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KDM5A (NM_001042603) Human Tagged ORF Clone
CAT＃: RG221461
- TrueORF®
KDM5A (tGFP-tagged) - Human lysine (K)-specific demethylase 5A (KDM5A)
ORF Plasmid: DDK
"NM_001042603" in other vectors (4)
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CNY 18,640.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | RBBP-2; RBBP2; RBP2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG221461 representing NM_001042603
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGCGTGGGGCCGGGGGGCTACGCGGCGGAGTTCGTGCCACCGCCAGAGTGCCCCGTCTTTGAGC CGAGTTGGGAGGAGTTCACAGATCCGCTCAGCTTTATCGGCCGCATCCGGCCTTTGGCGGAGAAAACCGG CATCTGCAAAATTCGGCCGCCCAAGGACTGGCAGCCTCCATTTGCCTGTGAAGTAAAAAGCTTTCGTTTC ACTCCAAGAGTCCAGCGCCTGAATGAACTTGAGGCAATGACCAGAGTGAGATTGGATTTCTTGGATCAAC TAGCAAAATTTTGGGAACTTCAAGGATCTACTCTGAAGATCCCTGTGGTAGAGAGAAAAATCCTGGATCT GTATGCTTTGAGCAAGATTGTTGCCAGCAAAGGAGGTTTTGAAATGGTCACCAAAGAGAAGAAATGGTCT AAAGTGGGTAGTCGCTTGGGATATCTGCCAGGAAAAGGAACTGGGTCTCTTTTGAAGTCACATTATGAAA GAATTCTCTACCCATATGAGCTTTTCCAGTCTGGTGTGAGCCTTATGGGTGTGCAGATGCCTAATTTAGA TCTTAAAGAAAAAGTGGAGCCTGAGGTTCTCAGCACTGATACCCAAACTTCCCCAGAGCCAGGCACAAGG ATGAACATTCTGCCGAAGAGAACAAGACGTGTGAAGACTCAGTCAGAATCTGGAGATGTGAGTAGAAACA CGGAACTGAAGAAACTTCAGATTTTTGGGGCTGGGCCCAAGGTTGTGGGCTTGGCAATGGGAACAAAAGA TAAAGAAGATGAGGTCACCCGAAGACGAAAAGTTACCAACAGGTCAGACGCATTTAACATGCAAATGAGA CAACGGAAAGGCACTCTCTCTGTTAACTTTGTTGATCTCTATGTTTGTATGTTTTGTGGTCGGGGAAACA ATGAAGATAAATTGCTTTTGTGTGATGGATGTGATGACAGCTATCATACATTTTGTCTAATTCCTCCACT ACCTGATGTGCCCAAAGGAGACTGGAGGTGTCCTAAATGTGTCGCCGAGGAATGTAGCAAACCTCGAGAA GCCTTTGGATTTGAACAAGCTGTACGAGAGTATACACTTCAGAGCTTTGGAGAGATGGCAGATAATTTTA AGTCTGATTATTTTAATATGCCAGTCCATATGGTTCCCACAGAACTAGTAGAAAAGGAATTTTGGCGGCT GGTAAGCAGCATTGAAGAAGATGTTATTGTGGAATATGGAGCAGATATCTCCTCAAAAGACTTTGGAAGT GGATTTCCGGTGAAGGATGGGCGGAGAAAGATTCTGCCAGAAGAAGAGGAATATGCACTTTCTGGTTGGA ATTTGAATAACATGCCTGTCCTGGAACAGTCTGTTCTTGCACATATTAATGTGGACATCTCTGGTATGAA AGTGCCGTGGCTCTATGTGGGAATGTGCTTCTCTTCTTTTTGCTGGCACATTGAGGATCACTGGAGTTAT TCCATCAACTACTTGCACTGGGGGGAGCCAAAGACATGGTATGGTGTGCCATCTCATGCTGCAGAGCAAC TGGAGGAGGTGATGAGAGAGCTGGCCCCCGAGTTATTTGAATCCCAGCCTGATCTTCTGCATCAGTTAGT TACCATCATGAACCCCAACGTGCTAATGGAGCATGGTGTGCCTGTGTACAGGACCAATCAGTGTGCTGGC