United States
Germany
Japan
United Kingdom
China
Fyb1 (NM_001109176) Rat Tagged ORF Clone
CAT＃: RR205582
- TrueORF®
Fyb (Myc-DDK-tagged ORF) - Rat FYN binding protein (FYB-120/130) (Fyb), (10 ug)
"NM_001109176" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 10,450.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Fyb; RGD1563421 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR205582 representing NM_001109176
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAAGTTCAACACCGGGAGTAACCCGACAGAGGAGGTCTCCACCAGCAGCAGGCCCTTCAAAGTTG CAGGACAAAACTCCCCTTCTGGAATACAATCAAAAAAGAATTTATTTGACAACCAAGGAAATGCCAGCCC ACCCGCAGGACCCAGCAACATGTCTAAATTTGGGACCACAAAACCACCCTTGGCAGCGAAACCTACCTAT GAGGAGAAGTCTGAGAAAGAGCCTAAACCTCCGTTTTTAAAACCGACTGGAGTGAGCCCAAGGTTTGGAA CACAGCCAAACTCGGTTTCTAGGGATCCTGAGGTCAAAGTGGGGTTTCTGAAACCTGTAAGCCCCAAACC CACCAGCTTGACCAAAGAAGATTCCAAACCTGTGATTTTACGGCCTCCTGGGAATAAGCTTCACAATTTG AACCAAGAGTCTGACTTAAAGACGCTAGGACCGAAGCCAGGGTCTACTCCCCCAGTCCCAGAAAATGATC TGAAGCCAGGGTTCTCAAAGATAGCTGGAGCTAAGAGCAAGTTCATGCCAGCACCGCAAGATGCTGACTC CAAACCCCGATTTCCCAGACACACCTACGGCCAAAAGCCATCCCTAAGTACAGAGGACGCCCAAGAAGAG GAAAGCATCCCTAAGAATACGCCTGTCCAGAAGGGATCCCCAGTGCAGCTCGGAGCCAAGTCCAGGGGCA GCCCTTTCAAACCAGCTAAGGAAGACCCAGAAGATAAAGACCATGGAACACCAAGTTCGCCCTTTGCTGG AGTGGTTCTGAAACCTGCTGCGAGCAGAGGGAGCCCGGGGCTCTCCAAAAACTCTGAAGAGAAAAAAGAA GAGAGAAAAACAGATATTCCAAAGAACATCTTTCTGAACAAACTGAATCAGGAAGAGCCGGCCAGATTCC CTAAGGCCCCTTCCAAGCTGACAGCGGGGACACCATGGGGCCAAAGTCAGGAGAAGGAAGGAGACAAGGA TTCAGCAACCCCGAAGCAGAAGCCCCTGCCTCCCCTGTCCGTCTTGGGCCCGCCTCCATCCAAGCCCAGC AGACCACCCAACGTTGACCTGACCAGATTCCGAAAAGCTGACTCTGCAAACAGTTCCAACAAAAGCCAGA CACCTTACTCGACAACCTCCCTGCCACCACCTCCGCCTACACAGCCAGCCAGCCAGCCACCGCTGCCGGC GTCTCACCCAGCACATCTGCCAGCCCCCAGCCTGCCCCCCAGAAACATTAAGCCTCCCCTTGACCTGAAA CATCCTATAAATGAAGAAAATCAAGATGGTGTCATGCATTCTGATGGTACTGGAAATCTAGAAGAGGAAC AAGAGAGTGATGGAGAAATGTATGAAGACATAGAGTCATCCAAGGAACGAGACAAGAAACGGGAAAAAGA AGAAAAGAAGAGATTAGAGCTAGAACGAAAGGAGCAGAAGGAGAGAGAGAAGAAAGAGCAAGAACTGAGG AAGAAATTCAAGCTTACTGGCCCCATTCAAGTCATCCATCATGCAAAAGCCTGCTGTGATGTCAAAGGAG GAAAGAACGAACTGAGCTTCAAGCAAGGAGAGGACATTGAAATAATCCGCATCACAGACAATCCAGAAGG GAAATGGCTGGGCAGAACTGCAAGGGGATCATATGGTTATATTAAAACGACTGCCGTCAAGATTGACTAT GATTCCCTGAAACGGAAGAAGAATACCATCAATGCTGTTCCACCGAGACCTGTTGAAGAGGACCAAGATG TCTATGATGATGTGGCTGAGCAGGATGCTCCTAACAGCCACAGTCAGAGTGGAAGTGGAGGGATGTTCCC ACCACCGCCAGCAGACGACGACATCTATGATGGGATAGAAGAGGAAGATGCTGATGACGGATCCGTGCCG CAGGTTGATGAGAAGACTAACGCGTGGTCCTGGGGGATTTTGAAGATGTTAAAGGGAAAAGATGAAAGAA AGAAAAGCATTCGAGAGAAACCTAAAGTCTCTGAGTCAGACAGTAATGAAGGTTCATCTTTCCCTTCTCC