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Metabotropic Glutamate Receptor 4 (GRM4) (NM_001256811) Human Tagged ORF Clone
CAT＃: RG234974
- TrueORF®
GRM4 (tGFP-tagged) - Homo sapiens glutamate receptor, metabotropic 4 (GRM4), transcript variant 4
ORF Plasmid: DDK
"NM_001256811" in other vectors (2)
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CNY 7,890.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | GPRC1D; mGlu4; MGLUR4 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG234974 representing NM_001256811
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTGGGAAGAGAGGCTTGGGCTGGTGGTGGGCCCGGCTGCCCCTTTGCCTGCTCCTCAGCCTTTACG GCCCCTGGATGCCTTCCTCCCTGGGAAAGCCCAAAGGCCACCCTCACATGAATTCCATCCGCATAGATGG GGACATCACACTGGGAGGCCTGTTCCCGGTGCATGGCCGGGGCTCAGAGGGCAAGCCCTGTGGAGAACTT AAGAAGGAAAAGGGCATCCACCGGCTGGAGGCCATGCTGTTCGCCCTGGATCGCATCAACAACGACCCGG ACCTGCTGCCTAACATCACGCTGGGCGCCCGCATTCTGGACACCTGCTCCAGGGACACCCATGCCCTCGA GCAGTCGCTGACCTTTGTGCAGGCGCTCATCGAGAAGGATGGCACAGAGGTCCGCTGTGGCAGTGGCGGC CCACCCATCATCACCAAGCCTGAACGTGTGGTGGGTGTCATCGGTGCTTCAGGGAGCTCGGTCTCCATCA TGGTGGCCAACATCCTTCGCCTCTTCAAGATACCCCAGATCAGCTACGCCTCCACAGCGCCAGACCTGAG TGACAACAGCCGCTACGACTTCTTCTCCCGCGTGGTGCCCTCGGACACGTACCAGGCCCAGGCCATGGTG GACATCGTCCGTGCCCTCAAGTGGAACTATGTGTCCACAGTGGCCTCGGAGGGCAGCTATGGTGAGAGCG GTGTGGAGGCCTTCATCCAGAAGTCCCGTGAGGACGGGGGCGTGTGCATCGCCCAGTCGGTGAAGATACC ACGGGAGCCCAAGGCAGGCGAGTTCGACAAGATCATCCGCCGCCTCCTGGAGACTTCGAACGCCAGGGCA GTCATCATCTTTGCCAACGAGGATGACATCAGGCGTGTGCTGGAGGCAGCACGAAGGGCCAACCAGACAG GCCATTTCTTCTGGATGGGCTCTGACAGCTGGGGCTCCAAGATTGCACCTGTGCTGCACCTGGAGGAGGT GGCTGAGGGTGCTGTCACGATCCTCCCCAAGAGGATGTCCGTACGAGACCGTGAGCGAATTGGGCAGGAT TCAGCTTATGAGCAGGAGGGGAAGGTGCAGTTTGTGATCGATGCCGTGTACGCCATGGGCCACGCGCTGC ACGCCATGCACCGTGACCTGTGTCCCGGCCGCGTGGGGCTCTGCCCGCGCATGGACCCTGTAGATGGCAC CCAGCTGCTTAAGTACATCCGAAACGTCAACTTCTCAGGCATCGCAGGGAACCCTGTGACCTTCAATGAG AATGGAGATGCGCCTGGGCGCTATGACATCTACCAATACCAGCTGCGCAACGATTCTGCCGAGTACAAGG TCATTGGCTCCTGGACTGACCACCTGCACCTTAGAATAGAGCGGATGCACTGGCCGGGGAGCGGGCAGCA GCTGCCCCGCTCCATCTGCAGCCTGCCCTGCCAACCGGGTGAGCGGAAGAAGACAGTGAAGGGCATGCCT TGCTGCTGGCACTGCGAGCCTTGCACAGGGTACCAGTACCAGGTGGACCGCTACACCTGTAAGACGTGTC CCTATGACATGCGGCCCACAGAGAACCGCACGGGCTGCCGGCCCATCCCCATCATCAAGCTTGAGTGGGG CTCGCCCTGGGCCGTGCTGCCCCTCTTCCTGGCCGTGGTGGGCATCGCTGCCACGTTGTTCGTGGTGATC ACCTTTGTGCGCTACAACGACACGCCCATCGTCAAGGCCTCGGGCCGTGAACTGAGCTACGTGCTGCTGG CAGGCATCTTCCTGTGCTATGCCACCACCTTCCTCATGATCGCTGAGCCCGACCTTGGCACCTGCTCGCT