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BRD2 (NM_005104) Human Tagged ORF Clone
CAT＃: RG220612
- TrueORF®
BRD2 (tGFP-tagged) - Human bromodomain containing 2 (BRD2), transcript variant 1
ORF Plasmid: DDK
"NM_005104" in other vectors (6)
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CNY 9,310.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | BRD2-IT1; D6S113E; FSH; FSRG1; NAT; O27.1.1; RING3; RNF3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG220612 representing NM_005104
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACATGGGTACTATTAAGAGGAGACTTGAAAACAATTATTATTGGGCTGCTTCAGAGTGTATGCAAG ATTTTAATACCATGTTCACCAACTGTTACATTTACAACAAGCCCACTGATGATATTGTCCTAATGGCACA AACGCTGGAAAAGATATTCCTACAGAAGGTTGCATCAATGCCACAAGAAGAACAAGAGCTGGTAGTGACC ATCCCTAAGAACAGCCACAAGAAGGGGGCCAAGTTGGCAGCGCTCCAGGGCAGTGTTACCAGTGCCCATC AGGTGCCTGCCGTCTCTTCTGTGTCACACACAGCCCTCTATACTCCTCCACCTGAGATACCTACCACTGT CCTCAACATTCCCCACCCATCAGTCATTTCCTCTCCACTTCTCAAGTCCTTGCACTCTGCTGGACCCCCG CTCCTTGCTGTTACTGCAGCTCCTCCAGCCCAGCCCCTTGCCAAGAAAAAAGGCGTAAAGCGGAAAGCAG ATACTACCACCCCTACACCTACAGCCATCTTGGCTCCTGGTTCTCCAGCTAGCCCTCCTGGGAGTCTTGA GCCTAAGGCAGCACGGCTTCCCCCTATGCGTAGAGAGAGTGGTCGCCCCATCAAGCCCCCACGCAAAGAC TTGCCTGACTCTCAGCAACAACACCAGAGCTCTAAGAAAGGAAAGCTTTCAGAACAGTTAAAACATTGCA ATGGCATTTTGAAGGAGTTACTCTCTAAGAAGCATGCTGCCTATGCTTGGCCTTTCTATAAACCAGTGGA TGCTTCTGCACTTGGCCTGCATGACTACCATGACATCATTAAGCACCCCATGGACCTCAGCACTGTCAAG CGGAAGATGGAGAACCGTGATTACCGGGATGCACAGGAGTTTGCTGCTGATGTACGGCTTATGTTCTCCA ACTGCTATAAGTACAATCCCCCAGATCACGATGTTGTGGCAATGGCACGAAAGCTACAGGATGTATTTGA GTTCCGTTATGCCAAGATGCCAGATGAACCACTAGAACCAGGGCCTTTACCAGTCTCTACTGCCATGCCC CCTGGCTTGGCCAAATCGTCTTCAGAGTCCTCCAGTGAGGAAAGTAGCAGTGAGAGCTCCTCTGAGGAAG AGGAGGAGGAAGATGAGGAGGACGAGGAGGAAGAAGAGAGTGAAAGCTCAGACTCAGAGGAAGAAAGGGC TCATCGCTTAGCAGAACTACAGGAACAGCTTCGGGCAGTACATGAACAACTGGCTGCTCTGTCCCAGGGT CCAATATCCAAGCCCAAGAGGAAAAGAGAGAAAAAAGAGAAAAAGAAGAAACGGAAGGCAGAGAAGCATC GAGGCCGAGCTGGGGCCGATGAAGATGACAAGGGGCCTAGGGCACCCCGCCCACCTCAACCTAAGAAGTC CAAGAAAGCAAGTGGCAGTGGGGGTGGCAGTGCTGCTTTAGGCCCTTCTGGCTTTGGACCTTCTGGAGGA AGTGGCACCAAGCTCCCCAAAAAGGCCACAAAGACAGCCCCACCTGCCCTGCCTACAGGTTATGATTCAG AGGAGGAGGAAGAGAGCAGGCCCATGAGTTACGATGAGAAGCGGCAGCTGAGCCTGGACATCAACAAATT ACCTGGGGAGAAGCTGGGCCGAGTTGTGCATATAATCCAAGCCAGGGAGCCCTCTTTACGTGATTCAAAC CCAGAAGAGATTGAGATTGATTTTGAAACACTCAAGCCATCCACACTTAGAGAGCTTGAGCGCTATGTCC TTTCCTGCCTACGTAAGAAACCCCGGAAGCCCTACACCATTAAGAAGCCTGTGGGAAAGACAAAGGAGGA ACTGGCTTTGGAGAAAAAGCGGGAATTAGAAAAGCGGTTACAAGATGTCAGCGGACAGCTCAATTCTACT AAAAAGCCCCCCAAGAAAGCGAATGAGAAAACAGAGTCATCCTCTGCACAGCAAGTAGCAGTGTCACGCC TTAGCGCTTCCAGCTCTAGCTCAGATTCCAGCTCCTCCTCTTCCTCGTCGTCGTCTTCAGACACCAGTGA TTCAGACTCAGGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG220612 representing NM_005104
