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TBC1D1 (NM_001253912) Human Tagged ORF Clone
CAT＃: RG235198
- TrueORF®
TBC1D1 (tGFP-tagged) - Homo sapiens TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1 (TBC1D1), transcript variant 2
ORF Plasmid: DDK
"NM_001253912" in other vectors (2)
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CNY 12,730.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | TBC; TBC1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG235198 representing NM_001253912
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACCAATAACATTCACAGCAAGGAAACATCTGCTTTCTAACGAGGTCTCGGTGGATTTTGGCCTGC AGCTGGTGGGCTCCCTGCCTGTGCATTCCCTGACCACCATGCCCATGCTGCCCTGGGTTGTGGCTGAGGT GCGAAGACTCAGCAGGCAGTCCACCAGAAAGGAACCTGTAACCAAGCAAGTCCGGCTTTGCGTTTCACCC TCTGGACTGAGATGTGAACCTGAGCCAGGGAGAAGTCAACAGTGGGATCCCCTGATCTATTCCAGCATCT TTGAGTGCAAGCCTCAGCGTGTTCACAAACTGATTCACAACAGTCATGACCCAAGTTACTTTGCTTGTCT GATTAAGGAAGACGCTGTCCACCGGCAGAGTATCTGCTATGTGTTCAAAGCCGATGATCAAACAAAAGTG CCTGAGATCATCAGCTCCATCCGTCAGGCGGGGAAGATCGCCCGGCAGGAGGAGCTGCACTGCCCGTCCG AGTTCGACGACACGTTTTCCAAGAAGTTCGAGGTGCTCTTCTGCGGCCGCGTGACGGTGGCGCACAAGAA GGCTCCGCCGGCCCTGATCGACGAGTGCATCGAGAAGTTCAATCACGTCAGCGGCAGCCGGGGGTCCGAG AGCCCCCGCCCCAACCCGCCCCATGCCGCGCCCACAGGGAGCCAGGAGCCTGTGCGCAGGCCCATGCGCA AGTCCTTCTCCCAGCCCGGCCTGCGCTCGCTGGCCTTTAGGAAGGAGCTGCAGGATGGGGGCCTCCGAAG CAGCGGCTTCTTCAGCTCCTTCGAGGAGAGCGACATTGAGAACCACCTCATTAGCGGACACAATATTGTG CAGCCCACAGATATCGAGGAAAATCGAACTATGCTCTTCACGATTGGCCAGTCTGAAGTTTACCTCATCA GTCCTGACACCAAAAAAATAGCATTGGAGAAAAATTTTAAGGAGATATCCTTTTGCTCTCAGGGCATCAG ACACGTGGACCACTTTGGGTTTATCTGTCGGGAGTCTTCCGGAGGTGGCGGCTTTCATTTTGTCTGTTAC GTGTTTCAGTGCACAAATGAGGCTCTGGTTGATGAAATTATGATGACCCTGAAACAGGCCTTCACGGTGG CCGCAGTGCAGCAGACAGCTAAGGCGCCAGCCCAGCTGTGTGAGGGCTGCCCCCTGCAAAGCCTGCACAA GCTCTGTGAGAGGATAGAGGGAATGAATTCTTCCAAAACAAAACTAGAACTGCAAAAGCACCTGACGACA TTAACCAATCAGGAGCAGGCGACTATTTTTGAAGAGGTTCAGAAATTGAGACCGAGAAATGAGCAGCGAG AGAATGAATTGATTATTTCTTTTCTGAGATGTTTATATGAAGAGAAACAGAAAGAACACATCCATATTGG GGAGATGAAGCAGACATCGCAGATGGCAGCAGAGAATATTGGAAGTGAATTACCACCCAGTGCCACTCGA TTTAGGCTAGATATGCTGAAAAACAAAGCAAAGAGATCTTTAACAGAGTCTTTAGAAAGTATTTTGTCCC