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TIAM2 (NM_012454) Human Tagged ORF Clone
CAT＃: RG222386
- TrueORF®
TIAM2 (tGFP-tagged) - Human T-cell lymphoma invasion and metastasis 2 (TIAM2), transcript variant 1
ORF Plasmid: DDK
"NM_012454" in other vectors (2)
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CNY 18,720.00
货期*
8周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | STEF; TIAM-2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG222386 representing NM_012454
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAACTCCGACAGTCAGTACACCCTTCAAGGATCTAAAAATCATAGCAATACTATTACTGGTGCTA AGCAAATTCCTTGCTCCCTGAAAATACGTGGCATTCATGCAAAAGAGGAAAAGTCATTGCATGGATGGGG TCACGGAAGCAACGGAGCAGGTTACAAGTCCAGGTCCCTGGCCCGAAGCTGCCTTTCTCACTTTAAGAGT AACCAGCCTTACGCATCGAGACTCGGTGGCCCCACATGCAAGGTCTCCAGAGGTGTTGCCTACTCCACGC ACAGGACAAATGCCCCAGGGAAGGATTTCCAGGGCATCAGTGCTGCTTTCTCAACTGAGAATGGCTTCCA CTCTGTTGGCCACGAGCTGGCAGATAACCACATCACCTCCAGAGACTGCAACGGACACCTTCTCAACTGC TACGGGAGGAATGAGAGCATTGCCTCCACCCCACCGGGCGAAGACCGCAAGAGCCCCCGAGTGCTCATCA AAACGCTGGGGAAGCTGGATGGGTGTTTAAGGGTCGAGTTCCACAATGGTGGCAACCCCAGCAAAGTGCC TGCAGAGGACTGCAGTGAGCCGGTGCAGCTGCTGAGGTACTCACCTACCTTAGCATCGGAAACCTCCCCT GTGCCTGAAGCCAGGAGGGGGTCCAGCGCCGATTCCCTGCCCAGCCATCGCCCCTCTCCCACGGACTCTC GCCTGCGGTCCAGCAAAGGCAGCTCCCTGAGTTCTGAGTCATCCTGGTACGACTCCCCTTGGGGCAATGC TGGAGAGCTGAGCGAGGCTGAGGGCTCCTTCCTGGCCCCCGGCATGCCTGACCCCAGTCTCCATGCCAGC TTCCCACCTGGCGATGCCAAAAAGCCTTTCAACCAAAGCTCTTCCCTCTCCTCCCTCCGGGAACTGTACA AAGATGCCAACCTGGGGAGCCTCTCCCCCTCAGGTATCCGCCTTTCTGATGAATACATGGGCACGCATGC CAGCCTGAGCAACCGTGTCTCTTTTGCTTCCGACATTGATGTGCCCTCCAGAGTGGCACACGGGGACCCC ATCCAGTACAGTTCCTTCACTCTCCCCTGTCGGAAGCCCAAAGCCTTTGTTGAGGATACTGCGAAGAAGG ACTCCCTCAAAGCCAGGATGCGACGGATCAGTGACTGGACGGGAAGCCTCTCAAGGAAGAAAAGGAAACT CCAGGAGCCGAGGTCCAAGGAGGGCAGTGACTACTTTGACAGTCGCTCTGATGGACTGAATACAGATGTG CAGGGATCCTCCCAGGCATCTGCTTTTCTGTGGTCAGGGGGCTCTACTCAGATCCTGTCTCAGAGAAGTG AATCCACACATGCGATTGGCAGCGATCCCCTCCGGCAGAACATTTATGAGAATTTCATGCGAGAGTTGGA AATGAGCAGGACCAACACTGAGAACATAGAAACATCTACAGAAACCGCCGAGTCCAGCAGCGAGTCACTC AGCTCTCTGGAACAGCTGGATCTGCTCTTTGAGAAGGAACAGGGGGTGGTCCGGAAGGCCGGGTGGCTCT