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USP33 (NM_201624) Human Tagged ORF Clone
CAT＃: RG217069
- TrueORF®
USP33 (tGFP-tagged) - Human ubiquitin specific peptidase 33 (USP33), transcript variant 2
ORF Plasmid: DDK
"NM_201624" in other vectors (4)
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CNY 7,890.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | VDU1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG217069 representing NM_201624
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAGCTTTTCGAAATCATTGTCCACATTTGGATTCAGTTGGTGAAATAACAAAAGAAGATTTGATAC AAAAATCCCTTGGTACTTGTCAGGATTGTAAAGTCCAAGGACCAAATCTTTGGGCATGTCTGGAGAATAG ATGTTCATATGTTGGCTGTGGTGAATCACAAGTAGATCACAGCACCATACATTCTCAGGAGACAAAGCAT TATCTAACTGTGAACCTTACCACTCTTCGAGTATGGTGTTATGCTTGCAGCAAAGAAGTATTTTTGGATA GGAAATTAGGAACTCAGCCTTCATTGCCTCATGTAAGACAACCTCACCAAATACAAGAAAACAGTGTCCA GGATTTTAAAATACCCAGTAATACAACATTAAAAACTCCTCTGGTTGCCGTATTTGATGATCTGGATATA GAAGCGGATGAAGAAGATGAACTTAGGGCCAGAGGTCTTACAGGTTTGAAAAATATTGGAAATACTTGTT ACATGAATGCAGCTTTGCAGGCTCTTTCTAATTGCCCACCTTTGACACAGTTTTTTCTTGATTGTGGAGG ACTAGCTCGAACAGATAAGAAACCTGCCATTTGTAAAAGTTATCTCAAACTAATGACAGAGCTGTGGCAT AAAAGCAGGCCAGGATCTGTTGTGCCTACTACTCTGTTTCAAGGAATTAAAACTGTAAATCCAACATTTC GGGGGTATTCTCAGCAGGATGCTCAAGAATTCCTTCGATGTTTAATGGATTTGCTTCATGAAGAATTGAA AGAGCAAGTCATGGAAGTAGAAGAAGATCCGCAAACCATAACCACTGAGGAGACAATGGAAGAAGACAAG AGCCAGTCGGATGTAGATTTTCAGTCTTGTGAATCTTGTAGCAACAGTGATAGAGCAGAAAATGAAAATG GCTCTAGATGCTTTTCTGAAGATAATAATGAAACAACAATGTTAATTCAGGATGATGAAAACAATTCAGA AATGTCAAAGGATTGGCAAAAAGAGAAGATGTGCAATAAGATTAATAAAGTAAATTCTGAAGGCGAATTT GATAAAGATAGAGACTCTATATCTGAAACAGTCGACTTAAACAACCAGGAAACTGTCAAAGTGCAAATAC ACAGCAGAGCTTCAGAATATATCACTGATGTCCATTCGAATGACCTGTCTACACCACAGATCCTTCCATC AAATGAAGGTGTTAATCCACGTTTATCGGCAAGCCCTCCTAAATCAGGCAATTTGTGGCCAGGATTGGCA CCACCACACAAAAAAGCTCAGTCTGCATCTCCAAAGAGAAAAAAACAGCACAAGAAATACAGAAGTGTTA TTTCAGACATATTTGATGGAACAATCATTAGTTCAGTGCAGTGTCTGACTTGTGACAGGGTGTCTGTAAC CCTCGAGACCTTTCAAGATCTGTCCTTGCCAATTCCTGGCAAGGAAGACCTTGCTAAGCTGCATTCATCA AGTCATCCAACTTCTATAGTCAAAGCAGGATCATGTGGCGAAGCATATGCTCCACAAGGGTGGATAGCTT TTTTCATGGAATATGTGAAGAGGTTTGTTGTCTCATGTGTCCCTAGCTGGTTTTGGGGTCCAGTAGTAAC CTTGCAAGATTGTCTTGCTGCCTTCTTTGCCAGAGATGAACTAAAAGGTGACAATATGTACAGTTGTGAA AAATGCAAAAAGTTGAGAAATGGAGTGAAGTTTTGTAAAGTACAAAACTTTCCTGAGATTTTGTGCATCC ACCTTAAAAGATTCAGACATGAACTAATGTTTTCCACCAAAATCAGTACCCATGTTTCATTTCCGCTAGA AGGCTTGGATCTTCAGCCATTTCTTGCTAAGGATAGTCCAGCTCAAATTGTGACATATGATCTTCTGTCA GTCATTTGCCATCATGGAACTGCAAGTAGTGGACACTATATAGCCTACTGCCGAAACAATCTAAATAATC TCTGGTATGAATTTGATGATCAGAGTGTCACTGAAGTTTCAGAATCTACTGTACAAAATGCAGAAGCTTA CGTTCTTTTCTATAGGAAGAGCAGCGAAGAGGCACAAAAAGAGAGGAGAAGGATATCAAATTTATTGAAC ATAATGGAACCAAGCCTCCTTCAGTTTTATATTTCTCGACAGTGGCTTAATAAATTTAAGACCTTTGCCG AACCTGGCCCTATTTCAAATAATGACTTTCTTTGTATTCATGGAGGTGTTCCTCCAAGAAAAGCTGGTTA