CSDE1 (NM_001242891) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	D1S155E; UNR
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234955 representing NM_001242891 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAACGTTTTTACTGTGTCATCAGATCCTCATCCCTCTCCTGCAGCCCCTCCTTCACTTTCTTTAC CCTTATCTTCATCTTCTACCTCATCTGGGACTAAAAAACAAAAGAGGACCCCAACTTATCAGAGATCTAT GAGCTTTGATCCAAACCTTCTCCACAACAATGGACATAATGGGTACCCTAATGGTACTTCAGCAGCACTG CGTGAAACTGGGGTTATTGAAAAACTGTTAACCTCTTACGGATTTATTCAGTGTTCAGAACGTCAAGCTA GACTTTTCTTCCACTGTTCACAGTATAATGGCAACCTGCAAGACTTAAAAGTAGGAGATGATGTTGAATT TGAAGTATCATCGGACCGACGGACTGGGAAACCCATTGCTGTTAAACTGGTGAAGATAAAACAAGAAATC CTCCCTGAAGAACGAATGAATGGACAAGTTGTGTGCGCTGTTCCTCACAACTTAGAGAGTAAATCTCCAG CTGCCCCGGGTCAGAGTCCAACAGGGAGTGTATGCTACGAACGTAATGGGGAAGTGTTTTATCTGACTTA CACCCCTGAAGATGTCGAAGGGAACGTTCAGCTGGAAACTGGAGATAAAATAAACTTTGTAATTGATAAC AATAAACATACTGGTGCTGTAAGTGCTCGCAACATTATGCTGTTGAAAAAGAAACAAGCCCGCTGTCAGG GAGTAGTTTGTGCCATGAAGGAGGCATTTGGCTTTATTGAAAGAGGTGATGTTGTAAAAGAGATATTCTT TCACTATAGTGAATTTAAGGGTGACTTAGAAACCTTACAGCCTGGCGATGATGTGGAATTCACAATCAAG GACAGAAATGGTAAAGAAGTTGCAACAGATGTCAGACTATTGCCTCAAGGAACAGTCATTTTTGAAGATA TCAGCATTGAACATTTTGAAGGAACTGTAACCAAAGTTATCCCAAAAGTACCCAGTAAAAACCAGAATGA CCCATTGCCAGGACGCATCAAAGTTGACTTTGTGATCCCTAAAGAACTTCCCTTTGGAGACAAAGATACG AAATCCAAGGTGACCCTGCTGGAAGGTGACCATGTTAGGTTTAATATTTCAACAGACCGACGTGACAAAT TAGAGCGAGCAACCAATATAGAAGTTCTGTCAAATACATTTCAGTTCACTAATGAAGCCCGAGAAATGGG TGTGATTGCTGCCATGAGAGATGGTTTTGGTTTCATCAAGTGTGTGGATCGTGATGTTCGTATGTTCTTC CACTTCAGTGAAATTCTGGATGGGAACCAGCTCCATATTGCAGATGAAGTAGAGTTTACTGTGGTTCCTG ATATGCTCTCTGCTCAAAGAAATCATGCTATTAGGATTAAAAAACTTCCCAAGGGCACGGTTTCATTTCA TTCCCATTCAGATCACCGTTTTCTGGGCACGGTAGAAAAAGAAGCCACTTTTTCCAATCCTAAAACCACT AGCCCAAATAAAGGCAAAGAGAAGGAGGCTGAGGATGGCATTATTGCTTATGATGACTGTGGGGTGAAAC TGACTATTGCTTTTCAAGCCAAGGATGTGGAAGGATCTACTTCTCCTCAAATAGGAGATAAGGTTGAATT TAGTATTAGTGACAAACAGAGGCCTGGACAGCAGGTTGCAACTTGTGTGCGACTTTTAGGTCGTAATTCT AACTCCAAGAGGCTCTTGGGTTATGTGGCAACTCTGAAGGATAATTTTGGATTTATTGAAACAGCCAATC ATGATAAGGAAATCTTTTTCCATTACAGTGAGTTCTCTGGTGATGTTGATAGCCTGGAACTGGGGGACAT GGTCGAGTATAGCTTGTCCAAAGGCAAAGGCAACAAAGTCAGTGCAGAAAAAGTGAACAAAACACACTCA