Igf1r (NM_052807) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	IGF-1 receptor; IGFIRC; Igfr1; JTK13
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR204806 representing NM_052807 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGTCTGGCTCCGGAGGAGGGTCCCCGACCTCGCTGTGGGGGCTCGTGTTTCTCTCCGCCGCGCTCT CGCTCTGGCCGACGAGTGGAGAAATTTGTGGGCCCGGCATTGACATCCGCAACGACTATCAGCAGCTGAA GCGCCTGGAAAACTGCACGGTGATCGAGGGCTTCCTCCACATCCTGCTCATCTCCAAGGCCGAGGACTAC CGAAGCTACCGCTTCCCCAAGCTCACGGTCATCACCGAGTACTTGCTGCTGTTTCGAGTGGCCGGCCTCG AGAGCCTGGGAGACCTCTTCCCGAACCTCACAGTCATCCGTGGCTGGAAACTCTTCTACAATTACGCACT GGTCATCTTCGAGATGACCAATCTCAAGGATATTGGGCTTTATAATCTGAGGAACATTACTCGGGGGGCC ATCAGGATTGAGAAAAACGCTGACCTCTGTTACCTCTCCACCATAGACTGGTCTCTCATCTTGGATGCGG TGTCCAATAACTACATTGTGGGGAACAAGCCCCCAAAGGAATGTGGGGACCTGTGTCCAGGGACCTTGGA GGAGAAGCCCATGTGTGAGAAGACCACCATCAACAATGAGTACAACTACCGCTGCTGGACCACAAATCGC TGCCAGAAAATGTGCCCAAGTGTGTGTGGGAAGCGAGCCTGCACCGAGAACAATGAGTGCTGCCACCCGG AGTGCCTAGGCAGCTGCCACACACCGGACGACAACACAACCTGCGTGGCCTGCCGACACTACTACTACAA AGGCGTGTGCGTGCCTGCCTGCCCGCCTGGCACCTACAGGTTCGAGGGCTGGCGCTGTGTGGACCGGGAT TTCTGCGCCAACATCCCCAACGCCGAGAGCAGTGACTCAGATGGCTTCGTCATCCACGATGGCGAGTGCA TGCAGGAGTGTCCATCAGGCTTCATCCGCAACAGCACCCAGAGCATGTACTGTATCCCCTGTGAAGGCCC CTGCCCCAAGGTCTGCGGCGATGAAGAAAAGAAAACGAAAACCATCGATTCTGTGACGTCTGCCCAGATG CTCCAAGGGTGCACCATTTTGAAGGGCAATCTGCTTATTAACATCCGGCGAGGCAATAACATTGCCTCGG AATTGGAGAACTTCATGGGGCTCATCGAGGTGGTGACTGGCTACGTGAAGATCCGCCATTCCCATGCCTT GGTCTCCTTGTCCTTCCTGAAGAACCTTCGTCTCATCTTAGGAGAGGAGCAGCTAGAAGGGAACTACTCC TTCTATGTCCTGGACAACCAGAACTTGCAGCAGCTGTGGGACTGGAACCACCGGAACCTGACCGTCAGGT CAGGGAAAATGTACTTCGCTTTCAATCCCAAGCTGTGTGTCTCTGAAATTTACCGGATGGAGGAGGTGAC AGGAACAAAGGGACGGCAGAGCAAAGGAGACATAAACACCAGGAACAACGGAGAGCGAGCTTCCTGTGAA AGTGATGTTCTCCGTTTCACCTCCACCACCACCTGGAAGAACCGCATCATCATAACGTGGCACCGGTACC GGCCGCCGGACTACCGGGATCTCATCAGTTTCACAGTCTACTACAAGGAGGCACCCTTTAAAAACGTCAC GGAATACGACGGGCAGGATGCCTGTGGCTCCAACAGCTGGAACATGGTGGACGTGGACCTGCCTCCGAAC AAGGAGGGGGAGCCTGGCATTTTGCTGCATGGGCTGAAGCCCTGGACCCAGTATGCAGTCTATGTCAAGG CTGTGACCCTCACCATGGTGGAAAACGACCACATCCGTGGGGCCAAAAGTGAAATCTTGTACATTCGCAC