United States
Germany
Japan
United Kingdom
China
Chst15 (NM_173310) Rat Tagged ORF Clone
CAT＃: RR202867
- TrueORF®
Chst15 (Myc-DDK-tagged ORF) - Rat B cell RAG associated protein (Galnac4s-6st), (10 ug)
"NM_173310" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Galnac4s-6st; GalNAc4S6ST |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR202867 representing NM_173310
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCACTGCATTAATTGCTGCATACAGCTGTTCCCCGAAGACGCACACAAACAGCAGGTTGCCTGCC AAGGAGGCCCCCATCACAGTCATCAGGCGTGCCCCAGTTGCAAAGGAGAAGACAAAATTCTGTTCCGGGT GGACAGTAAGCAGATGAACTTGCTTGCTGTTCTCGAAGTGAGGACTGAGGGCAACGAAAACTGGGGCGGG TTTTTGCGCTTCAGGAAAGGGAAGCGATGTAGCCTGGTCTTTGGATTGATAATAATGACCTTGGTGATGG CTTCTTACATCCTTTCCGGGGCTCATCAGGAACTTCTAATTTCATCCCCTTTCCATTATGGGGGCTTTCC CAGCAACCCCAGCGTGATGGACAGTGAGAGCCCTAGTGATGTGAAAGAGCATCACTACCAGCCTTCGGTA AATAACATCTCCTATGTGAAGGATTATCCCAACATTAAACTGATTATTGACAGCATCGCTGCCAGGAATG AGTTCACAACCAGGCAGCTCCCGGACTTACAAGATCTCAAGAGACAAGAGTTGCACATGTTTTCTGTGAT CCCCAATAAATTCCTTCCGACTAGCAAGAGCCCTTGTTGGTATGAGGAATTCTCCGGCAGGAACACCACT GACCCCTACCTGACCAATTCCTACGTGCTCTACTCCAAGCGCTTCCGCTCTACCTTTGACACTCTGCGAA AGGCTTTCTGGGGCCACCTGTCCCATGTGCATGGCAAGCATTTCCGCCTGCGCTGCCTGCCACACTTCTA CATCATTGGGCAGCCCAAGTGCGGAACCACTGACCTCTATGACCGCCTTCGGCTGCATCCAGAAGTGAAA TTCTCAGCCATCAAGGAGCCCCACTGGTGGACCCGGAAGCGCTTTGGAATTGTCCGCCTGAGGGATGGGC TACGAGACCGCTACCCTGTGGAAGATTACCTGGACCTCTTTGACTTGGCTGCACACCAGATCCATCAAGG ACTGCAGGCTGCCTCTGCAGAGCAGACGAGCAAGATGAATAGGATCATTATCGGAGAGGCCAGCGCCTCT ACAATGTGGGATAACAATGCCTGGACCTTCTTCTATGACAACAGCACAGACGGCGAGCCACCGTTCCTGA CCCAAGACTTCATCCATGCCTTTCAGCCTGAAGCCAAGCTCCTTGTCATGCTCAGGGATCCCGTGGAGAG GTTGTACTCAGACTATCTCTACTTTGCAAGTTCAAATAAATCCGCCGATGACTTCCACGAGAAGGTGACG GAGGCTCTGCAGCTGTTTGAAAATTGCATGCTGGATTATTCACTGCGCGCCTGCGTCTACAACAACACCC TGAACAATGCCATGCCCGTGAGGCTCCAGGTCGGTCTGTATGCTGTGTATCTCCTGGACTGGCTCACTGT CTTCAGTAAGGAGCAGTTCCTCATTCTACGTCTGGAAGACCATGCATCCAATGTCAAGTACACCATGCAC AAGGTCTTCCAGTTTCTAAACCTGGGGCCTCTAAGTGAGAAGCAAGAAGCTTTGATGACCAAGAGCCCCG CGTCCAACACACGGCGTCCTGAGGATCGTAGTCTGGGACCCATGTGGCCGATCACTCAGAAGATCCTGCG GGACTTCTACGGGCCTTTCAACACAAGGCTGGCACAGGTCCTGGATGATGAGGCGTTTGCGTGGAAGACG ACG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR202867 representing NM_173310
Red=Cloning site Green=Tags(s) MRHCINCCIQLFPEDAHKQQVACQGGPHHSHQACPSCKGEDKILFRVDSKQMNLLAVLEVRTEGNENWGG FLRFRKGKRCSLVFGLIIMTLVMASYILSGAHQELLISSPFHYGGFPSNPSVMDSESPSDVKEHHYQPSV NNISYVKDYPNIKLIIDSIAARNEFTTRQLPDLQDLKRQELHMFSVIPNKFLPTSKSPCWYEEFSGRNTT DPYLTNSYVLYSKRFRSTFDTLRKAFWGHLSHVHGKHFRLRCLPHFYIIGQPKCGTTDLYDRLRLHPEVK FSAIKEPHWWTRKRFGIVRLRDGLRDRYPVEDYLDLFDLAAHQIHQGLQAASAEQTSKMNRIIIGEASAS TMWDNNAWTFFYDNSTDGEPPFLTQDFIHAFQPEAKLLVMLRDPVERLYSDYLYFASSNKSADDFHEKVT EALQLFENCMLDYSLRACVYNNTLNNAMPVRLQVGLYAVYLLDWLTVFSKEQFLILRLEDHASNVKYTMH KVFQFLNLGPLSEKQEALMTKSPASNTRRPEDRSLGPMWPITQKILRDFYGPFNTRLAQVLDDEAFAWKT T TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_173310 |
| ORF Size | 1683 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_173310.3, NP_775432.3 |
| RefSeq Size | 1701 bp |
| RefSeq ORF | 1686 bp |
| Locus ID | 286974 |
| UniProt ID | Q8CHI9 |
| MW | 65 kDa |
| Gene Summary | Sulfotransferase that transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4,6-SO(4)) repeating units. It also transfers sulfate to a unique non-reducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. May also act as a B-cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and/or regulation of B-cell-specific RAG expression; however such results are unclear in vivo (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN202867 | Chst15 (untagged ORF) - Rat B cell RAG associated protein (Galnac4s-6st), (10 ug) |
CNY 7,130.00 |
|
| RR202867L3 | Lenti ORF clone of Chst15 (Myc-DDK-tagged ORF) - Rat B cell RAG associated protein (Galnac4s-6st), (10 ug) |
CNY 9,120.00 |
|
| RR202867L4 | Lenti ORF clone of Chst15 (mGFP-tagged ORF) - Rat B cell RAG associated protein (Galnac4s-6st), (10 ug) |
CNY 9,980.00 |
