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Pomgnt1 (NM_001007747) Rat Tagged ORF Clone
CAT＃: RR202765
- TrueORF®
Pomgnt1 (Myc-DDK-tagged ORF) - Rat protein O-linked mannose beta1,2-N-acetylglucosaminyltransferase (Pomgnt1), (10 ug)
"NM_001007747" in other vectors (3)
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CNY 8,550.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MGC94463 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR202765 representing NM_001007747
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGACTGGAAGCCTAGTCCCCTCATCAAGCCCTTTGGGGCTAGGAAGAAACGGAGCTGGTATCTTA CCTGGAAGTATAAACTGACAAACCAGCGGGCCCTGCGAAGATTCTGTCAGACAGGAGCTGTGCTTTTCCT GCTGGTGACTGTCATTGTGAACATCAAGTTGATCCTAGACACTCGGAGAGCCATCAGCGAGGCCAATGAA GACCCTGAACCAGAGCAAGACTACGATGAGGCCCTAGGACGCTTAGAGTCCCCTCGGCACAGAGGCAGCA GTCCCCGACGCGTGCTGGATGTGGAAGTATACTCCAGTAGAAGCAAAGTGTATGTGGCGGTAGATGGCAC TACGGTGCTAGAGGATGAGGCCCGGGAGCAGGGCCGAGGCATCCATGTCATCGTCCTCAACCAGGCCACG GGCCACGTGATGGCAAAGCGTGTGTTTGACACGTACTCTCCTCATGAGGATGAGGCCATGGTGCTATTCC TCAACATGGTGGCGCCCGGCCGTGTACTGATCTGCACAGTTAAGGATGAGGGCTCCTTCCACCTCAAGGA CACAGCAAAGGCTCTGCTGAGGAGCCTGGGAAGCCAGGCAGGCCCTGCCCTTGGCTGGAGGGACACCTGG GCCTTCGTGGGACGAAAAGGAGGTCCTGTTCTTGGCGAGAAGCATTCTAAATCTCCTGCCCTCTCCTCCT GGGGTGACCCAGTCCTGCTGAAGACAGATGTGCCACTCAGCTCAGCTGAAGAGGCTGAGTGTCACTGGGC AGATACAGAGTTGAACCGCCGCCGCAGGCGCTTCTGCAGCAAAGTCGAGGGCTACGGGAGCGTGTGCAGC TGCAAGGACCCAACACCTATTGAGTTCAGCCCTGACCCACTTCCAGACAATAAAGTCCTCAATGTGCCTG TGGCTGTCATTGCAGGGAACCGGCCCAATTACCTGTACAGGATGCTGCGCTCTCTGCTGTCAGCCCAAGG GGTATCTCCACAGATGATAACAGTCTTCATTGATGGCTACTATGAGGAGCCAATGGATGTGGTGGCACTG TTTGGTCTGAGAGGCATCCAGCACACTCCCATCAGCATCAAGAACGCCCGTGTGTCTCAGCACTACAAGG CCAGCCTCACTGCCACTTTCAACCTGTTTCCGGAGGCCAAGTTTGCTGTGGTTCTGGAAGAGGACCTGGA CATCGCTGTGGACTTTTTCAGTTTCCTGAGCCAGTCCATCCACCTGCTGGAGGAAGATGACAGCCTGTAC TGCATCTCTGCTTGGAATGACCAGGGGTATGAACACACAGCTGAGGATCCAGCACTACTGTACCGTGTGG AGACCATGCCTGGGTTGGGATGGGTGCTCAGGAAATCTCTATACAAGGAGGAGCTTGAGCCCAAGTGGCC CACACCAGAAAAGCTGTGGGACTGGGACATGTGGATGCGGATGCCGGAACAGCGCCGTGGCCGTGAGTGC ATCATCCCGGATGTTTCCCGATCCTACCACTTTGGCATCGTGGGCCTCAACATGAATGGCTACTTTCATG AGGCCTACTTCAAGAAGCACAAGTTCAACACAGTCCCAGGTGTCCAGCTCAGGAATGTAGACAGTCTGAA GAAAGAAGCTTATGAAGTGGAAATTCACAGGCTGCTCAGTGAGGCCGAGGTTTTGGACCACAGCAAGGAC CCTTGTGAAGACTCCTTCCTGCCAGACACAGAGGGCCATACCTATGTGGCATTTATCCGGATGGAGAAAG ATGATGACTTCACCACCTGGACTCAGCTTGCCAAGTGCCTCCACATTTGGGATCTGGACGTTCGTGGCAA CCACCGGGGCCTGTGGAGATTATTTCGGAAGAAGAACCACTTTTTGGTGGTGGGGGTCCCAGCCTCCCCT TACTCAGTGAAGAAGCCACCCTCAGTCACCCCTATTTTCCTGGAGCCACCCCCAAAGGAAGAAGGAGCCC CAGGGGCTGCTGAACAAACA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RR202765 representing NM_001007747
