Synj2 (NM_001113371) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR200255 representing NM_001113371 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCTGAGCAAAGGGCTGCGACTGCTGGCGCGGCTGGACCCCACCGGCCCGAGCAGCGTGCTGCTGG AGGCGCGGGGCCGCGGCGACTGTCTACTCTTTGAGGCCGGCGCGGTGGCCACGCTGGCTCCGGAAGAGAA GGAAGTCATTAAAGGACTGTATGGGAAGCCGACGGATGCCTATGGCTGCCTCGGGGAGCTGAGTTTAAAA TCCGGTGGCGTCCCCTTGAGCTTTCTGGTGTTGGTGACAGGCTGCACGTCAGTGGGCAGAATTCCAGATG CAGAGATCTACAAAATCACTGGCACTGAGTTTTACCCCCTGCAGGAAGAGGCCAAGGAAGAGGACCGCCT GCCAGCCTTGAAGAAAATCCTGAGTTCAGGGGTGTTCTATTTCGCATGGCCCAATGATGGCGCCTGCTTC GATCTGACCATCAGGGCTCAGAAACAGGGTGATGACTGCTCTGAATGGGGGACCTCCTTCTTCTGGAACC AGCTATTGCATGTGCCTCTGCGGCAGCACCAGGTGAACTGTCATGACTGGTTGCTGAAAGTCATCTGTGG GGTGGTGACCATCCGCACGGTTTATGCCTCCCACAAGCAGGCCAAGGCCTGTCTCATCTCTCGCATCAGC TGTGAACGCGCAGGTGCTCGCTTCCTCACCCGGGGTGTGAACGACGATGGCCACGTGTCCAACTTTGTGG AGACAGAGCAGGCGATTTACATGGATGATGGAGTGTCGTCCTTTGTCCAGATCCGAGGCTCCGTTCCGCT GTTCTGGGAGCAGCCAGGGCTCCAGGTTGGCTCCCATCATCTGAGACTGCACAGAGGCCTAGAGGCCAAC GCCCCTGCTTTTGAAAGGCACATGGTGCTTCTGAAGGAGCAGTACGGGCAGCAGGTGGTGGTGAACCTGC TGGGAAGCAGAGGCGGTGAGGAGGTGCTCAACAGAGCCTTCAAGAAGTTGCTCTGGGCTTCTTGCCACGC AGGCGACACGCCTATGATAAATTTTGACTTCCATCAGTTTGCCAAAGGTAGGAAGCTAGAGAAATTGGAG AACCTGTTGAGACCTCAGTTAAAACTACACTGGGATGACTTTGGCGTATTTGCGAAGGGCGAGAATGTAA GTCCACGGTTTCAGAAAGGCACTCTGCGGATGAACTGTCTCGACTGTCTGGACAGAACCAACACTGTACA GTGCTTCATTGCTCTTGAGGTGCTTCATCTGCAGCTTGAGAGCCTAGGGCTGAATTCCAAGCCCATCACT GACCGCTTCGTGGAGTCCTTCAAAGCCATGTGGTCTCTGAATGGGCATGGCCTGAGCAAGGTGTTCACAG GGAGCAGGGCCCTGGAAGGAAAGGCCAAGGTGGGGAAGCTGAAGGATGGGGCTAGATCCATGTCTCGTAC CATCCAGTCCAACTTCTTCGACGGGGTGAAGCAGGAGGCCATCAAGCTACTGCTAGTTGGAGATGTCTAC AATGAAGAGTCTACAGACAAAGGCAGGATGCTGCTGGACAACACGGCCCTTCTGGGTTTGGGGTCAAATA AACAAAACAGTCTTTCAGGCATGCTGGATGGAAAAGCAACACCCAGGATCCTGAAGGCCATGACAGAACG CCAGTCAGAATTCACAAATTTCAAGCGGATCCAGATTGCTATGGGGACCTGGAATGTGAACGGAGGAAAA CAATTCCGTAGCAATCTCCTGGGGACCACTGAACTCACTGACTGGCTCCTGGATGCACCCCAGCTCTCAG GAGCAGTGGACTCTCAGGACGATGGCGGTCCCGCTGACATATTTGCCGTCGGGTTTGAGGAGATGGTGGA GCTGAGTGCAGGAAATATTGTCAATGCCAGCACCACCAACAGGAAGATGTGGGGTGAGCAGCTCCAGAAA GCCATCTCCCGATCCCATCGGTACATCCTCTTGACCTCAGCACAGCTGGTGGGCGTCTGTCTTTACATCT TTGTACGTCCGTACCACGTCCCGTTCATCAGGGATGTGGCCATCGACACCGTGAAGACCGGTATGGGGGG AAAGGCAGGGAATAAGGGCGCCGTGGGTATCCGCTTTCAGTTCCACAGCACTAGCTTCTGCTTCATCTGC