GGGGTAATAAAGCCAGAGGCCTGCAGGAACACTCCATCAGTGTGGATCTGGATAGCTCCCTGTCTAGTAC ATTAAGTAACACCAGCAAAGAGCCATCTGTGTGTGAAAAGGAGGCCTTGCCCATCTCTGAGAGCTCCTTT AAGCTCCTCGGCTCCTCGGAGGACCTGTCCAGTGACTCGGAGAGTCATCTCCCAGAAGAGCCAGCTCCGC TGTCGCCCCAGCAGGCCTTCAGGAGGCGAGCAAACACCCTGAGTCACTTCCCCATCGAATGCCAGGAACC TCCACAACCTGCCCGGGGGTCCCCGGGGGTTTCGCAAAGGAAACTTATGAGGTATCACTCAGTGAGCACA GAGACGCCTCATGAACGAAATGTGGATCCTTCACCTGTGGGTGAGTCTAAGCACCGCCCAGGTCAGTCTT CAGCTCCTGCTCCTCCACCTCGTCTTAACCCCTCCGCCTCCTCGCCAAACTTTTTTAAGTACCTAAAACA TAATTCCAGTGGAGAACAAAGTGGGAATGCTGTGCCAAAGAGCATCTCCTACCGTAATGCCCTGCGGAAA AAACTTCATTCTTCTTCCTCTGTGCCAAATTTTCTAAAATTTCTGGCTCCTGTAGATGAAAATAACACCT CTGATTTTATGAACACAAAAAGGGACTTTGAATCCAAAGCAAACCATCTTGGTGATTCTGGTGGGACTCC TGTGAAGACCCGGAGGCATTCCTGGAGGCAGCAGATATTCCTCCGAGTAGCCACCCCGCAGAAGGCGTGC GATTCTTCCAGCAGATATGAAGATTATTCAGAGCTGGGAGAGCTTCCCCCACGATCTCCTTTAGAACCAG TTTGTGAAGATGGGCCCTTTGGCCCCCCACCAGAGGAAAAGAAAAGGACATCTCGTGAGCTCCGAGAGCT GTGGCAAAAGGCTATTCTTCAACAGATACTGCTGCTTAGAATGGAGAAGGAAAATCAGAAGCTCCAAGCC TCTGAAAATGATTTGCTGAACAAGCGCCTGAAGCTCGATTATGAAGAAATTACTCCCTGTCTTAAAGAAG TAACTACAGTGTGGGAAAAGATGCTTAGCACTCCAGGAAGATCAAAAATTAAGTTTGACATGGAAAAAAT GCACTCGGCTGTTGGGCAAGGTGTGCCACGTCATCACCGAGGTGAAATCTGGAAATTTCTAGCTGAGCAA TTCCACCTTAAACACCAGTTTCCCAGCAAACAGCAGCCAAAGGATGTGCCATACAAAGAACTCTTAAAGC AGCTGACTTCCCAGCAGCATGCGATTCTTATTGACCTTGGGCGAACCTTTCCTACACACCCATACTTCTC TGCCCAGCTTGGAGCAGGACAGCTATCGCTTTACAACATTTTGAAGGCCTACTCACTTCTAGACCAGGAA GTGGGATATTGCCAAGGTCTCAGCTTTGTAGCAGGCATTTTGCTTCTTCATATGAGTGAGGAAGAGGCGT TTAAAATGCTCAAGTTTCTGATGTTTGACATGGGGCTGCGGAAACAGTATCGGCCAGACATGATTATTTT ACAGATGGAAAAGACCATCAATCAGGTATTTGAAATGGACATCGCTAAACAGTTACAAGCTTATGAAGTT GAGTACCACGTCCTTCAAGAAGAACTTATCGATTCCTCTCCTCTCAGTGACAACCAAAGAATGGATAAAT TAGAGAAAACCAACAGCAGCTTACGCAAACAGAACCTTGACCTCCTTGAACAGTTGCAGGTGGCAAATGG TAGGATCCAAAGCCTTGAGGCCACCATTGAGAAGCTCCTGAGCAGTGAGAGCAAGCTGAAGCAGGCCATG CTTACCTTAGAACTGGAGCGGTCGGCCCTGCTGCAGACGGTGGAGGAGCTGCGGCGGCGGAGCGCAGAGC CCAGCGACCGGGAGCCTGAGTGCACGCAGCCCGAGCCCACGGGCGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG235198 representing NM_001253912
