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DROSHA (NM_001100412) Human Tagged ORF Clone
CAT＃: RG217782
- TrueORF®
DROSHA (tGFP-tagged) - Human drosha, ribonuclease type III (DROSHA), transcript variant 2
ORF Plasmid: DDK
"NM_001100412" in other vectors (4)
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CNY 14,630.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ETOHI2; HSA242976; RANSE3L; RN3; RNASE3L; RNASEN |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG217782 representing NM_001100412
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGCAGGGAAACACATGTCACAGAATGTCGTTCCACCCGGGACGAGGGTGTCCCCGAGGACGAGGAG GACATGGAGCCAGACCCTCAGCACCATCCTTTAGGCCCCAAAATCTGAGGCTGCTTCACCCTCAGCAGCC TCCTGTGCAATATCAATATGAACCTCCAAGTGCCCCTTCCACCACTTTCTCAAACTCTCCAGCCCCCAAT TTTCTCCCTCCACGACCAGACTTTGTACCCTTCCCCCCACCCATGCCTCCGTCAGCGCAAGGCCCTCTTC CCCCCTGCCCAATCAGGCCGCCTTTCCCCAACCACCAGATGAGGCACCCCTTCCCAGTTCCTCCTTGTTT TCCTCCCATGCCACCACCAATGCCTTGTCCTAATAACCCCCCAGTCCCTGGGGCACCTCCTGGACAAGGC ACTTTCCCCTTCATGATGCCCCCTCCCTCCATGCCTCATCCCCCGCCCCCTCCAGTCATGCCGCAGCAGG TTAATTATCAGTACCCTCCGGGCTATTCTCACCACAACTTCCCACCTCCCAGTTTTAATAGTTTCCAGAA CAACCCTAGTTCTTTCCTGCCCAGTGCTAATAACAGCAGTAGTCCTCATTTCAGACATCTCCCTCCATAC CCACTCCCAAAGGCTCCCAGTGAGAGAAGGTCCCCAGAAAGGCTGAAACACTATGATGACCACAGGCACC GAGATCACAGTCATGGGCGAGGTGAGAGGCATCGGTCCCTGGATCGGCGGGAGCGAGGCCGCAGTCCCGA CAGGAGAAGACAAGACAGCCGGTACAGATCTGATTATGACCGAGGGAGAACACCATCTCGCCACCGCAGC TACGAACGGAGCAGAGAGCGAGAACGGGAGAGACACAGGCATCGAGACAACCGAAGATCACCATCTCTGG AAAGGTCCTACAAAAAAGAGTATAAGAGATCTGGAAGTCGCTCCCCAAGTAGGGAGAAGAAGAGAGCTCG TTGGGAGGAAGAAAAAGACCGTTGGAGTGACAACCAGAGTTCTGGCAAAGACAAGAACTATACCTCAATC AAGGAAAAAGAGCCCGAGGAGACCATGCCTGACAAGAATGAGGAGGAAGAAGAAGAACTTCTTAAGCCTG TGTGGATTCGATGCACTCATTCAGAAAACTACTACTCCAGTGACCCCATGGATCAGGTGGGAGATTCTAC AGTGGTTGGAACGAGTAGGCTTCGTGACTTATATGACAAATTTGAGGAGGAGTTGGGGAGCAGGCAAGAA AAGGCCAAAGCTGCTCGGCCTCCGTGGGAACCTCCAAAGACGAAGCTCGATGAAGATTTAGAGAGTTCCA GTGAATCCGAGTGTGAGTCTGATGAGGACAGCACCTGTTCTAGCAGCTCAGACTCTGAAGTTTTTGACGT TATTGCAGAAATCAAACGCAAAAAGGCCCACCCTGACCGACTTCATGATGAACTTTGGTACAACGATCCA GGCCAGATGAATGATGGACCACTCTGCAAATGCAGCGCAAAGGCAAGACGCACAGGAATTAGGCACAGCA TTTATCCTGGAGAAGAGGCCATCAAGCCCTGTCGTCCTATGACCAACAATGCTGGCAGACTTTTCCACTA CCGGATCACAGTCTCCCCGCCTACGAACTTTTTAACTGACAGGCCAACTGTTATAGAATACGATGATCAC GAGTATATCTTTGAAGGATTTTCTATGTTTGCACATGCCCCCCTGACCAATATTCCACTGTGTAAAGTAA TTAGATTCAACATAGACTACACGATTCATTTCATTGAAGAGATGATGCCGGAGAATTTTTGTGTGAAAGG GCTTGAACTCTTTTCACTGTTCCTATTCAGAGATATTTTGGAATTATATGACTGGAATCTTAAAGGTCCT TTGTTTGAAGACAGCCCTCCCTGCTGCCCAAGATTTCATTTCATGCCACGTTTTGTAAGATTTCTTCCAG