Red=Cloning site Green=Tags(s) MDMGTIKRRLENNYYWAASECMQDFNTMFTNCYIYNKPTDDIVLMAQTLEKIFLQKVASMPQEEQELVVT IPKNSHKKGAKLAALQGSVTSAHQVPAVSSVSHTALYTPPPEIPTTVLNIPHPSVISSPLLKSLHSAGPP LLAVTAAPPAQPLAKKKGVKRKADTTTPTPTAILAPGSPASPPGSLEPKAARLPPMRRESGRPIKPPRKD LPDSQQQHQSSKKGKLSEQLKHCNGILKELLSKKHAAYAWPFYKPVDASALGLHDYHDIIKHPMDLSTVK RKMENRDYRDAQEFAADVRLMFSNCYKYNPPDHDVVAMARKLQDVFEFRYAKMPDEPLEPGPLPVSTAMP PGLAKSSSESSSEESSSESSSEEEEEEDEEDEEEEESESSDSEEERAHRLAELQEQLRAVHEQLAALSQG PISKPKRKREKKEKKKKRKAEKHRGRAGADEDDKGPRAPRPPQPKKSKKASGSGGGSAALGPSGFGPSGG SGTKLPKKATKTAPPALPTGYDSEEEEESRPMSYDEKRQLSLDINKLPGEKLGRVVHIIQAREPSLRDSN PEEIEIDFETLKPSTLRELERYVLSCLRKKPRKPYTIKKPVGKTKEELALEKKRELEKRLQDVSGQLNST KKPPKKANEKTESSSAQQVAVSRLSASSSSSDSSSSSSSSSSSDTSDSDSG TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005104 |
| ORF Size | 2403 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005104.2, NP_005095.1 |
| RefSeq Size | 4693 bp |
| RefSeq ORF | 2406 bp |
| Locus ID | 6046 |
| UniProt ID | P25440 |
| Domains | BROMO |
| Protein Families | Protein Kinase |
| Gene Summary | This gene encodes a transcriptional regulator that belongs to the BET (bromodomains and extra terminal domain) family of proteins. This protein associates with transcription complexes and with acetylated chromatin during mitosis, and it selectively binds to the acetylated lysine-12 residue of histone H4 via its two bromodomains. The gene maps to the major histocompatability complex (MHC) class II region on chromosome 6p21.3, but sequence comparison suggests that the protein is not involved in the immune response. This gene has been implicated in juvenile myoclonic epilepsy, a common form of epilepsy that becomes apparent in adolescence. Multiple alternatively spliced variants have been described for this gene. [provided by RefSeq, Dec 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220612 | BRD2 (Myc-DDK-tagged)-Human bromodomain containing 2 (BRD2), transcript variant 1 |
CNY 7,480.00 |
|
| RC220612L1 | Lenti ORF clone of Human bromodomain containing 2 (BRD2), transcript variant 1, Myc-DDK-tagged |
CNY 9,880.00 |
|
| RC220612L2 | Lenti ORF clone of Human bromodomain containing 2 (BRD2), transcript variant 1, mGFP tagged |
CNY 10,360.00 |
|
| RC220612L3 | Lenti ORF clone of Human bromodomain containing 2 (BRD2), transcript variant 1, Myc-DDK-tagged |
CNY 10,360.00 |
|
| RC220612L4 | Lenti ORF clone of Human bromodomain containing 2 (BRD2), transcript variant 1, mGFP tagged |
CNY 9,880.00 |
|
| SC116928 | BRD2 (untagged)-Human bromodomain containing 2 (BRD2), transcript variant 1 |
CNY 15,390.00 |
