DDAH2 (NM_013974) Human Tagged ORF Clone
CAT#: RG200287
- TrueORF®
DDAH2 (tGFP-tagged) - Human dimethylarginine dimethylaminohydrolase 2 (DDAH2)
ORF Plasmid: DDK
"NM_013974" in other vectors (6)
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CNY 4,370.00
Cited in 1 publication. |
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | DDAH; DDAHII; G6a; HEL-S-277; NG30 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG200287 representing NM_013974
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGACGCCGGGGGAGGGGCTGGGCCGCTGCTCCCATGCCCTGATCCGGGGAGTCCCAGAGAGCCTGG CGTCGGGGGAAGGTGCGGGGGCTGGCCTTCCCGCTCTGGATCTGGCCAAAGCTCAAAGGGAGCACGGGGT GCTGGGAGGTAAACTGAGGCAACGACTGGGGCTACAGCTGCTAGAACTGCCACCTGAGGAGTCATTGCCG CTGGGACCGCTGCTTGGCGACACGGCCGTGATCCAAGGGGACACGGCCCTAATCACGCGGCCCTGGAGCC CCGCTCGTAGGCCAGAGGTCGATGGAGTCCGCAAAGCCCTGCAAGACCTGGGGCTCCGAATTGTGGAAAT AGGAGACGAGAACGCGACGCTGGATGGCACTGACGTTCTCTTCACCGGCCGGGAGTTTTTCGTAGGCCTC TCCAAATGGACCAATCACCGAGGAGCTGAGATCGTGGCGGACACGTTCCGGGACTTCGCCGTCTCCACTG TGCCAGTCTCGGGTCCCTCCCACCTGCGCGGTCTCTGCGGCATGGGGGGACCTCGCACTGTTGTGGCAGG CAGCAGCGACGCTGCCCAAAAGGCTGTCCGGGCAATGGCAGTGCTGACAGATCACCCATATGCCTCCCTG ACCCTCCCAGATGACGCAGCTGCTGACTGTCTCTTTCTTCGTCCTGGGTTGCCTGGTGTGCCCCCTTTCC TCCTGCACCGTGGAGGTGGGGATCTGCCCAACAGCCAGGAGGCACTGCAGAAGCTCTCTGATGTCACCCT GGTACCTGTGTCCTGCTCAGAACTGGAGAAGGCTGGCGCCGGGCTCAGCTCCCTCTGCTTGGTGCTCAGC ACACGCCCCCACAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG200287 representing NM_013974
Red=Cloning site Green=Tags(s) MGTPGEGLGRCSHALIRGVPESLASGEGAGAGLPALDLAKAQREHGVLGGKLRQRLGLQLLELPPEESLP LGPLLGDTAVIQGDTALITRPWSPARRPEVDGVRKALQDLGLRIVEIGDENATLDGTDVLFTGREFFVGL SKWTNHRGAEIVADTFRDFAVSTVPVSGPSHLRGLCGMGGPRTVVAGSSDAAQKAVRAMAVLTDHPYASL TLPDDAAADCLFLRPGLPGVPPFLLHRGGGDLPNSQEALQKLSDVTLVPVSCSELEKAGAGLSSLCLVLS TRPHS TRTRPLE - GFP Tag - V |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_013974 |
ORF Size | 855 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_013974.3 |
RefSeq Size | 1351 bp |
RefSeq ORF | 858 bp |
Locus ID | 23564 |
UniProt ID | O95865 |
Domains | Amidinotransf |
Gene Summary | This gene encodes a dimethylarginine dimethylaminohydrolase. The encoded enzyme functions in nitric oxide generation by regulating the cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. The protein may be localized to the mitochondria. Alternative splicing resulting in multiple transcript variants. [provided by RefSeq, Dec 2014] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Determine exogenous human DDAH2 gene function in rabbit bone marrow-derived endothelial progenitor cells in vitro
,Shoeibi, S;Mohammadi, S;Sadeghnia, HR;Mahdipour, E;Ghayour-Mobarhan, M;,
Cell Biochem. Funct
,PubMed ID 28150318
[DDAH2]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC200287 | DDAH2 (Myc-DDK-tagged)-Human dimethylarginine dimethylaminohydrolase 2 (DDAH2) |
CNY 3,600.00 |
|
RC200287L1 | Lenti ORF clone of Human dimethylarginine dimethylaminohydrolase 2 (DDAH2), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC200287L2 | Lenti ORF clone of Human dimethylarginine dimethylaminohydrolase 2 (DDAH2), mGFP tagged |
CNY 5,890.00 |
|
RC200287L3 | Lenti ORF clone of Human dimethylarginine dimethylaminohydrolase 2 (DDAH2), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC200287L4 | Lenti ORF clone of Human dimethylarginine dimethylaminohydrolase 2 (DDAH2), mGFP tagged |
CNY 5,890.00 |
|
SC319363 | DDAH2 (untagged)-Human dimethylarginine dimethylaminohydrolase 2 (DDAH2) |
CNY 3,600.00 |