EMSY (NM_001300942) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	C11orf30; GL002
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC240114 representing NM_001300942 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTGTTGTGTGGCCAACCCTTCTGGATCTCAGCAGGGATGAATGCAAAAGAATTCTTCGAAAATTGG AATTGGAGGCATATGCTGGAGTTATCAGTGCACTTCGGGCACAGGGGGATCTCACCAAGGAAAAGAAAGA TCTTCTTGGAGAACTATCAAAAGTTCTTAGCATCTCAACAGAACGCCACCGTGCTGAAGTTCGGAGAGCA GTAAACGATGAACGGTTAACAACAATTGCACATAAAATGAATTTATCCTTATATTTGGGTGAAAGACCAA GTTACAGTATGTCTGGACCTAATAGCTCTTCAGAATGGTCCATTGAAGGTCGTCGATTGGTACCACTGAT GCCCCGGCTCGTTCCCCAAACCGCCTTTACTGTAACAGCTAATGCTGTTGCTAATGCAGCTATCCAGCAT AATGCATCTCTTCCAGTGCCTGCAGAAACAGGAAGCAAGGAAGTAGTGGTTTGCTATTCCTACACAAGTA CCACGTCAACCCCAACCTCTACCCCTGTTCCAAGTGGCAGCATAGCAACGGTTAAGTCTCCAAGACCTGC CAGTCCTGCCTCCAATGTAGTTGTCTTGCCAAGTGGAAGTACTGTTTATGTCAAAAGTGTAAGCTGTTCA GATGAAGATGAAAAACCCAGAAAACGAAGGCGAACAAACTCTTCCAGCTCCTCTCCTGTTGTTCTAAAGG AAGTTCCAAAGGCCGTTGTTCCAGTCTCAAAGACGATCACTGTGCCTGTGAGTGGTAGTCCCAAGATGAG CAACATCATGCAGAGCATTGCCAACTCCTTACCACCCCACATGTCTCCTGTAAAAATAACCTTCACTAAA CCATCAACACAGACAACAAACACAACAACACAGAAGGTTATTATAGTCACCACATCACCAAGCTCAACCT TCGTGCCCAACATTCTCTCCAAATCCCATAACTATGCAGCAGTCACTAAGCTTGTACCAACGTCAGTCAT TGCTTCTACAACCCAGAAGCCACCAGTTGTTATAACTGCTTCACAGTCCTCTCTGGTCAGTAATAGCAGC AGTGGCAGCAGCAGTTCTACACCATCACCTATTCCTAATACAGTTGCAGTAACAGCTGTGGTGTCCTCTA CACCATCTGTGGTCATGTCAACAGTAGCACAAGGTGTATCTACATCAGCAATCAAAATGGCATCAACCAG ACTTCCTTCCCCCAAAAGCTTAGTGAGTGCCCCAACTCAGATTCTTGCACAGTTCCCTAAACAACATCAA CAGTCTCCTAAGCAGCAGTTATATCAAGTGCAACAGCAGACACAGCAACAAGTGGCCCAGCCTTCTCCAG TATCTCATCAGCAACAGCCTCAGCAGTCTCCTTTGCCACCTGGTATTAAACCTACCATCCAAATCAAACA GGAGTCAGGTGTTAAAATCATCACACAACAGGTTCAACCAAGTAAAATCTTACCCAAACCAGTGACAGCA ACTCTACCCACCAGTAGCAATTCCCCTATTATGGTGGTTAGCAGTAATGGTGCAATTATGACAACTAAAC TGGTAACCACTCCTACTGGCACACAAGCAACCTATACCCGGCCAACAGTGAGCCCATCCATTGGTCGGAT GGCTGCAACCCCTGGAGCTGCAACCTATGTGAAAACTACGAGTGGTAGCATCATTACAGTAGTACCCAAA TCATTAGCTACCTTGGGGGGCAAGATAATTAGCAGTAATATAGTTTCTGGAACGACTACCAAAATCACTA CAATCCCAATGACTTCCAAGCCCAACGTGATTGTTGTACAAAAGACTACAGGAAAAGGAACGACCATTCA AGGCCTCCCGGGCAAAAATGTTGTCACAACGTTGCTAAATGCTGGAGGAGAAAAGACTATTCAGACAGTG