CAACGCTTCAGTTCCTTCCATTCCTCTAGATGTCCTCTCGGCATCAAACTCCTCCTCTCAGCTGATCGTG AAGTGGAACCCCCCAACTCTGCCCAATGGTAACTTGAGTTACTACATTGTGAGGTGGCAGCGGCAGCCGC AGGATGGCTATCTGTTCCGGCACAACTACTGCTCCAAAGACAAAATACCCATCAGAAAGTACGCCGATGG TACCATCGATGTGGAGGAGGTGACAGAAAATCCCAAGACAGAAGTGTGCGGTGGTGATAAAGGGCCGTGC TGTGCCTGTCCTAAAACCGAAGCTGAGAAGCAGGCTGAGAAGGAGGAGGCTGAGTACCGTAAAGTCTTTG AGAATTTCCTTCACAACTCCATCTTTGTGCCCAGACCTGAGAGGAGGCGGAGAGATGTCCTGCAGGTGGC TAACACCACCATGTCCAGCCGAAGCAGGAACACCACGGTAGCTGACACCTACAATATCACAGACCCGGAA GAGTTCGAGACAGAATACCCTTTCTTTGAGAGCAGAGTGGATAACAAGGAGAGGACTGTCATTTCCAACC TCCGGCCTTTCACTCTGTACCGTATCGATATCCACAGCTGCAACCACGAGGCTGAGAAGCTGGGCTGCAG CGCCTCCAACTTTGTCTTTGCAAGAACCATGCCAGCAGAAGGAGCAGATGACATTCCTGGCCCAGTGACC TGGGAGCCAAGACCTGAAAACTCCATCTTTTTAAAGTGGCCAGAACCCGAGAACCCCAACGGATTGATTC TAATGTATGAAATAAAATACGGATCGCAAGTCGAGGATCAGCGGGAATGTGTGTCCAGACAGGAGTACAG GAAGTATGGAGGGGCCAAACTTAACCGTCTAAACCCAGGGAACTATACGGCCCGGATTCAGGCTACCTCC CTCTCTGGGAATGGGTCGTGGACAGATCCTGTGTTCTTCTATGTCCCAGCCAAAACAACGTATGAGAATT TCATGCATCTGATCATTGCTCTGCCGGTTGCCATCCTGCTGATTGTGGGGGGCCTGGTAATCATGCTGTA TGTCTTCCATAGAAAGAGGAATAACAGCAGATTGGGCAACGGGGTGCTGTACGCCTCTGTGAACCCCGAG TATTTCAGCGCAGCTGATGTGTACGTGCCTGATGAATGGGAGGTAGCTCGGGAGAAGATCACCATGAACC GGGAGCTCGGACAAGGGTCCTTCGGGATGGTCTATGAAGGAGTGGCCAAGGGCGTGGTCAAGGACGAGCC TGAAACCAGAGTGGCCATCAAGACAGTGAATGAGGCTGCAAGTATGCGTGAGAGAATTGAGTTTCTCAAC GAGGCCTCAGTGATGAAGGAGTTCAACTGTCACCATGTGGTCCGGTTGCTGGGTGTAGTATCCCAAGGCC AGCCCACCCTGGTCATCATGGAACTAATGACACGTGGCGATCTCAAAAGTTATCTCCGGTCTCTAAGGCC AGAGGTGGAGAATAATCTAGTCCTGATTCCTCCGAGCTTAAGCAAGATGATCCAGATGGCTGGAGAGATT GCAGATGGCATGGCCTACCTCAATGCCAACAAGTTCGTCCACAGAGACCTGGCTGCTCGGAACTGCATGG TAGCTGAAGATTTCACAGTCAAAATTGGAGATTTTGGTATGACACGAGACATCTACGAGACGGACTACTA CCGGAAAGGCGGGAAGGGCTTGCTGCCTGTGCGCTGGATGTCTCCCGAGTCCCTCAAGGATGGCGTCTTC ACCACTCATTCCGATGTCTGGTCCTTTGGGGTCGTCCTCTGGGAGATCGCCACTCTGGCTGAGCAGCCGT ACCAGGGCCTGTCCAACGAGCAAGTTCTTCGTTTCGTCATGGAGGGCGGCCTTCTGGACAAGCCGGATAA CTGCCCCGATATGCTGTTTGAACTTATGCGCATGTGCTGGCAGTACAACCCCAAGATGCGGCCCTCCTTC CTGGAGATCATCGGAAGCATCAAGGATGAGATGGAGCCCAGTTTCCAGGAGGTCTCCTTCTACTACAGCG AGGAGAACAAGCCTCCAGAGCCGGAGGAGCTGGAGATGGAGCTGGAGCTGGAGCCCGAGAACATGGAGAG