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LPCAT3 (NM_005768) Human Tagged ORF Clone
CAT＃: RG209485
- TrueORF®
LPCAT3 (tGFP-tagged) - Human lysophosphatidylcholine acyltransferase 3 (LPCAT3)
ORF Plasmid: DDK
"NM_005768" in other vectors (6)
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CNY 7,088.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C3F; LPCAT; LPLAT 5; LPSAT; MBOAT5; nessy; OACT5 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG209485 representing NM_005768
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCCTCAGCGGAGGGGGACGAGGGGACTGTGGTGGCGCTGGCGGGGGTTCTGCAGTCGGGTTTCC AGGAGCTGAGCCTTAACAAGTTGGCGACGTCCCTGGGCGCGTCAGAACAGGCGCTGCGGCTGATCATCTC CATCTTCCTGGGTTACCCCTTTGCTTTGTTTTATCGGCATTACCTTTTCTACAAGGAGACCTACCTCATC CACCTCTTCCATACCTTTACAGGCCTCTCAATTGCTTATTTTAACTTTGGAAACCAGCTCTACCACTCCC TGCTGTGTATTGTGCTTCAGTTCCTCATCCTTCGACTAATGGGCCGCACCATCACTGCCGTCCTCACTAC CTTTTGCTTCCAGATGGCCTACCTTCTGGCTGGATACTATTACACTGCCACCGGCAACTACGATATCAAG TGGACAATGCCACATTGTGTTCTGACTTTGAAGCTGATTGGTTTGGCTGTTGACTACTTTGACGGAGGGA AAGATCAGAATTCCTTGTCCTCTGAGCAACAGAAATATGCCATACGTGGTGTTCCTTCCCTGCTGGAAGT TGCTGGTTTCTCCTACTTCTATGGGGCCTTCTTGGTAGGGCCCCAGTTCTCAATGAATCACTACATGAAG CTGGTGCAGGGAGAGCTGATTGACATACCAGGAAAGATACCAAACAGCATCATTCCTGCTCTCAAGCGCC TGAGTCTGGGCCTTTTCTACCTAGTGGGCTACACACTGCTCAGCCCCCACATCACAGAAGACTATCTCCT CACTGAAGACTATGACAACCACCCCTTCTGGTTCCGCTGCATGTACATGCTGATCTGGGGCAAGTTTGTG CTGTACAAATATGTCACCTGTTGGCTGGTCACAGAAGGAGTATGCATTTTGACGGGCCTGGGCTTCAATG GCTTTGAAGAAAAGGGCAAGGCAAAGTGGGATGCCTGTGCCAACATGAAGGTGTGGCTCTTTGAAACAAA CCCCCGCTTCACTGGCACCATTGCCTCATTCAACATCAACACCAACGCCTGGGTGGCCCGCTACATCTTC AAACGACTCAAGTTCCTTGGAAATAAAGAACTCTCTCAGGGTCTCTCGTTGCTATTCCTGGCCCTCTGGC ACGGCCTGCACTCAGGATACCTGGTCTGCTTCCAGATGGAATTCCTCATTGTTATTGTGGAAAGACAGGC TGCCAGGCTCATTCAAGAGAGCCCCACCCTGAGCAAGCTGGCCGCCATTACTGTCCTCCAGCCCTTCTAC TATTTGGTGCAACAGACCATCCACTGGCTCTTCATGGGTTACTCCATGACTGCCTTCTGCCTCTTCACGT GGGACAAATGGCTTAAGGTGTATAAATCCATCTATTTCCTTGGCCACATCTTCTTCCTGAGCCTACTATT CATATTGCCTTATATTCACAAAGCAATGGTGCCAAGGAAAGAGAAGTTAAAGAAGATGGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG209485 representing NM_005768
Red=Cloning site Green=Tags(s) MASSAEGDEGTVVALAGVLQSGFQELSLNKLATSLGASEQALRLIISIFLGYPFALFYRHYLFYKETYLI HLFHTFTGLSIAYFNFGNQLYHSLLCIVLQFLILRLMGRTITAVLTTFCFQMAYLLAGYYYTATGNYDIK WTMPHCVLTLKLIGLAVDYFDGGKDQNSLSSEQQKYAIRGVPSLLEVAGFSYFYGAFLVGPQFSMNHYMK LVQGELIDIPGKIPNSIIPALKRLSLGLFYLVGYTLLSPHITEDYLLTEDYDNHPFWFRCMYMLIWGKFV LYKYVTCWLVTEGVCILTGLGFNGFEEKGKAKWDACANMKVWLFETNPRFTGTIASFNINTNAWVARYIF KRLKFLGNKELSQGLSLLFLALWHGLHSGYLVCFQMEFLIVIVERQAARLIQESPTLSKLAAITVLQPFY YLVQQTIHWLFMGYSMTAFCLFTWDKWLKVYKSIYFLGHIFFLSLLFILPYIHKAMVPRKEKLKKME TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005768 |
| ORF Size | 1461 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005768.6 |
| RefSeq Size | 2297 bp |
| RefSeq ORF | 1464 bp |
| Locus ID | 10162 |
| UniProt ID | Q6P1A2 |
| Domains | MBOAT |
| Protein Families | Transmembrane |
| Gene Summary | Acyltransferase which mediates the conversion of lysophosphatidylcholine (1-acyl-sn-glycero-3-phosphocholine or LPC) into phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine or PC) (LPCAT activity). Catalyzes also the conversion of lysophosphatidylserine (1-acyl-2-hydroxy-sn-glycero-3-phospho-L-serine or LPS) into phosphatidylserine (1,2-diacyl-sn-glycero-3-phospho-L-serine or PS) (LPSAT activity). Has also weak lysophosphatidylethanolamine acyltransferase activity (LPEAT activity). Favors polyunsaturated fatty acyl-CoAs as acyl donors compared to saturated fatty acyl-CoAs. Seems to be the major enzyme contributing to LPCAT activity in the liver. Lysophospholipid acyltransferases (LPLATs) catalyze the reacylation step of the phospholipid remodeling pathway also known as the Lands cycle.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209485 | LPCAT3 (Myc-DDK-tagged)-Human lysophosphatidylcholine acyltransferase 3 (LPCAT3) |
CNY 5,488.00 |
|
| RC209485L1 | Lenti ORF clone of Human lysophosphatidylcholine acyltransferase 3 (LPCAT3), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC209485L2 | Lenti ORF clone of Human lysophosphatidylcholine acyltransferase 3 (LPCAT3), mGFP tagged |
CNY 7,888.00 |
|
| RC209485L3 | Lenti ORF clone of Human lysophosphatidylcholine acyltransferase 3 (LPCAT3), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC209485L4 | Lenti ORF clone of Human lysophosphatidylcholine acyltransferase 3 (LPCAT3), mGFP tagged |
CNY 5,890.00 |
|
| SC116519 | LPCAT3 (untagged)-Human lysophosphatidylcholine acyltransferase 3 (LPCAT3) |
CNY 2,950.00 |
