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HAT1 (NM_003642) Human Tagged ORF Clone
CAT＃: RG213094
- TrueORF®
HAT1 (tGFP-tagged) - Human histone acetyltransferase 1 (HAT1), transcript variant 1. Note: ORF is codon optimized
ORF Plasmid: DDK
"NM_003642" in other vectors (2)
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CNY 5,586.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | KAT1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG213094 ORF sequence, codon optimized.
Due to the complexity of NM_003642, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGCTTCGGCGCTATGGAGAAATTTCTCGTTGAATACAAGTCTGCCGTGGAGAAGAAATTGGCGG AGTACAAGTGCAATACCAACACTGCTATTGAACTCAAATTGGTTCGCTTCCCCGAAGACCTCGAAAATGA CATCAGGACATTTTTTCCAGAATATACTCATCAGCTGTTCGGGGACGATGAAACAGCCTTTGGTTATAAA GGCCTCAAAATTCTGCTCTACTACATTGCAGGAAGCTTGAGCACCATGTTCCGCGTAGAATATGCGAGTA AGGTGGATGAGAATTTCGACTGCGTCGAGGCCGACGATGTGGAAGGGAAGATACGGCAGATCATCCCTCC CGGCTTTTGTACTAATACCAATGACTTTCTTTCCCTGCTCGAAAAGGAGGTGGACTTCAAGCCTTTCGGC ACCCTGCTGCATACCTACAGTGTGCTTTCTCCTACTGGCGGGGAAAACTTCACGTTCCAAATCTACAAGG CCGACATGACTTGTAGAGGGTTTCGGGAGTACCATGAGAGGTTGCAGACTTTTCTCATGTGGTTCATCGA AACGGCCTCATTCATCGACGTGGATGATGAGCGCTGGCACTACTTTCTGGTATTTGAAAAGTACAATAAG GACGGCGCCACATTGTTTGCGACTGTGGGGTACATGACCGTGTACAATTACTACGTTTATCCGGACAAGA CACGCCCACGAGTAAGTCAGATGCTGATCCTTACCCCTTTCCAAGGGCAGGGACACGGCGCGCAATTGCT TGAAACTGTCCACCGCTACTATACCGAGTTCCCCACTGTGCTCGATATTACAGCTGAGGACCCCTCCAAG TCCTACGTGAAGCTGCGAGATTTTGTGTTGGTGAAACTGTGCCAAGATCTCCCCTGTTTCTCCCGAGAAA AGCTGATGCAGGGATTTAACGAGGACATGGCCATCGAAGCCCAGCAGAAGTTTAAAATTAACAAGCAGCA CGCTAGGCGAGTTTATGAAATTTTGAGGCTCTTGGTCACAGATATGAGCGACGCCGAACAGTATAGGTCT TACCGGCTCGACATAAAACGGCGGCTGATATCACCTTATAAGAAGAAACAGCGAGATCTGGCAAAAATGA GAAAATGTCTGAGGCCCGAGGAGCTTACTAACCAAATGAATCAGATCGAGATTTCAATGCAACATGAACA ACTGGAGGAGTCATTCCAAGAACTGGTCGAGGATTACAGGAGAGTCATCGAGCGGCTCGCCCAGGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG213094 representing NM_003642
Red=Cloning site Green=Tags(s) MAGFGAMEKFLVEYKSAVEKKLAEYKCNTNTAIELKLVRFPEDLENDIRTFFPEYTHQLFGDDETAFGYK GLKILLYYIAGSLSTMFRVEYASKVDENFDCVEADDVEGKIRQIIPPGFCTNTNDFLSLLEKEVDFKPFG TLLHTYSVLSPTGGENFTFQIYKADMTCRGFREYHERLQTFLMWFIETASFIDVDDERWHYFLVFEKYNK DGATLFATVGYMTVYNYYVYPDKTRPRVSQMLILTPFQGQGHGAQLLETVHRYYTEFPTVLDITAEDPSK SYVKLRDFVLVKLCQDLPCFSREKLMQGFNEDMAIEAQQKFKINKQHARRVYEILRLLVTDMSDAEQYRS YRLDIKRRLISPYKKKQRDLAKMRKCLRPEELTNQMNQIEISMQHEQLEESFQELVEDYRRVIERLAQE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003642 |
| ORF Size | 1257 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003642.2, NP_003633.1 |
| RefSeq Size | 1634 bp |
| RefSeq ORF | 1260 bp |
| Locus ID | 8520 |
| UniProt ID | O14929 |
| Protein Families | Druggable Genome |
| Gene Summary | The protein encoded by this gene is a type B histone acetyltransferase (HAT) that is involved in the rapid acetylation of newly synthesized cytoplasmic histones, which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains. Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. Specifically, this HAT can acetylate soluble but not nucleosomal histone H4 at lysines 5 and 12, and to a lesser degree, histone H2A at lysine 5. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jun 2009] |
Documents
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