GTGAATGGCATTACTGAGGAAGCTGATCCCACCATTTACTCTGGCAAAGTAATTCGCCCCCTGAGGAGTG TTGATCCAACACAGACTGAGTACCAAGGAATGATTGAGATTGTGGAGGAGGGCGATATGAAAGGTGAGGT CTATCCATTTGGCATCGTTGGGATGGCCAACAAAGGGGATTGCCTGCAGAAAGGGGAGAGCGTCAAGTTC CAATTGTGTGTCCTGGGCCAAAATGCACAAACTATGGCTTACAACATCACACCCCTGCGCAGGGCCACAG TGGAATGTGTGAAAGATCAGTTTGGCTTCATTAACTATGAAGTAGGAGATAGCAAGAAGCTCTTTTTCCA TGTGAAAGAAGTTCAGGATGGCATTGAGCTACAGGCAGGAGATGAGGTGGAGTTCTCAGTGATTCTTAAT CAGCGCACTGGCAAGTGCAGCGCCTGTAATGTTTGGCGAGTCTGTGAGGGCCCCAAGGCTGTTGCAGCTC CTCGACCTGATCGGTTGGTCAATCGCTTGAAGAATATCACTCTGGATGATGCCAGTGCTCCTCGCCTAAT GGTTCTTCGTCAGCCAAGGGGACCAGATAACTCAATGGGGTTTGGTGCAGAAAGAAAGATCCGTCAAGCT GGTGTCATTGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234955 representing NM_001242891 Red=Cloning site Green=Tags(s) MENVFTVSSDPHPSPAAPPSLSLPLSSSSTSSGTKKQKRTPTYQRSMSFDPNLLHNNGHNGYPNGTSAAL RETGVIEKLLTSYGFIQCSERQARLFFHCSQYNGNLQDLKVGDDVEFEVSSDRRTGKPIAVKLVKIKQEI LPEERMNGQVVCAVPHNLESKSPAAPGQSPTGSVCYERNGEVFYLTYTPEDVEGNVQLETGDKINFVIDN NKHTGAVSARNIMLLKKKQARCQGVVCAMKEAFGFIERGDVVKEIFFHYSEFKGDLETLQPGDDVEFTIK DRNGKEVATDVRLLPQGTVIFEDISIEHFEGTVTKVIPKVPSKNQNDPLPGRIKVDFVIPKELPFGDKDT KSKVTLLEGDHVRFNISTDRRDKLERATNIEVLSNTFQFTNEAREMGVIAAMRDGFGFIKCVDRDVRMFF HFSEILDGNQLHIADEVEFTVVPDMLSAQRNHAIRIKKLPKGTVSFHSHSDHRFLGTVEKEATFSNPKTT SPNKGKEKEAEDGIIAYDDCGVKLTIAFQAKDVEGSTSPQIGDKVEFSISDKQRPGQQVATCVRLLGRNS NSKRLLGYVATLKDNFGFIETANHDKEIFFHYSEFSGDVDSLELGDMVEYSLSKGKGNKVSAEKVNKTHS VNGITEEADPTIYSGKVIRPLRSVDPTQTEYQGMIEIVEEGDMKGEVYPFGIVGMANKGDCLQKGESVKF QLCVLGQNAQTMAYNITPLRRATVECVKDQFGFINYEVGDSKKLFFHVKEVQDGIELQAGDEVEFSVILN QRTGKCSACNVWRVCEGPKAVAAPRPDRLVNRLKNITLDDASAPRLMVLRQPRGPDNSMGFGAERKIRQA GVID TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001242891
ORF Size	2532 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001242891.1, NP_001229820.1
RefSeq Size	4330 bp
RefSeq ORF	2535 bp
Locus ID	7812
UniProt ID	O75534
Protein Families	Transcription Factors
MW	94.2 kDa
Gene Summary	RNA-binding protein. Required for internal initiation of translation of human rhinovirus RNA. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain.[UniProtKB/Swiss-Prot Function]

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