Red=Cloning site Green=Tags(s) MEPITFTARKHLLSNEVSVDFGLQLVGSLPVHSLTTMPMLPWVVAEVRRLSRQSTRKEPVTKQVRLCVSP SGLRCEPEPGRSQQWDPLIYSSIFECKPQRVHKLIHNSHDPSYFACLIKEDAVHRQSICYVFKADDQTKV PEIISSIRQAGKIARQEELHCPSEFDDTFSKKFEVLFCGRVTVAHKKAPPALIDECIEKFNHVSGSRGSE SPRPNPPHAAPTGSQEPVRRPMRKSFSQPGLRSLAFRKELQDGGLRSSGFFSSFEESDIENHLISGHNIV QPTDIEENRTMLFTIGQSEVYLISPDTKKIALEKNFKEISFCSQGIRHVDHFGFICRESSGGGGFHFVCY VFQCTNEALVDEIMMTLKQAFTVAAVQQTAKAPAQLCEGCPLQSLHKLCERIEGMNSSKTKLELQKHLTT LTNQEQATIFEEVQKLRPRNEQRENELIISFLRCLYEEKQKEHIHIGEMKQTSQMAAENIGSELPPSATR FRLDMLKNKAKRSLTESLESILSRGNKARGLQEHSISVDLDSSLSSTLSNTSKEPSVCEKEALPISESSF KLLGSSEDLSSDSESHLPEEPAPLSPQQAFRRRANTLSHFPIECQEPPQPARGSPGVSQRKLMRYHSVST ETPHERNVDPSPVGESKHRPGQSSAPAPPPRLNPSASSPNFFKYLKHNSSGEQSGNAVPKSISYRNALRK KLHSSSSVPNFLKFLAPVDENNTSDFMNTKRDFESKANHLGDSGGTPVKTRRHSWRQQIFLRVATPQKAC DSSSRYEDYSELGELPPRSPLEPVCEDGPFGPPPEEKKRTSRELRELWQKAILQQILLLRMEKENQKLQA SENDLLNKRLKLDYEEITPCLKEVTTVWEKMLSTPGRSKIKFDMEKMHSAVGQGVPRHHRGEIWKFLAEQ FHLKHQFPSKQQPKDVPYKELLKQLTSQQHAILIDLGRTFPTHPYFSAQLGAGQLSLYNILKAYSLLDQE VGYCQGLSFVAGILLLHMSEEEAFKMLKFLMFDMGLRKQYRPDMIILQMEKTINQVFEMDIAKQLQAYEV EYHVLQEELIDSSPLSDNQRMDKLEKTNSSLRKQNLDLLEQLQVANGRIQSLEATIEKLLSSESKLKQAM LTLELERSALLQTVEELRRRSAEPSDREPECTQPEPTGD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001253912 |
| ORF Size | 3477 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001253912.2 |
| RefSeq Size | 5678 bp |
| RefSeq ORF | 3480 bp |
| Locus ID | 23216 |
| UniProt ID | Q86TI0 |
| Protein Families | Druggable Genome |
| Gene Summary | TBC1D1 is the founding member of a family of proteins sharing a 180- to 200-amino acid TBC domain presumed to have a role in regulating cell growth and differentiation. These proteins share significant homology with TRE2 (USP6; MIM 604334), yeast Bub2, and CDC16 (MIM 603461) (White et al., 2000 [PubMed 10965142]).[supplied by OMIM, Mar 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC235198 | TBC1D1 (Myc-DDK tagged) - Homo sapiens TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1 (TBC1D1), transcript variant 2 |
CNY 11,590.00 |
|
| SC332253 | TBC1D1 (untagged) - Homo sapiens TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1 (TBC1D1), transcript variant 2 |
CNY 11,120.00 |