TCTTCAAGCCCCTGGTCACTGTGCAGAAGGAAAGGAAGCTTGAGCTGGTGGCACGAAGGAAATGGAAACA GTACTGGGTAACGCTGAAAGGATGCACGCTGCTGTTTTATGAGACCTATGGGAAGAATTCCATGGATCAG AGCAGTGCCCCTCGGTGTGCTCTGTTTGCAGAAGACAGCATAGTGCAGTCTGTTCCAGAGCATCCCAAGA AAGAAAATGTGTTCTGCCTCAGCAACTCCTTTGGAGATGTCTACCTTTTCCAGGCCACCAGCCAGACAGA TCTAGAAAACTGGGTCACTGCTGTACACTCTGCTTGTGCATCCCTTTTTGCAAAGAAGCATGGGAAAGAG GACACGCTGCGGCTGCTGAAGAACCAGACCAAAAACCTGCTTCAGAAGATAGACATGGACAGCAAGATGA AGAAGATGGCAGAGCTGCAGCTGTCCGTGGTGAGCGACCCAAAGAACAGGAAAGCCATAGAGAACCAGAT CCAGCAATGGGAGCAGAATCTTGAGAAATTTCACATGGATCTGTTCAGGATGCGCTGCTATCTGGCCAGC CTACAAGGTGGGGAGTTACCGAACCCAAAGAGTCTCCTTGCAGCCGCCAGCCGCCCCTCCAAGCTGGCCC TCGGCAGGCTGGGCATCTTGTCTGTTTCCTCTTTCCATGCTCTGGTATGTTCTAGAGATGACTCTGCTCT CCGGAAAAGGACACTGTCACTGACCCAGCGAGGGAGAAACAAGAAGGGAATATTTTCTTCGTTAAAAGGG CTGGACACACTGGCCAGAAAAGGCAAGGAGAAGAGACCTTCTATAACTCAGGTCGATGAACTTCTGCATA TATATGGTTCAACAGTAGACGGTGTTCCCCGAGACAATGCATGGGAAATCCAGACTTATGTCCACTTTCA GGACAATCACGGAGTTACTGTAGGGATCAAGCCAGAGCACAGAGTAGAAGATATTTTGACTTTGGCATGC AAGATGAGGCAGTTGGAACCCAGCCATTATGGCCTACAGCTTCGAAAATTAGTAGATGACAATGTTGAGT ATTGCATCCCTGCACCATATGAATATATGCAACAACAGGTTTATGATGAAATAGAAGTCTTTCCACTAAA TGTTTATGACGTGCAGCTCACGAAGACTGGGAGTGTGTGTGACTTTGGGTTTGCAGTTACAGCGCAGGTG GATGAGCGTCAGCATCTCAGCCGGATATTTATAAGCGACGTTCTTCCCGATGGCCTGGCGTATGGGGAAG GGCTGAGAAAGGGCAATGAGATCATGACCTTAAATGGGGAAGCTGTGTCTGATCTTGACCTTAAGCAGAT GGAGGCCCTGTTTTCTGAGAAGAGCGTCGGACTCACTCTGATTGCCCGGCCTCCGGACACAAAAGCAACC CTGTGTACATCCTGGTCAGACAGTGACCTGTTCTCCAGGGACCAGAAGAGTCTGCTGCCCCCTCCTAACC AGTCCCAACTGCTGGAGGAATTCCTGGATAACTTTAAAAAGAATACAGCCAATGATTTCAGCAACGTCCC TGATATCACAACAGGTCTGAAAAGGAGTCAGACAGATGGCACTCTGGATCAGGTTTCCCACAGGGAGAAA ATGGAGCAGACATTCAGGAGTGCTGAGCAGATCACTGCACTGTGCAGGAGTTTTAACGACAGTCAGGCCA ACGGCATGGAAGGACCGCGGGAGAATCAGGATCCTCCTCCGAGGCCTCTGGCCCGCCACCTGTCTGATGC AGACCGCCTCCGCAAAGTCATCCAGGAGCTTGTGGACACAGAGAAGTCCTACGTGAAGGATTTGAGCTGC CTCTTTGAATTATACTTGGAGCCACTTCAGAATGAGACCTTTCTTACCCAAGATGAGATGGAGTCACTTT TTGGAAGTTTGCCAGAGATGCTTGAGTTTCAGAAGGTGTTTCTGGAGACCCTGGAGGATGGGATTTCAGC ATCATCTGACTTTAACACCCTAGAAACCCCCTCACAGTTTAGAAAATTACTGTTTTCCCTTGGAGGCTCT TTCCTTTATTACGCGGACCACTTTAAACTGTACAGTGGATTCTGTGCTAACCATATCAAAGTACAGAAGG