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Kit (NM_022264) Rat Tagged ORF Clone
CAT＃: RR205111
- TrueORF®
Kit (Myc-DDK-tagged ORF) - Rat v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (Kit), (10 ug)
"NM_022264" in other vectors (3)
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CNY 12,540.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR205111 representing NM_022264
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAGGCGCTCGCGGCGCCTGGGATCTGCTCTGCGTCCTGTTGGTCCTGCTCCGTGGCCAGACAGGGA CTTCTCAGCCATCTGCGAGTCCAGGGGAGCCGTCTCCACCATCCATCCAGCCGGCCCAGTCAGAGTTAAT AGTTGAAGCCGGGGACACCATCAGGCTGACGTGCACTGACCCCGCCTTTGTCAAATGGACTTTCGAGATC CTCGATGTAAGGATTGAGAATAAGCAGAGCGAATGGATTCGAGAAAAAGCCGAGGCCACTCACACGGGCA AATACACGTGCGTCAGCGGCAGCGGCCTCAGGAGCTCTATTTACGTGTTCGTTAGAGATCCTGCCGTACT TTTCCTGGTTGGCCTTCCCTTGTTTGGCAAAGAAGACAACGACGCACTGGTCCGCTGCCCCCTGACAGAC CCACAGGTGTCCAATTACTCCCTCATTGAGTGTGATGGGAAATCTCTCCCCACGGACCTGAAGTTCGTCC CCAACCCCAAGGCTGGCATCACCATCAAAAACGTGAAGCGCGCCTACCACCGGCTGTGCATCCGGTGTGC TGCCCAGCGTGAGGGCAAATGGATGCGGTCTGACAAATTCACCCTCAAAGTGAGAGCAGCCATCAAAGCT ATCCCAGTGGTGTCTGTGCCCGAAACAAGTCATCTCCTTAAGGAAGGGGACACATTTACGGTGATATGCA CCATAAAAGACGTGTCTACATCCGTGGACTCCATGTGGATAAAGTTGAACCCTCAGCCTCAGAGCAAAGC CCAGGTAAAGCGCAATAGCTGGCATCAGGGCGACTTCAATTACGAACGCCAGGAGACGCTGACTATCAGC TCAGCAAGAGTTAACGATTCCGGAGTGTTCATGTGTTATGCCAATAATACTTTTGGATCAGCAAATGTCA CAACAACCTTGAAAGTAGTAGAAAAGGGATTCATCAACATCTTCCCTGTGAAGAACACTACGGTATTTGT AACTGATGGGGAAAATGTAGACTTGGTTGTTGAGTTCGAGGCCTACCCTAAACCTGAACACCAGCAGTGG ATCTACATGAACAGGACGCCTACTAACAGAGGGGAGGATTATGTCAAATCCGACAACCAAAGCAACATCA GATATGTGAACGAACTTCGCCTGACCAGATTGAAAGGCACAGAAGGAGGCACTTACACCTTTCTGGTGTC CAACTCTGATGTCAGTGCTTCCGTGACATTTGATGTTTATGTGAACACAAAACCAGAAATCCTGACATAT GACAGGCTCATGAATGGCAGGCTCCAGTGTGTGGCGGCGGGATTCCCGGAGCCCACAATAGATTGGTATT TTTGTACAGGGGCAGAGCAAAGGTGTACCGTTCCTGTCCCGCCAGTAGACGTACAGATCCAGAATGCGTC TGTGTCACCATTTGGAAAACTGGTGGTTCAGAGTTCCATAGACTCCAGCGTCTTCCGGCACAACGGCACG GTGGAGTGTAAGGCCTCCAACGCTGTGGGCAAGAGCTCTGCCTTCTTTAACTTTGCATTTAAAGGTAACA GCAAAGAGCAAATCCAGCCCCACACCCTGTTCACGCCGCTGCTCATTGGCTTCGTGGTCACAGCCGGCTT GATGGGGATCATTGTGATGGTTCTTGCCTACAAATATTTGCAGAAACCCATGTATGAAGTACAATGGAAG GTTGTCGAGGAGATAAATGGGAACAATTATGTTTACATAGACCCAACGCAGCTTCCTTATGACCACAAAT GGGAGTTTCCCAGAAACAGGCTGAGTTTTGGAAAGACCTTGGGAGCTGGTGCCTTTGGGAAGGTAGTTGA GGCCACTGCCTATGGCTTAATTAAGTCGGATGCCGCCATGACGGTTGCCGTGAAGATGCTCAAACCAAGT GCCCATTTAACGGAAAGGGAGGCCCTAATGTCAGAACTGAAGGTCCTGAGCTACCTGGGTAATCACATGA ATATCGTCAACCTCCTTGGAGCGTGTACCGTGGGAGGGCCCACCCTGGTCATTACAGAATACTGTTGCTA TGGTGATCTTTTGAATTTCTTGAGAAGAAAGCGTGACTCGTTTATTTTCTCAAAGCAAGAAGAACAGGCA GACGCCGCACTTTATAAGAACCTTCTGCATTCAAAGGAGTCTTCCTGTGACAGCTCAAACGAGTACATGG ACATGAAGCCTGGCGTTTCCTACGTCGTACCAACCAAGACAGACAAAAGGAGATCCGCAAGAATAGACTC