LAMA3 (NM_001302996) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	BM600; E170; LAMNA; LOCS
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238518 representing NM_001302996 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGCCGCGCGGCCTCGGGGTCGGGCACTGGGGCCAGTACTGCCGCCGACGCCGCTGCTCCTGC TGGTACTGCGGGTGCTGCCAGCCTGCGGGGCGACCGCTCGGGATCCCGGGGCCGCGGCCGGGCTCAGCCT TCACCCGACTTACTTCAACCTGGCCGAGGCGGCGAGGATTTGGGCCACCGCCACCTGCGGGGAGAGGGGA CCCGGCGAGGGGAGGCCCCAGCCCGAGCTCTACTGCAAGTTGGTCGGGGGCCCCACCGCCCCAGGCAGCG GCCACACCATCCAGGGCCAGTTCTGTGACTATTGCAATTCTGAAGACCCCAGGAAAGCACATCCTGTCAC CAATGCCATCGATGGATCTGAACGTTGGTGGCAAAGCCCTCCCCTGTCCTCAGGCACACAGTACAACAGA GTCAACCTCACCTTGGATCTGGGGCAGCTCTTCCATGTGGCCTATATTTTAATCAAATTTGCAAATTCTC CTCGCCCTGATCTTTGGGTCTTGGAAAGATCTGTAGACTTTGGAAGCACCTACTCACCATGGCAATATTT TGCTCATTCTAAAGTAGACTGTTTAAAAGAATTTGGGCGGGAGGCAAATATGGCTGTCACCCGGGATGAT GATGTACTTTGTGTTACTGAATATTCCCGTATTGTACCTTTGGAAAATGGTGAGGTTGTGGTGTCCTTGA TAAACGGTCGTCCAGGTGCAAAAAATTTTACTTTCTCTCACACCCTGAGGGAGTTTACCAAGGCAACAAA CATCCGCTTGCGTTTTCTTAGAACCAATACGCTTCTTGGACACCTCATCTCCAAAGCCCAGCGAGATCCA ACTGTCACTCGGCGGTATTATTACAGCATAAAGGACATCAGCATTGGTGGGCAGTGTGTTTGCAATGGCC ATGCTGAAGTGTGCAATATAAACAATCCTGAAAAACTGTTTCGGTGTGAATGCCAGCACCACACCTGTGG GGAGACGTGTGATCGCTGCTGCACAGGGTACAATCAGAGGCGCTGGCGGCCCGCCGCTTGGGAGCAGAGC CACGAGTGTGAAGCATGCAACTGCCACGGCCATGCCAGCAACTGTTACTATGATCCAGATGTTGAGCGGC AGCAGGCAAGCTTGAATACCCAGGGCATCTATGCTGGTGGAGGGGTCTGCATTAACTGTCAGCACAACAC AGCTGGAGTAAACTGTGAACAGTGTGCTAAGGGCTATTACCGCCCTTATGGGGTTCCAGTGGATGCCCCT GATGGCTGCATCCGTAAGTTTCATTTCAAGTTGGTGTATCTTAGCCTGTGTGTACTGCCTCAGCGAAGTC ATCAGGCTAACTTTGGATCAGTTAATAACTTCCTTCATGCCCTCTCTCTTCAGAGTATTAGTTGTGCTCG CTATGTGACCTCAGTCACTTACACAGTCTCTTTGAATTTTGGATTTATTGCATGTAAATGGAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238518 representing NM_001302996 Red=Cloning site Green=Tags(s) MAAAARPRGRALGPVLPPTPLLLLVLRVLPACGATARDPGAAAGLSLHPTYFNLAEAARIWATATCGERG PGEGRPQPELYCKLVGGPTAPGSGHTIQGQFCDYCNSEDPRKAHPVTNAIDGSERWWQSPPLSSGTQYNR VNLTLDLGQLFHVAYILIKFANSPRPDLWVLERSVDFGSTYSPWQYFAHSKVDCLKEFGREANMAVTRDD DVLCVTEYSRIVPLENGEVVVSLINGRPGAKNFTFSHTLREFTKATNIRLRFLRTNTLLGHLISKAQRDP TVTRRYYYSIKDISIGGQCVCNGHAEVCNINNPEKLFRCECQHHTCGETCDRCCTGYNQRRWRPAAWEQS HECEACNCHGHASNCYYDPDVERQQASLNTQGIYAGGGVCINCQHNTAGVNCEQCAKGYYRPYGVPVDAP DGCIRKFHFKLVYLSLCVLPQRSHQANFGSVNNFLHALSLQSISCARYVTSVTYTVSLNFGFIACKWK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001302996
ORF Size	1464 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001302996.2
RefSeq Size	1749 bp
RefSeq ORF	1467 bp
Locus ID	3909
UniProt ID	Q16787
Protein Families	Druggable Genome, Secreted Protein
Protein Pathways	ECM-receptor interaction, Focal adhesion, Pathways in cancer, Small cell lung cancer
MW	54.6 kDa
Gene Summary	The protein encoded by this gene belongs to the laminin family of secreted molecules. Laminins are heterotrimeric molecules that consist of alpha, beta, and gamma subunits that assemble through a coiled-coil domain. Laminins are essential for formation and function of the basement membrane and have additional functions in regulating cell migration and mechanical signal transduction. This gene encodes an alpha subunit and is responsive to several epithelial-mesenchymal regulators including keratinocyte growth factor, epidermal growth factor and insulin-like growth factor. Mutations in this gene have been identified as the cause of Herlitz type junctional epidermolysis bullosa and laryngoonychocutaneous syndrome. Alternative splicing and alternative promoter usage result in multiple transcript variants. [provided by RefSeq, Dec 2014]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.