Heme oxygenase 2 (HMOX2) (NM_001286267) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	HO-2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC237781 representing NM_001286267 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCTCGCTCTGTTGCCCAGGCTGGAGTGCAGTGGATCAGTCTAGCTCATTGCAGCCTCCACCTCC TGGGTTCAAGAGATTCTCCTGCCTCAGCCTCCTGTGTAGCTGGGATTACAGGACCAGAGGAGCGAGAGCA GCAAGAACCACACCCAGCAGCAATGTCAGCGGAAGTGGAAACCTCAGAGGGGGTAGACGAGTCAGAAAAA AAGAACTCTGGGGCCCTAGAAAAGGAGAACCAAATGAGAATGGCTGACCTCTCGGAGCTCCTGAAGGAAG GGACCAAGGAAGCACACGACCGGGCAGAAAACACCCAGTTTGTCAAGGACTTCTTGAAAGGCAACATTAA GAAGGAGCTGTTTAAGCTGGCCACCACGGCACTTTACTTCACATACTCAGCCCTCGAGGAGGAAATGGAG CGCAACAAGGACCATCCAGCCTTTGCCCCTTTGTACTTCCCCATGGAGCTGCACCGGAAGGAGGCGCTGA CCAAGGACATGGAGTATTTCTTTGGTGAAAACTGGGAGGAGCAGGTGCAGTGCCCCAAGGCTGCCCAGAA GTACGTGGAGCGGATCCACTACATAGGGCAGAACGAGCCGGAGCTACTGGTGGCCCATGCATACACCCGC TACATGGGGGATCTCTCGGGGGGCCAGGTGCTGAAGAAGGTGGCCCAGCGAGCACTGAAACTCCCCAGCA CAGGGGAAGGGACCCAGTTCTACCTGTTTGAGAATGTGGACAATGCCCAGCAGTTCAAGCAGCTCTACCG GGCCAGGATGAACGCCCTGGACCTGAACATGAAGACCAAAGAGAGGATCGTGGAGGAGGCCAACAAGGCT TTTGAGTATAACATGCAGATATTCAATGAACTGGACCAGGCCGGCTCCACACTGGCCAGAGAGACCTTGG AGGATGGGTTCCCTGTACACGATGGGAAAGGAGACATGCGTAAATGCCCTTTCTACGCTGCTGAACAAGA CAAAGGTGCCCTGGAGGGCAGCAGCTGTCCCTTCCGAACAGCTATGGCTGTGCTGAGGAAGCCCAGCCTC CAGTTCATCCTGGCCGCTGGTGTGGCCCTAGCTGCTGGACTCTTGGCCTGGTACTACATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC237781 representing NM_001286267 Red=Cloning site Green=Tags(s) MESRSVAQAGVQWISLAHCSLHLLGSRDSPASASCVAGITGPEEREQQEPHPAAMSAEVETSEGVDESEK KNSGALEKENQMRMADLSELLKEGTKEAHDRAENTQFVKDFLKGNIKKELFKLATTALYFTYSALEEEME RNKDHPAFAPLYFPMELHRKEALTKDMEYFFGENWEEQVQCPKAAQKYVERIHYIGQNEPELLVAHAYTR YMGDLSGGQVLKKVAQRALKLPSTGEGTQFYLFENVDNAQQFKQLYRARMNALDLNMKTKERIVEEANKA FEYNMQIFNELDQAGSTLARETLEDGFPVHDGKGDMRKCPFYAAEQDKGALEGSSCPFRTAMAVLRKPSL QFILAAGVALAAGLLAWYYM TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286267
ORF Size	1110 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286267.1, NP_001273196.1
RefSeq Size	1875 bp
RefSeq ORF	1113 bp
Locus ID	3163
UniProt ID	P30519
Protein Families	Transmembrane
Protein Pathways	Porphyrin and chlorophyll metabolism
MW	42.1 kDa
Gene Summary	Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. Several alternatively spliced transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Oct 2013]

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