TCATAAACAACTGGATGTGGGAGAAGAAGTTTACGATGATGTGGATGCCTCAGACTTCCCCCCTCCACCT GCAGAAATGAGTCAAGGTATGAGTGTTGGAAAGACAAAAGCTGAAGAGAAAGATCCTAAGAAGCTAAAAA AGCAGGAAAAGGAAGAAAAAGACCTCAGGAAAAAATTCAAGTACGACGGTGAAATTCGAGTTCTATATTC AACTAAAGTTGCATCCTCCTTAACTTCTAAAAAGTGGGGAACGAGAGATCTGCAGATAAAACCTGGGGAG TCTCTTGAAGTTATACAAAGCACAGACGACACCAAAGTTCTCTGCAGGAATGAAGAGGGCAAATATGGTT ACGTCCTTCGGAGTTACCTGGTGGACAATGATGGAGAAATCTACGACGACATCGCTGATGGTTGCATCTA TGACAACGAC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RR205582 representing NM_001109176
Red=Cloning site Green=Tags(s) MAKFNTGSNPTEEVSTSSRPFKVAGQNSPSGIQSKKNLFDNQGNASPPAGPSNMSKFGTTKPPLAAKPTY EEKSEKEPKPPFLKPTGVSPRFGTQPNSVSRDPEVKVGFLKPVSPKPTSLTKEDSKPVILRPPGNKLHNL NQESDLKTLGPKPGSTPPVPENDLKPGFSKIAGAKSKFMPAPQDADSKPRFPRHTYGQKPSLSTEDAQEE ESIPKNTPVQKGSPVQLGAKSRGSPFKPAKEDPEDKDHGTPSSPFAGVVLKPAASRGSPGLSKNSEEKKE ERKTDIPKNIFLNKLNQEEPARFPKAPSKLTAGTPWGQSQEKEGDKDSATPKQKPLPPLSVLGPPPSKPS RPPNVDLTRFRKADSANSSNKSQTPYSTTSLPPPPPTQPASQPPLPASHPAHLPAPSLPPRNIKPPLDLK HPINEENQDGVMHSDGTGNLEEEQESDGEMYEDIESSKERDKKREKEEKKRLELERKEQKEREKKEQELR KKFKLTGPIQVIHHAKACCDVKGGKNELSFKQGEDIEIIRITDNPEGKWLGRTARGSYGYIKTTAVKIDY DSLKRKKNTINAVPPRPVEEDQDVYDDVAEQDAPNSHSQSGSGGMFPPPPADDDIYDGIEEEDADDGSVP QVDEKTNAWSWGILKMLKGKDERKKSIREKPKVSESDSNEGSSFPSPHKQLDVGEEVYDDVDASDFPPPP AEMSQGMSVGKTKAEEKDPKKLKKQEKEEKDLRKKFKYDGEIRVLYSTKVASSLTSKKWGTRDLQIKPGE SLEVIQSTDDTKVLCRNEEGKYGYVLRSYLVDNDGEIYDDIADGCIYDND SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001109176 |
| ORF Size | 2460 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001109176.1, NP_001102646.1 |
| RefSeq Size | 2707 bp |
| RefSeq ORF | 2463 bp |
| Locus ID | 499537 |
| MW | 90.3 kDa |
| Gene Summary | Acts as an adapter protein of the FYN and LCP2 signaling cascades in T-cells (By similarity). May play a role in linking T-cell signaling to remodeling of the actin cytoskeleton (By similarity). Modulates the expression of IL2 (By similarity). Involved in platelet activation (By similarity). Prevents the degradation of SKAP1 and SKAP2 (By similarity). May be involved in high affinity immunoglobulin epsilon receptor signaling in mast cells (PubMed:12681493).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN205582 | Fyb (untagged ORF) - Rat FYN binding protein (FYB-120/130) (Fyb), (10 ug) |
CNY 10,450.00 |
|
| RR205582L3 | Lenti ORF clone of Fyb (Myc-DDK-tagged ORF) - Rat FYN binding protein (FYB-120/130) (Fyb), (10 ug) |
CNY 12,350.00 |
|
| RR205582L4 | Lenti ORF clone of Fyb (mGFP-tagged ORF) - Rat FYN binding protein (FYB-120/130) (Fyb), (10 ug) |
CNY 13,490.00 |
