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Sqle (NM_017136) Rat Tagged ORF Clone
CAT＃: RR204970
- TrueORF®
Sqle (Myc-DDK-tagged ORF) - Rat squalene epoxidase (Sqle), (10 ug)
"NM_017136" in other vectors (3)
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CNY 7,320.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR204970 representing NM_017136
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGACTTTTCTTGGAATTGCTACTTTCACCTATTTTTATAAGAAATGCGGGGATGTCACCCTGGCCA ACAAGGAGCTCCTGCTGTGCGTACTGGTGTTCCTGTCGCTGGGCCTGGTGCTCTCCTACCGCTGTCGCCA TCGAAACGGGGGCCTCCTTGGCCGCCATCAGAGCGGCTCCCAGTTCGCTGCCTTCTCAGATATTCTCTCT GCTTTGCCTCTTATTGGCTTCTTCTGGGCCAAATCACCCCCTGAATCAGAAAAGAAAGAACAGCTGGAGT CCAAGAGGCGCAGAAAAGAAGTCAATCTGTCAGAGACGACGTTAACGGGAGCAGCTACCTCAGTATCGAC ATCATCTGTGACGGACCCAGAAGTGATCATCATAGGGTCTGGTGTACTTGGCTCTGCTTTGGCAACAGTA CTCTCTAGAGATGGAAGAACGGTGACAGTAATCGAGAGAGATTTAAAAGAGCCTGACAGAATACTTGGAG AGTGTCTGCAGCCCGGTGGCTATCGAGTTCTTCGAGAGCTGGGCCTTGGAGATACAGTAGAAAGTCTTAA TGCCCATCATATACACGGCTACGTAATTCATGACTGTGAAAGCAGGTCTGAAGTTCAAATTCCATACCCG GTGTCAGAAAACAACCAAGTACAGAGTGGGGTTGCTTTCCACCATGGCAAGTTCATCATGAGTCTCCGGA AAGCAGCTATGGCAGAGCCCAATGTAAAGTTTATAGAAGGTGTTGTGCTTCGGTTACTAGAGGAAGATGA TGCTGTGATTGGTGTGCAATACAAGGACAAGGAGACTGGGGACACCAAGGAGCTCCATGCCCCGCTCACT GTTGTTGCCGACGGGCTCTTCTCCAAGTTCAGGAAGAACCTCATCTCCAATAAAGTCTCTGTTTCCTCCC ACTTCGTTGGCTTCATTATGAAGGATGCACCACAGTTTAAAGCCAATTTCGCGGAGCTTGTTCTGGTCGA TCCCAGTCCAGTTCTCATCTACCAGATTTCACCCAGCGAAACTAGGGTGCTTGTCGATATTCGAGGAGAA TTGCCAAGAAACTTAAGAGAATACATGACAGAACAAATTTACCCACAGATACCTGATCACTTGAAGGAAT CATTTCTGGAGGCCTGTCAGAATGCTCGTCTGCGGACCATGCCAGCAAGCTTCCTTCCTCCTTCCTCAGT GAACAAACGAGGCGTCCTACTTCTGGGAGATGCGTATAACCTGAGGCATCCTCTTACTGGTGGAGGAATG ACAGTCGCTTTAAAAGATATAAAAATATGGAGACAACTGTTAAAAGATATTCCTGACCTTTATGATGATG CCGCTATTTTCCAGGCCAAAAAGTCATTCTTTTGGTCAAGAAAAAGGAGCCATTCCTTTGTTGTGAACGT GCTGGCTCAGGCGTTGTATGAACTATTTTCTGCTACAGATGATTCCTTGCGTCAGCTCCGAAAAGCTTGC TTTCTTTATTTTAAACTTGGTGGAGAATGTTTGACTGGTCCTGTTGGGTTGCTTTCTATATTGTCTCCTG ACCCTCTCCTATTGATTCGACACTTCTTCTCCGTTGCAGTCTATGCCACGTATTTCTGCTTCAAGTCAGA GCCATGGGCTACAAAACCTCGGGCTCTTTTCAGTAGTGGTGCTATACTGTACAAAGCGTGCTCTATAATA TTTCCTCTCATCTATTCAGAAATGAAGTATCTGGTTCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR204970 representing NM_017136
Red=Cloning site Green=Tags(s) MWTFLGIATFTYFYKKCGDVTLANKELLLCVLVFLSLGLVLSYRCRHRNGGLLGRHQSGSQFAAFSDILS ALPLIGFFWAKSPPESEKKEQLESKRRRKEVNLSETTLTGAATSVSTSSVTDPEVIIIGSGVLGSALATV LSRDGRTVTVIERDLKEPDRILGECLQPGGYRVLRELGLGDTVESLNAHHIHGYVIHDCESRSEVQIPYP VSENNQVQSGVAFHHGKFIMSLRKAAMAEPNVKFIEGVVLRLLEEDDAVIGVQYKDKETGDTKELHAPLT VVADGLFSKFRKNLISNKVSVSSHFVGFIMKDAPQFKANFAELVLVDPSPVLIYQISPSETRVLVDIRGE LPRNLREYMTEQIYPQIPDHLKESFLEACQNARLRTMPASFLPPSSVNKRGVLLLGDAYNLRHPLTGGGM TVALKDIKIWRQLLKDIPDLYDDAAIFQAKKSFFWSRKRSHSFVVNVLAQALYELFSATDDSLRQLRKAC FLYFKLGGECLTGPVGLLSILSPDPLLLIRHFFSVAVYATYFCFKSEPWATKPRALFSSGAILYKACSII FPLIYSEMKYLVH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017136 |
| ORF Size | 1719 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017136.2, NP_058832.1 |
| RefSeq Size | 2085 bp |
| RefSeq ORF | 1722 bp |
| Locus ID | 29230 |
| UniProt ID | P52020 |
| MW | 64 kDa |
| Gene Summary | enzyme that catalyzes sterol biosyntesis; may be the rare-limiting step in sterol biosynthesis pathway [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN204970 | Sqle (untagged ORF) - Rat squalene epoxidase (Sqle), (10 ug) |
CNY 7,320.00 |
|
| RR204970L3 | Lenti ORF clone of Sqle (Myc-DDK-tagged ORF) - Rat squalene epoxidase (Sqle), (10 ug) |
CNY 9,220.00 |
|
| RR204970L4 | Lenti ORF clone of Sqle (mGFP-tagged ORF) - Rat squalene epoxidase (Sqle), (10 ug) |
CNY 10,070.00 |
