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ZBTB20 (NM_001164342) Human Tagged ORF Clone
CAT＃: RG228567
- TrueORF®
ZBTB20 (tGFP-tagged) - Human zinc finger and BTB domain containing 20 (ZBTB20), transcript variant 1
ORF Plasmid: DDK
"NM_001164342" in other vectors (4)
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CNY 8,550.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | DPZF; HOF; ODA-8S; PRIMS; ZNF288 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG228567 representing NM_001164342
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTAGAACGGAAGAAACCCAAGACAGCTGAAAACCAGAAGGCATCTGAGGAGAATGAGATTACTCAGC CGGGTGGATCCAGCGCCAAGCCGGGCCTTCCCTGCCTGAACTTTGAAGCTGTTTTGTCTCCAGACCCAGC CCTCATCCACTCAACACATTCACTGACAAACTCTCACGCTCACACCGGGTCATCTGATTGTGACATCAGT TGCAAGGGGATGACCGAGCGCATTCACAGCATCAACCTTCACAACTTCAGCAATTCCGTGCTCGAGACCC TCAACGAGCAGCGCAACCGTGGCCACTTCTGTGACGTAACGGTGCGCATCCACGGGAGCATGCTGCGCGC ACACCGCTGCGTGCTGGCAGCCGGCAGCCCCTTCTTCCAGGACAAACTGCTGCTTGGCTACAGCGACATC GAGATCCCGTCGGTGGTGTCAGTGCAGTCAGTGCAAAAGCTCATTGACTTCATGTACAGCGGCGTGCTAC GGGTCTCGCAGTCGGAAGCTCTGCAGATCCTCACGGCCGCCAGCATCCTGCAGATCAAAACAGTCATCGA CGAGTGCACGCGCATCGTGTCACAGAACGTGGGCGATGTGTTCCCGGGGATCCAGGACTCGGGCCAGGAC ACGCCGCGGGGCACTCCCGAGTCAGGCACGTCAGGCCAGAGCAGCGACACGGAGTCGGGCTACCTGCAGA GCCACCCACAGCACAGCGTGGACAGGATCTACTCGGCACTCTACGCGTGCTCCATGCAGAATGGCAGCGG CGAGCGCTCTTTTTACAGCGGCGCAGTGGTCAGCCACCACGAGACTGCGCTCGGCCTGCCCCGCGACCAC CACATGGAAGACCCCAGCTGGATCACACGCATCCATGAGCGCTCGCAGCAGATGGAGCGCTACCTGTCCA CCACCCCCGAGACCACGCACTGCCGCAAGCAGCCCCGGCCTGTGCGCATCCAGACCCTAGTGGGCAACAT CCACATCAAGCAGGAGATGGAGGACGATTACGACTACTACGGGCAGCAAAGGGTGCAGATCCTGGAACGC AACGAATCCGAGGAGTGCACGGAAGACACAGACCAGGCCGAGGGCACCGAGAGTGAGCCCAAAGGTGAAA GCTTCGACTCGGGCGTCAGCTCCTCCATAGGCACCGAGCCTGACTCGGTGGAGCAGCAGTTTGGGCCTGG GGCGGCGCGGGACAGCCAGGCTGAACCCACCCAACCCGAGCAGGCTGCAGAAGCCCCCGCTGAGGGTGGT CCGCAGACAAACCAGCTAGAAACAGGTGCTTCCTCTCCGGAGAGAAGCAATGAAGTGGAGATGGACAGCA CTGTTATCACTGTCAGCAACAGCTCCGACAAGAGCGTCCTACAACAGCCTTCGGTCAACACGTCCATCGG GCAGCCATTGCCAAGTACCCAGCTCTACTTACGCCAGACAGAAACCCTCACCAGCAACCTGAGGATGCCT CTGACCTTGACCAGCAACACGCAGGTCATTGGCACAGCTGGCAACACCTACCTGCCAGCCCTCTTCACTA CCCAGCCCGCGGGCAGTGGCCCCAAGCCTTTCCTCTTCAGCCTGCCACAGCCCCTGGCAGGCCAGCAGAC CCAGTTTGTGACAGTGTCCCAGCCCGGTCTGTCGACCTTTACTGCACAGCTGCCAGCGCCACAGCCCCTG GCCTCATCCGCAGGCCACAGCACAGCCAGTGGGCAAGGCGAAAAAAAGCCTTATGAGTGCACTCTCTGCA ACAAGACTTTCACCGCCAAACAGAACTACGTCAAGCACATGTTCGTACACACAGGTGAGAAGCCCCACCA ATGCAGCATCTGTTGGCGCTCCTTCTCCTTAAAGGATTACCTTATCAAGCACATGGTGACACACACAGGA GTGAGGGCATACCAGTGTAGTATCTGCAACAAGCGCTTCACCCAGAAGAGCTCCCTCAACGTGCACATGC GCCTCCACCGGGGAGAGAAGTCCTACGAGTGCTACATCTGCAAAAAGAAGTTCTCTCACAAGACCCTCCT GGAGCGACACGTGGCCCTGCACAGTGCCAGCAATGGGACCCCCCCTGCAGGCACACCCCCAGGTGCCCGC GCTGGCCCCCCAGGCGTGGTGGCCTGCACGGAGGGGACCACTTACGTCTGCTCCGTCTGCCCAGCAAAGT TTGACCAAATCGAGCAGTTCAACGACCACATGAGGATGCATGTGTCTGACGGA AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG228567 representing NM_001164342
