FNDC4 (NM_022823) Human Tagged ORF Clone

CAT#: RG207471

  • TrueORF®

FNDC4 (tGFP-tagged) - Human fibronectin type III domain containing 4 (FNDC4)

ORF Plasmid: DDK tGFP



  "NM_022823" in other vectors (4)

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CNY 4,000.00

CNY 4,370.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


FNDC4 mouse monoclonal antibody,clone OTI1B7
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms FRCP1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG207471 representing NM_022823
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCAAGCGGATGCCACAGTTCCCCCCCCAGCGGACTCCGTGGGGACATGGCTTCGCTGGTGCCCCTTT
CCCCATATCTAAGCCCCACGGTCCTCCTGCTGGTCAGCTGTGACCTGGGCTTCGTGCGAGCAGACCGGCC
TCCCTCTCCTGTGAATGTGACGGTCACTCACCTCAGAGCCAACTCGGCCACTGTGTCCTGGGACGTCCCA
GAAGGCAACATCGTCATTGGCTACTCCATTTCCCAGCAACGGCAGAATGGCCCCGGGCAGCGTGTGATTC
GGGAGGTGAACACCACCACCCGGGCCTGTGCCCTCTGGGGCCTGGCTGAAGACAGTGACTACACAGTGCA
GGTCAGGAGCATCGGCCTTCGGGGAGAGAGTCCCCCAGGGCCCCGGGTGCACTTCCGAACTCTCAAGGGT
TCTGACCGGCTACCTTCAAACAGTTCAAGCCCAGGTGACATCACAGTGGAAGGTCTGGATGGAGAGCGGC
CACTGCAGACTGGGGAAGTGGTCATCATTGTGGTGGTGTTGCTCATGTGGGCTGCTGTAATTGGGCTGTT
CTGCCGTCAGTATGACATCATCAAGGACAATGACTCCAACAACAATCCCAAGGAGAAGGGAAAGGGGCCG
GAACAGAGTCCTCAGGGAAGGCCAGTGGGGACAAGACAGAAAAAGTCACCATCTATCAACACCATCGACG
TT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG207471 representing NM_022823
Red=Cloning site Green=Tags(s)

MPSGCHSSPPSGLRGDMASLVPLSPYLSPTVLLLVSCDLGFVRADRPPSPVNVTVTHLRANSATVSWDVP
EGNIVIGYSISQQRQNGPGQRVIREVNTTTRACALWGLAEDSDYTVQVRSIGLRGESPPGPRVHFRTLKG
SDRLPSNSSSPGDITVEGLDGERPLQTGEVVIIVVVLLMWAAVIGLFCRQYDIIKDNDSNNNPKEKGKGP
EQSPQGRPVGTRQKKSPSINTIDV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_022823
ORF Size 702 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_022823.3
RefSeq Size 1649 bp
RefSeq ORF 705 bp
Locus ID 64838
UniProt ID Q9H6D8
Domains FN3
Protein Families Transmembrane
Gene Summary Acts as an anti-inflammatory factor in the intestine and colon. Binds to and acts on macrophages to downregulate pro-inflammatory gene expression. Affects key macrophage functions, including phagocytosis, by downregulating many key pathways for macrophage activation, partly via by STAT3 activation and signaling. May be required to dampen the immunological response in colitis.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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