TATTGAAGACCTGGTTTTGATGCTGCCTCAGAACATTTGGGATAACCTATATAGCAGGTATGGTGGAGGA CCAGCTGTCAACCATCTGTACATTTGTCATACTTGCCAAATTGAGGCGGAGAAAATTGAAAAAAGAAGAA AAACTGAATTGGAAATTTTTATTCGGCTTAACAGAGCGTTCCAAAAAGAGGACTCTCCAGCTACTTTTTA TTGCATCAGTATGCAGTGGTTTAGAGAATGGGAAAGTTTTGTGAAGGGTAAAGATGGAGATCCTCCAGGT CCTATTGACAATACTAAGATTGCAGTCACTAAATGTGGTAATGTGATGCTTAGGCAAGGAGCAGATTCTG GCCAGATTTCTGAAGAAACATGGAATTTTCTGCAGTCTATTTATGGTGGAGGGCCTGAAGTTATCCTGCG ACCTCCGGTTGTTCATGTTGATCCAGATATACTTCAAGCAGAAGAAAAAATTGAAGTAGAAACTCGGTCT TTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG217069 representing NM_201624
Red=Cloning site Green=Tags(s) MSAFRNHCPHLDSVGEITKEDLIQKSLGTCQDCKVQGPNLWACLENRCSYVGCGESQVDHSTIHSQETKH YLTVNLTTLRVWCYACSKEVFLDRKLGTQPSLPHVRQPHQIQENSVQDFKIPSNTTLKTPLVAVFDDLDI EADEEDELRARGLTGLKNIGNTCYMNAALQALSNCPPLTQFFLDCGGLARTDKKPAICKSYLKLMTELWH KSRPGSVVPTTLFQGIKTVNPTFRGYSQQDAQEFLRCLMDLLHEELKEQVMEVEEDPQTITTEETMEEDK SQSDVDFQSCESCSNSDRAENENGSRCFSEDNNETTMLIQDDENNSEMSKDWQKEKMCNKINKVNSEGEF DKDRDSISETVDLNNQETVKVQIHSRASEYITDVHSNDLSTPQILPSNEGVNPRLSASPPKSGNLWPGLA PPHKKAQSASPKRKKQHKKYRSVISDIFDGTIISSVQCLTCDRVSVTLETFQDLSLPIPGKEDLAKLHSS SHPTSIVKAGSCGEAYAPQGWIAFFMEYVKRFVVSCVPSWFWGPVVTLQDCLAAFFARDELKGDNMYSCE KCKKLRNGVKFCKVQNFPEILCIHLKRFRHELMFSTKISTHVSFPLEGLDLQPFLAKDSPAQIVTYDLLS VICHHGTASSGHYIAYCRNNLNNLWYEFDDQSVTEVSESTVQNAEAYVLFYRKSSEEAQKERRRISNLLN IMEPSLLQFYISRQWLNKFKTFAEPGPISNNDFLCIHGGVPPRKAGYIEDLVLMLPQNIWDNLYSRYGGG PAVNHLYICHTCQIEAEKIEKRRKTELEIFIRLNRAFQKEDSPATFYCISMQWFREWESFVKGKDGDPPG PIDNTKIAVTKCGNVMLRQGADSGQISEETWNFLQSIYGGGPEVILRPPVVHVDPDILQAEEKIEVETRS L TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_201624 |
| ORF Size | 2733 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_201624.3 |
| RefSeq Size | 4345 bp |
| RefSeq ORF | 2736 bp |
| Locus ID | 23032 |
| UniProt ID | Q8TEY7 |
| Protein Families | Druggable Genome, Protease |
| Gene Summary | This gene encodes a deubiquinating enzyme important in a variety of processes, including Slit-dependent cell migration and beta-2 adrenergic receptor signaling. The protein is negatively regulated through ubiquitination by von Hippel-Lindau tumor protein (VHL). Alternative splicing results in multiple transcript variants and protein isoforms. [provided by RefSeq, Jun 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217069 | USP33 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 33 (USP33), transcript variant 2 |
CNY 6,672.00 |
|
| RC217069L3 | Lenti ORF clone of Human ubiquitin specific peptidase 33 (USP33), transcript variant 2, Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC217069L4 | Lenti ORF clone of Human ubiquitin specific peptidase 33 (USP33), transcript variant 2, mGFP tagged |
CNY 9,030.00 |
|
| SC314904 | USP33 (untagged)-Human ubiquitin specific peptidase 33 (USP33), transcript variant 2 |
CNY 17,480.00 |
