PSD93 (DLG2) (NM_001300983) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	chapsyn-110; PPP1R58; PSD-93; PSD93
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239685 representing NM_001300983 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCTTTGCATGTTACTGTGCACTCCGGACTAACGTGAAGAAGTATCGATATCAAGATGAGGACGCTC CACATGATCATTCCTTACCTCGACTAACCCACGAAGTAAGAGGCCCAGAACTCGTGCATGTATCAGAAAA GAACCTCTCTCAAATAGAAAATGTCCATGGATATGTCTTGCAGTCTCATATTTCTCCTCTGAAGGCCAGT CCTGCTCCTATAATTGTCAACACAGATACTTTGGACACAATTCCTTATGTCAATGGGACAGAAATTGAAT ATGAATTTGAAGAAATTACACTGGAGAGGGGGAATTCTGGCCTGGGATTCAGTATTGCTGGGGGGACAGA TAATCCCCACATTGGAGATGACCCTGGCATATTTATTACGAAGATTATACCAGGAGGTGCTGCAGCAGAG GATGGCAGACTCAGGGTCAATGATTGTATCTTGCGGGTGAATGAGGTTGATGTGTCAGAGGTTTCCCACA GTAAAGCGGTGGAAGCCCTGAAGGAAGCAGGGTCTATCGTTCGGCTGTATGTGCGTAGAAGACGACCTAT TTTGGAGACCGTTGTGGAAATCAAACTGTTCAAAGGCCCTAAAGGTTTAGGCTTCAGTATTGCAGGAGGT GTGGGGAACCAACACATTCCTGGAGACAACAGCATTTATGTAACTAAAATTATAGATGGAGGAGCTGCAC AAAAAGATGGAAGGTTGCAAGTAGGAGATAGACTACTAATGGTAAACAACTACAGTTTAGAAGAAGTAAC ACACGAAGAGGCAGTAGCAATATTAAAGAACACATCAGAGGTAGTTTATTTAAAAGTTGGCAAACCCACT ACCATTTATATGACTGATCCTTATGGTCCACCTGATATTACTCACTCTTATTCTCCACCAATGGAAAACC ATCTACTCTCTGGCAACAATGGCACTTTAGAATATAAAACCTCCCTGCCACCCATCTCTCCAGGAAGGTA CTCACCAATTCCAAAGCACATGCTTGTTGACGACGACTACACCAGGCCTCCGGAACCTGTTTACAGCACT GTGAACAAACTATGTGATAAGCCTGCTTCTCCCAGGCACTATTCCCCTGTTGAGTGTGACAAAAGCTTCC TCCTCTCAGCTCCCTATTCCCACTACCACCTAGGCCTGCTACCTGACTCTGAGATGACCAGTCATTCCCA ACATAGCACCGCAACTCGTCAGCCTTCAATGACTCTCCAACGGGCCGTCTCCCTGGAAGGAGAGCCTCGC AAGGTAGTCCTGCACAAAGGCTCCACTGGCCTGGGCTTCAACATTGTCGGTGGGGAAGATGGAGAAGGTA TTTTTGTGTCCTTCATTCTGGCTGGTGGACCAGCAGACCTAAGTGGGGAGCTCCAGAGAGGAGACCAGAT CCTATCGGTGAATGGCATTGACCTCCGTGGTGCATCCCACGAGCAGGCAGCTGCTGCACTAAAGGGGGCT GGACAGACAGTGACGATTATAGCACAATATCAACCTGAAGATTACGCTCGATTTGAGGCCAAAATCCATG ACCTACGAGAGCAGATGATGAACCACAGCATGAGCTCCGGGTCCGGATCCCTGCGAACCAATCAGAAACG CTCCCTCTACGTCAGAGCCATGTTCGACTACGACAAGAGCAAGGACAGTGGGCTGCCAAGTCAAGGACTT AGTTTTAAATATGGAGATATTCTCCACGTTATCAATGCCTCTGATGATGAGTGGTGGCAAGCCAGGAGAG TCATGCTGGAGGGAGACAGTGAGGAGATGGGGGTCATCCCCAGCAAAAGGAGGGTGGAAAGAAAGGAACG TGCCCGATTGAAGACAGTGAAGTTTAATGCCAAACCTGGAGTGATTGATTCGAAAGGGGACATCCCCGGA TTAGGTGACGACGGTTATGGAACAAAGACTCTGAGAGGACAAGAAGACCTCATTCTTTCCTATGAGCCTG