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CPSF1 (NM_013291) Human Tagged ORF Clone
CAT＃: RG203519
- TrueORF®
CPSF1 (tGFP-tagged) - Human cleavage and polyadenylation specific factor 1, 160kDa (CPSF1)
ORF Plasmid: DDK
"NM_013291" in other vectors (6)
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CNY 13,590.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CPSF160; HSU37012; MYP27; P/cl.18 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG203519 representing NM_013291
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACGCCGTGTACAAACAGGCGCATCCGCCCACCGGTCTGGAGTTCTCCATGTACTGCAACTTCTTCA ACAACAGCGAGCGCAACCTGGTAGTGGCCGGGACCTCGCAGCTCTACGTGTACCGCCTCAACCGCGACGC CGAGGCTCTGACCAAGAATGACAGGAGCACAGAGGGGAAGGCCCACCGGGAGAAGCTCGAGCTTGCTGCC TCCTTCTCCTTCTTTGGCAACGTCATGTCCATGGCCAGCGTGCAGCTGGCAGGAGCCAAGCGGGATGCCC TGCTCCTAAGCTTCAAGGATGCCAAGCTGTCTGTGGTGGAGTACGACCCGGGCACCCATGACCTGAAGAC CCTGTCACTGCACTACTTTGAGGAGCCTGAGCTTCGGGACGGGTTTGTGCAGAATGTACACACGCCGCGA GTGCGGGTGGACCCCGACGGGCGCTGTGCAGCCATGCTTGTCTACGGCACGCGGCTGGTGGTCCTGCCCT TCCGCAGGGAGAGCCTGGCTGAGGAGCACGAGGGGCTCGTGGGTGAGGGGCAGAGGTCCAGCTTCCTGCC CAGCTACATCATCGACGTGCGGGCCCTAGACGAGAAGCTGCTCAACATCATCGACCTGCAGTTCCTGCAT GGCTACTACGAGCCTACCCTCCTCATCCTGTTTGAGCCCAACCAGACCTGGCCTGGGCGCGTGGCCGTGC GGCAGGACACGTGCTCCATTGTGGCCATCTCACTGAACATCACGCAGAAGGTGCACCCCGTCATCTGGTC CCTCACCAGCCTGCCCTTTGACTGCACCCAGGCTCTGGCTGTGCCCAAGCCCATAGGTGGGGTGGTGGTG TTTGCCGTCAACTCGCTGTTGTACCTGAACCAGAGCGTCCCCCCGTATGGCGTGGCTCTCAACAGCCTCA CCACAGGAACCACGGCTTTCCCGCTTCGCACCCAGGAGGGTGTGCGGATCACCCTGGACTGCGCCCAGGC CACCTTCATCTCCTACGACAAGATGGTCATCTCCCTCAAGGGCGGCGAGATCTACGTGCTGACCCTCATC ACCGACGGCATGCGCAGTGTCCGAGCGTTCCACTTTGACAAGGCGGCCGCCAGCGTCCTCACCACCAGCA TGGTCACCATGGAGCCCGGGTACCTGTTCCTGGGTTCTCGCCTGGGCAATTCCCTCCTCCTCAAGTACAC GGAGAAGCTGCAGGAGCCCCCGGCCAGTGCTGTCCGTGAGGCTGCCGACAAGGAAGAGCCTCCCTCAAAG AAGAAGCGAGTGGATGCGACGGCCGGCTGGTCAGCTGCGGGTAAGTCGGTGCCGCAGGATGAGGTGGACG AGATTGAAGTGTACGGCAGCGAGGCCCAGTCGGGAACACAGCTGGCCACCTACTCCTTTGAGGTGTGTGA CAGCATCCTGAACATTGGACCCTGTGCCAATGCCGCCGTGGGCGAGCCTGCCTTCCTCTCTGAAGAGTTT