CACNA1B (NM_001243812) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	BIII; CACNL1A5; CACNN; Cav2.2; DYT23; NEDNEH
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC235372 representing NM_001243812 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCCGCTTCGGGGACGAGCTGGGCGGCCGCTATGGGGGCCCCGGCGGCGGAGAGCGGGCCCGGGGCG GCGGGGCCGGCGGGGCGGGGGGCCCGGGTCCCGGGGGGCTGCAGCCCGGCCAGCGGGTCCTCTACAAGCA ATCGATCGCGCAGCGCGCGCGGACCATGGCGCTGTACAACCCCATCCCGGTCAAGCAGAACTGCTTCACC GTCAACCGCTCGCTCTTCGTCTTCAGCGAGGACAACGTCGTCCGCAAATACGCGAAGCGCATCACCGAGT GGCCTCCATTCGAGTATATGATCCTGGCCACCATCATCGCCAACTGCATCGTGCTGGCCCTGGAGCAGCA CCTCCCTGATGGGGACAAAACGCCCATGTCCGAGCGGCTGGACGACACGGAGCCCTATTTCATCGGGATC TTTTGCTTCGAGGCAGGGATCAAAATCATCGCTCTGGGCTTTGTCTTCCACAAGGGCTCTTACCTGCGGA ACGGCTGGAACGTCATGGACTTCGTGGTCGTCCTCACAGGGATCCTTGCCACGGCTGGAACTGACTTCGA CCTGCGAACACTGAGGGCTGTGCGTGTGCTGAGGCCCCTGAAGCTGGTGTCTGGGATTCCAAGTTTGCAG GTGGTGCTCAAGTCCATCATGAAGGCCATGGTTCCACTCCTGCAGATTGGGCTGCTTCTCTTCTTTGCCA TCCTCATGTTTGCCATCATTGGCCTGGAGTTCTACATGGGCAAGTTCCACAAGGCCTGTTTCCCCAACAG CACAGATGCGGAGCCCGTGGGTGACTTCCCCTGTGGCAAGGAGGCCCCAGCCCGGCTGTGCGAGGGCGAC ACTGAGTGCCGGGAGTACTGGCCAGGACCCAACTTTGGCATCACCAACTTTGACAATATCCTGTTTGCCA TCTTGACGGTGTTCCAGTGCATCACCATGGAGGGCTGGACTGACATCCTCTATAATACAAACGATGCGGC CGGCAACACCTGGAACTGGCTCTACTTCATCCCTCTCATCATCATCGGCTCCTTCTTCATGCTCAACCTG GTGCTGGGCGTGCTCTCGGGGGAGTTTGCCAAGGAGCGAGAGAGGGTGGAGAACCGCCGCGCCTTCCTGA AGCTGCGCCGGCAGCAGCAGATCGAGCGAGAGCTCAACGGGTACCTGGAGTGGATCTTCAAGGCGGAGGA AGTCATGCTGGCCGAGGAGGACAGGAATGCAGAGGAGAAGTCCCCTTTGGACGTGCTGAAGAGAGCGGCC ACCAAGAAGAGCAGAAATGACCTGATCCACGCAGAGGAGGGAGAGGACCGGTTTGCAGATCTCTGTGCTG TTGGATCCCCCTTCGCCCGCGCCAGCCTCAAGAGCGGGAAGACAGAGAGCTCGTCATACTTCCGGAGGAA GGAGAAGATGTTCCGGTTTTTTATCCGGCGCATGGTGAAGGCTCAGAGCTTCTACTGGGTGGTGCTGTGC GTGGTGGCCCTGAACACACTGTGTGTGGCCATGGTGCATTACAACCAGCCGCGGCGGCTTACCACGACCC TGTATTTTGCAGAGTTTGTTTTCCTGGGTCTCTTCCTCACAGAGATGTCCCTGAAGATGTATGGCCTGGG GCCCAGAAGCTACTTCCGGTCCTCCTTCAACTGCTTCGACTTTGGGGTCATCGTGGGGAGCGTCTTTGAA GTGGTCTGGGCGGCCATCAAGCCGGGAAGCTCCTTTGGGATCAGTGTGCTGCGGGCCCTCCGCCTGCTGA GGATCTTCAAAGTCACGAAGTACTGGAGCTCCCTGCGGAACCTGGTGGTGTCCCTGCTGAACTCCATGAA GTCCATCATCAGCCTGCTCTTCTTGCTCTTCCTGTTCATTGTGGTCTTCGCCCTGCTGGGGATGCAGCTG TTTGGGGGACAGTTCAACTTCCAGGATGAGACTCCCACAACCAACTTCGACACCTTCCCTGCCGCCATCC TCACTGTCTTCCAGATCCTGACGGGAGAGGACTGGAATGCAGTGATGTATCACGGGATCGAATCGCAAGG CGGCGTCAGCAAAGGCATGTTCTCGTCCTTTTACTTCATTGTCCTGACACTGTTCGGAAACTACACTCTG CTGAATGTCTTTCTGGCCATCGCTGTGGACAACCTGGCCAACGCCCAAGAGCTGACCAAGGATGAAGAGG AGATGGAAGAAGCAGCCAATCAGAAGCTTGCTCTGCAAAAGGCCAAAGAAGTGGCTGAAGTCAGCCCCAT GTCTGCCGCGAACATCTCCATCGCCGCCAGGCAGCAGAACTCGGCCAAGGCGCGCTCGGTGTGGGAGCAG