United States
Germany
Japan
United Kingdom
China
Myog (NM_017115) Rat Tagged ORF Clone
CAT＃: RR202859
- TrueORF®
Myog (Myc-DDK-tagged ORF) - Rat myogenin (Myog), (10 ug)
"NM_017115" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR202859 representing NM_017115
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTGTATGAAACATCCCCCTATTTCTACCAGGAGCCCCACTTCTATGACGGGGAAAACTACCTTC CTGTCCACCTTCAGGGCTTCGAGCCCCCGGGCTATGAGCGGACCGAGCTCAGCTTAAGCCCGGAAGCCCG AGGGCCCCTAGAAGAGAAGGGACTGGGGACCCCTGAGCATTGTCCAGGCCAGTGCCTGCCCTGGGCGTGT AAGGTGTGTAAGAGGAAGTCTGTGTCTGTGGACCGGCGGAGGGCAGCCACACTGAGGGAGAAGCGCAGGC TCAAGAAAGTGAATGAGGCCTTCGAGGCTCTGAAGAGAAGCACCCTGCTCAACCCCAACCAGCGGCTGCC TAAGGTGGAGATCCTGCGCAGTGCCATCCAGTACATTGAGCGCCTACAGGCCTTGCTCAGCTCCCTCAAC CAGGAGGAGCGCGATCTCCGCTACCGAGGCGGGGGCGGGCCCCAGCCGGTGGTACCCAGTGAATGCAACT CCCACAGCGCCTCCTGCAGTCCGGAGTGGGGCAATGCACTGGAGTTTGGTCCCAACCCAGGAGATCATTT GCTCGCAGCTGACCCTACAGGTGCCCACAACCTGCACTCCCTTACGTCCATCGTGGACAGCATCACGGTG GAGGATATGTCTGTCACCTTCCCAGATGAAACCATGCCCAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR202859 representing NM_017115
Red=Cloning site Green=Tags(s) MELYETSPYFYQEPHFYDGENYLPVHLQGFEPPGYERTELSLSPEARGPLEEKGLGTPEHCPGQCLPWAC KVCKRKSVSVDRRRAATLREKRRLKKVNEAFEALKRSTLLNPNQRLPKVEILRSAIQYIERLQALLSSLN QEERDLRYRGGGGPQPVVPSECNSHSASCSPEWGNALEFGPNPGDHLLAADPTGAHNLHSLTSIVDSITV EDMSVTFPDETMPN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017115 |
| ORF Size | 672 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017115.2, NP_058811.2 |
| RefSeq Size | 1448 bp |
| RefSeq ORF | 675 bp |
| Locus ID | 29148 |
| UniProt ID | P20428 |
| MW | 25.1 kDa |
| Gene Summary | This gene belongs to the myogenic factor subfamily of the basic helix-loop-helix (BHLH) family of transcription factors. The protein encoded by this gene is a muscle-specific skeletal myogenin, which acts in concert with other family members to regulate myogenesis. The protein plays an essential role in the conversion of undifferentiated cells to myoblasts and functional skeletal muscle. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN202859 | Myog (untagged ORF) - Rat myogenin (Myog), (10 ug) |
CNY 3,990.00 |
|
| RR202859L3 | Lenti ORF clone of Myog (Myc-DDK-tagged ORF) - Rat myogenin (Myog), (10 ug) |
CNY 6,080.00 |
|
| RR202859L4 | Lenti ORF clone of Myog (mGFP-tagged ORF) - Rat myogenin (Myog), (10 ug) |
CNY 6,650.00 |
