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Semaphorin 3c (SEMA3C) (NM_006379) Human Tagged ORF Clone
CAT＃: RG205269
- TrueORF®
SEMA3C (tGFP-tagged) - Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C (SEMA3C)
ORF Plasmid: DDK
"NM_006379" in other vectors (6)
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CNY 6,370.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | SEMAE; SemE |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG205269 representing NM_006379
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCATTCCGGACAATTTGCGTGTTGGTTGGAGTATTTATTTGTTCTATCTGTGTGAAAGGATCTTCCC AGCCCCAAGCAAGAGTTTATTTAACATTTGATGAACTTCGAGAAACCAAGACCTCTGAATACTTCAGCCT TTCCCACCATCCTTTAGACTACAGGATTTTATTAATGGATGAAGATCAGGACCGGATATATGTGGGAAGC AAAGATCACATTCTTTCCCTGAATATTAACAATATAAGTCAAGAAGCTTTGAGTGTTTTCTGGCCAGCAT CTACAATCAAAGTTGAAGAATGCAAAATGGCTGGCAAAGATCCCACACACGGCTGTGGGAACTTTGTCCG TGTAATTCAGACTTTCAATCGCACACATTTGTATGTCTGTGGGAGTGGCGCTTTCAGTCCTGTCTGTACT TACTTGAACAGAGGGAGGAGATCAGAGGACCAAGTTTTCATGATTGACTCCAAGTGTGAATCTGGAAAAG GACGCTGCTCTTTCAACCCCAACGTGAACACGGTGTCTGTTATGATCAATGAGGAGCTTTTCTCTGGAAT GTATATAGATTTCATGGGGACAGATGCTGCTATTTTTCGAAGTTTAACCAAGAGGAATGCGGTCAGAACT GATCAACATAATTCCAAATGGCTAAGTGAACCTATGTTTGTAGATGCACATGTCATCCCAGATGGTACTG ATCCAAATGATGCTAAGGTGTACTTCTTCTTCAAAGAAAAACTGACTGACAATAACAGGAGCACGAAACA GATTCATTCCATGATTGCTCGAATATGTCCTAATGACACTGGTGGACTGCGTAGCCTTGTCAACAAGTGG ACCACTTTCTTAAAGGCGAGGCTGGTGTGCTCGGTAACAGATGAAGACGGCCCAGAAACACACTTTGATG AATTAGAGGATGTGTTTCTGCTGGAAACTGATAACCCGAGGACAACACTAGTGTATGGCATTTTTACAAC ATCAAGCTCAGTTTTCAAAGGATCAGCCGTGTGTGTGTATCATTTATCTGATATACAGACTGTGTTTAAT GGGCCTTTTGCCCACAAAGAAGGGCCCAATCATCAGCTGATTTCCTATCAGGGCAGAATTCCATATCCTC GCCCTGGAACTTGTCCAGGAGGAGCATTTACACCCAATATGCGAACCACCAAGGAGTTCCCAGATGATGT TGTCACTTTTATTCGGAACCATCCTCTCATGTACAATTCCATCTACCCAATCCACAAAAGGCCTTTGATT GTTCGTATTGGCACTGACTACAAGTATACAAAGATAGCTGTGGATCGAGTGAACGCTGCTGATGGGAGAT ACCATGTCCTGTTTCTCGGAACAGATCGGGGTACTGTGCAAAAAGTGGTTGTTCTTCCTACTAACAACTC TGTCAGTGGCGAGCTCATTCTGGAGGAGCTGGAAGTCTTTAAGAATCATGCTCCTATAACAACAATGAAA ATTTCATCTAAAAAGCAACAGTTGTATGTGAGTTCCAATGAAGGGGTTTCCCAGGTATCTCTGCACCGCT GCCACATCTATGGTACAGCCTGTGCTGACTGCTGCCTGGCGCGGGACCCTTATTGCGCCTGGGATGGCCA TTCCTGTTCCAGATTCTACCCAACTGGGAAACGGAGGAGCCGAAGACAAGATGTGAGACATGGAAACCCA CTGACTCAATGCAGAGGATTTAATCTAAAAGCATACAGAAATGCAGCTGAAATTGTGCAGTATGGAGTAA AAAATAACACCACTTTTCTGGAGTGTGCCCCCAAGTCTCCGCAGGCATCTATCAAGTGGCTGTTACAGAA AGACAAAGACAGGAGGAAAGAGGTTAAGCTGAATGAACGAATAATAGCCACTTCACAGGGACTCCTGATC CGCTCTGTTCAGGGTTCTGACCAAGGACTTTATCACTGCATTGCTACAGAAAATAGTTTCAAGCAGACCA TAGCCAAGATCAACTTCAAAGTTTTAGATTCAGAAATGGTGGCTGTTGTGACGGACAAATGGTCCCCGTG GACCTGGGCCAGCTCTGTGAGGGCTTTACCCTTCCACCCGAAGGACATCATGGGGGCATTCAGCCACTCA GAAATGCAGATGATTAACCAATACTGCAAAGACACTCGGCAGCAACATCAGCAGGGAGATGAATCACAGA AAATGAGAGGGGACTATGGCAAGTTAAAGGCCCTCATCAATAGTCGGAAAAGTAGAAACAGGAGGAATCA GTTGCCAGAGTCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG205269 representing NM_006379