Red=Cloning site Green=Tags(s) MLERKKPKTAENQKASEENEITQPGGSSAKPGLPCLNFEAVLSPDPALIHSTHSLTNSHAHTGSSDCDIS CKGMTERIHSINLHNFSNSVLETLNEQRNRGHFCDVTVRIHGSMLRAHRCVLAAGSPFFQDKLLLGYSDI EIPSVVSVQSVQKLIDFMYSGVLRVSQSEALQILTAASILQIKTVIDECTRIVSQNVGDVFPGIQDSGQD TPRGTPESGTSGQSSDTESGYLQSHPQHSVDRIYSALYACSMQNGSGERSFYSGAVVSHHETALGLPRDH HMEDPSWITRIHERSQQMERYLSTTPETTHCRKQPRPVRIQTLVGNIHIKQEMEDDYDYYGQQRVQILER NESEECTEDTDQAEGTESEPKGESFDSGVSSSIGTEPDSVEQQFGPGAARDSQAEPTQPEQAAEAPAEGG PQTNQLETGASSPERSNEVEMDSTVITVSNSSDKSVLQQPSVNTSIGQPLPSTQLYLRQTETLTSNLRMP LTLTSNTQVIGTAGNTYLPALFTTQPAGSGPKPFLFSLPQPLAGQQTQFVTVSQPGLSTFTAQLPAPQPL ASSAGHSTASGQGEKKPYECTLCNKTFTAKQNYVKHMFVHTGEKPHQCSICWRSFSLKDYLIKHMVTHTG VRAYQCSICNKRFTQKSSLNVHMRLHRGEKSYECYICKKKFSHKTLLERHVALHSASNGTPPAGTPPGAR AGPPGVVACTEGTTYVCSVCPAKFDQIEQFNDHMRMHVSDG SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001164342 |
| ORF Size | 2223 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001164342.2 |
| RefSeq Size | 3317 bp |
| RefSeq ORF | 2226 bp |
| Locus ID | 26137 |
| UniProt ID | Q9HC78 |
| Protein Families | Transcription Factors |
| Gene Summary | This gene, which was initially designated as dendritic cell-derived BTB/POZ zinc finger (DPZF), belongs to a family of transcription factors with an N-terminal BTB/POZ domain and a C-terminal DNA-bindng zinc finger domain. The BTB/POZ domain is a hydrophobic region of approximately 120 aa which mediates association with other BTB/POZ domain-containing proteins. This gene acts as a transcriptional repressor and plays a role in many processes including neurogenesis, glucose homeostasis, and postnatal growth. Mutations in this gene have been associated with Primrose syndrome as well as the 3q13.31 microdeletion syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC228567 | ZBTB20 (Myc-DDK-tagged)-Human zinc finger and BTB domain containing 20 (ZBTB20), transcript variant 1 |
CNY 8,144.00 |
|
| RC228567L3 | Lenti-ORF clone of ZBTB20 (Myc-DDK-tagged)-Human zinc finger and BTB domain containing 20 (ZBTB20), transcript variant 1 |
CNY 9,690.00 |
|
| RC228567L4 | Lenti-ORF clone of ZBTB20 (mGFP-tagged)-Human zinc finger and BTB domain containing 20 (ZBTB20), transcript variant 1 |
CNY 9,690.00 |
|
| SC327202 | ZBTB20 (untagged)-Human zinc finger and BTB domain containing 20 (ZBTB20) transcript variant 1 |
CNY 8,152.00 |
