KIF22 (NM_001256270) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	A-328A3.2; KID; KNSL4; OBP; OBP-1; OBP-2; SEMDJL2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234521 representing NM_001256270 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACAGCTGCTCTCTAGAGATTGCTAACTGGAGGAACCACCAGGAGACTCTCAAATACCAGTTTGATG CCTTCTATGGGGAGAGGAGTACTCAGCAGGACATCTATGCAGGTTCAGTGCAGCCCATCCTAAGGCACTT GCTGGAAGGGCAGAATGCCAGTGTGCTTGCCTATGGACCCACAGGAGCTGGGAAGACGCACACAATGCTG GGCAGCCCAGAGCAACCTGGGGTGATCCCGCGGGCTCTCATGGACCTCCTGCAGCTCACAAGGGAGGAGG GTGCCGAGGGCCGGCCATGGGCCCTTTCTGTCACCATGTCTTACCTAGAGATCTACCAGGAGAAGGTATT AGACCTCCTGGACCCTGCTTCGGGAGACCTGGTAATCCGAGAAGACTGCCGGGGGAATATCCTGATTCCG GGTCTCTCCCAGAAGCCCATCAGTAGCTTTGCTGATTTTGAGCGGCACTTCCTGCCAGCCAGTCGAAATC GGACTGTAGGAGCCACCCGGCTCAACCAGCGCTCCTCCCGCAGTCATGCTGTGCTCCTGGTCAAGGTGGA CCAGCGGGAACGTTTGGCCCCATTTCGCCAGCGAGAGGGAAAACTCTACCTGATTGACTTGGCTGGGTCA GAGGACAACCGGCGCACAGGCAACAAGGGCCTTCGGCTAAAAGAGAGTGGAGCCATCAACACCTCCCTGT TTGTCCTGGGCAAAGTGGTAGATGCGCTGAATCAGGGCCTCCCTCGTGTACCTTATCGGGACAGCAAGCT CACTCGCCTATTGCAGGACTCTCTGGGTGGCTCAGCCCACAGTATCCTTATTGCCAACATTGCCCCTGAG AGACGCTTCTACCTAGACACAGTCTCCGCACTCAACTTTGCTGCCAGGTCCAAGGAGGTGATCAATCGGC CTTTTACCAATGAGAGCCTGCAGCCTCATGCCTTGGGACCTGTTAAGCTGTCTCAGAAAGAATTGCTTGG TCCACCAGAGGCAAAGAGAGCCCGAGGCCCTGAGGAAGAGGAGATCGGGAGCCCTGAGCCCATGGCAGCT CCAGCCTCTGCCTCCCAGAAACTCAGCCCCCTACAGAAGCTAAGCAGCATGGACCCGGCCATGCTGGAGC GCCTCCTCAGCTTGGACCGTCTGCTTGCCTCCCAGGGGAGCCAGGGGGCCCCTCTGTTGAGTACCCCAAA GCGAGAGCGGATGGTGCTAATGAAGACAGTGGAAGAGAAGGACCTAGAGATTGAGAGGCTTAAGACGAAG CAAAAAGAACTGGAGGCCAAGATGTTGGCCCAGAAGGCTGAGGAAAAGGAGAACCATTGTCCCACAATGC TCCGGCCCCTTTCACATCGCACAGTCACAGGGGCAAAGCCCCTGAAAAAGGCTGTGGTGATGCCCCTACA GCTAATTCAGGAGCAGGCAGCATCCCCAAATGCCGAGATCCACATCCTGAAGAATAAAGGCCGGAAGAGA AAGCTGGAGTCCCTGGATGCCCTAGAGCCTGAGGAGAAGGCTGAGGACTGCTGGGAGCTACAGATCAGCC CGGAGCTACTGGCTCATGGGCGCCAAAAAATACTGGATCTGCTGAACGAAGGCTCAGCCCGAGATCTCCG CAGTCTTCAGCGCATTGGCCCGAAGAAGGCCCAGCTAATCGTGGGCTGGCGGGAGCTCCACGGCCCCTTC AGCCAGGTGGAGGACCTGGAACGCGTGGAGGGCATAACGGGGAAACAGATGGAGTCCTTCCTGAAGGCAA ACATCCTGGGTCTCGCCGCCGGCCAGCGCTGTGGCGCCTCC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234521 representing NM_001256270 Red=Cloning site Green=Tags(s) MDSCSLEIANWRNHQETLKYQFDAFYGERSTQQDIYAGSVQPILRHLLEGQNASVLAYGPTGAGKTHTML GSPEQPGVIPRALMDLLQLTREEGAEGRPWALSVTMSYLEIYQEKVLDLLDPASGDLVIREDCRGNILIP GLSQKPISSFADFERHFLPASRNRTVGATRLNQRSSRSHAVLLVKVDQRERLAPFRQREGKLYLIDLAGS EDNRRTGNKGLRLKESGAINTSLFVLGKVVDALNQGLPRVPYRDSKLTRLLQDSLGGSAHSILIANIAPE RRFYLDTVSALNFAARSKEVINRPFTNESLQPHALGPVKLSQKELLGPPEAKRARGPEEEEIGSPEPMAA PASASQKLSPLQKLSSMDPAMLERLLSLDRLLASQGSQGAPLLSTPKRERMVLMKTVEEKDLEIERLKTK QKELEAKMLAQKAEEKENHCPTMLRPLSHRTVTGAKPLKKAVVMPLQLIQEQAASPNAEIHILKNKGRKR KLESLDALEPEEKAEDCWELQISPELLAHGRQKILDLLNEGSARDLRSLQRIGPKKAQLIVGWRELHGPF SQVEDLERVEGITGKQMESFLKANILGLAAGQRCGAS SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001256270
ORF Size	1791 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001256270.1, NP_001243199.1
RefSeq Size	2296 bp
RefSeq ORF	1794 bp
Locus ID	3835
UniProt ID	Q14807
Protein Families	Druggable Genome
MW	66.8 kDa
Gene Summary	The protein encoded by this gene is a member of the kinesin-like protein family. The family members are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. The C-terminal half of this protein has been shown to bind DNA. Studies with the Xenopus homolog suggests its essential role in metaphase chromosome alignment and maintenance. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

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