JARID2 (NM_001267040) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	JMJ
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC235154 representing NM_001267040 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGTATTTTGGAAGCTCTCAGGATGAGGAGGAAGTCGAGGAGGAAGATGATGAGACAGAAGACGTCA AAACAGCCACCAACAATGCTTCATCTTCATGCCAGTCGACCCCCAGGAAAGGAAAAACCCACAAACATGT TCACAACGGGCATGTTTTCAATGGTTCCAGCAGGTCAACACGGGAGAAGGAACCTGTTCAAAAACACAAA AGCAAAGAGGCCACTCCCGCAAAGGAGAAGCACAGCGATCACCGGGCTGACAGCCGCCGGGAGCAGGCTT CAGCTAACCACCCCGCAGCGGCCCCCTCCACGGGTTCCTCGGCCAAGGGGCTTGCTGCCACCCATCACCA CCCCCCTCTGCATCGGTCGGCTCAGGACTTACGGAAACAGGTTTCTAAGGTAAACGGAGTCACTCGAATG TCATCTCTGGGTGCAGGTGTAACCAGTGCCAAAAAGATGCGCGAGGTCAGACCTTCACCATCCAAAACTG TGAAGTACACTGCCACGGTGACGAAGGGGGCTGTCACATACACCAAAGCCAAGAGAGAACTGGTCAAGGA CACCAAACCCAATCACCACAAGCCCAGTTCCGCTGTCAACCACACAATCTCAGGGAAAACTGAAAGTAGC AATGCAAAAACCCGCAAACAGGTGCTATCCCTCGGGGGGGCGTCCAAGTCCACTGGGCCCGCCGTCAATG GCCTCAAGGTCAGTGGCAGGTTGAACCCAAAGTCATGCACTAAGGAGGTGGGGGGGCGGCAGCTGCGGGA GGGCCTGCAGCTGCGGGAGGGGCTGCGGAACTCCAAGAGGAGACTGGAAGAGGCACACCAGGCGGAGAAG CCGCAGTCGCCCCCCAAGAAGATGAAAGGGGCGGCTGGCCCCGCCGAAGGCCCTGGCAAGAAGGCCCCGG CCGAGAGAGGTCTGCTGAACGGACACGTGAAGAAGGAAGTGCCGGAGCGCAGTCTGGAGAGGAATCGGCC GAAGCGGGCCACGGCCGGGAAGAGCACGCCAGGCAGACAAGCACATGGCAAGGCGGACAGCGCCTCCTGT GAAAATCGTTCTACCTCGCAACCGGAGTCCGTGCACAAGCCGCAGGACTCGGGCAAGGCCGAGAAGGGCG GCGGCAAGGCCGGGTGGGCGGCCATGGACGAGATCCCCGTCCTCAGGCCCTCCGCCAAGGAGTTCCACGA TCCGCTCATCTACATCGAGTCGGTCCGCGCTCAGGTGGAGAAGTTCGGGATGTGCAGGGTGATCCCCCCT CCGGACTGGCGGCCCGAGTGCAAGCTCAACGATGAGATGCGGTTTGTCACGCAGATTCAGCACATCCACA AGCTGGGCCGGCGCTGGGGCCCCAACGTGCAGCGGCTGGCCTGCATCAAGAAGCACCTCAAATCTCAGGG CATCACCATGGACGAGCTCCCGCTCATAGGGGGCTGTGAGCTCGACCTGGCCTGCTTTTTCCGGCTGATT AATGAGATGGGCGGCATGCAGCAAGTGACTGACCTCAAAAAATGGAACAAACTAGCAGACATGCTGCGCA TCCCCAGAACTGCCCAGGACCGGCTGGCCAAGCTGCAGGAGGCCTACTGCCAGTACCTACTCTCCTACGA CTCCCTGTCCCCAGAGGAGCACCGGCGGCTGGAGAAGGAGGTGCTGATGGAGAAGGAGATCCTGGAGAAG CGCAAGGGGCCGCTGGAAGGCCACACAGAGAACGACCACCACAAGTTCCACCCTCTGCCCCGCTTCGAGC CCAAGAATGGGCTCATCCACGGCGTGGCCCCCAGGAACGGCTTCCGCAGCAAGCTCAAGGAGGTGGGCCA GGCCCAGTTGAAGACTGGCCGGCGGCGACTCTTCGCTCAGGAAAAAGAAGTGGTCAAGGAAGAGGAGGAG GACAAAGGCGTCCTCAATGACTTCCACAAGTGCATCTATAAGGGAAGGTCTGTTTCTCTAACAACTTTTT ATCGAACAGCGAGGAATATCATGAGCATGTGTTTCAGCAAGGAGCCTGCCCCAGCCGAAATCGAGCAAGA GTACTGGAGGCTAGTGGAAGAGAAGGACTGCCACGTGGCAGTGCACTGCGGCAAGGTGGACACCAACACT CACGGCAGTGGATTCCCAGTAGGAAAATCAGAACCCTTTTCGAGGCATGGATGGAACCTCACCGTCCTCC CCAATAACACAGGGTCCATCCTGCGTCACCTCGGTGCTGTGCCTGGAGTGACTATTCCCTGGCTAAATAT TGGCATGGTCTTTTCTACCTCATGCTGGTCTCGAGACCAAAATCACCTTCCATACATTGACTACTTACAC ACTGGTGCTGACTGCATTTGGTATTGCATTCCTGCTGAGGAGGAGAACAAGCTGGAAGATGTGGTCCACA CCCTGCTGCAAGCCAATGGCACCCCAGGGCTGCAGATGCTGGAAAGCAACGTCATGATCTCCCCGGAGGT GCTGTGCAAAGAGGGGATCAAGGTGCACAGGACCGTGCAGCAGAGTGGCCAGTTTGTCGTCTGCTTCCCG GGATCCTTTGTGTCCAAAGTGTGCTGTGGGTACAGCGTGTCTGAAACCGTGCACTTTGCTACCACCCAGT