CAGAACAGCCCCGAGCCGGACCTGGAGATTGTGGTTTGCTCCGGCCACGGGAAGAACGGGGCTTTGTCGG TGCTGCAGAAGAGCATCCGGCCCCAGGTGGTGACAACCTTTGAGCTTCCCGGCTGCTATGACATGTGGAC AGTCATCGCCCCGGTGCGTAAGGAGGAGGAGGACAATCCCAAGGGGGAGGGCACAGAGCAGGAACCCAGC ACCACCCCTGAAGCAGACGACGACGGCCGCAGACACGGATTCCTGATTCTGAGCCGGGAAGACTCCACCA TGATCCTGCAGACGGGGCAGGAGATCATGGAGCTGGACACCAGTGGCTTCGCCACTCAGGGCCCCACGGT CTTTGCTGGGAACATCGGGGACAACCGCTACATTGTCCAAGTGTCACCACTGGGCATCCGCCTGCTGGAA GGAGTGAATCAGCTGCACTTCATCCCCGTGGACCTGGGCGCCCCCATCGTGCAGTGCGCCGTGGCCGACC CCTATGTGGTCATCATGAGTGCCGAGGGCCACGTCACCATGTTCCTGCTGAAGAGTGACTCCTACGGTGG CCGCCACCACCGCCTGGCGCTGCACAAGCCCCCGCTGCACCATCAGTCCAAGGTGATTACGCTGTGCCTG TACCGAGACCTCAGCGGCATGTTCACCACTGAGAGCCGCCTGGGTGGGGCCCGTGACGAGCTCGGGGGCC GCAGTGGCCCGGAGGCCGAGGGCCTGGGCTCAGAGACTAGCCCCACAGTGGATGACGAGGAGGAGATGCT GTATGGGGATTCGGGCTCCCTCTTCAGCCCCAGCAAGGAGGAGGCCCGAAGAAGCAGCCAGCCCCCTGCT GACCGGGACCCTGCACCCTTCCGGGCAGAGCCTACCCACTGGTGCCTGCTGGTGCGGGAGAATGGCACCA TGGAGATCTACCAGCTTCCCGACTGGCGGCTGGTGTTCCTGGTGAAGAACTTCCCTGTGGGGCAGCGGGT CCTTGTGGACAGCTCCTTTGGACAGCCCACTACACAGGGCGAGGCCCGCAGGGAGGAGGCCACGCGCCAG GGGGAGCTGCCCCTCGTCAAGGAGGTGCTGCTGGTGGCGCTGGGCAGCCGCCAGAGCAGGCCCTACCTGC TGGTGCATGTGGACCAAGAGCTGCTTATCTACGAGGCCTTCCCCCACGACTCTCAGCTCGGCCAGGGCAA TCTCAAAGTCCGCTTTAAGAAGGTCCCTCACAACATCAACTTCCGTGAGAAGAAGCCAAAGCCATCCAAG AAGAAAGCAGAAGGTGGCGGCGCAGAGGAGGGGGCTGGGGCCCGGGGCCGCGTGGCGCGTTTCCGCTACT TCGAGGATATTTATGGCTACTCAGGGGTCTTCATCTGCGGCCCCTCCCCTCACTGGCTCTTGGTGACCGG CCGAGGGGCTCTGCGGCTACACCCCATGGCCATCGACGGCCCGGTCGACTCTTTCGCTCCATTCCACAAT GTCAACTGTCCCCGCGGCTTCCTGTACTTCAACAGACAGGGCGAGCTGAGGATCAGTGTCCTGCCTGCCT ACCTGTCCTATGATGCCCCATGGCCTGTCAGGAAGATCCCGCTGCGCTGCACGGCCCACTATGTGGCTTA CCACGTGGAGTCTAAGGTGTATGCTGTGGCCACCAGCACCAACACGCCGTGTGCCCGCATCCCACGCATG ACTGGCGAGGAGAAGGAGTTTGAGACCATCGAGAGAGATGAGCGGTACATCCACCCCCAGCAGGAGGCCT TCTCCATCCAGCTCATCTCCCCGGTCAGCTGGGAGGCTATTCCCAATGCCAGGATCGAGCTGCAGGAGTG GGAGCATGTGACCTGCATGAAGACAGTGTCTCTGCGCAGTGAGGAGACCGTGTCGGGCCTCAAAGGCTAC GTGGCCGCCGGGACCTGCCTCATGCAGGGGGAGGAGGTCACGTGCCGAGGGCGGATCTTGATCATGGATG