HAT1 (NM_001033085) Human Tagged ORF Clone

CAT#: RG209571

  • TrueORF®

HAT1 (tGFP-tagged) - Human histone acetyltransferase 1 (HAT1), transcript variant 2

ORF Plasmid: DDK tGFP



  "NM_001033085" in other vectors (6)

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CNY 4,370.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


KAT1 Rabbit monoclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms histone acetyltransferase 1; KAT1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG209571 representing NM_001033085
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCCGTGTTGAATATGCATCTAAAGTTGATGAGAACTTTGACTGTGTAGAGGCAGATGATGTTGAGG
GCAAAATTAGACAAATCATTCCACCTGGATTTTGCACAAACACGAATGATTTCCTTTCTTTACTGGAAAA
GGAAGTTGATTTCAAGCCATTCGGAACCTTACTTCATACCTACTCAGTTCTCAGTCCAACAGGAGGAGAA
AACTTTACCTTTCAGATATATAAGGCTGACATGACATGTAGAGGCTTTCGAGAATATCATGAAAGGCTTC
AGACCTTTTTGATGTGGTTTATTGAAACTGCTAGCTTTATTGACGTGGATGATGAAAGATGGCACTACTT
TCTAGTATTTGAGAAGTATAATAAGGATGGAGCTACGCTCTTTGCGACCGTAGGCTACATGACAGTCTAT
AATTACTATGTGTACCCAGACAAAACCCGGCCACGTGTAAGTCAGATGCTGATTTTGACTCCATTTCAAG
GTCAAGGCCATGGTGCTCAACTTCTTGAAACAGTTCATAGATACTACACTGAATTTCCTACAGTTCTTGA
TATTACAGCGGAAGATCCATCCAAAAGCTATGTGAAATTACGAGACTTTGTGCTTGTGAAGCTTTGTCAA
GATTTGCCCTGTTTTTCCCGGGAAAAATTAATGCAAGGATTCAATGAAGATATGGCGATAGAGGCACAAC
AGAAGTTCAAAATAAATAAGCAACACGCTAGAAGGGTTTATGAAATTCTTCGACTACTGGTAACTGACAT
GAGTGATGCCGAACAATACAGAAGCTACAGACTGGATATTAAAAGAAGACTAATTAGCCCATATAAGAAA
AAGCAGAGAGATCTTGCTAAGATGAGAAAATGTCTCAGACCAGAAGAACTGACAAACCAGATGAACCAAA
TAGAAATAAGCATGCAACATGAACAGCTGGAAGAGAGTTTTCAGGAACTAGTGGAAGATTACCGGCGTGT
TATTGAACGACTTGCTCAAGAG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG209571 representing NM_001033085
Red=Cloning site Green=Tags(s)

MFRVEYASKVDENFDCVEADDVEGKIRQIIPPGFCTNTNDFLSLLEKEVDFKPFGTLLHTYSVLSPTGGE
NFTFQIYKADMTCRGFREYHERLQTFLMWFIETASFIDVDDERWHYFLVFEKYNKDGATLFATVGYMTVY
NYYVYPDKTRPRVSQMLILTPFQGQGHGAQLLETVHRYYTEFPTVLDITAEDPSKSYVKLRDFVLVKLCQ
DLPCFSREKLMQGFNEDMAIEAQQKFKINKQHARRVYEILRLLVTDMSDAEQYRSYRLDIKRRLISPYKK
KQRDLAKMRKCLRPEELTNQMNQIEISMQHEQLEESFQELVEDYRRVIERLAQE

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001033085
ORF Size 1002 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001033085.1, NP_001028257.1
RefSeq Size 1596 bp
RefSeq ORF 1004 bp
Locus ID 8520
Protein Families Druggable Genome
Gene Summary The protein encoded by this gene is a type B histone acetyltransferase (HAT) that is involved in the rapid acetylation of newly synthesized cytoplasmic histones, which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains. Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. Specifically, this HAT can acetylate soluble but not nucleosomal histone H4 at lysines 5 and 12, and to a lesser degree, histone H2A at lysine 5. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jun 2009]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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