AGTCACCTGACGGCTGGGCAGTCTCAGGTGAAGGAGAGGAACGAAGACTACCGGGAGATCACGCACAAGC TCTCCTTCCCTTCGGGGAGAAACATATTTTCACACGATTATGTGTTTTGGTGTGGCGATTTCAACTACCG CATCGATCTTACTTACGAAGAAGTCTTCTATTTTGTTAAACGCCAAGACTGGAAGAAACTTATGGAATTT GATCAGTTACAGCTACAGAAATCAAGTGGGAAAATTTTTAAAGACTTTCATGAAGGAACCATTAACTTCG GACCCACCTACAAGTATGACGTTGGATCGGCTGCCTATGACACAAGTGACAAGTGCCGGACCCCAGCCTG GACAGACAGGGTGCTGTGGTGGAGGAAGAAGCATCCGTACGATAAGACAGCTGGCGAACTCAACCTTCTA GACAGTGATCTAGACGGTGATGCCAACATCAGACATACCTGGTCTCCGGGCACTCTCAAGTACTACGGCC GTGCCGAGCTGCAGGCATCTGATCACAGACCTGTGCTGGCCATCGTAGAGGTGGAGGTTCAAGAGGTCGA TGTGGGAGCCCGGGAGAGAGTCTTCCAGGAAGTGTCCTCTGTCCAAGGCCCGCTGGACGCCACTGTTATT GTAAACCTTCAGTCTCCAACCCTTGAAGAGAGAAATGAATTTCCAGAGGACCTGCGCACAGAACTTATGC AGACTTTGGGGAATTATGGGACGATTATTCTGGTCAGGATCAACCAAGGGCAGATGCTGGTGACATTTGC AGACAGCCACTCAGCTCTCAGTGTGCTGGATGTGGATGGTATGAAGGTGAAAGGCAGGGCCGTGAAGATT CGACCAAAGACCAAAGATTGGCTAGAAGGCTTGAGAGAGGAGCTCATTCGGAAGCGGGACAGCATGGCCC CTGTGTCTCCCACCGCCAACTCCTGCTTGTTGGAGGAGAACTTTGACTTCACAAGTCTGGACTATGAGTC AGAAGGGGATGTTCTTGAAGATGATGAAGACTACTTAGCGGATGAGTTTGGTCAGCCTGTAGTCTCAGAC AGTGAGCTTGGAGGAGACGACTCTTCTGATACCATGAGCGCCTCGACACCTGCCAGCAAGTCTCCCGCAC TGGCTAAAAAGAAGCAACATCCAACGTACAAAGACGACGCTGACCTGATGACGTTAAAGCTGGAGCTGGA AGTTGCTGGGAATTTTCGCCACCGTTCTCCGAGCAGGTCCCTGTCTGTCCCCAATAGGCCTCGGCCACCT CACCCTCCACAAAGACCCCCCCCTCCAACTGGCTTAATGGTGAAGAAGTCTGCCTCAGATGCGTCTATCT CCTCCGGAACCCACGGGCAATATTCCATCTTGCAGACCGCGAAACTTCTTCCAGGAGCACCTCAGCAACC GCCCAAGGCTCGAACTGGAATAAGTAAACCTTACAACGTCAAACAGATCAAAACCACCAATGCTCAGGAG GCGGAAGCAGCTATCCGGTGTCTCCTGGAAGCTGGCGGAGGTGTCCCAGAGTCAGCCCCGGGTGCCACGC CCCTGAGAAACCAAGGGTCTTCTAAGCCAGAGGCCTCCCTGGGGCCTCCAGTCCTACCGCGCCGGCCTGT TCCAAGGGTTCCCACTATGAAGAAACCAACTTTGAGGAGAACAGGAAAGCCCATGTTATCAGAAGAACAG TGTGAACAGCAGCCTGTCCATTTTACAATGGCTTCCCAGGAAATGAACCTTGAGACCCCTCCTCCAATAA CAGCTCCCATCCCGCCTGTTCCCAAACCAAGAACATTTCAGCCTGGGAGAGGTGTTGAGAGGAGGCCGAG TGGTGGCAAGCCAGAACCGGATGACGCTCCTCCTGTGACAGGCGCTGTGGAGTTATCGTCTCCAGAGGCC CCAGAGGCCCCGTCCCTGGCTCCCAAGGTGCCTCCTAGGAGGAAGAAGTCTGCACCAGCAGCCTTCCACC TGCAGGTCCTGCAGAGCAACAGCCAACTTCTGCAGGGCCTCACATGCTCCAGCAGTTCTCCGTCCCCACC AAAGCCTGACACCCCCCTGCTCTATCCGCAAATGGCACTTGGCACTTCATCTGCTATAAGTCCCGAAACT GATGGCCCCAGAGTGACGGAGCCCGAGGCAGCCTCTTTTCATGGCGATTACCCAGATCCCTTCTGGAGCC