TGATTGAGGTGGTGCCCGAGCCTGGCCAGCCCTTGACCAAGAACAAGTTCAAAGTCCTTTACGAGAAGGA GCAGAAGGGGCCCGTGACCGCCCTGTGCCACTGCAATGGCCACCTGGTGTCGGCCATCGGCCAGAAGATT TTCCTGTGGAGCCTGCGGGCCAGCGAGCTGACGGGCATGGCCTTCATCGACACGCAGCTCTACATACACC AGATGATCAGCGTCAAGAACTTCATCCTGGCAGCCGACGTCATGAAGAGCATTTCGCTGCTGCGCTACCA GGAGGAAAGCAAGACGCTGAGCCTGGTGTCGCGGGATGCCAAGCCCCTGGAGGTGTACAGCGTGGACTTC ATGGTGGACAATGCCCAGCTGGGTTTTCTGGTGTCTGACCGCGACCGCAACCTCATGGTGTACATGTACC TGCCCGAAGCCAAGGAGAGTTTCGGGGGCATGCGCCTGCTGCGTCGGGCAGACTTCCACGTGGGTGCCCA CGTGAACACGTTCTGGAGGACCCCGTGCCGGGGGGCCACTGAAGGGCTCAGCAAAAAGTCGGTCGTGTGG GAGAATAAGCACATCACGTGGTTTGCCACCCTGGACGGCGGCATCGGGCTGCTGCTGCCCATGCAGGAGA AGACCTACCGGCGGCTGCTGATGCTGCAGAACGCGCTGACCACCATGCTGCCACACCACGCCGGCCTCAA CCCCCGCGCCTTCCGGATGCTGCACGTGGACCGCCGCACCCTCCAGAATGCCGTGCGCAACGTGCTGGAT GGGGAGCTGCTCAACCGCTACCTGTACCTGAGCACCATGGAGCGCAGCGAGCTAGCCAAGAAGATCGGCA CCACACCAGACATAATCCTGGACGACTTGCTGGAGACGGACCGCGTCACCGCCCACTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG203519 representing NM_013291
Red=Cloning site Green=Tags(s) MYAVYKQAHPPTGLEFSMYCNFFNNSERNLVVAGTSQLYVYRLNRDAEALTKNDRSTEGKAHREKLELAA SFSFFGNVMSMASVQLAGAKRDALLLSFKDAKLSVVEYDPGTHDLKTLSLHYFEEPELRDGFVQNVHTPR VRVDPDGRCAAMLVYGTRLVVLPFRRESLAEEHEGLVGEGQRSSFLPSYIIDVRALDEKLLNIIDLQFLH GYYEPTLLILFEPNQTWPGRVAVRQDTCSIVAISLNITQKVHPVIWSLTSLPFDCTQALAVPKPIGGVVV FAVNSLLYLNQSVPPYGVALNSLTTGTTAFPLRTQEGVRITLDCAQATFISYDKMVISLKGGEIYVLTLI TDGMRSVRAFHFDKAAASVLTTSMVTMEPGYLFLGSRLGNSLLLKYTEKLQEPPASAVREAADKEEPPSK KKRVDATAGWSAAGKSVPQDEVDEIEVYGSEAQSGTQLATYSFEVCDSILNIGPCANAAVGEPAFLSEEF QNSPEPDLEIVVCSGHGKNGALSVLQKSIRPQVVTTFELPGCYDMWTVIAPVRKEEEDNPKGEGTEQEPS TTPEADDDGRRHGFLILSREDSTMILQTGQEIMELDTSGFATQGPTVFAGNIGDNRYIVQVSPLGIRLLE GVNQLHFIPVDLGAPIVQCAVADPYVVIMSAEGHVTMFLLKSDSYGGRHHRLALHKPPLHHQSKVITLCL YRDLSGMFTTESRLGGARDELGGRSGPEAEGLGSETSPTVDDEEEMLYGDSGSLFSPSKEEARRSSQPPA DRDPAPFRAEPTHWCLLVRENGTMEIYQLPDWRLVFLVKNFPVGQRVLVDSSFGQPTTQGEARREEATRQ GELPLVKEVLLVALGSRQSRPYLLVHVDQELLIYEAFPHDSQLGQGNLKVRFKKVPHNINFREKKPKPSK KKAEGGGAEEGAGARGRVARFRYFEDIYGYSGVFICGPSPHWLLVTGRGALRLHPMAIDGPVDSFAPFHN