SSC5D (NM_001195267) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	TurboGFP
Synonyms	S5D-SRCRB
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RG231449 ORF sequence, codon optimized. Due to the complexity of NM_001195267, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAGTCCTGGCCTGTCTGCTGGCCGCACTTGTTGGGATCCAGGCTGTGGAGCGGCTTAGGTTGGCCG ACGGACCCCATGGCTGCGCTGGGAGGCTGGAGGTGTGGCATGGGGGCAGGTGGGGTACTGTATGTGACGA TGGCTGGGATCTGAGGGACGCCGCTGTGGCATGCCGCCAGCTCGGATGTGGGGGCGCCCTTGCAGCACCG GGCGGCGCATTTTTTGGCGAGGGGGCCGGTCCCGTCTGGTTGTCAGAGCTGGCTTGCAGAGGCAACGAGG GCCAGCTCGGTCTGTGTCACCATAGAGGCTGGAAAGCTCACATATGCAGCCACGAGGAGGATGCTGGGGT GGTGTGCGCCGGACAAAGGGTAGCTAACTCCCGCGACGACTCCACAAGCCCTCTTGACGGCGCGCCCTGG CCAGGTCTTCTGCTGGAACTGTCCCCCTCCACTGAGGAGCCTCTGGTAACCCATGCCCCGCGACCTGCAG GCAACCCCCAGAATGCTTCCAGAAAAAAGAGCCCACGCCCCAAGCAAGCCAAATCCACGAGGGCCCCCCT GCTGACTACAGGAGCACCGCGGCAGGAAAGACTGAGACTCGTGAGCGGACCCCATAGATGTGCGGGGAGA CTGGAAGTGTGGCACGGGGGCAGATGGGGGACTGTGTGTGATGATGGTTGGGACCTGAGGGACGCTGCTG TGGCCTGCCGCGAACTCGGGTGCGGTGGTGCGCTTGCCGCTCCTGGTGGGGCTAGGTTCGGACCTGGAGC CGGACCCGTATGGATGGACGACGTTGGCTGCGGAGGCGGTGAACAGGCCCTGCGCGATTGCCCCAGGTCA CCCTGGGGCCGCAGCAATTGCGACCACAGCGAGGATGCCGGCCTGGTCTGTACAGGACCGGCGCCCCGGC TGAGACTGGCTGATGGGCCTCACGGCTGTGCAGGAAGGCTGGAGGTTTGGCATGGTGGACGATGGGGGTC TGTCTGCGACGACGCCTGGGATTTGCGAGATGCGGCTGTAGCCTGCCGCGAACTGGGTTGCGGCGGCGCC TTGGCAGCACCAGGAGGAGCGTTTTTCGGAGAGGGGAGCGGCCCCATCATACTCGACGACTTGAGATGTC GCGGAAACGAAACGGCTCTCCGGTTCTGCCCCGCAAGGCCATGGGGGCAGCATGACTGTCACCACCGCGA GGATGCCGGCGCCGTATGTGACGGGATGCCCTTGGGCTACGTCCCACCAACTGCCCCCACTGATAGTAAC AATTCCACCCCTAGAGAGGCTGCTTCTCGACCACCAAGTACCATGACTTCCCAAGCGCCCGGAACCGCAG GTGTAAGCCCGCCTCCCGCCTCTCCAACAGTGCTGTGGGAACCCGGCCCAGAAGCCGGCTCTCCCCAACT GCGACTCGTTGCCGGCCCGAGCAAATGCAGCGGCCGGCTTGAGGTGTGGCATGATCAGAGGTGGGGCACG GTCTGTGACGATTCCTGGGATATGCGAGACTCAGCGGTTGTCTGTCGGGAACTCGGATGCGGCGGGCCTC AGCAGCCCGATCCAGCCGCAGGAAGGTTCGGCTGGGGGGCCGGGCCTATCTGGCTCGATGACGTTGGGTG CGTGGGCACAGAAGCTTCCCTTTCCGATTGTCCCGCGGCCCCCTGGGGTAAGCATAATTGCGCCCACAAT GAAGACGTTGGAGTGACTTGTACAGGGCCCCCTGGATTGGACTCCATAAGTGATCCTTTTTCATGGTCAT GGATTCCCGGCCTGGGAAGAGATAGGGACGCATGGCTTCCGGGCGAACTCGCCACCAAGCCTAGCGCTAG CGTGACAGCAAGTGTGTTGGAAAAGACGACTACGAAGGCTCCAGGCAAGATGCCGAAAAGTACCAAAAAA TGGGTGACTAAAAATGCAAAGCGCCCCACCACACAGCCGCCTGTGATGCCTACTACCAAACACAGTCGGG CTCAGAGCCCACCCGACCTGACCTCACAGACAACTGCAGCTCTGACAACTGAGGCTAGTAGACGGCCAAC CAGCGAGTTCACTAGACGACCTACAACCGAAGCTCCTCAGCGATGGACCAGTCACACGACCGCGACACTC ACCCCCCAGGCCCCAAGAGAAAGGACTACAAAAACAATGGCAATGCTCACCACACAGGGCCCGCAGGAGA TGACCAGCGAATCTACAATCAAGAGTATCCCACAGGCTAGTCTGGAGCCCAGCGCGGAGATCCCTGAAGG