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CPNE1 (NM_003915) Human Tagged ORF Clone
CAT＃: RG229279
- TrueORF®
CPNE1 (tGFP-tagged) - Human copine I (CPNE1), transcript variant 3
ORF Plasmid: DDK
"NM_003915" in other vectors (5)
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CNY 5,230.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | COPN1; CPN1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG229279 representing NM_003915
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGATGATGCACATGGCCCACTGCGTGACCTTGGTTCAGCTGTCCATTTCCTGTGACCATCTCATTG ACAAGGACATCGGCTCCAAGTCTGACCCACTCTGCGTCCTTTTACAGGATGTGGGAGGGGGCAGCTGGGC TGAGCTTGGCCGGACTGAACGGGTGCGGAACTGCTCAAGCCCTGAGTTCTCCAAGACTCTACAGCTTGAG TACCGCTTTGAGACAGTCCAGAAGCTACGCTTTGGAATCTATGACATAGACAACAAGACGCCAGAGCTGA GGGATGATGACTTCCTAGGGGGTGCTGAGTGTTCCCTAGGACAGATTGTGTCCAGCCAGGTACTGACTCT CCCCTTGATGCTGAAGCCTGGAAAACCTGCTGGGCGGGGGACCATCACGGTCTCAGCTCAGGAATTAAAG GACAATCGTGTAGTAACCATGGAGGTAGAGGCCAGAAACCTAGATAAGAAGGACTTCCTGGGAAAATCAG ATCCATTTCTGGAGTTCTTCCGCCAGGGTGATGGGAAATGGCACCTGGTGTACAGATCTGAGGTCATCAA GAACAACCTGAACCCTACATGGAAGCGTTTCTCAGTCCCCGTTCAGCATTTCTGTGGTGGGAACCCCAGC ACACCCATCCAGGTGCAATGCTCCGATTATGACAGTGACGGGTCACATGATCTCATCGGTACCTTCCACA CCAGCTTGGCCCAGCTGCAGGCAGTCCCGGCTGAGTTTGAATGCATCCACCCTGAGAAGCAGCAGAAAAA GAAAAGCTACAAGAACTCTGGAACTATCCGTGTCAAGATTTGTCGGGTAGAAACAGAGTACTCCTTTCTG GACTATGTGATGGGAGGCTGTCAGATCAACTTCACTGTGGGCGTGGACTTCACTGGCTCCAATGGAGACC CCTCCTCACCTGACTCCCTACACTACCTGAGTCCAACAGGGGTCAATGAGTACCTGATGGCACTGTGGAG TGTGGGCAGCGTGGTTCAGGACTATGACTCAGACAAGCTGTTCCCTGCATTTGGATTTGGGGCCCAGGTT CCCCCTGACTGGCAGGTCTCGCATGAATTTGCCTTGAATTTCAACCCCAGTAACCCCTACTGTGCAGGCA TCCAGGGCATTGTGGATGCCTACCGCCAAGCCCTGCCCCAAGTTCGCCTCTATGGCCCTACCAACTTTGC ACCCATCATCAACCATGTGGCCAGGTTTGCAGCCCAGGCTGCACATCAGGGGACTGCCTCGCAATACTTC ATGCTGTTGCTGCTGACTGATGGTGCTGTGACGGATGTGGAAGCCACACGTGAGGCTGTGGTGCGTGCCT CGAACCTGCCCATGTCAGTGATCATTGTGGGTGTGGGTGGTGCTGACTTTGAGGCCATGGAGCAGCTGGA CGCTGATGGTGGACCCCTGCATACACGTTCTGGGCAGGCTGCTGCCCGCGACATTGTGCAGTTTGTACCC TACCGCCGGTTCCAGAATGCCCCTCGGGAGGCATTGGCACAGACCGTGCTCGCAGAAGTGCCCACACAAC TGGTCTCATACTTCAGGGCCCAGGGTTGGGCCCCGCTCAAGCCACTTCCACCCTCAGCCAAGGATCCTGC ACAGGCCCCCCAGGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG229279 representing NM_003915
Red=Cloning site Green=Tags(s) MKMMHMAHCVTLVQLSISCDHLIDKDIGSKSDPLCVLLQDVGGGSWAELGRTERVRNCSSPEFSKTLQLE YRFETVQKLRFGIYDIDNKTPELRDDDFLGGAECSLGQIVSSQVLTLPLMLKPGKPAGRGTITVSAQELK DNRVVTMEVEARNLDKKDFLGKSDPFLEFFRQGDGKWHLVYRSEVIKNNLNPTWKRFSVPVQHFCGGNPS TPIQVQCSDYDSDGSHDLIGTFHTSLAQLQAVPAEFECIHPEKQQKKKSYKNSGTIRVKICRVETEYSFL DYVMGGCQINFTVGVDFTGSNGDPSSPDSLHYLSPTGVNEYLMALWSVGSVVQDYDSDKLFPAFGFGAQV PPDWQVSHEFALNFNPSNPYCAGIQGIVDAYRQALPQVRLYGPTNFAPIINHVARFAAQAAHQGTASQYF MLLLLTDGAVTDVEATREAVVRASNLPMSVIIVGVGGADFEAMEQLDADGGPLHTRSGQAAARDIVQFVP YRRFQNAPREALAQTVLAEVPTQLVSYFRAQGWAPLKPLPPSAKDPAQAPQA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003915 |
| ORF Size | 1626 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003915.5 |
| RefSeq Size | 2211 bp |
| RefSeq ORF | 1629 bp |
| Locus ID | 8904 |
| UniProt ID | Q99829 |
| Domains | C2, VWA |
| Gene Summary | Calcium-dependent membrane-binding proteins may regulate molecular events at the interface of the cell membrane and cytoplasm. This gene encodes a calcium-dependent protein that also contains two N-terminal type II C2 domains and an integrin A domain-like sequence in the C-terminus. However, the encoded protein does not contain a predicted signal sequence or transmembrane domains. This protein has a broad tissue distribution and it may function in membrane trafficking. This gene and the gene for RNA binding motif protein 12 overlap at map location 20q11.21. Alternate splicing results in multiple transcript variants encoding different proteins. [provided by RefSeq, Aug 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC229279 | CPNE1 (Myc-DDK-tagged)-Human copine I (CPNE1), transcript variant 3 |
CNY 4,040.00 |
|
| RC229279L3 | Lenti-ORF clone of CPNE1 (Myc-DDK-tagged)-Human copine I (CPNE1), transcript variant 3 |
CNY 6,650.00 |
|
| RC229279L4 | Lenti-ORF clone of CPNE1 (mGFP-tagged)-Human copine I (CPNE1), transcript variant 3 |
CNY 6,650.00 |
|
| SC117680 | CPNE1 (untagged)-Human copine I (CPNE1), transcript variant 3 |
CNY 4,008.00 |
|
| SC327914 | CPNE1 (untagged)-Human copine I (CPNE1) transcript variant 3 |
CNY 8,650.00 |
