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TBC1D4 (NM_001286659) Human Tagged ORF Clone
CAT＃: RG240068
- TrueORF®
TBC1D4 (tGFP-tagged) - Human TBC1 domain family, member 4 (TBC1D4), transcript variant 3
ORF Plasmid: DDK
"NM_001286659" in other vectors (2)
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CNY 12,730.00
货期*
2周
规格
Product images
经常一起买 (4)
TBC1D4 mouse monoclonal antibody, clone OTI5E6 (formerly 5E6)
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Tag | TurboGFP |
| Synonyms | AS160; NIDDM5 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG240068 representing NM_001286659.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGCCGCCCAGCTGCATTCAGGATGAGCCGTTCCCGCACCCCCTGGAGCCCGAGCCGGGCGTCTCA GCTCAGCCCGGCCCCGGGAAGCCAAGCGATAAGCGGTTCCGGCTGTGGTACGTTGGGGGGTCGTGCCTG GACCACAGGACCACGCTGCCTATGCTGCCCTGGCTCATGGCCGAGATCCGCAGGCGCAGCCAGAAGCCC GAGGCGGGCGGCTGCGGGGCGCCGGCGGCCCGAGAGGTGATCCTGGTGCTCAGCGCGCCCTTCCTGCGT TGCGTCCCCGCGCCGGGCGCTGGGGCCTCGGGGGGCACTAGTCCGTCGGCCACGCAGCCCAACCCGGCG GTATTCATCTTCGAGCACAAGGCGCAGCATATCTCGCGCTTCATCCACAACAGCCACGACCTCACCTAC TTTGCCTACCTGATCAAGGCGCAGCCCGACGACCCCGAGTCGCAGATGGCCTGCCACGTTTTCCGCGCC ACAGACCCCAGCCAGGTTCCTGATGTTATTAGCAGCATAAGGCAATTATCTAAAGCGGCCATGAAAGAG GATGCCAAACCCAGCAAAGATAATGAGGACGCCTTTTACAACTCTCAGAAGTTCGAAGTCCTGTACTGT GGAAAGGTGACCGTGACCCACAAGAAGGCCCCCTCAAGCCTCATCGATGACTGCATGGAGAAGTTCAGC CTGCACGAACAGCAGCGCCTGAAGATCCAAGGGGAGCAGCGCGGTCCGGACCCAGGAGAGGACCTGGCT GACTTGGAGGTGGTGGTGCCCGGGTCCCCCGGAGACTGCCTGCCGGAGGAGGCTGACGGCACCGACACC CACCTTGGCTTACCTGCCGGGGCCAGCCAGCCTGCCCTGACCAGCTCTCGGGTCTGCTTCCCTGAGCGG ATTTTGGAAGATTCTGGCTTTGATGAGCAGCAGGAGTTTCGGTCTCGGTGCAGCAGTGTCACCGGCGTG CAACGGAGAGTTCACGAGGGCAGCCAGAAATCCCAGCCGCGACGGAGACACGCGAGCGCACCCAGTCAC GTCCAGCCCTCGGACTCGGAGAAGAACAGGACCATGCTCTTCCAGGTTGGGCGATTTGAGATTAACCTT ATCAGTCCAGACACTAAATCAGTTGTGCTAGAAAAGAATTTTAAAGATATCTCCTCTTGTTCTCAGGGT ATAAAGCATGTGGATCACTTTGGCTTTATCTGCCGGGAGTCTCCAGAGCCTGGACTTAGCCAGTATATT TGTTATGTATTCCAGTGTGCCAGCGAATCTCTGGTTGATGAGGTAATGCTGACTCTGAAACAGGCCTTC AGTACGGCGGCTGCCCTGCAGAGTGCCAAGACGCAGATTAAACTGTGTGAGGCCTGCCCGATGCACTCT TTGCATAAGCTCTGTGAAAGGATTGAAGGTCTCTACCCACCAAGAGCCAAGCTGGTGATACAGAGGCAT CTCTCATCACTGACAGATAATGAGCAAGCTGACATCTTTGAAAGAGTTCAGAAAATGAAGCCAGTCAGT GACCAGGAAGAAAATGAACTTGTGATTTTACACCTGAGGCAGCTGTGTGAAGCCAAGCAGAAGACACAC GTGCACATCGGGGAAGGCCCTTCTACTATTTCAAATAGTACAATCCCAGAAAATGCAACAAGCAGTGGA AGGTTCAAACTTGACATTCTGAAAAATAAAGCTAAGAGATCCTTAACTAGCTCCCTGGAAAATATCTTC TCAAGGGGAGCTAACAGAATGAGAGGTCGGCTTGGAAGTGTGGACAGTTTTGAACGGTCCAACAGTCTT