ERCC6 (NM_001277059) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ARMD5; CKN2; COFS; COFS1; CSB; CSB-PGBD3; POF11; RAD26; UVSS1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC235146 representing NM_001277059 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAAATGAGGGAATCCCCCACTCAAGTCAAACTCAGGAGCAAGACTGTTTACAGAGTCAACCTGTCA GTAATAATGAAGAAATGGCAATCAAGCAAGAAAGTGGTGGTGATGGGGAGGTGGAGGAGTACCTCTCCTT TCGTTCTGTGGGTGACGGGCTGTCCACCTCTGCTGTGGGGTGCGCATCAGCAGCTCCGAGGAGAGGGCCA GCCCTGCTGCACATCGACCGACATCAGATCCAGGCAGTAGAGCCTAGCGCCCAGGCCCTTGAGCTGCAGG GTTTGGGTGTGGACGTCTATGACCAGGACGTGCTGGAACAGGGAGTGCTTCAGCAGGTGGACAATGCCAT CCATGAGGCCAGCCGTGCCTCCCAGCTCGTTGACGTGGAGAAGGAGTATCGGTCGGTCCTGGATGACCTC ACGTCATGTACGACATCCCTAAGGCAAATCAATAAAATTATTGAACAGCTTAGCCCTCAAGCTGCCACCA GCAGAGACATCAACAGGAAACTAGATTCTGTAAAACGACAGAAGTATAATAAGGAACAACAGCTAAAAAA GATCACTGCAAAACAAAAGCATCTCCAGGCCATCCTTGGAGGAGCAGAGGTGAAAATTGAACTAGATCAC GCCAGTCTGGAGGAGGATGCAGAGCCGGGGCCATCCAGTCTTGGCAGCATGCTCATGCCTGTCCAGGAGA CTGCCTGGGAAGAGCTCATCCGCACTGGCCAGATGACACCTTTTGGTACCCAGATCCCTCAGAAACAGGA GAAAAAGCCCAGAAAAATCATGCTTAATGAAGCATCAGGCTTCGAAAAGTATTTGGCAGATCAAGCAAAA CTGTCTTTTGAAAGGAAGAAGCAAGGTTGTAATAAAAGAGCAGCTAGAAAAGCTCCAGCCCCAGTCACGC CTCCAGCCCCAGTGCAAAATAAAAACAAACCAAACAAGAAAGCCAGAGTTCTGTCCAAAAAAGAGGAGCG TTTGAAAAAGCACATCAAGAAACTCCAGAAGAGGGCTTTGCAGTTCCAGGGGAAAGTGGGATTGCCAAAG GCAAGGAGACCTTGGGAGTCAGACATGAGGCCAGAGGCAGAGGGAGACTCTGAGGGTGAAGAGTCTGAGT ATTTCCCCACAGAGGAGGAGGAAGAGGAGGAAGATGACGAGGTGGAGGGGGCAGAGGCGGACCTGTCTGG AGATGGTACTGACTATGAGCTGAAGCCTCTGCCCAAGGGCGGGAAACGGCAGAAGAAAGTGCCAGTGCAG GAGATTGATGATGACTTTTTCCCAAGTTCTGGGGAAGAAGCTGAAGCTGCTTCTGTAGGAGAAGGAGGAG GAGGAGGTCGGAAAGTGGGAAGATACCGAGATGATGGAGATGAAGATTATTATAAGCAGCGGTTAAGTCC CAAGATGCCTCGAACACTAAGTTTACATGAAATAACTGACCTTTTAGAGACAGATGACAGCATAGAAGCA AGTGCTATAGTGATACAACCACCTGAAAATGCTACAGCACCTGTTTCTGATGAGGAATCAGGAGATGAAG AAGGTGGAACAATAAATAATCTGCCAGGTTCTTTGTTGCACACAGCTGCGTATCTTATTCAAGATGGCTC TGATGCTGAGTCTGACTCAGATGATCCCTCATACGCACCTAAAGATGACTCTCCTGATGAAGTTCCATCT ACGTTTACTGTGCAGCAACCTCCACCATCAAGGAGGAGGAAAATGACAAAAATTCTTTGCAAATGGAAAA AAGCCGACCTAACTGTACAACCCGTAGCAGGTAGAGTTACAGCACCACCAAACGATTTCTTCACCGTAAT GAGAACTCCCACAGAAATTCTTGAACTTTTTCTTGATGACGAGGTCATTGAACTCATTGTCAAGTACTCC AACTTATATGCTTGCAGTAAAGGTGTACATCTTGGCTTGACTAGCTCTGAATTCAAATGTTTTCTGGGAA TTATTTTTCTGAGTGGTTATGTCTCAGTTCCTAGAAGGCGTATGTTTTGGGAACAAAGAACAGATGTGCA TAATGTACTGGTTAGTGCTGCCATGAGACGTGACCGGTTTGAAACTATATTTTCTAATTTGCATGTTGCT GACAATGCAAATTTGGATCCAGTGGACAAATTTTCCAAATTGCGACCTCTCATAAGCAAACTTAATGAGA GATGCATGAAATTTGTTCCAAATGAAACATATTTCAGCTTTGATGAATTCATGGTTCCTTATTTTGGTCG TCACGGGTGCAAACAATTTATTCGGGGAAAGCCCATTCGGTTTGGCTATAAGTTTTGGTGTGGTGCCACC TGTCTGGGCTACATTTGCTGGTTTCAGCCGTATCAGGGTAAAAACCCAAATACTAAACATGAGGAATATG GTGTCGGTGCGTCACTTGTCCTTCAGTTTAGTGAGGCACTTACAGAGGCACACCCTGGACAATACCATTT TGTATTCAATAACTTTTTCACCAGTATTGCACTTCTTGATAAGCTCAGTTCAATGGGACATCAGGCAACA GGTACAGTGAGAAAGGATCACATTGACAGAGTTCCACTGGAATCAGATGTAGCTTTAAAGAAAAAAGAAA GAGGCACATTTGATTATCGAATTGATGGCAAAGGCAATATTGTCTGCAGATGGAATGATAACAGTGTTGT