GCGCCGAATCTTCCTGGGACTAGGGATGAGCATCAGCTATGCAGCCCTGCTCACCAAGACCAACCGCATC TACCGCATCTTCGAGCAGGGCAAGCGCTCGGTCAGTGCCCCACGCTTCATCAGCCCCGCCTCACAGCTGG CCATCACCTTCAGCCTCATCTCGCTGCAGCTGCTGGGCATCTGTGTGTGGTTTGTGGTGGACCCCTCCCA CTCGGTGGTGGACTTCCAGGACCAGCGGACACTCGACCCCCGCTTCGCCAGGGGTGTGCTCAAGTGTGAC ATCTCGGACCTGTCGCTCATCTGCCTGCTGGGCTACAGCATGCTGCTCATGGTCACGTGCACCGTGTATG CCATCAAGACACGCGGCGTGCCCGAGACCTTCAATGAGGCCAAGCCCATTGGCTTCACCATGTACACCAC TTGCATCGTCTGGCTGGCCTTCATCCCCATCTTCTTTGGCACCTCGCAGTCGGCCGACAAGCTGTACATC CAGACGACGACGCTGACGGTCTCGGTGAGTCTGAGCGCCTCGGTGTCCCTGGGAATGCTCTACATGCCCA AAGTCTACATCATCCTCTTCCACCCGGAGCAGAACGTGCCCAAGCGCAAGCGCAGCCTCAAAGCCGTCGT TACGGCGGCCACCATGTCCAACAAGTTCACGCAGAAGGGCAACTTCCGGCCCAACGGAGAGGCCAAGTCT GAGCTCTGCGAGAACCTTGAGGCCCCAGCGCTGGCCACCAAACAGACTTACGTCACTTACACCAACCATG CAATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234974 representing NM_001256811
Red=Cloning site Green=Tags(s) MPGKRGLGWWWARLPLCLLLSLYGPWMPSSLGKPKGHPHMNSIRIDGDITLGGLFPVHGRGSEGKPCGEL KKEKGIHRLEAMLFALDRINNDPDLLPNITLGARILDTCSRDTHALEQSLTFVQALIEKDGTEVRCGSGG PPIITKPERVVGVIGASGSSVSIMVANILRLFKIPQISYASTAPDLSDNSRYDFFSRVVPSDTYQAQAMV DIVRALKWNYVSTVASEGSYGESGVEAFIQKSREDGGVCIAQSVKIPREPKAGEFDKIIRRLLETSNARA VIIFANEDDIRRVLEAARRANQTGHFFWMGSDSWGSKIAPVLHLEEVAEGAVTILPKRMSVRDRERIGQD SAYEQEGKVQFVIDAVYAMGHALHAMHRDLCPGRVGLCPRMDPVDGTQLLKYIRNVNFSGIAGNPVTFNE NGDAPGRYDIYQYQLRNDSAEYKVIGSWTDHLHLRIERMHWPGSGQQLPRSICSLPCQPGERKKTVKGMP CCWHCEPCTGYQYQVDRYTCKTCPYDMRPTENRTGCRPIPIIKLEWGSPWAVLPLFLAVVGIAATLFVVI TFVRYNDTPIVKASGRELSYVLLAGIFLCYATTFLMIAEPDLGTCSLRRIFLGLGMSISYAALLTKTNRI YRIFEQGKRSVSAPRFISPASQLAITFSLISLQLLGICVWFVVDPSHSVVDFQDQRTLDPRFARGVLKCD ISDLSLICLLGYSMLLMVTCTVYAIKTRGVPETFNEAKPIGFTMYTTCIVWLAFIPIFFGTSQSADKLYI QTTTLTVSVSLSASVSLGMLYMPKVYIILFHPEQNVPKRKRSLKAVVTAATMSNKFTQKGNFRPNGEAKS ELCENLEAPALATKQTYVTYTNHAI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001256811 |
| ORF Size | 2595 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001256811.2, NP_001243740.1 |
| RefSeq Size | 7293 bp |
| RefSeq ORF | 2598 bp |
| Locus ID | 2914 |
| UniProt ID | Q14833 |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| Protein Pathways | Neuroactive ligand-receptor interaction, Taste transduction |
| Gene Summary | L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012] |
Documents
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Resources
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