GGACAAGTATGGGCTTTGAGACCGCCAAGGAAATGAAGCGTCGCCATATAGCTAAGCCATTCTCCATGGA GAAGTTACTCTACCAGATTGCACAAGCAGAAGCAAAAAAAGAAAACGGTCCCACTCTCAGTACCATCTCA GCCCTCCTGGATGAGCTCAGGGATACAGAGCTGCGGCAGCGCAGGCAGCTGTTCGAGGCTGGCCTCCACT CCTCCGCACGCTATGGCAGCCACGATGGCAGCAGCACGGTGGCGGACGGGAAGAAAAAGCCTCGAAAGTG GCTGCAGTTGGAGACGTCAGAGAGGAGGTGTCAGATCTGCCAGCACCTGTGCTACCTGTCCATGGTGGTA CAAGAGAACGAAAACGTCGTGTTCTGTCTGGAGTGTGCTCTGCGCCACGTGGAGAAACAGAAGTCCTGCC GAGGGCTGAAGTTGATGTACCGCTACGATGAGGAACAGATTATCAGTCTGGTCAATCAGATCTGCGGCAA AGTGTCTGGTAAAAACGGCAGCATTGAGAACTGTCTCAGTAAACCCACACCAAAAAGAGGTCCCCGCAAG AGAGCGACAGTGGACGTGCCCCCCTCCCGTCTGTCAGCCTCCAGTTCATCCAAAAGTGCTTCGAGCTCAT CA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC235154 representing NM_001267040 Red=Cloning site Green=Tags(s) MVYFGSSQDEEEVEEEDDETEDVKTATNNASSSCQSTPRKGKTHKHVHNGHVFNGSSRSTREKEPVQKHK SKEATPAKEKHSDHRADSRREQASANHPAAAPSTGSSAKGLAATHHHPPLHRSAQDLRKQVSKVNGVTRM SSLGAGVTSAKKMREVRPSPSKTVKYTATVTKGAVTYTKAKRELVKDTKPNHHKPSSAVNHTISGKTESS NAKTRKQVLSLGGASKSTGPAVNGLKVSGRLNPKSCTKEVGGRQLREGLQLREGLRNSKRRLEEAHQAEK PQSPPKKMKGAAGPAEGPGKKAPAERGLLNGHVKKEVPERSLERNRPKRATAGKSTPGRQAHGKADSASC ENRSTSQPESVHKPQDSGKAEKGGGKAGWAAMDEIPVLRPSAKEFHDPLIYIESVRAQVEKFGMCRVIPP PDWRPECKLNDEMRFVTQIQHIHKLGRRWGPNVQRLACIKKHLKSQGITMDELPLIGGCELDLACFFRLI NEMGGMQQVTDLKKWNKLADMLRIPRTAQDRLAKLQEAYCQYLLSYDSLSPEEHRRLEKEVLMEKEILEK RKGPLEGHTENDHHKFHPLPRFEPKNGLIHGVAPRNGFRSKLKEVGQAQLKTGRRRLFAQEKEVVKEEEE DKGVLNDFHKCIYKGRSVSLTTFYRTARNIMSMCFSKEPAPAEIEQEYWRLVEEKDCHVAVHCGKVDTNT HGSGFPVGKSEPFSRHGWNLTVLPNNTGSILRHLGAVPGVTIPWLNIGMVFSTSCWSRDQNHLPYIDYLH TGADCIWYCIPAEEENKLEDVVHTLLQANGTPGLQMLESNVMISPEVLCKEGIKVHRTVQQSGQFVVCFP GSFVSKVCCGYSVSETVHFATTQWTSMGFETAKEMKRRHIAKPFSMEKLLYQIAQAEAKKENGPTLSTIS ALLDELRDTELRQRRQLFEAGLHSSARYGSHDGSSTVADGKKKPRKWLQLETSERRCQICQHLCYLSMVV QENENVVFCLECALRHVEKQKSCRGLKLMYRYDEEQIISLVNQICGKVSGKNGSIENCLSKPTPKRGPRK RATVDVPPSRLSASSSSKSASSSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001267040
ORF Size	3222 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001267040.1, NP_001253969.1
RefSeq Size	5612 bp
RefSeq ORF	3225 bp
Locus ID	3720
UniProt ID	Q92833
Protein Families	Transcription Factors
MW	120.1 kDa
Gene Summary	This gene encodes a Jumonji- and AT-rich interaction domain (ARID)-domain-containing protein. The encoded protein is a DNA-binding protein that functions as a transcriptional repressor. This protein interacts with the Polycomb repressive complex 2 (PRC2) which plays an essential role in regulating gene expression during embryonic development. This protein facilitates the recruitment of the PRC2 complex to target genes. Alternate splicing results in multiple transcript variants. Mutations in this gene are associated with chronic myeloid malignancies. [provided by RefSeq, May 2012]

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