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Plk4 (NM_001107669) Rat Tagged ORF Clone
CAT＃: RR203318
- TrueORF®
Plk4 (Myc-DDK-tagged ORF) - Rat polo-like kinase 4 (Drosophila) (Plk4), (10 ug)
"NM_001107669" in other vectors (3)
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CNY 11,210.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR203318 representing NM_001107669
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGTGCATCGGAGAGAGGATCGAGGACTTTAAGGTTGGAAATCTACTTGGTAAAGGATCATTTG CTGGTCTCTATAATTACTTTGAAGATAACAATTACGTATACCTGGTATTAGAAATGTGCCACAACGGAGA AATGAACAGGTACCTAAAGAACAGAATGAAGCCCAGGCACTTCATGCACCAGATCATCACAGGAATGCTG TATCTTCATTCTCACGGCATACTACACCGCGACCTCACACTCTCTAACATCTTACTTACACGGAATATGA ACATAAAAATTGCTGACTTTGGACTAGCAACACAATTGAAAATGCCACATGAGAAGCATTACACCCTGTG TGGAACCCCTAATTATATTTCACCAGAAATTGCAACTCGAAGTGCACATGGACTTGAATCTGATATTTGG TCATTGGGCTGTATGTTTTATACATTACTTATTGGAAGACCACCTTTTGACACTGATACAGTCAAAAACA CATTGAACAAAGTAGTATTGGCAGATTATGAAATGCCAGCCTTTTTGTCACGAGAGGCCCAGGACCTTAT CCACCAATTACTTCGTAGAAACCCTGCAGATCGGTTAAGTCTGTCTTCTGTGTTGGACCATCCTTTTATG TCACGAAATCCTTCAACAAAGAGTAAAGACTTAGGGACTGTAGAGGACTCAATGGATAGTGGGCATGCCA CACTTTCCACAACAATTACAGCTTCTTCCGGAACCAGTTTGAGTGGTAGCCTACTTGACAGAAGAAGACT TTTGGTTGGTCAACCACTTCCAAATAAAATTACTGTATTTCAAAAAAATAAAAATTCAAGTGACTTTTCT TCAGGAGATGGAAGTAATTTTTGTACTCAATGGGGAAATCCAGAACAAGAAGCTAATAATAGGGGGCGGG GAAGAGTAATTGAAGATGCAGAAGAAAGGCCACATTCTCGATACCTTCGCAGAGCTCATTCCTCTGATAG ATCCAACCCTTCTAATCAGTCTCGAGCAAAAACATACTCAATAGAGCGGTGTCACTCAGTAGAAATGCTT TCAAAGCCCAGAAGATCATCACTGGATGAAACTAAACACAGTTCCAATCATCATTGTCTAGGGAAAACTC CTTTTCCATTTGCAGACCAGACACCTCAGATGGAGATAGTACAACAGTGGTTTGGGAATCTGCAAATGAA TGGAGAAACTAGTGAGCACAACACCATTAGCCCAAACAGAGATTTCCAGGACTATCCTGATGTGCAGGAC ACATTAAGAAATACTTGGACTGACACAAGAGCCAGCAAGAATTCCGATAATTCTGCTAACGTTCATCCTG CAAAGCAGCTGAGTACCATGAAATACATGACTGCACATCACCATAAACCTGAGATCATGCAGCAAGAGCT GGCCATACATCCTCATTCTGAACAAAACAAGAGTAGAAGTATGGAGTCAACATTGGGTTACAGGAAACCT ACCTTAAGAAGCATTACCTCTCCTTTGGTTGCTCACAGACTAAAACCAATCAGACAGAAAACCAAAAAGG CTGTGGTGAGCATCCTTGATTCAGAGGAGGTGTGTGTGGAGCTTCTAAAAGAGTGTACATCTGAAGGACA TGTGAAAGAAGTTCTTCAGATATCTAGTGATGGGACTACGATCACCGTTTATTACCCAAATGATGGAAGA GGCTTTCCTCTTGCTGATAGACCACCCTTGCCTACTGACAACATCAGCAGGTACAGCTTTGACAGTTTAC CAGAAAAATACTGGCGGAAATATCAGTATGCTTCCAGATTCATTCAGCTTGTAAGGTCCAAAACTCCCAA AATCACTTACTTTACAAGATATGCTAAATGTATTTTGATGGAAAATTCTCCTGGTGCTGATTTTGAAGTT TGGTTTTATGATGGAGCCAAAATACATAAAACAGAAGATGTAATTCACATAATTGAAAAAACTGGGTTAT CTTACACTTTAAAAAATGAGAATGACTTTACCAGCTTGAAAGAGGAAGTAAAAATCTATATGGACCATGC CAATGAGGGTCACCGTACTTGCTTGGCACTGGAATCTGTAATCTCTGAAGAGGAAAAAAGAAGCAGGGGC TCTTCATTCTTCCCAATAATTGTAGGAAGAAAACCTGGTACTACTAGTTCACCTAAAGCCTTATCACCAC CTCCTGTGGACCCAGGCTACTCAAAGGGAGAGCAAGCATCATCAAGCAGATTGAGCGCGAATAGTGCGGC