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RBM5 (NM_005778) Human Tagged ORF Clone
CAT＃: RG212543
- TrueORF®
RBM5 (tGFP-tagged) - Human RNA binding motif protein 5 (RBM5), transcript variant 1
ORF Plasmid: DDK
"NM_005778" in other vectors (6)
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CNY 7,568.00
CNY 9,500.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | G15; H37; LUCA-15; LUCA15; RMB5 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG212543 representing NM_005778
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTTCAGACAAAAGAGTGAGTAGAACAGAGCGTAGTGGAAGATACGGTTCCATCATAGACAGGGATG ACCGTGATGAGCGTGAATCCCGAAGCAGGCGGAGGGACTCAGATTACAAAAGATCTAGTGATGATCGGAG GGGTGATAGATATGATGACTACCGAGACTATGACAGTCCAGAGAGAGAGCGTGAAAGAAGGAACAGTGAC CGATCCGAAGATGGCTACCATTCAGATGGTGACTATGGTGAGCACGACTATAGGCATGACATCAGTGACG AGAGGGAGAGCAAGACCATCATGCTGCGCGGCCTTCCCATCACCATCACAGAGAGCGATATTCGAGAAAT GATGGAGTCCTTCGAAGGCCCTCAGCCTGCGGATGTGAGGCTGATGAAGAGGAAAACAGGTGTAAGCCGT GGTTTCGCCTTCGTGGAGTTTTATCACTTGCAAGATGCTACCAGCTGGATGGAAGCCAATCAGAAAAAGT TGGTGATTCAAGGAAAGCACATTGCAATGCATTATAGCAATCCCAGACCTAAGTTTGAAGATTGGCTTTG TAACAAGTGCTGCCTTAACAATTTCAGGAAAAGACTAAAATGCTTCCGATGTGGAGCAGACAAGTTTGAC TCTGAACAGGAAGTGCCTCCTGGAACCACAGAGTCGGTTCAGTCTGTGGATTACTACTGTGATACGATCA TTCTTCGGAACATAGCTCCGCACACTGTGGTGGATTCCATCATGACAGCACTGTCTCCTTACGCGTCTTT AGCTGTCAATAACATCCGCCTCATAAAAGACAAACAGACCCAGCAGAACAGAGGCTTCGCATTTGTGCAG CTGTCCTCTGCAATGGATGCTTCTCAGCTGCTTCAGATATTACAGAGTCTCCATCCTCCTTTGAAAATTG ATGGCAAAACTATTGGGGTTGATTTTGCAAAAAGTGCCAGAAAAGACTTGGTCCTCTCAGATGGTAACCG CGTCAGCGCCTTCTCTGTAGCTAGTACGGCTATTGCTGCTGCTCAGTGGTCATCCACCCAGTCTCAAAGT GGTGAAGGAGGCAGTGTTGACTACAGTTATCTGCAACCAGGTCAAGATGGCTATGCCCAATATGCTCAGT ATTCACAGGATTATCAGCAGTTTTATCAACAACAAGCTGGAGGATTGGAATCTGATGCATCATCTGCATC AGGCACAGCAGTGACCACCACCTCAGCGGCTGTAGTGTCCCAGAGTCCTCAGCTGTATAATCAAACCTCC AATCCACCTGGCTCTCCGACTGAGGAAGCACAGCCTAGCACTAGCACAAGTACACAGGCCCCAGCCGCTT CCCCTACTGGTGTAGTTCCTGGTACCAAATATGCAGTACCTGACACGTCCACTTACCAGTATGATGAATC TTCAGGATATTACTATGATCCGACAACAGGGCTCTATTATGACCCCAACTCGCAATACTACTATAATTCC TTGACCCAGCAGTACCTTTACTGGGATGGGGAAAAAGAGACCTACGTGCCAGCTGCAGAGTCTAGCTCCC ACCAGCAGTCGGGCCTGCCTCCTGCAAAAGAGGGGAAAGAGAAGAAGGAGAAACCCAAGAGCAAAACAGC CCAGCAGATTGCCAAAGACATGGAACGCTGGGCTAAGAGTTTGAATAAGCAGAAAGAAAACTTTAAAAAT AGCTTTCAGCCTGTCAATTCCTTGAGGGAAGAAGAAAGGAGAGAATCTGCTGCAGCAGACGCTGGCTTTG CTCTCTTTGAGAAGAAGGGAGCCTTAGCTGAAAGGCAGCAGCTCATCCCAGAATTGGTGCGAAATGGAGA TGAGGAGAATCCCCTCAAAAGGGGTCTGGTTGCTGCTTACAGTGGTGACAGTGACAATGAGGAGGAGCTG GTGGAGAGACTTGAGAGTGAGGAAGAGAAGCTAGCTGACTGGAAGAAGATGGCCTGTCTGCTCTGCCGGC GCCAGTTCCCGAACAAAGATGCCCTAGTCAGGCACCAGCAACTCTCAGACCTTCACAAGCAAAACATGGA CATCTACCGACGATCCAGGCTGAGCGAGCAGGAGCTGGAAGCCTTGGAGCTAAGGGAGAGAGAGATGAAA TACCGAGACCGAGCTGCAGAAAGACGGGAGAAGTACGGCATTCCAGAACCTCCAGAGCCCAAGCGCAAGA AGCAGTTTGATGCCGGCACTGTGAATTACGAGCAACCCACCAAAGATGGCATTGACCACAGTAACATTGG CAACAAGATGCTGCAGGCCATGGGCTGGCGGGAAGGCTCTGGCTTGGGACGAAAGTGTCAAGGCATTACG GCTCCCATTGAGGCTCAAGTTCGGCTAAAGGGAGCTGGCCTAGGAGCCAAAGGCAGCGCATATGGTTTGT CGGGCGCCGATTCCTACAAAGATGCTGTCCGGAAAGCCATGTTTGCCCGGTTCACTGAGATGGAG AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG212543 representing NM_005778