GTATATAGAAAGAGACGTGACTCCCGCCATCATGGAAGATGACGAGCTGGCTCTGGACCTGGAAGATTTG CTGAGCTTTTCCTACCAGGTGGCCAAGGGCATGGCGTTCCTCGCCTCCAAGAACTGTATTCACAGAGATT TGGCAGCCAGGAATATCCTCCTCACTCACGGGCGGATCACAAAGATTTGCGATTTCGGCCTAGCCAGAGA CATCAGGAATGATTCGAATTACGTGGTAAAAGGAAATGCACGGCTGCCCGTGAAGTGGATGGCACCGGAG AGCATTTTCAACTGCGTGTACACATTTGAAAGTGACGTCTGGTCCTATGGGATTTTCCTCTGGGAGCTAT TCTCTCTAGGAAGCAGCCCCTACCCAGGGATGCCGGTCGATTCCAAGTTTTACAAGATGATCAAGGAAGG TTTCCGAATGCTCAGCCCTGAGCACGCGCCTGCCGCAATGTATGAAGTTATGAAGACTTGCTGGGATGCT GATCCCCTGAAAAGGCCAACATTCAAGCAGGTTGTTCAGCTCATTGAGAAGCAGATCTCAGACAGCAGCA AACATATTTACTCCAACTTAGCAAACTGTAACCCCAACCCAGAGAACCCCGTGGTGGTGGACCATTCTGT GAGGGTCAATTCCGTCGGCAGCAGCACCTCTTCCACACAGCCTCTCCTCGTGCATGAGGACGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR205111 representing NM_022264
Red=Cloning site Green=Tags(s) MRGARGAWDLLCVLLVLLRGQTGTSQPSASPGEPSPPSIQPAQSELIVEAGDTIRLTCTDPAFVKWTFEI LDVRIENKQSEWIREKAEATHTGKYTCVSGSGLRSSIYVFVRDPAVLFLVGLPLFGKEDNDALVRCPLTD PQVSNYSLIECDGKSLPTDLKFVPNPKAGITIKNVKRAYHRLCIRCAAQREGKWMRSDKFTLKVRAAIKA IPVVSVPETSHLLKEGDTFTVICTIKDVSTSVDSMWIKLNPQPQSKAQVKRNSWHQGDFNYERQETLTIS SARVNDSGVFMCYANNTFGSANVTTTLKVVEKGFINIFPVKNTTVFVTDGENVDLVVEFEAYPKPEHQQW IYMNRTPTNRGEDYVKSDNQSNIRYVNELRLTRLKGTEGGTYTFLVSNSDVSASVTFDVYVNTKPEILTY DRLMNGRLQCVAAGFPEPTIDWYFCTGAEQRCTVPVPPVDVQIQNASVSPFGKLVVQSSIDSSVFRHNGT VECKASNAVGKSSAFFNFAFKGNSKEQIQPHTLFTPLLIGFVVTAGLMGIIVMVLAYKYLQKPMYEVQWK VVEEINGNNYVYIDPTQLPYDHKWEFPRNRLSFGKTLGAGAFGKVVEATAYGLIKSDAAMTVAVKMLKPS AHLTEREALMSELKVLSYLGNHMNIVNLLGACTVGGPTLVITEYCCYGDLLNFLRRKRDSFIFSKQEEQA DAALYKNLLHSKESSCDSSNEYMDMKPGVSYVVPTKTDKRRSARIDSYIERDVTPAIMEDDELALDLEDL LSFSYQVAKGMAFLASKNCIHRDLAARNILLTHGRITKICDFGLARDIRNDSNYVVKGNARLPVKWMAPE SIFNCVYTFESDVWSYGIFLWELFSLGSSPYPGMPVDSKFYKMIKEGFRMLSPEHAPAAMYEVMKTCWDA DPLKRPTFKQVVQLIEKQISDSSKHIYSNLANCNPNPENPVVVDHSVRVNSVGSSTSSTQPLLVHEDA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_022264 |
| ORF Size | 2934 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_022264.1, NP_071600.1 |
| RefSeq Size | 3816 bp |
| RefSeq ORF | 2937 bp |
| Locus ID | 64030 |
| MW | 109.3 kDa |
| Gene Summary | transmembrane receptor; deletion mutation is associated with anemia and deficiency in mast cells and melanocytes in the Ws mutant allele of rats [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN205111 | Kit (untagged ORF) - Rat v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (Kit), (10 ug) |
CNY 12,540.00 |
|
| RR205111L3 | Lenti ORF clone of Kit (Myc-DDK-tagged ORF) - Rat v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (Kit), (10 ug) |
CNY 14,250.00 |
|
| RR205111L4 | Lenti ORF clone of Kit (mGFP-tagged ORF) - Rat v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (Kit), (10 ug) |
CNY 15,680.00 |
