TCF21 (NM_003206) Human Tagged ORF Clone

CAT#: RG206570

  • TrueORF®

TCF21 (tGFP-tagged) - Human transcription factor 21 (TCF21), transcript variant 2

ORF Plasmid: DDK tGFP



  "NM_003206" in other vectors (7)

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CNY 4,000.00

CNY 4,370.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Rabbit Polyclonal Anti-TCF21 Antibody
    • 50 ug

CNY 5,200.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms bHLHa23; POD1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG206570 representing NM_003206
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCACCGGCTCCCTCAGCGATGTGGAGGACCTTCAAGAGGTGGAGATGTTGGAATGTGACGGGTTGA
AAATGGATTCGAACAAGGAATTTGTGACTTCCAACGAGAGCACCGAGGAGAGCTCCAACTGCGAGAATGG
GTCTCCCCAGAAGGGCCGCGGCGGCCTGGGCAAGAGGAGGAAGGCGCCCACCAAGAAGAGCCCCCTGAGC
GGGGTCAGCCAGGAGGGGAAGCAGGTCCAGCGCAACGCCGCCAACGCGCGAGAGCGGGCCCGCATGCGAG
TGCTGAGCAAGGCCTTCTCCAGACTCAAGACCACCCTGCCCTGGGTGCCCCCCGACACCAAGCTCTCCAA
GCTGGACACGCTCAGGCTGGCGTCCAGCTACATCGCCCACTTGAGGCAGATCCTGGCTAACGACAAATAC
GAGAACGGGTACATTCACCCGGTCAACCTGACGTGGCCCTTTATGGTGGCCGGGAAACCCGAGAGTGACC
TGAAAGAAGTGGTGACCGCGAGCCGCTTATGTGGAACCACCGCGTCC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG206570 representing NM_003206
Red=Cloning site Green=Tags(s)

MSTGSLSDVEDLQEVEMLECDGLKMDSNKEFVTSNESTEESSNCENGSPQKGRGGLGKRRKAPTKKSPLS
GVSQEGKQVQRNAANARERARMRVLSKAFSRLKTTLPWVPPDTKLSKLDTLRLASSYIAHLRQILANDKY
ENGYIHPVNLTWPFMVAGKPESDLKEVVTASRLCGTTAS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_003206
ORF Size 537 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003206.4
RefSeq Size 1274 bp
RefSeq ORF 540 bp
Locus ID 6943
UniProt ID O43680
Domains HLH
Protein Families Druggable Genome, Transcription Factors
Gene Summary TCF21 encodes a transcription factor of the basic helix-loop-helix family. The TCF21 product is mesoderm specific, and expressed in embryonic epicardium, mesenchyme-derived tissues of lung, gut, gonad, and both mesenchymal and glomerular epithelial cells in the kidney. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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