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SMAP2 (NM_022733) Human Tagged ORF Clone
CAT＃: RG205875
- TrueORF®
SMAP2 (tGFP-tagged) - Human small ArfGAP2 (SMAP2), transcript variant 1
ORF Plasmid: DDK
"NM_022733" in other vectors (4)
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CNY 4,370.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | SMAP1L |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG205875 representing NM_022733
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAGGCAAGTCGGTGAAGGACGTGGATCGGTACCAGGCTGTCCTGGCCAACCTGCTGCTGGAGGAGG ATAACAAGTTTTGTGCAGATTGCCAGTCTAAAGGGCCGCGATGGGCCTCTTGGAACATTGGTGTGTTCAT CTGCATTCGATGTGCTGGAATCCACAGGAATCTGGGGGTGCACATATCCAGGGTAAAGTCAGTTAACCTC GACCAGTGGACTCAAGAACAGATTCAGTGCATGCAAGAGATGGGAAATGGAAAGGCAAACCGACTTTATG AAGCCTATCTTCCTGAGACCTTTCGGCGACCTCAGATAGACCCAGCTGTTGAAGGATTTATTCGAGACAA ATATGAGAAGAAGAAATACATGGACCGAAGTCTGGACATCAATGCCTTTAGGAAAGAAAAAGATGACAAG TGGAAAAGAGGGAGCGAACCAGTTCCAGAAAAAAAATTGGAACCTGTTGTTTTTGAGAAGGTGAAAATGC CACAGAAAAAAGAAGACCCACAGCTACCTCGGAAAAGCTCCCCGAAATCCACAGCGCCTGTCATGGATTT GTTGGGCCTTGATGCTCCTGTGGCCTGCTCCATTGCAAATAGTAAGACCAGCAATACCCTAGAGAAGGAT TTAGATCTGTTGGCCTCTGTTCCATCCCCTTCTTCTTCCGGTTCCAGAAAGGTTGTAGGTTCCATGCCAA CTGCAGGGAGTGCCGGCTCTGTTCCTGAAAATCTGAACCTGTTTCCGGAGCCAGGGAGCAAATCAGAAGA AATAGGCAAGAAACAGCTCTCTAAAGACTCCATTCTTTCACTGTATGGATCCCAGACGCCTCAAATGCCT ACTCAAGCAATGTTCATGGCTCCCGCTCAGATGGCATATCCCACAGCCTACCCCAGCTTCCCCGGGGTTA CACCTCCTAACAGCATAATGGGGAGCATGATGCCTCCACCAGTAGGCATGGTTGCTCAGCCAGGAGCTTC TGGGATGGTTGCCCCCATGGCCATGCCTGCAGGCTATATGGGTGGCATGCAGGCATCAATGATGGGTGTG CCGAATGGAATGATGACCACCCAGCAGGCTGGCTACATGGCAGGCATGGCAGCTATGCCCCAGACTGTGT ATGGGGTCCAGCCAGCTCAGCAGCTGCAATGGAACCTTACTCAGATGACCCAGCAGATGGCTGGGATGAA CTTCTATGGAGCCAATGGCATGATGAACTATGGACAGTCAATGAGTGGCGGAAATGGACAGGCAGCAAAT CAGACTCTCAGTCCTCAGATGTGGAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG205875 representing NM_022733
Red=Cloning site Green=Tags(s) MTGKSVKDVDRYQAVLANLLLEEDNKFCADCQSKGPRWASWNIGVFICIRCAGIHRNLGVHISRVKSVNL DQWTQEQIQCMQEMGNGKANRLYEAYLPETFRRPQIDPAVEGFIRDKYEKKKYMDRSLDINAFRKEKDDK WKRGSEPVPEKKLEPVVFEKVKMPQKKEDPQLPRKSSPKSTAPVMDLLGLDAPVACSIANSKTSNTLEKD LDLLASVPSPSSSGSRKVVGSMPTAGSAGSVPENLNLFPEPGSKSEEIGKKQLSKDSILSLYGSQTPQMP TQAMFMAPAQMAYPTAYPSFPGVTPPNSIMGSMMPPPVGMVAQPGASGMVAPMAMPAGYMGGMQASMMGV PNGMMTTQQAGYMAGMAAMPQTVYGVQPAQQLQWNLTQMTQQMAGMNFYGANGMMNYGQSMSGGNGQAAN QTLSPQMWK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_022733 |
| ORF Size | 1287 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_022733.3 |
| RefSeq Size | 2618 bp |
| RefSeq ORF | 1290 bp |
| Locus ID | 64744 |
| UniProt ID | Q8WU79 |
| Domains | ArfGap |
| Protein Pathways | Endocytosis |
| Gene Summary | GTPase activating protein that acts on ARF1. Can also activate ARF6 (in vitro). May play a role in clathrin-dependent retrograde transport from early endosomes to the trans-Golgi network (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205875 | SMAP2 (Myc-DDK-tagged)-Human small ArfGAP2 (SMAP2), transcript variant 1 |
CNY 3,656.00 |
|
| RC205875L3 | Lenti ORF clone of Human small ArfGAP2 (SMAP2), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205875L4 | Lenti ORF clone of Human small ArfGAP2 (SMAP2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| SC100376 | SMAP2 (untagged)-Human small ArfGAP2 (SMAP2), transcript variant 1 |
CNY 7,220.00 |