CACTGTTGCCTCATCTGGTGCTGGTATCCATCCCCTGTGTCTTGTCAGTCGTTACTCCCAGAAACTGAAA AAGAAGATACAAGTTCAGCAGCCAAACATGATCAAAGTGTATAACCAGTTCATGGGAGGCGTAGACAGAG CTGATGAAAACATTGATAAGTATCGGGCATCAATCCGTGGAAAGAAATGGTATTCAAGCCCTCTTTTGTT CTGTTTCGAACTGGTCTTACAAAATGCTTGGCAATTGCATAAAACATATGATGAGAAACCAGTGGATTTT CTGGAGTTTCGTCGACGTGTGGTATGCCATTATCTGGAGACCCATGGTCATCCTCCAGAACCTGGCCAAA AAGGAAGACCTCAGAAGCGTAACATTGACTCACGTTATGATGGCATAAATCATGTGATAGTCAAACAGGG AAAGCAAACGCGATGCGCTGAATGTCATAAGAACACAACTTTTCGATGTGAAAAATGTGATGTTGCCTTA CATGTGAAGTGTTCCGTTGAATATCACACTGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC235146 representing NM_001277059 Red=Cloning site Green=Tags(s) MPNEGIPHSSQTQEQDCLQSQPVSNNEEMAIKQESGGDGEVEEYLSFRSVGDGLSTSAVGCASAAPRRGP ALLHIDRHQIQAVEPSAQALELQGLGVDVYDQDVLEQGVLQQVDNAIHEASRASQLVDVEKEYRSVLDDL TSCTTSLRQINKIIEQLSPQAATSRDINRKLDSVKRQKYNKEQQLKKITAKQKHLQAILGGAEVKIELDH ASLEEDAEPGPSSLGSMLMPVQETAWEELIRTGQMTPFGTQIPQKQEKKPRKIMLNEASGFEKYLADQAK LSFERKKQGCNKRAARKAPAPVTPPAPVQNKNKPNKKARVLSKKEERLKKHIKKLQKRALQFQGKVGLPK ARRPWESDMRPEAEGDSEGEESEYFPTEEEEEEEDDEVEGAEADLSGDGTDYELKPLPKGGKRQKKVPVQ EIDDDFFPSSGEEAEAASVGEGGGGGRKVGRYRDDGDEDYYKQRLSPKMPRTLSLHEITDLLETDDSIEA SAIVIQPPENATAPVSDEESGDEEGGTINNLPGSLLHTAAYLIQDGSDAESDSDDPSYAPKDDSPDEVPS TFTVQQPPPSRRRKMTKILCKWKKADLTVQPVAGRVTAPPNDFFTVMRTPTEILELFLDDEVIELIVKYS NLYACSKGVHLGLTSSEFKCFLGIIFLSGYVSVPRRRMFWEQRTDVHNVLVSAAMRRDRFETIFSNLHVA DNANLDPVDKFSKLRPLISKLNERCMKFVPNETYFSFDEFMVPYFGRHGCKQFIRGKPIRFGYKFWCGAT CLGYICWFQPYQGKNPNTKHEEYGVGASLVLQFSEALTEAHPGQYHFVFNNFFTSIALLDKLSSMGHQAT GTVRKDHIDRVPLESDVALKKKERGTFDYRIDGKGNIVCRWNDNSVVTVASSGAGIHPLCLVSRYSQKLK KKIQVQQPNMIKVYNQFMGGVDRADENIDKYRASIRGKKWYSSPLLFCFELVLQNAWQLHKTYDEKPVDF LEFRRRVVCHYLETHGHPPEPGQKGRPQKRNIDSRYDGINHVIVKQGKQTRCAECHKNTTFRCEKCDVAL HVKCSVEYHTE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001277059
ORF Size	3183 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001277059.2
RefSeq Size	3535 bp
RefSeq ORF	3186 bp
Locus ID	2074
UniProt ID	P0DP91
Protein Families	Druggable Genome
Protein Pathways	Nucleotide excision repair
MW	119.9 kDa
Gene Summary	This gene encodes a DNA-binding protein that is important in transcription-coupled excision repair. The encoded protein has ATP-stimulated ATPase activity, interacts with several transcription and excision repair proteins, and may promote complex formation at DNA repair sites. Mutations in this gene are associated with Cockayne syndrome type B and cerebrooculofacioskeletal syndrome 1. Alternative splicing occurs between a splice site from exon 5 of this gene to the 3' splice site upstream of the open reading frame (ORF) of the adjacent gene, piggyback-derived-3 (GeneID:267004), which activates the alternative polyadenylation site downstream of the piggyback-derived-3 ORF. The resulting transcripts encode a fusion protein that shares sequence with the product of each individual gene. [provided by RefSeq, Mar 2016]

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