TTCTGGAGCGAGCTAAAACTGACAAAGCCTTCAAGGCTTTTCTGGACGCCCGGAACCCCACCAAGCAGCA TTCCTCCACGCTGGAGTCCTACCTCATCAAGCCGGTTCAGAGAGTGCTCAAGTACCCGCTGCTGCTCAAG GAGCTGGTGTCCCTGACGGACCAGGAGAGCGAGGAGCACTACCACCTGACGGAAGCACTAAAGGCAATGG AGAAAGTAGCGAGCCACATCAATGAGATGCAGAAGATCTATGAGGATTATGGGACCGTGTTTGACCAGCT AGTAGCTGAGCAGAGCGGAACAGAGAAGGAGGTAACAGAACTTTCGATGGGAGAGCTTCTGATGCACTCT ACGGTTTCCTGGTTGAATCCATTTCTGTCTCTAGGAAAAGCTAGAAAGGACCTTGAGCTCACAGTATTTG TTTTTAAGAGAGCCGTCATACTGGTTTATAAAGAAAACTGCAAACTGAAAAAGAAATTGCCCTCGAATTC CCGGCCTGCACACAACTCTACTGACTTGGACCCATTTAAATTCCGCTGGTTGATCCCCATCTCCGCGCTT CAAGTCAGACTGGGGAATCCAGCAGGGACAGAAAATAATTCCATATGGGAACTGATCCATACGAAGTCAG AAATAGAAGGACGGCCAGAAACCATCTTTCAGTTGTGTTGCAGTGACAGTGAAAGCAAAACCAACATTGT TAAGGTGATTCGTTCTATTCTGAGGGAGAACTTCAGGCGTCACATAAAGTGTGAATTACCACTGGAGAAA ACGTGTAAGGATCGCCTGGTACCTCTTAAGAACCGAGTTCCTGTTTCGGCCAAATTAGCTTCATCCAGGT CTTTAAAAGTCCTGAAGAATTCCTCCAGCAACGAGTGGACCGGTGAGACTGGCAAGGGAACCTTGCTGGA CTCTGACGAGGGCAGCTTGAGCAGCGGCACCCAGAGCAGCGGCTGCCCCACGGCTGAGGGCAGGCAGGAC TCCAAGAGCACTTCTCCCGGGAAATACCCACACCCCGGCTTGGCAGATTTTGCCGACAATCTCATCAAAG AGAGTGACATCCTGAGCGATGAAGATGATGACCACCGTCAGACTGTGAAGCAGGGCAGCCCTACTAAAGA CATCGAAATTCAGTTCCAGAGACTGAGGATTTCCGAGGACCCAGACGTTCACCCCGAGGCTGAGCAGCAG CCTGGCCCGGAGTCGGGTGAGGGTCAGAAAGGAGGAGAGCAGCCCAAACTGGTCCGGGGGCACTTCTGCC CCATTAAACGAAAAGCCAACAGCACCAAGAGGGACAGAGGAACTTTGCTCAAGGCGCAGATCCGTCACCA GTCCCTTGACAGTCAGTCTGAAAATGCCACCATCGACCTAAATTCTGTTCTAGAGCGAGAATTCAGTGTC CAGAGTTTAACATCTGTTGTCAGTGAGGAGTGTTTTTATGAAACAGAGAGCCACGGAAAATCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG222386 representing NM_012454
Red=Cloning site Green=Tags(s) MGNSDSQYTLQGSKNHSNTITGAKQIPCSLKIRGIHAKEEKSLHGWGHGSNGAGYKSRSLARSCLSHFKS NQPYASRLGGPTCKVSRGVAYSTHRTNAPGKDFQGISAAFSTENGFHSVGHELADNHITSRDCNGHLLNC YGRNESIASTPPGEDRKSPRVLIKTLGKLDGCLRVEFHNGGNPSKVPAEDCSEPVQLLRYSPTLASETSP VPEARRGSSADSLPSHRPSPTDSRLRSSKGSSLSSESSWYDSPWGNAGELSEAEGSFLAPGMPDPSLHAS FPPGDAKKPFNQSSSLSSLRELYKDANLGSLSPSGIRLSDEYMGTHASLSNRVSFASDIDVPSRVAHGDP IQYSSFTLPCRKPKAFVEDTAKKDSLKARMRRISDWTGSLSRKKRKLQEPRSKEGSDYFDSRSDGLNTDV QGSSQASAFLWSGGSTQILSQRSESTHAIGSDPLRQNIYENFMRELEMSRTNTENIETSTETAESSSESL