Red=Cloning site Green=Tags(s) MAFRTICVLVGVFICSICVKGSSQPQARVYLTFDELRETKTSEYFSLSHHPLDYRILLMDEDQDRIYVGS KDHILSLNINNISQEALSVFWPASTIKVEECKMAGKDPTHGCGNFVRVIQTFNRTHLYVCGSGAFSPVCT YLNRGRRSEDQVFMIDSKCESGKGRCSFNPNVNTVSVMINEELFSGMYIDFMGTDAAIFRSLTKRNAVRT DQHNSKWLSEPMFVDAHVIPDGTDPNDAKVYFFFKEKLTDNNRSTKQIHSMIARICPNDTGGLRSLVNKW TTFLKARLVCSVTDEDGPETHFDELEDVFLLETDNPRTTLVYGIFTTSSSVFKGSAVCVYHLSDIQTVFN GPFAHKEGPNHQLISYQGRIPYPRPGTCPGGAFTPNMRTTKEFPDDVVTFIRNHPLMYNSIYPIHKRPLI VRIGTDYKYTKIAVDRVNAADGRYHVLFLGTDRGTVQKVVVLPTNNSVSGELILEELEVFKNHAPITTMK ISSKKQQLYVSSNEGVSQVSLHRCHIYGTACADCCLARDPYCAWDGHSCSRFYPTGKRRSRRQDVRHGNP LTQCRGFNLKAYRNAAEIVQYGVKNNTTFLECAPKSPQASIKWLLQKDKDRRKEVKLNERIIATSQGLLI RSVQGSDQGLYHCIATENSFKQTIAKINFKVLDSEMVAVVTDKWSPWTWASSVRALPFHPKDIMGAFSHS EMQMINQYCKDTRQQHQQGDESQKMRGDYGKLKALINSRKSRNRRNQLPES TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006379 |
| ORF Size | 2253 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006379.2, NP_006370.1 |
| RefSeq Size | 5189 bp |
| RefSeq ORF | 2256 bp |
| Locus ID | 10512 |
| UniProt ID | Q99985 |
| Domains | Sema, IG, PSI |
| Protein Families | Secreted Protein |
| Protein Pathways | Axon guidance |
| Gene Summary | This gene encodes a secreted glycoprotein that belongs to the semaphorin class 3 family of neuronal guidance cues. The encoded protein contains an N-terminal sema domain, integrin and immunoglobulin-like domains, and a C-terminal basic domain. Homodimerization and proteolytic cleavage of the C-terminal propeptide are necessary for the function of the encoded protein. It binds a neuropilin co-receptor before forming a heterotrimeric complex with an associated plexin. An increase in the expression of this gene correlates with an increase in cancer cell invasion and adhesion. Naturally occurring mutations in this gene are associated with Hirschsprung disease. [provided by RefSeq, May 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205269 | SEMA3C (Myc-DDK-tagged)-Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C (SEMA3C) |
CNY 5,504.00 |
|
| RC205269L1 | Lenti ORF clone of Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C (SEMA3C), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC205269L2 | Lenti ORF clone of Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C (SEMA3C), mGFP tagged |
CNY 7,700.00 |
|
| RC205269L3 | Lenti ORF clone of Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C (SEMA3C), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC205269L4 | Lenti ORF clone of Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C (SEMA3C), mGFP tagged |
CNY 7,700.00 |
|
| SC116160 | SEMA3C (untagged)-Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C (SEMA3C) |
CNY 2,950.00 |
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