Red=Cloning site Green=Tags(s) MDDWKPSPLIKPFGARKKRSWYLTWKYKLTNQRALRRFCQTGAVLFLLVTVIVNIKLILDTRRAISEANE DPEPEQDYDEALGRLESPRHRGSSPRRVLDVEVYSSRSKVYVAVDGTTVLEDEAREQGRGIHVIVLNQAT GHVMAKRVFDTYSPHEDEAMVLFLNMVAPGRVLICTVKDEGSFHLKDTAKALLRSLGSQAGPALGWRDTW AFVGRKGGPVLGEKHSKSPALSSWGDPVLLKTDVPLSSAEEAECHWADTELNRRRRRFCSKVEGYGSVCS CKDPTPIEFSPDPLPDNKVLNVPVAVIAGNRPNYLYRMLRSLLSAQGVSPQMITVFIDGYYEEPMDVVAL FGLRGIQHTPISIKNARVSQHYKASLTATFNLFPEAKFAVVLEEDLDIAVDFFSFLSQSIHLLEEDDSLY CISAWNDQGYEHTAEDPALLYRVETMPGLGWVLRKSLYKEELEPKWPTPEKLWDWDMWMRMPEQRRGREC IIPDVSRSYHFGIVGLNMNGYFHEAYFKKHKFNTVPGVQLRNVDSLKKEAYEVEIHRLLSEAEVLDHSKD PCEDSFLPDTEGHTYVAFIRMEKDDDFTTWTQLAKCLHIWDLDVRGNHRGLWRLFRKKNHFLVVGVPASP YSVKKPPSVTPIFLEPPPKEEGAPGAAEQT SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001007747 |
| ORF Size | 1980 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001007747.1, NP_001007748.1 |
| RefSeq Size | 2745 bp |
| RefSeq ORF | 1983 bp |
| Locus ID | 362567 |
| UniProt ID | Q5XIN7 |
| MW | 75.1 kDa |
| Gene Summary | Participates in O-mannosyl glycosylation by catalyzing the addition of N-acetylglucosamine to O-linked mannose on glycoproteins. Catalyzes the synthesis of the GlcNAc(beta1-2)Man(alpha1-)O-Ser/Thr moiety on alpha-dystroglycan and other O-mannosylated proteins, providing the necessary basis for the addition of further carbohydrate moieties. Is specific for alpha linked terminal mannose.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN202765 | Pomgnt1 (untagged ORF) - Rat protein O-linked mannose beta1,2-N-acetylglucosaminyltransferase (Pomgnt1), (10 ug) |
CNY 8,550.00 |
|
| RR202765L3 | Lenti ORF clone of Pomgnt1 (Myc-DDK-tagged ORF) - Rat protein O-linked mannose beta1,2-N-acetylglucosaminyltransferase (Pomgnt1), (10 ug) |
CNY 10,260.00 |
|
| RR202765L4 | Lenti ORF clone of Pomgnt1 (mGFP-tagged ORF) - Rat protein O-linked mannose beta1,2-N-acetylglucosaminyltransferase (Pomgnt1), (10 ug) |
CNY 11,310.00 |
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