ATGGAGGAAAGGAAGTGCTGTCCATGCACCAGATTCTCCTGTACTTGTTAAGGTGCAGCAAAGCCCTGGT GCCTGAGGAGGAGATTGCCAATATGCTTCAGTGGGAGGAGCTGGAGTGGCAGAAATATGCAGAAGAATGC AAAGGCATGATTGTTACCAACCCTGGGACGAAACCAAGCTCTGTCCGTATCGATCAACTGGATCGTGAAC AGTTCAACCCCGATGTGATTACTTTTCCGATTATCGTCCACTTTGGGATACGCCCTGCACAGTTGAGTTA TGCAGGAGACCCACAGTACCAAAAACTGTGGAAGAGTTATGTGAAACTTCGCCACCTCCTAGCAAATAGT CCCAAAGTCAAACAAACTGACAAACAGAAGCTGGCACAGAGGGAGGAAGCCCTCCAAAAAATACGGCAGA AGAATACAATGAGACGAGAAGTAACGGTGGAGCTAAGTAGCCAAGGATTCTGGAAAACTGGCATCCGTTC TGATGTCTGTCAGCATGCAATGATGCTACCTGTTCTGACCCATCATATCCGCTACCACCAATGCCTAATG CATTTGGACAAGTTGATAGGATATACTTTCCAAGATCGTTGTCTGTTGCAGCTGGCCATGACTCATCCAA GTCATCATTTAAATTTTGGAATGAATCCTGATCATGCCAGGAATTCATTATCTAACTGTGGAATTCGGCA GCCCAAATACGGAGACAGAAAAGTTCATCACATGCACATGCGGAAGAAAGGGATTAACACCTTGATAAAT ATCATGTCACGCCTTGGCCAAGATGACCCAACTCCCTCGAGGATTAACCACAATGAACGGTTGGAATTCC TGGGTGATGCTGTTGTTGAATTTCTGACCAGCGTCCATTTGTACTATTTGTTTCCTAGTCTGGAAGAAGG AGGATTAGCAACCTATCGGACTGCCATTGTTCAGAATCAGCACCTTGCCATGCTAGCAAAGAAACTTGAA CTGGATCGATTTATGCTGTATGCTCACGGGCCTGACCTTTGTAGAGAATCGGACCTTCGACATGCAATGG CCAATTGTTTTGAAGCGTTAATAGGAGCTGTTTACTTGGAGGGAAGCCTGGAGGAAGCCAAGCAGTTATT TGGACGCTTGCTCTTTAATGATCCGGACCTGCGCGAAGTCTGGCTCAATTATCCTCTCCACCCACTCCAA CTACAAGAGCCAAATACTGATCGACAACTTATTGAAACTTCTCCAGTTCTACAAAAACTTACTGAGTTTG AAGAAGCAATTGGAGTAATTTTTACTCATGTTCGACTTCTGGCAAGGGCATTCACATTGAGAACTGTGGG ATTTAACCATCTGACCCTAGGCCACAATCAGAGAATGGAATTCCTAGGTGACTCCATAATGCAACTGGTA GCCACAGAGTACTTATTCATTCATTTCCCAGATCATCATGAAGGACACTTAACTTTGTTGCGAAGCTCTT TGGTGAATAATAGAACTCAGGCCAAGGTAGCGGAGGAGCTGGGCATGCAGGAGTACGCCATAACCAACGA CAAGACCAAGAGGCCTGTGGCGCTTCGCACCAAGACCTTGGCGGACCTTTTGGAATCATTTATTGCAGCG CTGTACATTGATAAGGATTTGGAATATGTTCATACTTTCATGAATGTCTGCTTCTTTCCACGATTGAAAG AGTTCATTTTGAATCAGGATTGGAATGACCCCAAATCCCAGCTTCAGCAGTGTTGCTTGACACTTAGGAC AGAAGGAAAAGAGCCAGACATTCCTCTGTACAAGACTCTGCAGACAGTGGGCCCATCCCATGCCCGAACC TACACTGTGGCTGTTTATTTCAAGGGAGAAAGAATAGGCTGTGGGAAAGGACCAAGTATTCAGCAAGCGG AAATGGGAGCAGCAATGGATGCGCTTGAAAAATATAATTTTCCCCAGATGGCCCATCAGAAGCGGTTCAT CGAACGGAAGTACAGACAAGAGTTAAAAGAAATGAGGTGGGAAAGAGAGCATCAAGAGAGAGAGCCAGAT GAGACTGAAGACATCAAGAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG217782 representing NM_001100412