CGTCCCGCTGGACCCTTCGGCCTCCTCAGCCTCCCTGCCTCTGCCTGAAAGACACTCAGGACACAAGGCT GAGAACGGCCCTGGCGTGCTGGTTCTCCGTGCCAGTTTTGATGAGAGACAGCCTTACGCTCACATGAATG GGGGACGCGCCAACGAGAGGGCCTTGCCTCTGCCCCAGTCCTCAACCTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR204806 representing NM_052807 Red=Cloning site Green=Tags(s) MKSGSGGGSPTSLWGLVFLSAALSLWPTSGEICGPGIDIRNDYQQLKRLENCTVIEGFLHILLISKAEDY RSYRFPKLTVITEYLLLFRVAGLESLGDLFPNLTVIRGWKLFYNYALVIFEMTNLKDIGLYNLRNITRGA IRIEKNADLCYLSTIDWSLILDAVSNNYIVGNKPPKECGDLCPGTLEEKPMCEKTTINNEYNYRCWTTNR CQKMCPSVCGKRACTENNECCHPECLGSCHTPDDNTTCVACRHYYYKGVCVPACPPGTYRFEGWRCVDRD FCANIPNAESSDSDGFVIHDGECMQECPSGFIRNSTQSMYCIPCEGPCPKVCGDEEKKTKTIDSVTSAQM LQGCTILKGNLLINIRRGNNIASELENFMGLIEVVTGYVKIRHSHALVSLSFLKNLRLILGEEQLEGNYS FYVLDNQNLQQLWDWNHRNLTVRSGKMYFAFNPKLCVSEIYRMEEVTGTKGRQSKGDINTRNNGERASCE SDVLRFTSTTTWKNRIIITWHRYRPPDYRDLISFTVYYKEAPFKNVTEYDGQDACGSNSWNMVDVDLPPN KEGEPGILLHGLKPWTQYAVYVKAVTLTMVENDHIRGAKSEILYIRTNASVPSIPLDVLSASNSSSQLIV KWNPPTLPNGNLSYYIVRWQRQPQDGYLFRHNYCSKDKIPIRKYADGTIDVEEVTENPKTEVCGGDKGPC CACPKTEAEKQAEKEEAEYRKVFENFLHNSIFVPRPERRRRDVLQVANTTMSSRSRNTTVADTYNITDPE EFETEYPFFESRVDNKERTVISNLRPFTLYRIDIHSCNHEAEKLGCSASNFVFARTMPAEGADDIPGPVT WEPRPENSIFLKWPEPENPNGLILMYEIKYGSQVEDQRECVSRQEYRKYGGAKLNRLNPGNYTARIQATS LSGNGSWTDPVFFYVPAKTTYENFMHLIIALPVAILLIVGGLVIMLYVFHRKRNNSRLGNGVLYASVNPE YFSAADVYVPDEWEVAREKITMNRELGQGSFGMVYEGVAKGVVKDEPETRVAIKTVNEAASMRERIEFLN EASVMKEFNCHHVVRLLGVVSQGQPTLVIMELMTRGDLKSYLRSLRPEVENNLVLIPPSLSKMIQMAGEI ADGMAYLNANKFVHRDLAARNCMVAEDFTVKIGDFGMTRDIYETDYYRKGGKGLLPVRWMSPESLKDGVF TTHSDVWSFGVVLWEIATLAEQPYQGLSNEQVLRFVMEGGLLDKPDNCPDMLFELMRMCWQYNPKMRPSF LEIIGSIKDEMEPSFQEVSFYYSEENKPPEPEELEMELELEPENMESVPLDPSASSASLPLPERHSGHKA ENGPGVLVLRASFDERQPYAHMNGGRANERALPLPQSSTC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_052807
ORF Size	4110 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_052807.2, NP_434694.1
RefSeq Size	4693 bp
RefSeq ORF	4113 bp
Locus ID	25718
UniProt ID	P24062
MW	155.4 kDa
Gene Summary	receptor for Igf-1; involved in induction of cell cycle progression and survival in many cell types [RGD, Feb 2006]

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