VNCPRGFLYFNRQGELRISVLPAYLSYDAPWPVRKIPLRCTAHYVAYHVESKVYAVATSTNTPCARIPRM TGEEKEFETIERDERYIHPQQEAFSIQLISPVSWEAIPNARIELQEWEHVTCMKTVSLRSEETVSGLKGY VAAGTCLMQGEEVTCRGRILIMDVIEVVPEPGQPLTKNKFKVLYEKEQKGPVTALCHCNGHLVSAIGQKI FLWSLRASELTGMAFIDTQLYIHQMISVKNFILAADVMKSISLLRYQEESKTLSLVSRDAKPLEVYSVDF MVDNAQLGFLVSDRDRNLMVYMYLPEAKESFGGMRLLRRADFHVGAHVNTFWRTPCRGATEGLSKKSVVW ENKHITWFATLDGGIGLLLPMQEKTYRRLLMLQNALTTMLPHHAGLNPRAFRMLHVDRRTLQNAVRNVLD GELLNRYLYLSTMERSELAKKIGTTPDIILDDLLETDRVTAHF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013291 |
| ORF Size | 4329 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013291.3 |
| RefSeq Size | 4494 bp |
| RefSeq ORF | 4332 bp |
| Locus ID | 29894 |
| UniProt ID | Q10570 |
| Domains | CPSF_A |
| Gene Summary | Cleavage and polyadenylation specificity factor (CPSF) is a multisubunit complex that plays a central role in 3-prime processing of pre-mRNAs. CPSF recognizes the AAUAAA signal in the pre-mRNA and interacts with other proteins to facilitate both RNA cleavage and poly(A) synthesis. CPSF1 is the largest subunit of the CPSF complex (Murthy and Manley, 1995 [PubMed 7590244]).[supplied by OMIM, Mar 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203519 | CPSF1 (Myc-DDK-tagged)-Human cleavage and polyadenylation specific factor 1, 160kDa (CPSF1) |
CNY 7,600.00 |
|
| RC203519L1 | Lenti-ORF clone of CPSF1 (Myc-DDK-tagged)-Human cleavage and polyadenylation specific factor 1, 160kDa (CPSF1) |
CNY 12,272.00 |
|
| RC203519L2 | Lenti-ORF clone of CPSF1 (mGFP-tagged)-Human cleavage and polyadenylation specific factor 1, 160kDa (CPSF1) |
CNY 12,272.00 |
|
| RC203519L3 | Lenti-ORF clone of CPSF1 (Myc-DDK-tagged)-Human cleavage and polyadenylation specific factor 1, 160kDa (CPSF1) |
CNY 14,250.00 |
|
| RC203519L4 | Lenti-ORF clone of CPSF1 (mGFP-tagged)-Human cleavage and polyadenylation specific factor 1, 160kDa (CPSF1) |
CNY 12,272.00 |
|
| SC115303 | CPSF1 (untagged)-Human cleavage and polyadenylation specific factor 1, 160kDa (CPSF1) |
CNY 9,880.00 |