Red=Cloning site Green=Tags(s) MMQGNTCHRMSFHPGRGCPRGRGGHGARPSAPSFRPQNLRLLHPQQPPVQYQYEPPSAPSTTFSNSPAPN FLPPRPDFVPFPPPMPPSAQGPLPPCPIRPPFPNHQMRHPFPVPPCFPPMPPPMPCPNNPPVPGAPPGQG TFPFMMPPPSMPHPPPPPVMPQQVNYQYPPGYSHHNFPPPSFNSFQNNPSSFLPSANNSSSPHFRHLPPY PLPKAPSERRSPERLKHYDDHRHRDHSHGRGERHRSLDRRERGRSPDRRRQDSRYRSDYDRGRTPSRHRS YERSRERERERHRHRDNRRSPSLERSYKKEYKRSGSRSPSREKKRARWEEEKDRWSDNQSSGKDKNYTSI KEKEPEETMPDKNEEEEEELLKPVWIRCTHSENYYSSDPMDQVGDSTVVGTSRLRDLYDKFEEELGSRQE KAKAARPPWEPPKTKLDEDLESSSESECESDEDSTCSSSSDSEVFDVIAEIKRKKAHPDRLHDELWYNDP GQMNDGPLCKCSAKARRTGIRHSIYPGEEAIKPCRPMTNNAGRLFHYRITVSPPTNFLTDRPTVIEYDDH EYIFEGFSMFAHAPLTNIPLCKVIRFNIDYTIHFIEEMMPENFCVKGLELFSLFLFRDILELYDWNLKGP LFEDSPPCCPRFHFMPRFVRFLPDGGKEVLSMHQILLYLLRCSKALVPEEEIANMLQWEELEWQKYAEEC KGMIVTNPGTKPSSVRIDQLDREQFNPDVITFPIIVHFGIRPAQLSYAGDPQYQKLWKSYVKLRHLLANS PKVKQTDKQKLAQREEALQKIRQKNTMRREVTVELSSQGFWKTGIRSDVCQHAMMLPVLTHHIRYHQCLM HLDKLIGYTFQDRCLLQLAMTHPSHHLNFGMNPDHARNSLSNCGIRQPKYGDRKVHHMHMRKKGINTLIN IMSRLGQDDPTPSRINHNERLEFLGDAVVEFLTSVHLYYLFPSLEEGGLATYRTAIVQNQHLAMLAKKLE LDRFMLYAHGPDLCRESDLRHAMANCFEALIGAVYLEGSLEEAKQLFGRLLFNDPDLREVWLNYPLHPLQ LQEPNTDRQLIETSPVLQKLTEFEEAIGVIFTHVRLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLV ATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKVAEELGMQEYAITNDKTKRPVALRTKTLADLLESFIAA LYIDKDLEYVHTFMNVCFFPRLKEFILNQDWNDPKSQLQQCCLTLRTEGKEPDIPLYKTLQTVGPSHART YTVAVYFKGERIGCGKGPSIQQAEMGAAMDALEKYNFPQMAHQKRFIERKYRQELKEMRWEREHQEREPD ETEDIKK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001100412 |
| ORF Size | 4011 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001100412.2 |
| RefSeq Size | 5389 bp |
| RefSeq ORF | 4014 bp |
| Locus ID | 29102 |
| UniProt ID | Q9NRR4 |
| Gene Summary | This gene encodes a ribonuclease (RNase) III double-stranded RNA-specific ribonuclease and subunit of the microprocessor protein complex, which catalyzes the initial processing step of microRNA (miRNA) synthesis. The encoded protein cleaves the stem loop structure from the primary microRNA (pri-miRNA) in the nucleus, yielding the precursor miRNA (pre-miRNA), which is then exported to the cytoplasm for further processing. In a human cell line lacking a functional copy of this gene, canonical miRNA synthesis is reduced. Somatic mutations in this gene have been observed in human patients with kidney cancer. [provided by RefSeq, Sep 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217782 | DROSHA (Myc-DDK-tagged)-Human drosha, ribonuclease type III (DROSHA), transcript variant 2 |
CNY 13,300.00 |
|
| RC217782L3 | Lenti-ORF clone of DROSHA (Myc-DDK-tagged)-Human drosha, ribonuclease type III (DROSHA), transcript variant 2 |
CNY 15,200.00 |
|
| RC217782L4 | Lenti-ORF clone of DROSHA (mGFP-tagged)-Human drosha, ribonuclease type III (DROSHA), transcript variant 2 |
CNY 15,200.00 |
|
| SC316870 | DROSHA (untagged)-Human drosha, ribonuclease type III (DROSHA), transcript variant 2 |
CNY 25,750.00 |