TTACAAGGCAGGAAATAAACTACACCCGGCCGGTGATTATCCTGGGGCCCATGAAGGATCGGATCAATGA CGACTTGATATCTGAATTCCCTGATAAATTTGGCTCCTGTGTGCCTCATACTACGAGGCCAAAGCGAGAC TACGAGGTGGATGGCAGAGACTATCACTTTGTCATTTCCAGAGAACAAATGGAGAAAGATATCCAAGAGC ACAAGTTTATAGAAGCCGGCCAGTACAATGACAATTTATATGGAACCAGTGTGCAGTCTGTGAGATTTGT AGCAGAAAGAGGCAAACACTGTATACTTGATGTATCAGGAAATGCTATCAAGCGGTTACAAGTTGCCCAG CTCTATCCCATTGCCATCTTCATAAAACCCAGGTCTCTGGAACCTCTTATGGAGATGAATAAGCGTCTAA CAGAGGAACAAGCCAAGAAAACCTATGATCGAGCAATTAAGCTAGAACAAGAATTTGGAGAATATTTTAC AGCTATTGTCCAAGGAGATACTTTAGAAGATATATATAACCAATGCAAGCTTGTTATTGAAGAGCAATCT GGGCCTTTCATCTGGATTCCCTCAAAGGAAAAGTTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239685 representing NM_001300983 Red=Cloning site Green=Tags(s) MFFACYCALRTNVKKYRYQDEDAPHDHSLPRLTHEVRGPELVHVSEKNLSQIENVHGYVLQSHISPLKAS PAPIIVNTDTLDTIPYVNGTEIEYEFEEITLERGNSGLGFSIAGGTDNPHIGDDPGIFITKIIPGGAAAE DGRLRVNDCILRVNEVDVSEVSHSKAVEALKEAGSIVRLYVRRRRPILETVVEIKLFKGPKGLGFSIAGG VGNQHIPGDNSIYVTKIIDGGAAQKDGRLQVGDRLLMVNNYSLEEVTHEEAVAILKNTSEVVYLKVGKPT TIYMTDPYGPPDITHSYSPPMENHLLSGNNGTLEYKTSLPPISPGRYSPIPKHMLVDDDYTRPPEPVYST VNKLCDKPASPRHYSPVECDKSFLLSAPYSHYHLGLLPDSEMTSHSQHSTATRQPSMTLQRAVSLEGEPR KVVLHKGSTGLGFNIVGGEDGEGIFVSFILAGGPADLSGELQRGDQILSVNGIDLRGASHEQAAAALKGA GQTVTIIAQYQPEDYARFEAKIHDLREQMMNHSMSSGSGSLRTNQKRSLYVRAMFDYDKSKDSGLPSQGL SFKYGDILHVINASDDEWWQARRVMLEGDSEEMGVIPSKRRVERKERARLKTVKFNAKPGVIDSKGDIPG LGDDGYGTKTLRGQEDLILSYEPVTRQEINYTRPVIILGPMKDRINDDLISEFPDKFGSCVPHTTRPKRD YEVDGRDYHFVISREQMEKDIQEHKFIEAGQYNDNLYGTSVQSVRFVAERGKHCILDVSGNAIKRLQVAQ LYPIAIFIKPRSLEPLMEMNKRLTEEQAKKTYDRAIKLEQEFGEYFTAIVQGDTLEDIYNQCKLVIEEQS GPFIWIPSKEKL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001300983
ORF Size	2556 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001300983.1, NP_001287912.1
RefSeq Size	7668 bp
RefSeq ORF	2559 bp
Locus ID	1740
Protein Families	Druggable Genome
MW	95.5 kDa
Gene Summary	This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family. The encoded protein forms a heterodimer with a related family member that may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. Multiple transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described, but their full-length nature is not known. [provided by RefSeq, Dec 2008]

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