CGGGCCAGCCAGCTACGGCTGCAGAACCTGCGGGCCAGCTGCGAGGCGCTGTACAGCGAGATGGACCCCG AGGAGCGGCTGCGCTTCGCCACTACGCGCCACCTGCGGCCCGACATGAAGACGCACCTGGACCGGCCGCT GGTGGTGGAGCTGGGCCGCGACGGCGCGCGGGGGCCCGTGGGAGGCAAAGCCCGACCTGAGGCTGCGGAG GCCCCCGAGGGCGTCGACCCTCCGCGCAGGCACCACCGGCACCGCGACAAGGACAAGACCCCCGCGGCGG GGGACCAGGACCGAGCAGAGGCCCCGAAGGCGGAGAGCGGGGAGCCCGGTGCCCGGGAGGAGCGGCCGCG GCCGCACCGCAGCCACAGCAAGGAGGCCGCGGGGCCCCCGGAGGCGCGGAGCGAGCGCGGCCGAGGCCCA GGCCCCGAGGGCGGCCGGCGGCACCACCGGCGCGGCTCCCCGGAGGAGGCGGCCGAGCGGGAGCCCCGAC GCCACCGCGCGCACCGGCACCAGGATCCGAGCAAGGAGTGCGCCGGCGCCAAGGGCGAGCGGCGCGCGCG GCACCGCGGCGGCCCCCGAGCGGGGCCCCGGGAGGCGGAGAGCGGGGAGGAGCCGGCGCGGCGGCACCGG GCCCGGCACAAGGCGCAGCCTGCTCACGAGGCTGTGGAGAAGGAGACCACGGAGAAGGAGGCCACGGAGA AGGAGGCTGAGATAGTGGAAGCCGACAAGGAAAAGGAGCTCCGGAACCACCAGCCCCGGGAGCCACACTG TGACCTGGAGACCAGTGGGACTGTGACTGTGGGTCCCATGCACACACTGCCCAGCACCTGTCTCCAGAAG GTGGAGGAACAGCCAGAGGATGCAGACAATCAGCGGAACGTCACTCGCATGGGCAGTCAGCCCCCAGACC CGAACACTATTGTACATATCCCAGTGATGCTGACGGGCCCTCTTGGGGAAGCCACGGTCGTTCCCAGTGG TAACGTGGACCTGGAAAGCCAAGCAGAGGGGAAGAAGGAGGTGGAAGCGGATGACGTGATGAGGAGCGGC CCCCGGCCTATCGTCCCATACAGCTCCATGTTCTGTTTAAGCCCCACCAACCTGCTCCGCCGCTTCTGCC ACTACATCGTGACCATGAGGTACTTCGAGGTGGTCATTCTCGTGGTCATCGCCTTGAGCAGCATCGCCCT GGCTGCTGAGGACCCAGTGCGCACAGACTCGCCCAGGAACAACGCTCTGAAATACCTGGATTACATTTTC ACTGGTGTCTTTACCTTTGAGATGGTGATAAAGATGATCGACTTGGGACTGCTGCTTCACCCTGGAGCCT ATTTCCGGGACTTGTGGAACATTCTGGACTTCATTGTGGTCAGTGGCGCCCTGGTGGCGTTTGCTTTCTC AGGATCCAAAGGGAAAGACATCAATACCATCAAGTCTCTGAGAGTCCTTCGTGTCCTGCGGCCCCTCAAG ACCATCAAACGGCTGCCCAAGCTCAAGGCTGTGTTTGACTGTGTGGTGAACTCCCTGAAGAATGTCCTCA ACATCTTGATTGTCTACATGCTCTTCATGTTCATATTTGCCGTCATTGCGGTGCAGCTCTTCAAAGGGAA GTTTTTCTACTGCACAGATGAATCCAAGGAGCTGGAGAGGGACTGCAGGGGTCAGTATTTGGATTATGAG AAGGAGGAAGTGGAAGCTCAGCCCAGGCAGTGGAAGAAATACGACTTTCACTACGACAATGTGCTCTGGG CTCTGCTGACGCTGTTCACAGTGTCCACGGGAGAAGGCTGGCCCATGGTGCTGAAACACTCCGTGGATGC CACCTATGAGGAGCAGGGTCCAAGCCCTGGGTACCGCATGGAGCTGTCCATCTTCTACGTGGTCTACTTT GTGGTCTTTCCCTTCTTCTTCGTCAACATCTTTGTGGCTTTGATCATCATCACCTTCCAGGAGCAGGGGG ACAAGGTGATGTCTGAATGCAGCCTGGAGAAGAACGAGAGGGCTTGCATTGACTTCGCCATCAGCGCCAA ACCCCTGACACGGTACATGCCCCAAAACCGGCAGTCGTTCCAGTATAAGACGTGGACATTTGTGGTCTCC CCGCCCTTTGAATACTTCATCATGGCCATGATAGCCCTCAACACTGTGGTGCTGATGATGAAGTTCTATG ATGCACCCTATGAGTACGAGCTGATGCTGAAATGCCTGAACATCGTGTTCACATCCATGTTCTCCATGGA ATGCGTGCTGAAGATCATCGCCTTTGGGGTGCTGAACTATTTCAGAGATGCCTGGAATGTCTTTGACTTT GTCACTGTGTTGGGAAGTATTACTGATATTTTAGTAACAGAGATTGCGGAAACGAACAATTTCATCAACC TCAGCTTCCTCCGCCTCTTTCGAGCTGCGCGGCTGATCAAGCTGCTCCGCCAGGGCTACACCATCCGCAT CCTGCTGTGGACCTTTGTCCAGTCCTTCAAGGCCCTGCCCTACGTGTGTCTGCTCATTGCCATGCTGTTC