GCTTCAGAGAAGGACTACTCACCAGGGGATTCTCCACCAGGGACACCGCCAGCGTCCCCACCGTCCTCA GCTTGGCAAACGTTTCCCGAAGAGGATTCCGACTCCCCGCAGTTTCGAAGACGGGCACACACGTTCAGC CACCCACCTTCAAGCACAAAGAGAAAGCTGAATTTGCAGGATGGGAGGGCTCAGGGTGTGCGTTCCCCT CTGCTGAGGCAGAGCTCCAGTGAACAGTGCAGTGATGGAGAAGGGAGAAAAAGGACCTCATCTACCTGC AGCAATGAGTCCCTAAGTGTGGGAGGAACCTCTGTCACTCCTCGCCGGATCTCCTGGCGGCAGCGCATT TTCCTCAGGGTTGCTTCTCCCATGAACAAATCTCCCTCAGCAATGCAACAGCAAGATGGATTGGACAGG AACGAGCTGCTGCCACTGTCCCCCCTCTCTCCAACCATGGAGGAGGAACCGCTGGTTGTATTCCTGTCT GGGGAGGATGACCCAGAAAAGATTGAAGAAAGAAAGAAATCAAAAGAACTGAGGAGCTTGTGGAGAAAA GCTATACACCAACAAATCTTGTTACTTCGAATGGAAAAAGAAAACCAGAAACTTGAAGCAAGCAGAGAT GAACTCCAGTCCAGAAAAGTTAAATTAGACTATGAAGAAGTTGGTGCATGTCAGAAAGAGGTCTTAATA ACTTGGGATAAGAAGTTGTTAAACTGCAGAGCTAAAATCAGATGTGATATGGAAGATATTCATACTCTT CTTAAAGAAGGAGTTCCCAAAAGTCGACGAGGAGAAATTTGGCAGTTTCTGGCTTTACAGTACCGACTC AGACACAGATTGCCTAATAAACAACAGCCTCCTGACATATCCTATAAGGAACTTTTGAAGCAGCTCACT GCTCAGCAGCATGCGATTCTCGTGGATTTAGGAAGGACGTTTCCTACTCACCCTTACTTTTCAGTACAG CTTGGGCCAGGACAGCTGTCACTGTTTAACCTCCTGAAAGCCTATTCTTTGCTGGACAAAGAAGTGGGA TACTGTCAGGGGATCAGCTTTGTGGCTGGAGTCCTGCTTCTGCACATGAGTGAAGAGCAAGCCTTTGAA ATGCTGAAATTCCTCATGTATGACCTCGGCTTCCGCAAGCAGTACAGACCTGACATGATGTCGCTGCAG ATTCAAATGTACCAGCTGTCCAGGCTCCTTCATGACTATCACAGAGATCTCTACAATCACCTTGAAGAA AATGAAATCAGCCCCAGTCTTTATGCTGCCCCCTGGTTCCTCACATTGTTTGCCTCTCAGTTTTCATTA GGATTTGTAGCCAGAGTTTTTGATATTATTTTTCTTCAGGGAACTGAAGTTATATTCAAGGTTGCACTC AGCCTACTGAGCAGCCAAGAGACACTTATAATGGAATGTGAGAGCTTTGAAAATATTGTTGAGTTTCTT AAAAACACGCTACCTGATATGAATACCTCTGAAATGGAAAAAATTATTACCCAGGTTTTTGAGATGGAT ATTTCTAAGCAGTTGCATGCCTATGAGGTGGAATATCATGTGCTACAGGATGAGCTTCAGGAATCTTCA TATTCCTGTGAGGATAGTGAAACTTTGGAGAAGCTGGAGAGGGCCAATAGCCAACTGAAAAGACAAAAC ATGGACCTCCTAGAAAAATTACAGGTAGCTCATACTAAAATCCAGGCCTTGGAATCAAACCTGGAAAAT CTTTTGACGAGAGAGACCAAAATGAAGTCTTTAATCCGGACCCTGGAACAAGAAAAAATGGCTTATCAA AAGACAGTGGAGCAACTCCGGAAGCTGCTGCCCGCGGATGCTCTAGTCAATTGTGACCTGTTGCTGAGA GACCTAAACTGCAACCCTAACAACAAAGCCAAGATAGGAAATAAGCCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG240068
Blue=ORF Red=Cloning site Green=Tag(s) MEPPSCIQDEPFPHPLEPEPGVSAQPGPGKPSDKRFRLWYVGGSCLDHRTTLPMLPWLMAEIRRRSQKP EAGGCGAPAAREVILVLSAPFLRCVPAPGAGASGGTSPSATQPNPAVFIFEHKAQHISRFIHNSHDLTY FAYLIKAQPDDPESQMACHVFRATDPSQVPDVISSIRQLSKAAMKEDAKPSKDNEDAFYNSQKFEVLYC GKVTVTHKKAPSSLIDDCMEKFSLHEQQRLKIQGEQRGPDPGEDLADLEVVVPGSPGDCLPEEADGTDT HLGLPAGASQPALTSSRVCFPERILEDSGFDEQQEFRSRCSSVTGVQRRVHEGSQKSQPRRRHASAPSH VQPSDSEKNRTMLFQVGRFEINLISPDTKSVVLEKNFKDISSCSQGIKHVDHFGFICRESPEPGLSQYI