GAGTTTGTTGTGACATTTCCTCGTGCCTATCACTCTGGATTTAACCAGGGCTACAACTTTGCTGAAGCTG TGAACTTCTGTACTGCTGACTGGTTGCCCATTGGACGTCAATGTGTAAATCATTACCGACGCCTAAGGCG CCACTGTGTCTTTTCACACGAGGAACTAATTTTCAAGATGGCAGCAGATCCAGAATGCTTAGATGTGGGG CTGGCTGCCATGGTCTGCAAAGAATTGACTCTCATGACTGAAGAAGAAACACGATTAAGAGAGTCTGTTG TACAGATGGGTGTCCTGATGTCAGAAGAAGAAGTGTTTGAACTTGTTCCTGATGATGAGCGGCAGTGTTC AGCATGCAGAACCACATGTTTTCTCTCTGCTCTCACATGTTCCTGTAATCCTGAGCGGCTTGTATGTCTC TACCATCCAACTGATCTGTGCCCCTGCCCCATGCAGAAGAAATGTCTTAGATATCGCTACCCATTAGAAG ACCTCCCTTCTCTGCTATATGGTGTAAAAGTCAGGGCACAGTCCTATGACACTTGGGTCAGTCGTGTTAC AGAAGCATTGTCTGCTAACTTCAACCACAAAAAAGATTTGATTGAATTGCGAGTAATGCTGGAAGATGCT GAGGATAGGAAATACCCAGAGAATGATCTCTTTCGAAAACTCAGGGATGCTGTAAAAGAAGCTGAGACCT GTGCTTCTGTGGCTCAGCTGCTTCTGAGCAAAAAGCAGAAACACAGACAGAGCCCAGATAGTGGGAGGAC TCGGACCAAACTGACAGTGGAAGAATTGAAGGCCTTTGTCCAACAACTTTTTAGTCTTCCGTGTGTCATC AGCCAAGCTCGGCAAGTAAAGAATCTGCTAGATGATGTGGAAGAGTTTCATGAACGTGCTCAGGAGGCCA TGATGGATGAAACCCCAGATTCTTCCAAACTCCAGATGTTGATAGATATGGGCTCTAGTCTCTATGTGGA ACTCCCTGAATTACCACGACTGAAGCAAGAGCTACAACAGGCTCGGTGGTTGGACGAAGTAAGACTGACC TTATCAGATCCGCAACAAGTCACTTTGGATGTCATGAAGAAGCTGATAGACTCTGGGGTAGGGTTGGCAC CCCACCATGCTGTGGAGAAAGCAATGGCTGAACTACAGGAGCTCCTTACAGTCTCTGAACGATGGGAAGA AAAGGCTAAGGTCTGCCTACAGGCAAGACCGAGGCACAGTGTGGCAAGTTTAGAAAGCATTGTGAATGAA GCCAAGAACATTCCAGCCTTTCTACCCAATGTGTTGTCCTTGAAAGAAGCCTTACAAAAGGCTCGAGAAT GGACCGCTAAAGTGGAAGCTATTCAGAGTGGCAGCAATTACGCTTATTTGGAGCAGCTTGAGAGCTTGTC TGCGAAAGGACGCCCTATTCCTGTGCGTCTTGAAGCACTGCCGCAAGTGGAATCACAGGTAGCAGCAGCA CGGGCATGGAGAGAACGGACTGGGCGGACGTTTCTTAAGAAGAATTCTAGCCATACATTGTTACAGGTGC TGAGCCCCCGGACCGACATTGGTGTATATGGGAGTGGCAAAAATAGGAGGAAAAAAGTAAAAGAACTAAT AGAAAAAGAAAAAGAAAAGGATCTGGACCTGGAGCCTCTGAGTGATCTGGAGGAAGGATTGGAGGAAACC AGAGATACAGCCATGGTGGTGGCAGTTTTCAAAGAACGGGAGCAAAAAGAGATTGAAGCCATGCATTCTC TCAGAGCAGCCAACCTAGCCAAGATGACAATGGTGGACCGCATAGAAGAAGTAAAATTTTGCATTTGCCG CAAGACAGCCAGTGGGTTTATGCTACAGTGTGAGCTCTGCAAAGACTGGTTCCATAACAGCTGTGTTCCT CTTCCTAAATCAAGTTCCCAAAAAAAAGGATCCAGCTGGCAAGCTAAAGAAGTAAAATTCCTTTGCCCTC TTTGTATGCGGTCTCGAAGGCCCAGGCTAGAGACTATTCTGTCACTCCTGGTATCCCTTCAGAAGTTGCC CGTACGGTTGCCTGAAGGAGAGGCCCTGCAGTGTTTGACAGAACGTGCTATGAGTTGGCAAGATAGAGCG