CCAACAGGAGCAAAGCCAGCTATCCTTACTGCTACAAGACCCATCACCAAAATGATTGTAACGCAGCCAA AAGGAATAGGTTCTACAGTTCAACCAGCAGCTAAAATCATCCCAACAAAAATTGTTTATGGGCAGCAAGG GAAAACGCAGGTTCTTATTAAACCCAAACCAGTGACTTTTCAAGCGACAGTTGTTAGTGAACAAACAAGA CAGCTAGTAACAGAAACATTACAGCAAGCATCCAGGGTAGCAGAGGCTGGTAATTCATCTATTCAGGAAG GAAAAGAAGAACCACAGAATTATACAGATAGTAGTTCCTCTTCTACAGAGTCCTCCCAGAGTTCCCAAGA TTCCCAGCCTGTAGTTCATGTAATTGCTTCCCGGCGTCAGGATTGGTCAGAACATGAGATTGCAATGGAG ACTAGCCCTACCATAATTTATCAGGATGTATCCAGTGAATCACAATCAGCTACTTCAACAATCAAAGCTC TGTTAGAACTCCAACAGACAACAGTAAAGGAAAAATTGGAATCTAAACCAAGACAACCCACTATTGACCT GAGTCAAATGGCAGTGCCTATTCAGATGACCCAGGAAAAGAGACATTCTCCTGAGAGTCCATCAATTGCT GTGGTAGAGTCAGAACTAGTAGCTGAATACATCACTACTGAACGCACTGATGAGGGGACAGAGGTTGCTT TTCCCCTTCTAGTCAGCCATCGCTCCCAGCCCCAACAGCCTTCCCAGCCCCAGCGGACCCTGCTCCAGCA TGTGGCTCAGTCACAGACCGCAACACAGACTTCGGTGGTGGTGAAGTCCATCCCAGCATCTTCCCCTGGA GCAATCACCCACATTATGCAGCAGGCATTAAGCAGTCACACTGCTTTTACCAAACACAGCGAGGAACTTG GAACTGAGGAGGGCGAGGTTGAAGAGATGGACACTTTAGACCCTCAGACAGGTCTGTTTTACCGATCTGC CCTGACTCAGTCACAGTCAGCTAAACAGCAGAAACTTAGCCAGCCCCCGCTGGAACAGACTCAGCTGCAA GTGAAAACTCTGCAGTGCTTCCAGACTAAACAGAAGCAGACCATCCACCTGCAGGCAGACCAGCTCCAGC ACAAACTCCCGCAAATGCCCCAGCTTTCCATCAGGCATCAAAAACTCACCCCTCTCCAGCAAGAACAAGC ACAGCCCAAGCCAGATGTACAGCACACACAGCATCCCATGGTGGCCAAAGACAGGCAGCTTCCTACCTTA ATGGCACAGCCCCCGCAAACTGTAGTACAGGTGCTTGCAGTGAAAACCACGCAGCAGCTCCCTAAACTGC AGCAGGCTCCGAACCAACCAAAAATCTACGTGCAACCCCAAACCCCCCAGAGCCAAATGTCGCTCCCAGC TTCTTCAGAGAAACAGACGGCAAGCCAGGTGGAGCAGCCAATTATAACCCAAGGATCCTCTGTTACAAAG ATAACTTTTGAGGGGCGCCAGCCTCCCACAGTTACAAAGATAACTGGTGGCAGTTCTGTGCCTAAGCTGA CATCACCAGTTACAAGCATATCTCCCATTCAGGCCTCTGAGAAGACAGCAGTGTCTGACATTTTGAAAAT GTCTTTGATGGAAGCTCAGATTGATACAAATGTAGAACATATGATAGTGGATCCCCCAAAGAAGGCTCTT GCCACTAGCATGCTCACTGGTGAAGCAGGATCATTACCCTCCACCCACATGGTGGTGGCAGGGATGGCGA ATTCCACTCCCCAGCAACAGAAATGTAGAGAGTCCTGTTCGAGTCCATCCACTGTTGGCTCTTCCCTAAC GACAAGGAAAATTGATCCACCAGCAGTGCCTGCGACAGGCCAGTTCATGCGTATTCAGAATGTAGGCCAA AAGAAAGCTGAAGAGAGTCCAGCAGAAATTATCATCCAGGCTATTCCTCAGTATGCTATTCCTTGTCACT CCAGCTCCAATGTGGTGGTGGAGCCCAGTGGGCTTCTTGAGCTAAACAACTTCACTAGTCAACAGCTGGA TGATGAGGAGACAGCAATGGAGCAGGACATAGACAGTAGCACGGAGGATGGAACTGAACCCAGCCCTTCT CAGAGCTCTGCTGAACGGTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC240114 representing NM_001300942 Red=Cloning site Green=Tags(s) MPVVWPTLLDLSRDECKRILRKLELEAYAGVISALRAQGDLTKEKKDLLGELSKVLSISTERHRAEVRRA VNDERLTTIAHKMNLSLYLGERPSYSMSGPNSSSEWSIEGRRLVPLMPRLVPQTAFTVTANAVANAAIQH NASLPVPAETGSKEVVVCYSYTSTTSTPTSTPVPSGSIATVKSPRPASPASNVVVLPSGSTVYVKSVSCS DEDEKPRKRRRTNSSSSSPVVLKEVPKAVVPVSKTITVPVSGSPKMSNIMQSIANSLPPHMSPVKITFTK PSTQTTNTTTQKVIIVTTSPSSTFVPNILSKSHNYAAVTKLVPTSVIASTTQKPPVVITASQSSLVSNSS SGSSSSTPSPIPNTVAVTAVVSSTPSVVMSTVAQGVSTSAIKMASTRLPSPKSLVSAPTQILAQFPKQHQ QSPKQQLYQVQQQTQQQVAQPSPVSHQQQPQQSPLPPGIKPTIQIKQESGVKIITQQVQPSKILPKPVTA TLPTSSNSPIMVVSSNGAIMTTKLVTTPTGTQATYTRPTVSPSIGRMAATPGAATYVKTTSGSIITVVPK SLATLGGKIISSNIVSGTTTKITTIPMTSKPNVIVVQKTTGKGTTIQGLPGKNVVTTLLNAGGEKTIQTV PTGAKPAILTATRPITKMIVTQPKGIGSTVQPAAKIIPTKIVYGQQGKTQVLIKPKPVTFQATVVSEQTR QLVTETLQQASRVAEAGNSSIQEGKEEPQNYTDSSSSSTESSQSSQDSQPVVHVIASRRQDWSEHEIAME TSPTIIYQDVSSESQSATSTIKALLELQQTTVKEKLESKPRQPTIDLSQMAVPIQMTQEKRHSPESPSIA VVESELVAEYITTERTDEGTEVAFPLLVSHRSQPQQPSQPQRTLLQHVAQSQTATQTSVVVKSIPASSPG AITHIMQQALSSHTAFTKHSEELGTEEGEVEEMDTLDPQTGLFYRSALTQSQSAKQQKLSQPPLEQTQLQ VKTLQCFQTKQKQTIHLQADQLQHKLPQMPQLSIRHQKLTPLQQEQAQPKPDVQHTQHPMVAKDRQLPTL MAQPPQTVVQVLAVKTTQQLPKLQQAPNQPKIYVQPQTPQSQMSLPASSEKQTASQVEQPIITQGSSVTK ITFEGRQPPTVTKITGGSSVPKLTSPVTSISPIQASEKTAVSDILKMSLMEAQIDTNVEHMIVDPPKKAL ATSMLTGEAGSLPSTHMVVAGMANSTPQQQKCRESCSSPSTVGSSLTTRKIDPPAVPATGQFMRIQNVGQ KKAEESPAEIIIQAIPQYAIPCHSSSNVVVEPSGLLELNNFTSQQLDDEETAMEQDIDSSTEDGTEPSPS QSSAERS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001300942
ORF Size	4011 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001300942.2
RefSeq Size	6916 bp
RefSeq ORF	4014 bp
Locus ID	56946
UniProt ID	Q7Z589
MW	143.6 kDa
Gene Summary	Regulator which is able to repress transcription, possibly via its interaction with a multiprotein chromatin remodeling complex that modifies the chromatin. Its interaction with BRCA2 suggests that it may play a central role in the DNA repair function of BRCA2. As part of a histone H3-specific methyltransferase complex may mediate ligand-dependent transcriptional activation by nuclear hormone receptors.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.