CYVFQCASESLVDEVMLTLKQAFSTAAALQSAKTQIKLCEACPMHSLHKLCERIEGLYPPRAKLVIQRH LSSLTDNEQADIFERVQKMKPVSDQEENELVILHLRQLCEAKQKTHVHIGEGPSTISNSTIPENATSSG RFKLDILKNKAKRSLTSSLENIFSRGANRMRGRLGSVDSFERSNSLASEKDYSPGDSPPGTPPASPPSS AWQTFPEEDSDSPQFRRRAHTFSHPPSSTKRKLNLQDGRAQGVRSPLLRQSSSEQCSDGEGRKRTSSTC SNESLSVGGTSVTPRRISWRQRIFLRVASPMNKSPSAMQQQDGLDRNELLPLSPLSPTMEEEPLVVFLS GEDDPEKIEERKKSKELRSLWRKAIHQQILLLRMEKENQKLEASRDELQSRKVKLDYEEVGACQKEVLI TWDKKLLNCRAKIRCDMEDIHTLLKEGVPKSRRGEIWQFLALQYRLRHRLPNKQQPPDISYKELLKQLT AQQHAILVDLGRTFPTHPYFSVQLGPGQLSLFNLLKAYSLLDKEVGYCQGISFVAGVLLLHMSEEQAFE MLKFLMYDLGFRKQYRPDMMSLQIQMYQLSRLLHDYHRDLYNHLEENEISPSLYAAPWFLTLFASQFSL GFVARVFDIIFLQGTEVIFKVALSLLSSQETLIMECESFENIVEFLKNTLPDMNTSEMEKIITQVFEMD ISKQLHAYEVEYHVLQDELQESSYSCEDSETLEKLERANSQLKRQNMDLLEKLQVAHTKIQALESNLEN LLTRETKMKSLIRTLEQEKMAYQKTVEQLRKLLPADALVNCDLLLRDLNCNPNNKAKIGNKP TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001286659 |
| ORF Size | 3705 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reference Data | |
| RefSeq | NM_001286659.2 |
| RefSeq Size | 6200 bp |
| RefSeq ORF | 3708 bp |
| Locus ID | 9882 |
| UniProt ID | O60343 |
| MW | 140 kDa |
| Gene Summary | This gene is a member of the Tre-2/BUB2/CDC16 domain family. The protein encoded by this gene is a Rab-GTPase-activating protein, and contains two phopshotyrosine-binding domains (PTB1 and PTB2), a calmodulin-binding domain (CBD), a Rab-GTPase domain, and multiple AKT phosphomotifs. This protein is thought to play an important role in glucose homeostasis by regulating the insulin-dependent trafficking of the glucose transporter 4 (GLUT4), important for removing glucose from the bloodstream into skeletal muscle and fat tissues. Reduced expression of this gene results in an increase in GLUT4 levels at the plasma membrane, suggesting that this protein is important in intracellular retention of GLUT4 under basal conditions. When exposed to insulin, this protein is phosphorylated, dissociates from GLUT4 vesicles, resulting in increased GLUT4 at the cell surface, and enhanced glucose transport. Phosphorylation of this protein by AKT is required for proper translocation of GLUT4 to the cell surface. Individuals homozygous for a mutation in this gene are at higher risk for type 2 diabetes and have higher levels of circulating glucose and insulin levels after glucose ingestion. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015] |
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