TTTCCCAACACAGACACCAGTACTCAGTCCTTCTGCTGTGACAGTGGAAGGACCTGGCCAAACAGCAGCT ACTACAGGAACAAGCATCTCTTCAAGTCTTCCTAAATCAGCACAGCTTTTGAAATCTGTTTTTGTGAAAA ATGTTGGTTGGGCTACACAGCTAACTAGTGGAGCTGTGTGGGTTCAGTTTAATGATGGGTCACAGTTGGT TGTCCAGGCAGGAGTATCTTCCATCAGTTATACATCACCAGACGGTCAGACAACTAGGTATGGAGAAAAT GAAAAATTGCCTGAATACATCAAACAGAAATTGCAATGTCTTTCTTCCATCCTTCTGATGTTTTCTAATC CAACTCCTAGTTTTCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR203318 representing NM_001107669
Red=Cloning site Green=Tags(s) MAACIGERIEDFKVGNLLGKGSFAGLYNYFEDNNYVYLVLEMCHNGEMNRYLKNRMKPRHFMHQIITGML YLHSHGILHRDLTLSNILLTRNMNIKIADFGLATQLKMPHEKHYTLCGTPNYISPEIATRSAHGLESDIW SLGCMFYTLLIGRPPFDTDTVKNTLNKVVLADYEMPAFLSREAQDLIHQLLRRNPADRLSLSSVLDHPFM SRNPSTKSKDLGTVEDSMDSGHATLSTTITASSGTSLSGSLLDRRRLLVGQPLPNKITVFQKNKNSSDFS SGDGSNFCTQWGNPEQEANNRGRGRVIEDAEERPHSRYLRRAHSSDRSNPSNQSRAKTYSIERCHSVEML SKPRRSSLDETKHSSNHHCLGKTPFPFADQTPQMEIVQQWFGNLQMNGETSEHNTISPNRDFQDYPDVQD TLRNTWTDTRASKNSDNSANVHPAKQLSTMKYMTAHHHKPEIMQQELAIHPHSEQNKSRSMESTLGYRKP TLRSITSPLVAHRLKPIRQKTKKAVVSILDSEEVCVELLKECTSEGHVKEVLQISSDGTTITVYYPNDGR GFPLADRPPLPTDNISRYSFDSLPEKYWRKYQYASRFIQLVRSKTPKITYFTRYAKCILMENSPGADFEV WFYDGAKIHKTEDVIHIIEKTGLSYTLKNENDFTSLKEEVKIYMDHANEGHRTCLALESVISEEEKRSRG SSFFPIIVGRKPGTTSSPKALSPPPVDPGYSKGEQASSSRLSANSAAFPTQTPVLSPSAVTVEGPGQTAA TTGTSISSSLPKSAQLLKSVFVKNVGWATQLTSGAVWVQFNDGSQLVVQAGVSSISYTSPDGQTTRYGEN EKLPEYIKQKLQCLSSILLMFSNPTPSFQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001107669 |
| ORF Size | 2607 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001107669.1, NP_001101139.1 |
| RefSeq Size | 3299 bp |
| RefSeq ORF | 2610 bp |
| Locus ID | 310344 |
| MW | 97.5 kDa |
| Gene Summary | Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CCP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Phosphorylates 'Ser-151' of FBXW5 during the G1/S transition, leading to inhibit FBXW5 ability to ubiquitinate SASS6. Its central role in centriole replication suggests a possible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Also involved in deuterosome-mediated centriole amplification in multiciliated that can generate more than 100 centrioles. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Phosphorylates CDC25C and CHEK2. Required for the recruitment of STIL to the centriole and for STIL-mediated centriole amplification (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN203318 | Plk4 (untagged ORF) - Rat polo-like kinase 4 (Drosophila) (Plk4), (10 ug) |
CNY 11,210.00 |
|
| RR203318L3 | Lenti ORF clone of Plk4 (Myc-DDK-tagged ORF) - Rat polo-like kinase 4 (Drosophila) (Plk4), (10 ug) |
CNY 12,920.00 |
|
| RR203318L4 | Lenti ORF clone of Plk4 (mGFP-tagged ORF) - Rat polo-like kinase 4 (Drosophila) (Plk4), (10 ug) |
CNY 14,160.00 |