CGGCAGGCTCTAGCCACAGATGAACTATCCTCTGCCCTGGCCAAACTATCTGTGTTGAGCCAGCGTATGG TGGAACAGGCGGCTCGAGAAAAAACTGAAAAGATCATCAGTGCAGAACTCCAAAAAGCAGCTGCCAATCC AGACTTACAGGGACACTTACCTAGTTTCCAGCAGTCTGCTTTTAACCGGGTGGTGAGCAGTGTGTCATCT TCTCCTCGACAAACAATGGACTATGATGATGAAGAAACAGACTCTGATGAAGACATTCGAGAGACATATG GCTACGACATGAAGGACACAGCCAGTGTGAAGTCCTCTAGTAGTCTTGAACCCAATCTTTTTTGTGATGA AGAGATTCCCATCAAATCCGAGGAGGTGGTGACCCACATGTGGACAGCACCTTCATTTTGTGCAGAGCAT GCTTATTCTTCTGCTTCTAAGAGTTGTTCTCAAGGTTCTAGCACCCCAAGGAAACAACCTCGGAAGAGCC CTTTGGTGCCCCGAAGTTTGGAACCTCCAGTGCTGGAGTTGTCACCTGGAGCTAAGGCACAACTGGAAGA ACTTATGATGGTTGGAGATCTCCTGGAAGTATCTCTGGACGAGACTCAACACATATGGCGGATTTTGCAG GCCACACACCCACCCTCTGAAGACAGATTCTTGCATATCATGGAGGATGACAGCATGGAAGAGAAACCAC TAAAAGTGAAAGGAAAGGACTCTTCAGAGAAGAAACGGAAACGGAAGCTAGAAAAGGTAGAGCAACTTTT TGGAGAAGGAAAACAGAAGTCCAAGGAGTTAAAGAAAATGGACAAACCTAGAAAGAAGAAATTAAAATTA GGTGCAGACAAATCAAAGGAGCTGAATAAACTGGCCAAGAAACTAGCAAAAGAAGAAGAGAGAAAGAAAA AGAAGGAGAAGGCTGCTGCAGCCAAAGTTGAACTTGTGAAAGAGAGCACTGAAAAGAAAAGAGAGAAAAA GGTGCTGGACATCCCCTCAAAGTATGACTGGTCAGGAGCAGAGGAGTCTGATGATGAGAATGCTGTGTGC GCAGCACAGAACTGCCAAAGGCCCTGCAAGGACAAGGTAGACTGGGTACAATGTGATGGTGGCTGTGATG AGTGGTTTCATCAAGTTTGTGTGGGTGTATCTCCAGAAATGGCTGAAAATGAAGATTACATCTGTATAAA CTGTGCAAAGAAGCAGGGGCCAGTTAGCCCAGGTCCAGCACCACCTCCTTCCTTCATAATGAGCTACAAA CTACCAATGGAGGATCTTAAAGAGACCAGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG221461 representing NM_001042603
Red=Cloning site Green=Tags(s) MAGVGPGGYAAEFVPPPECPVFEPSWEEFTDPLSFIGRIRPLAEKTGICKIRPPKDWQPPFACEVKSFRF TPRVQRLNELEAMTRVRLDFLDQLAKFWELQGSTLKIPVVERKILDLYALSKIVASKGGFEMVTKEKKWS KVGSRLGYLPGKGTGSLLKSHYERILYPYELFQSGVSLMGVQMPNLDLKEKVEPEVLSTDTQTSPEPGTR MNILPKRTRRVKTQSESGDVSRNTELKKLQIFGAGPKVVGLAMGTKDKEDEVTRRRKVTNRSDAFNMQMR QRKGTLSVNFVDLYVCMFCGRGNNEDKLLLCDGCDDSYHTFCLIPPLPDVPKGDWRCPKCVAEECSKPRE AFGFEQAVREYTLQSFGEMADNFKSDYFNMPVHMVPTELVEKEFWRLVSSIEEDVIVEYGADISSKDFGS GFPVKDGRRKILPEEEEYALSGWNLNNMPVLEQSVLAHINVDISGMKVPWLYVGMCFSSFCWHIEDHWSY SINYLHWGEPKTWYGVPSHAAEQLEEVMRELAPELFESQPDLLHQLVTIMNPNVLMEHGVPVYRTNQCAG EFVVTFPRAYHSGFNQGYNFAEAVNFCTADWLPIGRQCVNHYRRLRRHCVFSHEELIFKMAADPECLDVG LAAMVCKELTLMTEEETRLRESVVQMGVLMSEEEVFELVPDDERQCSACRTTCFLSALTCSCNPERLVCL YHPTDLCPCPMQKKCLRYRYPLEDLPSLLYGVKVRAQSYDTWVSRVTEALSANFNHKKDLIELRVMLEDA