SSLEQLDLLFEKEQGVVRKAGWLFFKPLVTVQKERKLELVARRKWKQYWVTLKGCTLLFYETYGKNSMDQ SSAPRCALFAEDSIVQSVPEHPKKENVFCLSNSFGDVYLFQATSQTDLENWVTAVHSACASLFAKKHGKE DTLRLLKNQTKNLLQKIDMDSKMKKMAELQLSVVSDPKNRKAIENQIQQWEQNLEKFHMDLFRMRCYLAS LQGGELPNPKSLLAAASRPSKLALGRLGILSVSSFHALVCSRDDSALRKRTLSLTQRGRNKKGIFSSLKG LDTLARKGKEKRPSITQVDELLHIYGSTVDGVPRDNAWEIQTYVHFQDNHGVTVGIKPEHRVEDILTLAC KMRQLEPSHYGLQLRKLVDDNVEYCIPAPYEYMQQQVYDEIEVFPLNVYDVQLTKTGSVCDFGFAVTAQV DERQHLSRIFISDVLPDGLAYGEGLRKGNEIMTLNGEAVSDLDLKQMEALFSEKSVGLTLIARPPDTKAT LCTSWSDSDLFSRDQKSLLPPPNQSQLLEEFLDNFKKNTANDFSNVPDITTGLKRSQTDGTLDQVSHREK MEQTFRSAEQITALCRSFNDSQANGMEGPRENQDPPPRPLARHLSDADRLRKVIQELVDTEKSYVKDLSC LFELYLEPLQNETFLTQDEMESLFGSLPEMLEFQKVFLETLEDGISASSDFNTLETPSQFRKLLFSLGGS FLYYADHFKLYSGFCANHIKVQKVLERAKTDKAFKAFLDARNPTKQHSSTLESYLIKPVQRVLKYPLLLK ELVSLTDQESEEHYHLTEALKAMEKVASHINEMQKIYEDYGTVFDQLVAEQSGTEKEVTELSMGELLMHS TVSWLNPFLSLGKARKDLELTVFVFKRAVILVYKENCKLKKKLPSNSRPAHNSTDLDPFKFRWLIPISAL QVRLGNPAGTENNSIWELIHTKSEIEGRPETIFQLCCSDSESKTNIVKVIRSILRENFRRHIKCELPLEK TCKDRLVPLKNRVPVSAKLASSRSLKVLKNSSSNEWTGETGKGTLLDSDEGSLSSGTQSSGCPTAEGRQD SKSTSPGKYPHPGLADFADNLIKESDILSDEDDDHRQTVKQGSPTKDIEIQFQRLRISEDPDVHPEAEQQ PGPESGEGQKGGEQPKLVRGHFCPIKRKANSTKRDRGTLLKAQIRHQSLDSQSENATIDLNSVLEREFSV QSLTSVVSEECFYETESHGKS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012454 |
| ORF Size | 5103 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012454.3, NP_036586.2 |
| RefSeq Size | 5916 bp |
| RefSeq ORF | 5106 bp |
| Locus ID | 26230 |
| UniProt ID | Q8IVF5 |
| Domains | RhoGEF, PDZ, RBD |
| Protein Families | Druggable Genome |
| Protein Pathways | Chemokine signaling pathway, Regulation of actin cytoskeleton |
| Gene Summary | This gene encodes a guanine nucleotide exchange factor. A highly similar mouse protein specifically activates ras-related C3 botulinum substrate 1, converting this Rho-like guanosine triphosphatase (GTPase) from a guanosine diphosphate-bound inactive state to a guanosine triphosphate-bound active state. The encoded protein may play a role in neural cell development. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008] |
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