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GERP (TRIM8) (NM_030912) Human Tagged ORF Clone
CAT＃: RG205812
- TrueORF®
TRIM8 (tGFP-tagged) - Human tripartite motif containing 8 (TRIM8)
ORF Plasmid: DDK
"NM_030912" in other vectors (6)
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CNY 7,752.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | GERP; RNF27 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG205812 representing NM_030912
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGAATTGGAAGAACTGCTTCGAGGAGGAGCTCATCTGCCCTATCTGCCTGCACGTTTTCGTGG AGCCAGTGCAGCTGCCGTGCAAACACAACTTCTGCCGGGGCTGCATCGGCGAGGCGTGGGCCAAGGACAG CGGCCTCGTACGCTGCCCAGAGTGCAACCAGGCCTACAACCAGAAGCCGGGCCTGGAGAAGAACCTGAAG CTCACCAACATCGTGGAGAAGTTCAATGCCCTGCACGTGGAGAAGCCGCCGGCGGCGCTGCACTGCGTGT TCTGCCGCCGCGGCCCCCCGCTGCCCGCGCAGAAGGTCTGCCTGCGCTGCGAGGCGCCCTGCTGCCAGTC CCACGTGCAGACGCACCTGCAGCAGCCCTCCACCGCCCGCGGGCACCTCCTGGTGGAGGCGGACGACGTG CGGGCCTGGAGCTGCCCGCAGCACAACGCCTACCGCCTCTACCACTGCGAGGCCGAGCAGGTGGCCGTGT GCCAGTACTGCTGCTACTACAGCGGCGCGCATCAGGGACACTCGGTGTGCGACGTGGAGATCCGAAGGAA TGAAATCCGGAAGATGCTCATGAAGCAGCAGGACCGGCTGGAGGAGCGAGAGCAGGACATTGAGGACCAG CTGTACAAACTCGAGTCAGACAAGCGCCTGGTGGAGGAGAAAGTGAACCAACTGAAGGAGGAAGTTCGGC TGCAGTACGAGAAGCTGCACCAGCTGCTGGACGAGGACCTGCGGCAGACAGTGGAGGTCCTAGACAAGGC CCAGGCCAAGTTCTGCAGCGAGAACGCAGCGCAGGCGCTGCACCTCGGGGAGCGCATGCAGGAGGCCAAG AAGCTGCTGGGCTCCCTGCAGCTGCTCTTTGATAAGACGGAGGATGTCAGCTTCATGAAGAACACCAAGT CTGTGAAAATCCTGATGGACAGGACCCAGACCTGCACGAGCAGCAGCCTTTCCCCCACTAAGATCGGCCA CCTGAACTCCAAGCTCTTCCTGAACGAAGTGGCCAAGAAGGAGAAGCAGCTGCGGAAAATGCTAGAAGGC CCCTTCAGCACGCCGGTGCCCTTCCTGCAGAGTGTCCCCCTGTACCCTTGCGGCGTGAGCAGCTCTGGGG CGGAAAAGCGCAAGCACTCAACGGCCTTCCCAGAGGCCAGTTTCCTAGAGACGTCGTCGGGCCCTGTGGG CGGCCAGTACGGGGCGGCGGGCACAGCCAGCGGTGAGGGCCAGTCTGGGCAGCCCCTGGGGCCCTGCAGC TCCACGCAGCACTTGGTGGCCCTGCCGGGCGGCGCCCAACCAGTGCACTCAAGCCCCGTGTTCCCCCCAT CGCAGTATCCCAATGGCTCCGCCGCCCAGCAGCCCATGCTCCCCCAGTATGGCGGCCGCAAGATTCTCGT CTGTTCTGTGGACAACTGTTACTGTTCTTCCGTGGCCAACCATGGCGGCCACCAGCCCTACCCCCGCTCC GGCCACTTTCCCTGGACAGTGCCCTCGCAGGAGTACTCACACCCGCTCCCGCCCACACCCTCCGTCCCCC AGTCCCTTCCCAGCCTGGCGGTCAGAGACTGGCTTGACGCCTCCCAGCAGCCCGGCCACCAGGATTTCTA CAGGGTGTATGGGCAGCCGTCCACCAAACACTACGTGACGAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG205812 representing NM_030912
Red=Cloning site Green=Tags(s) MAENWKNCFEEELICPICLHVFVEPVQLPCKHNFCRGCIGEAWAKDSGLVRCPECNQAYNQKPGLEKNLK LTNIVEKFNALHVEKPPAALHCVFCRRGPPLPAQKVCLRCEAPCCQSHVQTHLQQPSTARGHLLVEADDV RAWSCPQHNAYRLYHCEAEQVAVCQYCCYYSGAHQGHSVCDVEIRRNEIRKMLMKQQDRLEEREQDIEDQ LYKLESDKRLVEEKVNQLKEEVRLQYEKLHQLLDEDLRQTVEVLDKAQAKFCSENAAQALHLGERMQEAK KLLGSLQLLFDKTEDVSFMKNTKSVKILMDRTQTCTSSSLSPTKIGHLNSKLFLNEVAKKEKQLRKMLEG PFSTPVPFLQSVPLYPCGVSSSGAEKRKHSTAFPEASFLETSSGPVGGQYGAAGTASGEGQSGQPLGPCS STQHLVALPGGAQPVHSSPVFPPSQYPNGSAAQQPMLPQYGGRKILVCSVDNCYCSSVANHGGHQPYPRS GHFPWTVPSQEYSHPLPPTPSVPQSLPSLAVRDWLDASQQPGHQDFYRVYGQPSTKHYVTS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_030912 |
| ORF Size | 1653 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_030912.3 |
| RefSeq Size | 2645 bp |
| RefSeq ORF | 1656 bp |
| Locus ID | 81603 |
| UniProt ID | Q9BZR9 |
| Domains | RING |
| Gene Summary | This gene encodes a member of the tripartite motif (TRIM) protein family. Based on similarities to other proteins, the encoded protein is suspected to be an E3 ubiquitin-protein ligase. Regulation of this gene may be altered in some cancers. Mutations resulting in a truncated protein product have been observed in early-onset epileptic encephalopathy (EOEE). [provided by RefSeq, Sep 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205812 | TRIM8 (Myc-DDK-tagged)-Human tripartite motif containing 8 (TRIM8) |
CNY 6,152.00 |
|
| RC205812L1 | Lenti ORF clone of Human tripartite motif containing 8 (TRIM8), Myc-DDK-tagged |
CNY 8,552.00 |
|
| RC205812L2 | Lenti ORF clone of Human tripartite motif containing 8 (TRIM8), mGFP tagged |
CNY 8,552.00 |
|
| RC205812L3 | Lenti ORF clone of Human tripartite motif containing 8 (TRIM8), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205812L4 | Lenti ORF clone of Human tripartite motif containing 8 (TRIM8), mGFP tagged |
CNY 5,890.00 |
|
| SC109091 | TRIM8 (untagged)-Human tripartite motif containing 8 (TRIM8) |
CNY 6,168.00 |
