAGCTGCCTGGGGTCGCTCTA CACAACTCTGTCCAGCCTACTGCAGTGATTCCAGAGGCCATGGGGAGCAGCCAGCAGCTAGCCGACTGGA GGAATGCCCACTCTCATGGCAACCAGTACAGCACTATCATGCAACAGCCATCTTTGCTGACTAACCATGT GACGTTGGCCACTGCGCAGCCTCTGAATGTTGGTGTTGCTCATGTTGTCAGACAACAGCAGTCTAGTTCC CTCCCTTCAAGGAAGAATAAACAGTCTGCTCCAGTCTCATCCACATCTTCTCTGGAAGTTCTGCCTTCCC AAGTTTATTCTCTGGTTGGAAGTAGTCCTCTTCGTACCACATCTTCTTATAATTCCCTAGTCCCTGTCCA AGACCAGCATCAGCCAATCATCATTCCAGACACTCCCAGCCCTCCTGTGAGTGTCATCACTATCCGTAGT GACACCGATGAAGAAGAGGACAACAAATTCAAGCCCAGCAGCTCGAGCCTGAAGGCGAGGTCTAATGTCA TCAGTTATGTGACTGTCAATGATTCTCCAGACTCTGACTCCTCCCTGAGCAGCCCATACCCCACAGACAC TCTGAGTGCTCTGCGGGGCAACAGCGGGACACTTCTGGAGGGACCTGGCAGAACTGCAGCAGATGGCATT GGCACCCGTACGATCATTGTGCCTCCTTTAAAAACACAGCTTGGCGACTGTACTGGAGCAACACAGGCCT CAGGTCTCCTTAGCAGTAGTAAGACCAAGCCAGTGGCCTCAGTGAGTGGGCAGTCATCTGGATGCTGTAT CACTCCCACAGGGTACCGAGCTCAACGAGGGGGAGCCAGCGCGGTGCAGCCACTCAACCTTAGCCAGAAC CAGCAGTCATCGTCAGCTTCAACCTCGCAGGAAAGAAGCAGCAACCCTGCTCCCCGCAGACAGCAGGCAT TTGTGGCCCCGCTCTCCCAAGCCCCCTACGCCTTCCAGCATGGCAGCCCACTGCACTCGACGGGGCACCC ACATTTGGCCCCAGCCCCTGCTCACCTGCCAAGCCAGCCTCACCTGTATACGTACGCTGCCCCCACTTCT GCTGCTGCATTGGGTTCCACCAGTTCCATTGCTCATCTTTTCTCCCCCCAGGGTTCCTCAAGGCATGCTG CAGCTTATACCACCCACCCCAGCACTCTGGTGCATCAGGTTCCTGTCAGTGTTGGGCCTAGCCTCCTCAC CTCTGCCAGTGTGGCCCCTGCTCAGTACCAACACCAGTTTGCCACTCAGTCCTATATTGGGTCTTCCCGA GGCTCAACAATTTACACTGGATACCCGCTGAGTCCTACCAAGATCAGTCAGTATTCTTATTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR205914 representing NM_001100986 Red=Cloning site Green=Tags(s) MASQLQVFSPPSVSSSAFCSAKKLKIEPSGWDVSGQSSNDKYYTHSKTLPATQGQASSSHQVANFNIPAY DQGLLLQAPAVEHIVVTAADSSGSAATATFQSSQTLTHRSNVSLLEPYQKCGLKRKSEEVDSNGSVQIIE EHPPLMLQNRTVVGAAATTTTVTTKSSSSSGEGDYQLVQHEILCSMTNSYEVLEFLGRGTFGQVAKCWKR STKEIVAIKILKNHPSYARQGQIEVSILSRLSSENADEYNFVRSYECFQHKNHTCLVFEMLEQNLYDFLK QNKFSPLPLKYIRPILQQVATALMKLKSLGLIHADLKPENIMLVDPVRQPYRVKVIDFGSASHVSKAVCS TYLQSRYYRAPEIILGLPFCEAIDMWSLGCVIAELFLGWPLYPGASEYDQIRYISQTQGLPAEYLLSAGT KTTRFFNRDPNLGYPLWRLKTPEEHELETGIKSKEARKYIFNCLDDMAQVNMSTDLEGTDMLAEKADRRE YIDLLKKMLTIDADKRVTPLKTLNHQFVTMTHLLDFPHSNHVKSCFQNMEICKRRVHMYDTVSQIKSPFT THVAPSTSTNLTMSFSNQLSTVHNQASVLASSSTAAAATLSLANSDVSLLNYQSALYPPSAAPVPGVAQQ GVSLQPGTTQICTQTDPFQQTFIVCPPAFQTGLQATTKHSGFPVRMDNAVPLVPQAPAAQPLQIQSGVLT QGSCTPLMVATLHPQVATITPQYAVPFTLSCAAGRPALVEQTAAVLQAWPGGTQQILLPSAWQQLPGVAL HNSVQPTAVIPEAMGSSQQLADWRNAHSHGNQYSTIMQQPSLLTNHVTLATAQPLNVGVAHVVRQQQSSS LPSRKNKQSAPVSSTSSLEVLPSQVYSLVGSSPLRTTSSYNSLVPVQDQHQPIIIPDTPSPPVSVITIRS DTDEEEDNKFKPSSSSLKARSNVISYVTVNDSPDSDSSLSSPYPTDTLSALRGNSGTLLEGPGRTAADGI GTRTIIVPPLKTQLGDCTGATQASGLLSSSKTKPVASVSGQSSGCCITPTGYRAQRGGASAVQPLNLSQN QQSSSASTSQERSSNPAPRRQQAFVAPLSQAPYAFQHGSPLHSTGHPHLAPAPAHLPSQPHLYTYAAPTS AAALGSTSSIAHLFSPQGSSRHAAAYTTHPSTLVHQVPVSVGPSLLTSASVAPAQYQHQFATQSYIGSSR GSTIYTGYPLSPTKISQYSYL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001100986
ORF Size	3633 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001100986.1, NP_001094456.1
RefSeq Size	7996 bp
RefSeq ORF	3636 bp
Locus ID	365895
MW	130.8 kDa

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