Red=Cloning site Green=Tags(s) MGSDKRVSRTERSGRYGSIIDRDDRDERESRSRRRDSDYKRSSDDRRGDRYDDYRDYDSPERERERRNSD RSEDGYHSDGDYGEHDYRHDISDERESKTIMLRGLPITITESDIREMMESFEGPQPADVRLMKRKTGVSR GFAFVEFYHLQDATSWMEANQKKLVIQGKHIAMHYSNPRPKFEDWLCNKCCLNNFRKRLKCFRCGADKFD SEQEVPPGTTESVQSVDYYCDTIILRNIAPHTVVDSIMTALSPYASLAVNNIRLIKDKQTQQNRGFAFVQ LSSAMDASQLLQILQSLHPPLKIDGKTIGVDFAKSARKDLVLSDGNRVSAFSVASTAIAAAQWSSTQSQS GEGGSVDYSYLQPGQDGYAQYAQYSQDYQQFYQQQAGGLESDASSASGTAVTTTSAAVVSQSPQLYNQTS NPPGSPTEEAQPSTSTSTQAPAASPTGVVPGTKYAVPDTSTYQYDESSGYYYDPTTGLYYDPNSQYYYNS LTQQYLYWDGEKETYVPAAESSSHQQSGLPPAKEGKEKKEKPKSKTAQQIAKDMERWAKSLNKQKENFKN SFQPVNSLREEERRESAAADAGFALFEKKGALAERQQLIPELVRNGDEENPLKRGLVAAYSGDSDNEEEL VERLESEEEKLADWKKMACLLCRRQFPNKDALVRHQQLSDLHKQNMDIYRRSRLSEQELEALELREREMK YRDRAAERREKYGIPEPPEPKRKKQFDAGTVNYEQPTKDGIDHSNIGNKMLQAMGWREGSGLGRKCQGIT APIEAQVRLKGAGLGAKGSAYGLSGADSYKDAVRKAMFARFTEME SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005778 |
| ORF Size | 2445 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005778.1, NP_005769.1 |
| RefSeq Size | 3135 bp |
| RefSeq ORF | 2448 bp |
| Locus ID | 10181 |
| UniProt ID | P52756 |
| Domains | G-patch, RRM, zf-RanBP, zf-C2H2 |
| Protein Families | Druggable Genome |
| Gene Summary | This gene is a candidate tumor suppressor gene which encodes a nuclear RNA binding protein that is a component of the spliceosome A complex. The encoded protein plays a role in the induction of cell cycle arrest and apoptosis through pre-mRNA splicing of multiple target genes including the tumor suppressor protein p53. This gene is located within the tumor suppressor region 3p21.3, and may play a role in the inhibition of tumor transformation and progression of several malignancies including lung cancer. [provided by RefSeq, Oct 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212543 | RBM5 (Myc-DDK-tagged)-Human RNA binding motif protein 5 (RBM5), transcript variant 1 |
CNY 5,968.00 |
|
| RC212543L1 | Lenti ORF clone of Human RNA binding motif protein 5 (RBM5), transcript variant 1, Myc-DDK-tagged |
CNY 8,368.00 |
|
| RC212543L2 | Lenti ORF clone of Human RNA binding motif protein 5 (RBM5), transcript variant 1, mGFP tagged |
CNY 10,550.00 |
|
| RC212543L3 | Lenti ORF clone of Human RNA binding motif protein 5 (RBM5), transcript variant 1, Myc-DDK-tagged |
CNY 10,550.00 |
|
| RC212543L4 | Lenti ORF clone of Human RNA binding motif protein 5 (RBM5), transcript variant 1, mGFP tagged |
CNY 10,550.00 |
|
| SC108553 | RBM5 (untagged)-Human RNA binding motif protein 5 (RBM5), transcript variant 1 |
CNY 5,976.00 |