TTCATCTACGCCATCATCGGCATGCAGGTGTTTGGGAATATTGCCCTGGATGATGACACCAGCATCAACC GCCACAACAACTTCCGGACGTTTTTGCAAGCCCTGATGCTGCTGTTCAGGAGCGCCACGGGGGAGGCCTG GCACGAGATCATGCTGTCCTGCCTGAGCAACCAGGCCTGTGATGAGCAGGCCAATGCCACCGAGTGTGGA AGTGACTTTGCCTACTTCTACTTCGTCTCCTTCATCTTCCTGTGCTCCTTTCTGATGTTGAACCTCTTTG TGGCTGTGATCATGGACAATTTTGAGTACCTCACGCGGGACTCTTCCATCCTAGGTCCTCACCACTTGGA TGAGTTCATCCGGGTCTGGGCTGAATACGACCCGGCTGCGTGTGGGCGCATCAGTTACAATGACATGTTT GAGATGCTGAAACACATGTCCCCGCCTCTGGGGCTGGGGAAGAAATGCCCTGCTCGAGTTGCTTACAAGC GCCTGGTTCGCATGAACATGCCCATCTCCAACGAGGACATGACTGTTCACTTCACGTCCACGCTGATGGC CCTCATCCGGACGGCACTGGAGATCAAGCTGGCCCCAGCTGGGACAAAGCAGCATCAGTGTGACGCGGAG TTGAGGAAGGAGATTTCCGTTGTGTGGGCCAATCTGCCCCAGAAGACTTTGGACTTGCTGGTACCACCCC ATAAGCCTGATGAGATGACAGTGGGGAAGGTTTATGCAGCTCTGATGATATTCGACTTCTACAAGCAGAA CAAAACCACCAGAGACCAGATGCAGCAGGCTCCTGGAGGCCTCTCCCAGATGGGTCCTGTGTCCCTGTTC CACCCTCTGAAGGCCACCCTGGAGCAGACACAGCCGGCTGTGCTCCGAGGAGCCCGGGTTTTCCTTCGAC AGAAGAGTTCCACCTCCCTCAGCAATGGCGGGGCCATACAAAACCAAGAGAGTGGCATCAAAGAGTCTGT CTCCTGGGGCACTCAAAGGACCCAGGATGCACCCCATGAGGCCAGGCCACCCCTGGAGCGTGGCCACTCC ACAGAGATCCCTGTGGGGCGGTCAGGAGCACTGGCTGTGGACGTTCAGATGCAGAGCATAACCCGGAGGG GCCCTGATGGGGAGCCCCAGCCTGGGCTGGAGAGCCAGGGTCGAGCGGCCTCCATGCCCCGCCTTGCGGC CGAGACTCAGCCCGTCACAGATGCCAGCCCCATGAAGCGCTCCATCTCCACGCTGGCCCAGCGGCCCCGT GGGACTCATCTTTGCAGCACCACCCCGGACCGCCCACCCCCTAGCCAGGCGTCGTCGCACCACCACCACC ACCGCTGCCACCGCCGCAGGGACAGGAAGCAGAGGTCCCTGGAGAAGGGGCCCAGCCTGTCTGCCGATAT GGATGGCGCACCAAGCAGTGCTGTGGGGCCGGGGCTGCCCCCGGGAGAGGGGCCTACAGGCTGCCGGCGG GAACGAGAGCGCCGGCAGGAGCGGGGCCGGTCCCAGGAGCGGAGGCAGCCCTCATCCTCCTCCTCGGAGA AGCAGCGCTTCTACTCCTGCGACCGCTTTGGGGGCCGTGAGCCCCCGAAGCCCAAGCCCTCCCTCAGCAG CCACCCAACGTCGCCAACAGCTGGCCAGGAGCCGGGACCCCACCCACAGGCCGGCTCAGCCGTGGGCTTT CCGAACACAACGCCCTGCTGCAGAGAGACCCCCTCAGCCAGCCCCTGGCCCCTGGCTCTCGAATTGGCTC TGACCCTTACCTGGGGCAGCGTCTGGACAGTGAGGCCTCTGTCCACGCCCTGCCTGAGGACACTCTCACT TTCGAGGAGGCTGTGGCCACCAACTCGGGCCGCTCCTCCAGGACTTCCTACGTGTCCTCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC235372 representing NM_001243812 Red=Cloning site Green=Tags(s) MVRFGDELGGRYGGPGGGERARGGGAGGAGGPGPGGLQPGQRVLYKQSIAQRARTMALYNPIPVKQNCFT VNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGI FCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQ VVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDAEPVGDFPCGKEAPARLCEGD TECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNL VLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDRNAEEKSPLDVLKRAA TKKSRNDLIHAEEGEDRFADLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRMVKAQSFYWVVLC VVALNTLCVAMVHYNQPRRLTTTLYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSVFE VVWAAIKPGSSFGISVLRALRLLRIFKVTKYWSSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQL FGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFGNYTL LNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAARQQNSAKARSVWEQ RASQLRLQNLRASCEALYSEMDPEERLRFATTRHLRPDMKTHLDRPLVVELGRDGARGPVGGKARPEAAE APEGVDPPRRHHRHRDKDKTPAAGDQDRAEAPKAESGEPGAREERPRPHRSHSKEAAGPPEARSERGRGP GPEGGRRHHRRGSPEEAAEREPRRHRAHRHQDPSKECAGAKGERRARHRGGPRAGPREAESGEEPARRHR ARHKAQPAHEAVEKETTEKEATEKEAEIVEADKEKELRNHQPREPHCDLETSGTVTVGPMHTLPSTCLQK VEEQPEDADNQRNVTRMGSQPPDPNTIVHIPVMLTGPLGEATVVPSGNVDLESQAEGKKEVEADDVMRSG PRPIVPYSSMFCLSPTNLLRRFCHYIVTMRYFEVVILVVIALSSIALAAEDPVRTDSPRNNALKYLDYIF TGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSGSKGKDINTIKSLRVLRVLRPLK TIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYE KEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGYRMELSIFYVVYF VVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNRQSFQYKTWTFVVS PPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDF VTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLF FIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNQACDEQANATECG SDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYNDMF EMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALEIKLAPAGTKQHQCDAE LRKEISVVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKTTRDQMQQAPGGLSQMGPVSLF HPLKATLEQTQPAVLRGARVFLRQKSSTSLSNGGAIQNQESGIKESVSWGTQRTQDAPHEARPPLERGHS TEIPVGRSGALAVDVQMQSITRRGPDGEPQPGLESQGRAASMPRLAAETQPVTDASPMKRSISTLAQRPR GTHLCSTTPDRPPPSQASSHHHHHRCHRRRDRKQRSLEKGPSLSADMDGAPSSAVGPGLPPGEGPTGCRR ERERRQERGRSQERRQPSSSSSEKQRFYSCDRFGGREPPKPKPSLSSHPTSPTAGQEPGPHPQAGSAVGF PNTTPCCRETPSASPWPLALELALTLTWGSVWTVRPLSTPCLRTLSLSRRLWPPTRAAPPGLPTCPP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001243812
ORF Size	6711 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001243812.2
RefSeq Size	9618 bp
RefSeq ORF	6714 bp
Locus ID	774
UniProt ID	Q00975
Protein Families	Druggable Genome, Ion Channels: Calcium, Transmembrane
Protein Pathways	Calcium signaling pathway, MAPK signaling pathway, Taste transduction, Type II diabetes mellitus
MW	252.2 kDa
Gene Summary	The protein encoded by this gene is the pore-forming subunit of an N-type voltage-dependent calcium channel, which controls neurotransmitter release from neurons. The encoded protein forms a complex with alpha-2, beta, and delta subunits to form the high-voltage activated channel. This channel is sensitive to omega-conotoxin-GVIA and omega-agatoxin-IIIA but insensitive to dihydropyridines. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

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