TTCTCCACCACCCCAAGCTGTTGAATAATAATACCTGGCTGTCCAAGAGCTCTGAGCCTTTAGACTTGGG GTCCAGGACCCCTGAGAGGACACACACAGACTCAGCGCAGGTCAATGCATCAGTGGTTGAGAGGGGGCTT CCACCAGACCATGGGGGTAAAGACTTCAGTCACTGGATGGCCGCCAGTAACAAAGACAAGAGGACAACAT TAGGCGTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR200255 representing NM_001113371 Red=Cloning site Green=Tags(s) MALSKGLRLLARLDPTGPSSVLLEARGRGDCLLFEAGAVATLAPEEKEVIKGLYGKPTDAYGCLGELSLK SGGVPLSFLVLVTGCTSVGRIPDAEIYKITGTEFYPLQEEAKEEDRLPALKKILSSGVFYFAWPNDGACF DLTIRAQKQGDDCSEWGTSFFWNQLLHVPLRQHQVNCHDWLLKVICGVVTIRTVYASHKQAKACLISRIS CERAGARFLTRGVNDDGHVSNFVETEQAIYMDDGVSSFVQIRGSVPLFWEQPGLQVGSHHLRLHRGLEAN APAFERHMVLLKEQYGQQVVVNLLGSRGGEEVLNRAFKKLLWASCHAGDTPMINFDFHQFAKGRKLEKLE NLLRPQLKLHWDDFGVFAKGENVSPRFQKGTLRMNCLDCLDRTNTVQCFIALEVLHLQLESLGLNSKPIT DRFVESFKAMWSLNGHGLSKVFTGSRALEGKAKVGKLKDGARSMSRTIQSNFFDGVKQEAIKLLLVGDVY NEESTDKGRMLLDNTALLGLGSNKQNSLSGMLDGKATPRILKAMTERQSEFTNFKRIQIAMGTWNVNGGK QFRSNLLGTTELTDWLLDAPQLSGAVDSQDDGGPADIFAVGFEEMVELSAGNIVNASTTNRKMWGEQLQK AISRSHRYILLTSAQLVGVCLYIFVRPYHVPFIRDVAIDTVKTGMGGKAGNKGAVGIRFQFHSTSFCFIC SHLTAGQSQVKERNEDYREITHKLSFPSGRNIFSHDYVFWCGDFNYRIDLTYEEVFYFVKRQDWKKLMEF DQLQLQKSSGKIFKDFHEGTINFGPTYKYDVGSAAYDTSDKCRTPAWTDRVLWWRKKHPYDKTAGELNLL DSDLDGDANIRHTWSPGTLKYYGRAELQASDHRPVLAIVEVEVQEVDVGARERVFQEVSSVQGPLDATVI VNLQSPTLEERNEFPEDLRTELMQTLGNYGTIILVRINQGQMLVTFADSHSALSVLDVDGMKVKGRAVKI RPKTKDWLEGLREELIRKRDSMAPVSPTANSCLLEENFDFTSLDYESEGDVLEDDEDYLADEFGQPVVSD SELGGDDSSDTMSASTPASKSPALAKKKQHPTYKDDADLMTLKLELEVAGNFRHRSPSRSLSVPNRPRPP HPPQRPPPPTGLMVKKSASDASISSGTHGQYSILQTAKLLPGAPQQPPKARTGISKPYNVKQIKTTNAQE AEAAIRCLLEAGGGVPESAPGATPLRNQGSSKPEASLGPPVLPRRPVPRVPTMKKPTLRRTGKPMLSEEQ CEQQPVHFTMASQEMNLETPPPITAPIPPVPKPRTFQPGRGVERRPSGGKPEPDDAPPVTGAVELSSPEA PEAPSLAPKVPPRRKKSAPAAFHLQVLQSNSQLLQGLTCSSSSPSPPKPDTPLLYPQMALGTSSAISPET DGPRVTEPEAASFHGDYPDPFWSLLHHPKLLNNNTWLSKSSEPLDLGSRTPERTHTDSAQVNASVVERGL PPDHGGKDFSHWMAASNKDKRTTLGV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001113371
ORF Size	4488 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001113371.1, NP_001106842.1
RefSeq Size	4985 bp
RefSeq ORF	4491 bp
Locus ID	84018
UniProt ID	O55207
MW	165.3 kDa
Gene Summary	Inositol 5-phosphatase; may be involved in membrane trafficking and signal transduction pathways [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.