CTCACCTGAATCACCCAAAGATCCTGCGCCATCCCCTTCCGTTTCAACAACAGGCGAGAGCGGCCTTTTT AGAGTTCGGCTGGCTGATGGCCCTAACAGGTGCGCCGGGCGGCTTGAGGTGTGGCATGCGGGCCGGTGGG GTACAGTGTGCGATGATAACTGGGATCTTAGGGACGCCACGGTCGCTTGCTGGGAGCTGGGTTGCGGCAA GGTAAGGCCAAGGGTGGGGAAGACTCACTACGGCCCTGGGACCGGTCCGATTTGGCTCGACGACATGGGC TGTAAGGGAAGTGAGGCGTCACTGTCTGACTGCCCATCTGGAGCCTGGGGTAAGCACAACTGTGACCATG AGGAGGACGTGGGCTTGACCTGCACTGGTTACACTGATTACGACGACTATCCTCCTTGGACTTGGGACCC CACCTCCCGGGAGGACCTCGCGAAAGGAACGACCACCGCAGGCGTCCCGGGCCACACCCTTCCTTGGCGA ACAACCAGACGCCCCGGCAGCAGCTCACCAGCGATCAGGAGGCTGCCTGATACTGAGCCCGAAGCAGGAG CCCCTAGAGGAGACGCACCCAGGAGTAGAACCGCTAGAGTAGCTGCACCCCCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >RG231449 representing NM_001195267 Red=Cloning site Green=Tags(s) MRVLACLLAALVGIQAVERLRLADGPHGCAGRLEVWHGGRWGTVCDDGWDLRDAAVACRQLGCGGALAAP GGAFFGEGAGPVWLSELACRGNEGQLGLCHHRGWKAHICSHEEDAGVVCAGQRVANSRDDSTSPLDGAPW PGLLLELSPSTEEPLVTHAPRPAGNPQNASRKKSPRPKQAKSTRAPLLTTGAPRQERLRLVSGPHRCAGR LEVWHGGRWGTVCDDGWDLRDAAVACRELGCGGALAAPGGARFGPGAGPVWMDDVGCGGGEQALRDCPRS PWGRSNCDHSEDAGLVCTGPAPRLRLADGPHGCAGRLEVWHGGRWGSVCDDAWDLRDAAVACRELGCGGA LAAPGGAFFGEGSGPIILDDLRCRGNETALRFCPARPWGQHDCHHREDAGAVCDGMPLGYVPPTAPTDSN NSTPREAASRPPSTMTSQAPGTAGVSPPPASPTVLWEPGPEAGSPQLRLVAGPSKCSGRLEVWHDQRWGT VCDDSWDMRDSAVVCRELGCGGPQQPDPAAGRFGWGAGPIWLDDVGCVGTEASLSDCPAAPWGKHNCAHN EDVGVTCTGPPGLDSISDPFSWSWIPGLGRDRDAWLPGELATKPSASVTASVLEKTTTKAPGKMPKSTKK WVTKNAKRPTTQPPVMPTTKHSRAQSPPDLTSQTTAALTTEASRRPTSEFTRRPTTEAPQRWTSHTTATL TPQAPRERTTKTMAMLTTQGPQEMTSESTIKSIPQASLEPSAEIPEGSPESPKDPAPSPSVSTTGESGLF RVRLADGPNRCAGRLEVWHAGRWGTVCDDNWDLRDATVACWELGCGKVRPRVGKTHYGPGTGPIWLDDMG CKGSEASLSDCPSGAWGKHNCDHEEDVGLTCTGYTDYDDYPPWTWDPTSREDLAKGTTTAGVPGHTLPWR TTRRPGSSSPAIRRLPDTEPEAGAPRGDAPRSRTARVAAPP TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001195267
ORF Size	2853 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001195267.1, NP_001182196.1
RefSeq Size	3081 bp
RefSeq ORF	2856 bp
Locus ID	284297
UniProt ID	A1L4H1
Gene Summary	Binds to extracellular matrix proteins. Binds to pathogen-associated molecular patterns (PAMPs) present on the cell walls of Gram-positive and Gram-negative bacteria and fungi, behaving as a pattern recognition receptor (PRR). Induces bacterial and fungal aggregation and subsequent inhibition of PAMP-induced cytokine release. Does not possess intrinsic bactericidal activity. May play a role in the innate defense and homeostasis of certain epithelial surfaces (By similarity).[UniProtKB/Swiss-Prot Function]

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