EDRKYPENDLFRKLRDAVKEAETCASVAQLLLSKKQKHRQSPDSGRTRTKLTVEELKAFVQQLFSLPCVI SQARQVKNLLDDVEEFHERAQEAMMDETPDSSKLQMLIDMGSSLYVELPELPRLKQELQQARWLDEVRLT LSDPQQVTLDVMKKLIDSGVGLAPHHAVEKAMAELQELLTVSERWEEKAKVCLQARPRHSVASLESIVNE AKNIPAFLPNVLSLKEALQKAREWTAKVEAIQSGSNYAYLEQLESLSAKGRPIPVRLEALPQVESQVAAA RAWRERTGRTFLKKNSSHTLLQVLSPRTDIGVYGSGKNRRKKVKELIEKEKEKDLDLEPLSDLEEGLEET RDTAMVVAVFKEREQKEIEAMHSLRAANLAKMTMVDRIEEVKFCICRKTASGFMLQCELCKDWFHNSCVP LPKSSSQKKGSSWQAKEVKFLCPLCMRSRRPRLETILSLLVSLQKLPVRLPEGEALQCLTERAMSWQDRA RQALATDELSSALAKLSVLSQRMVEQAAREKTEKIISAELQKAAANPDLQGHLPSFQQSAFNRVVSSVSS SPRQTMDYDDEETDSDEDIRETYGYDMKDTASVKSSSSLEPNLFCDEEIPIKSEEVVTHMWTAPSFCAEH AYSSASKSCSQGSSTPRKQPRKSPLVPRSLEPPVLELSPGAKAQLEELMMVGDLLEVSLDETQHIWRILQ ATHPPSEDRFLHIMEDDSMEEKPLKVKGKDSSEKKRKRKLEKVEQLFGEGKQKSKELKKMDKPRKKKLKL GADKSKELNKLAKKLAKEEERKKKKEKAAAAKVELVKESTEKKREKKVLDIPSKYDWSGAEESDDENAVC AAQNCQRPCKDKVDWVQCDGGCDEWFHQVCVGVSPEMAENEDYICINCAKKQGPVSPGPAPPPSFIMSYK LPMEDLKETS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001042603 |
| ORF Size | 5070 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001042603.3 |
| RefSeq Size | 10835 bp |
| RefSeq ORF | 5073 bp |
| Locus ID | 5927 |
| UniProt ID | P29375 |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a member of the Jumonji, AT-rich interactive domain 1 (JARID1) histone demethylase protein family. The encoded protein plays a role in gene regulation through the histone code by specifically demethylating lysine 4 of histone H3. The encoded protein interacts with many other proteins, including retinoblastoma protein, and is implicated in the transcriptional regulation of Hox genes and cytokines. This gene may play a role in tumor progression. [provided by RefSeq, Aug 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221461 | KDM5A (Myc-DDK-tagged)-Human lysine (K)-specific demethylase 5A (KDM5A) |
CNY 17,040.00 |
|
| RC221461L1 | Lenti ORF clone of Human lysine (K)-specific demethylase 5A (KDM5A), Myc-DDK-tagged |
CNY 18,720.00 |
|
| RC221461L3 | Lenti ORF clone of Human lysine (K)-specific demethylase 5A (KDM5A), Myc-DDK-tagged |
CNY 18,720.00 |
|
| SC311431 | KDM5A (untagged)-Human lysine (K)-specific demethylase 5A (KDM5A) |
CNY 17,048.00 |
