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CNOT2 (NM_001199303) Human Tagged ORF Clone
CAT＃: RG232951
- TrueORF®
CNOT2 (tGFP-tagged) - Homo sapiens CCR4-NOT transcription complex, subunit 2 (CNOT2), transcript variant 1
ORF Plasmid: DDK
"NM_001199303" in other vectors (2)
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CNY 4,370.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CDC36; HSPC131; IDNADFS; NOT2; NOT2H |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG232951 representing NM_001199303
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGAGGACTGATGGACATACATTATCTGAGAAAAGAAACTACCAGGTGACAAACAGCATGTTTGGTG CTTCAAGAAAGAAGTTTGTAGAGGGGGTCGACAGTGACTACCATGACGAAAACATGTACTACAGCCAGTC TTCTATGTTTCCACATCGGTCAGAAAAAGATATGCTGGCATCACCATCTACATCAGGTCAGCTGTCTCAG TTTGGGGCAAGTTTATACGGGCAACAAAGTGCACTAGGCCTTCCAATGAGGGGGATGAGCAACAATACCC CTCAGTTAAATCGCAGCTTATCACAAGGCACTCAGTTACCGAGCCACGTCACGCCAACAACAGGGGTACC AACAATGTCACTTCACACGCCTCCATCTCCAAGCAGGGGTATTTTGCCTATGAATCCTAGGAATATGATG AACCACTCCCAGGTTGGTCAGGGCATTGGAATTCCTAGCAGGACAAATAGCATGAGCAGTTCAGGGTTAG GTAGCCCCAACAGAAGCTCGCCAAGCATAATATGTATGCCAAAGCAGCAGCCTTCTCGACAGCCTTTTAC TGTGAACAGTATGTCTGGATTTGGAATGAACAGGAATCAGGCATTTGGAATGAATAACTCCTTATCAAGT AACATTTTTAATGGAACAGACGGAAGTGAAAATGTGACAGGATTGGACCTTTCAGATTTCCCAGCATTAG CAGACCGAAACAGGAGGGAAGGAAGTGGTAACCCAACTCCATTAATAAACCCCTTGGCTGGAAGAGCTCC TTATGTTGGAATGGTAACAAAACCAGCAAATGAACAATCCCAGGACTTCTCAATACACAATGAAGATTTT CCAGCATTACCAGGCTCCAGCTATAAAGATCCAACATCAAGTAATGATGACAGTAAATCTAATTTGAATA CATCTGGCAAGACAACTTCAAGTACAGATGGACCCAAATTCCCTGGAGATAAAAGTTCAACAACACAAAA TAATAACCAGCAGAAAAAAGGGATCCAGGTGTTACCTGATGGTCGGGTTACTAACATTCCTCAAGGGATG GTGACGGACCAATTTGGAATGATTGGCCTGTTAACATTTATCAGGGCAGCAGAGACAGACCCAGGAATGG TACATCTTGCATTAGGAAGTGACTTAACAACATTAGGCCTCAATCTGAACTCTCCTGAAAATCTCTACCC CAAATTTGCGTCACCCTGGGCATCTTCACCTTGTCGACCTCAAGACATAGACTTCCATGTTCCATCTGAG TACTTAACGAACATTCACATTAGGGATAAGCTGGCTGCAATAAAACTTGGCCGATATGGTGAAGACCTTC TCTTCTATCTCTATTACATGAATGGAGGAGACGTATTACAACTTTTAGCTGCAGTGGAGCTTTTTAACCG TGATTGGAGATACCACAAAGAAGAACGAGTATGGATTACCAGGGCACCAGGCATGGAGCCAACAATGAAA ACCAATACCTATGAGAGGGGAACATATTACTTCTTTGACTGTCTTAACTGGAGGAAAGTAGCTAAGGAGT TCCATCTGGAATATGACAAATTAGAAGAACGGCCTCACCTGCCATCCACCTTCAACTACAACCCTGCTCA GCAAGCCTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG232951 representing NM_001199303
Red=Cloning site Green=Tags(s) MVRTDGHTLSEKRNYQVTNSMFGASRKKFVEGVDSDYHDENMYYSQSSMFPHRSEKDMLASPSTSGQLSQ FGASLYGQQSALGLPMRGMSNNTPQLNRSLSQGTQLPSHVTPTTGVPTMSLHTPPSPSRGILPMNPRNMM NHSQVGQGIGIPSRTNSMSSSGLGSPNRSSPSIICMPKQQPSRQPFTVNSMSGFGMNRNQAFGMNNSLSS NIFNGTDGSENVTGLDLSDFPALADRNRREGSGNPTPLINPLAGRAPYVGMVTKPANEQSQDFSIHNEDF PALPGSSYKDPTSSNDDSKSNLNTSGKTTSSTDGPKFPGDKSSTTQNNNQQKKGIQVLPDGRVTNIPQGM VTDQFGMIGLLTFIRAAETDPGMVHLALGSDLTTLGLNLNSPENLYPKFASPWASSPCRPQDIDFHVPSE YLTNIHIRDKLAAIKLGRYGEDLLFYLYYMNGGDVLQLLAAVELFNRDWRYHKEERVWITRAPGMEPTMK TNTYERGTYYFFDCLNWRKVAKEFHLEYDKLEERPHLPSTFNYNPAQQAF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001199303 |
| ORF Size | 1620 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001199303.1, NP_001186232.1 |
| RefSeq Size | 3415 bp |
| RefSeq ORF | 1623 bp |
| Locus ID | 4848 |
| UniProt ID | Q9NZN8 |
| Protein Families | Transcription Factors |
| Protein Pathways | RNA degradation |
| Gene Summary | This gene encodes a subunit of the multi-component CCR4-NOT complex. The CCR4-NOT complex regulates mRNA synthesis and degradation and is also thought to be involved in mRNA splicing, transport and localization. The encoded protein interacts with histone deacetylases and functions as a repressor of polymerase II transcription. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Dec 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
