DPAGT1 (NM_001382) Human Tagged ORF Clone
CAT#: RG201787
- TrueORF®
DPAGT1 (tGFP-tagged) - Human dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) (DPAGT1)
ORF Plasmid: DDK
"NM_001382" in other vectors (6)
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CNY 5,256.00
Cited in 1 publication. |
CNY 1,000.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | ALG7; CDG-Ij; CDG1J; CMS13; CMSTA2; D11S366; DGPT; DPAGT; DPAGT2; G1PT; GPT; UAGT; UGAT |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG201787 representing NM_001382
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGGCCTTCTCGGAATTGCCCATGCCGCTGCTGATCAATTTGATCGTCTCGCTGCTGGGATTTGTGG CCACAGTCACCCTCATCCCGGCCTTCCGGGGCCACTTCATTGCTGCGCGCCTCTGTGGTCAGGACCTCAA CAAAACCAGCCGACAGCAGATCCCAGAATCCCAGGGAGTGATCAGCGGTGCTGTTTTCCTTATCATCCTC TTCTGCTTCATCCCTTTCCCCTTCCTGAACTGCTTTGTGAAGGAGCAGTGTAAGGCATTCCCCCACCATG AATTTGTGGCCCTGATAGGTGCCCTCCTTGCCATCTGCTGCATGATCTTCCTGGGCTTTGCGGATGATGT ACTGAATCTGCGCTGGCGCCATAAGCTGCTGCTACCTACAGCTGCCTCACTACCTCTCCTCATGGTCTAT TTCACCAACTTTGGCAACACGACCATTGTGGTGCCCAAGCCCTTCCGCCCGATACTTGGCCTGCATCTGG ACTTGGGAATCCTGTACTATGTCTACATGGGGCTGCTGGCAGTGTTCTGTACCAATGCCATCAATATCCT AGCAGGAATTAACGGCCTAGAGGCTGGCCAGTCACTAGTCATTTCTGCTTCCATCATTGTCTTCAACCTG GTAGAGTTGGAAGGTGATTGTCGGGATGATCATGTCTTTTCCCTCTACTTCATGATACCCTTTTTTTTCA CCACTTTGGGATTGCTCTACCACAACTGGTACCCATCACGGGTGTTTGTGGGAGATACCTTCTGTTACTT TGCTGGCATGACCTTTGCCGTGGTGGGCATCTTGGGACACTTCAGCAAGACCATGCTACTATTCTTCATG CCCCAGGTGTTCAACTTCCTCTACTCACTGCCTCAGCTCCTGCATATCATCCCCTGCCCTCGCCACCGCA TACCCAGACTCAATATCAAGACAGGCAAACTGGAGATGAGCTATTCCAAGTTCAAGACCAAGAGCCTCTC TTTCTTGGGCACCTTTATTTTAAAGGTGGCAGAGAGCCTCCAGCTGGTGACAGTACACCAGAGTGAGACT GAAGATGGTGAATTCACTGAATGTAACAACATGACCCTCATCAACTTGCTACTTAAAGTCCTTGGGCCCA TACATGAGAGAAACCTCACATTGCTCCTGCTGCTGCTGCAGATCCTGGGCAGTGCCATCACCTTCTCCAT TCGATATCAGCTCGTTCGACTCTTCTATGATGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG201787 representing NM_001382
Red=Cloning site Green=Tags(s) MWAFSELPMPLLINLIVSLLGFVATVTLIPAFRGHFIAARLCGQDLNKTSRQQIPESQGVISGAVFLIIL FCFIPFPFLNCFVKEQCKAFPHHEFVALIGALLAICCMIFLGFADDVLNLRWRHKLLLPTAASLPLLMVY FTNFGNTTIVVPKPFRPILGLHLDLGILYYVYMGLLAVFCTNAINILAGINGLEAGQSLVISASIIVFNL VELEGDCRDDHVFSLYFMIPFFFTTLGLLYHNWYPSRVFVGDTFCYFAGMTFAVVGILGHFSKTMLLFFM PQVFNFLYSLPQLLHIIPCPRHRIPRLNIKTGKLEMSYSKFKTKSLSFLGTFILKVAESLQLVTVHQSET EDGEFTECNNMTLINLLLKVLGPIHERNLTLLLLLLQILGSAITFSIRYQLVRLFYDV TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001382 |
ORF Size | 1224 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001382.4 |
RefSeq Size | 2159 bp |
RefSeq ORF | 1227 bp |
Locus ID | 1798 |
UniProt ID | Q9H3H5 |
Domains | Glycos_transf_4 |
Protein Families | Transmembrane |
Protein Pathways | Metabolic pathways, N-Glycan biosynthesis |
Gene Summary | The protein encoded by this gene is an enzyme that catalyzes the first step in the dolichol-linked oligosaccharide pathway for glycoprotein biosynthesis. This enzyme belongs to the glycosyltransferase family 4. This protein is an integral membrane protein of the endoplasmic reticulum. The congenital disorder of glycosylation type Ij is caused by mutation in the gene encoding this enzyme. [provided by RefSeq, Jul 2008] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
---|
DPAGT1-CDG: Functional analysis of disease-causing pathogenic mutations and role of endoplasmic reticulum stress
,Yuste-Checa, P;Vega, AI;Martín-Higueras, C;Medrano, C;Gámez, A;Desviat, LR;Ugarte, M;Pérez-Cerdá, C;Pérez, B;,
PLoS ONE
,PubMed ID 28662078
[DPAGT1]
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Documents
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SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC201787 | DPAGT1 (Myc-DDK-tagged)-Human dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) (DPAGT1) |
CNY 3,656.00 |
|
RC201787L1 | Lenti ORF clone of Human dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) (DPAGT1), Myc-DDK-tagged |
CNY 6,056.00 |
|
RC201787L2 | Lenti ORF clone of Human dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) (DPAGT1), mGFP tagged |
CNY 5,890.00 |
|
RC201787L3 | Lenti ORF clone of Human dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) (DPAGT1), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC201787L4 | Lenti ORF clone of Human dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) (DPAGT1), mGFP tagged |
CNY 5,890.00 |
|
SC320266 | DPAGT1 (untagged)-Human dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) (DPAGT1) |
CNY 3,656.00 |