Bmi1 (NM_001107368) Rat Tagged ORF Clone
CAT#: RR205336
- TrueORF®
Bmi1 (Myc-DDK-tagged ORF) - Rat Bmi1 polycomb ring finger oncogene (Bmi1), (10 ug)
"NM_001107368" in other vectors (3)
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CNY 3,600.00
Cited in 6 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Pcgf4 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR205336 representing NM_001107368
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCATCGAACAACCAGAATCAAGATCACTGAGCTAAATCCCCACCTAATGTGTGTCCTGTGTGGAGGGT ACTTCATTGATGCCACTACCATAATAGAATGTCTACATTCCTTCTGTAAAACATGTATTGTGCGTTACTT GGAGACCAGCAAGTATTGTCCTATTTGTGATGTCCAAGTTCACAAAACTCGACCACTACTGAATATAAGG TCAGACAAAACTCTTCAAGATATTGTATACAAATTAGTTCCAGGGCTTTTCAAAAATGAGATGAAGAGGA GAAGAGACTTCTATGCAGCTCACCCTTCAGCAGATGCTGCCAATGGCTCCAATGAAGACAGAGGAGAAGT TGCAGATGAGGACCGGCGGGTCATAGCTGACGATGAGATTATAAGCTTGTCTATCGAGTTCTTCCACCCA AGCAGGTCGGATCGGAAAGTAACTAAAGAGAAGCCAAAGGAGGAGGTGAATGATAAAAGGTACTTGCGAT GCCCAGCAGCACTGACGGTGATGCACTTGAGAAAGTTCCTCAGGAGTAAAATGGACATACCTAATACTTT CCAGATTGACGTTATGTATGAAGAGGAGCCTTTAAAGGACTACTACACGCTCATGGACATTGCCTACATT TACACCTGGAGACGGAATGGCCCACTGCCTTTGAAATACCGAGTTCGGCCAACTTGCAAAAGAATGAAGA TGAGTCAGCAGAGAGATGGACTGACCGCGGCTGGAGAGCTGGAGAGTGACTCTGGGAGCGACAAGGCCAA CAGCCCAGCGGGAGGTGCTCCCTCCACCTCTTCCTGCTTGCCCAGCCCCAGCACTCCAGTGCAGTCTCCT CACCCACAGTTCCCTCACATTTCCAGTACCATGAATGGAACCAGCAACAGCCCCAGCGGTAATCACCAAT CTTCCTTTGCCAGTAGACCTCGGAAATCATCAGTAAATGGGTCATCAGCAACTTCGTCTGGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR205336 representing NM_001107368
Red=Cloning site Green=Tags(s) MHRTTRIKITELNPHLMCVLCGGYFIDATTIIECLHSFCKTCIVRYLETSKYCPICDVQVHKTRPLLNIR SDKTLQDIVYKLVPGLFKNEMKRRRDFYAAHPSADAANGSNEDRGEVADEDRRVIADDEIISLSIEFFHP SRSDRKVTKEKPKEEVNDKRYLRCPAALTVMHLRKFLRSKMDIPNTFQIDVMYEEEPLKDYYTLMDIAYI YTWRRNGPLPLKYRVRPTCKRMKMSQQRDGLTAAGELESDSGSDKANSPAGGAPSTSSCLPSPSTPVQSP HPQFPHISSTMNGTSNSPSGNHQSSFASRPRKSSVNGSSATSSG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001107368 |
ORF Size | 972 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001107368.2, NP_001100838.1 |
RefSeq Size | 1222 bp |
RefSeq ORF | 975 bp |
Locus ID | 307151 |
MW | 37 kDa |
Citations (6)
The use of this cDNA Clones has been cited in the following citations: |
---|
Adipocyte-mediated epigenomic instability in human T-ALL cells is cytotoxic and phenocopied by epigenetic-modifying drugs
,null,
Frontiers in Cell and Developmental Biology
,PubMed ID 36060800
[Bmi1]
|
Dkk1 inhibits malignant transformation induced by Bmi1 via the β‐catenin signaling axis in WB‐F344 oval cells
,null,
FEBS Open Bio
,PubMed ID 33639034
[Bmi1]
|
Polycomb Protein BMI1 Regulates Osteogenic Differentiation of Human Adipose Tissue-Derived Mesenchymal Stem Cells Downstream of GSK3.
,null,
Stem cells and development
,PubMed ID 27100571
[Bmi1]
|
CBX8, a component of the repressive Polycomb PRC1 complex, modulates DOT1L-mediated gene expression through AF9/MLLT3
,null,
FEBS letters
,PubMed ID 23891621
[Bmi1]
|
Polycomb Group Proteins as Epigenetic Mediators of Neuroprotection in Ischemic Tolerance
,null,
Science signaling
,PubMed ID 20197544
[Bmi1]
|
Oncogenicity of the developmental transcription factor Sox9
,null,
Cancer research
,PubMed ID 22246670
[Bmi1]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN205336 | Bmi1 (untagged ORF) - Rat Bmi1 polycomb ring finger oncogene (Bmi1), (10 ug) |
CNY 3,600.00 |
|
RR205336L3 | Lenti ORF clone of Bmi1 (Myc-DDK-tagged ORF) - Rat Bmi1 polycomb ring finger oncogene (Bmi1), (10 ug) |
CNY 6,080.00 |
|
RR205336L4 | Lenti ORF clone of Bmi1 (mGFP-tagged ORF) - Rat Bmi1 polycomb ring finger oncogene (Bmi1), (10 ug) |
CNY 6,650.00 |