NPRL3 (NM_001243247) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	C16orf35; CGTHBA; FFEVF3; HS-40; MARE; NPR3; RMD11
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234270 representing NM_001243247 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGTGGCCAAAAATTTGAACTGAAGATTGATAATGTGCGATTTGTTGGGCACCCAACACTGCTACAGC ATGCTCTGGGGCAGATCTCCAAAACAGATCCTTCCCCGAAGAGGGAAGCACCTACTATGATTCTTTTTAA TGTGGTGTTTGCACTGAGGGCCAACGCAGACCCGTCAGTGATAAACTGTCTGCATAACCTGTCCCGTCGT ATCGCCACCGTGCTGCAGCACGAGGAGCGCCGCTGCCAGTACCTCACCCGGGAGGCCAAGCTGATCCTGG CGCTCCAGGATGAGGTGTCCGCCATGGCTGATGGAAATGAAGGTCCTCAGTCCCCATTCCATCACATCCT GCCCAAGTGCAAGCTGGCCAGGGACCTCAAGGAAGCTTATGACAGCCTGTGCACGTCGGGCGTAGTTCGG CTTCACATCAACAGCTGGCTGGAGGTGAGCTTCTGCCTGCCCCACAAGATCCACTATGCGGCCTCCAGTC TGATCCCCCCAGAGGCCATCGAACGGAGCCTGAAAGCCATCCGCCCCTACCATGCCCTGCTGCTGCTCAG TGATGAGAAGTCCTTGCTGGGTGAGCTTCCTATTGACTGCTCCCCTGCCCTAGTGCGGGTGATCAAGACC ACATCTGCTGTGAAGAACCTGCAGCAGCTAGCCCAAGATGCGGACCTGGCCTTGCTGCAGGTTTTCCAGC TTGCAGCTCATCTGGTGTACTGGGGCAAGGCCATCATCATCTACCCGCTGTGTGAGAACAACGTCTACAT GCTGTCTCCCAATGCCAGCGTATGTCTGTACTCCCCGCTGGCCGAGCAGTTCTCCCACCAGTTCCCATCT CATGACCTGCCGTCCGTTCTTGCCAAGTTCTCCTTGCCGGTCTCCTTGTCAGAATTTAGGAATCCCCTGG CCCCCGCTGTGCAGGAGACCCAGCTCATCCAGATGGTGGTGTGGATGCTGCAGCGCCGGCTTCTCATCCA GCTGCACACCTATGTCTGCCTGATGGCCTCACCCAGCGAGGAGGAGCCCCGTCCGCGAGAGGACGACGTC CCCTTCACTGCCCGGGTCGGCGGTCGCAGCCTCAGCACGCCCAACGCCCTCAGCTTTGGCTCCCCAACCA GCAGCGATGACATGACCCTCACCAGCCCCAGCATGGACAACTCCAGCGCAGAGCTACTTCCCAGCGGGGA CTCGCCACTGAACCAGAGGATGACGGAGAACCTGCTGGCCAGCCTGTCGGAGCATGAACGCGCAGCCATC CTCAGTGTACCCGCAGCCCAGAACCCTGAGGACCTCCGCATGTTTGCCAGGCTCCTTCACTACTTCCGCG GCCGCCACCACCTGGAGGAGATTATGTACAACGAGAACACGCGGCGCTCCCAGCTGCTCATGCTGTTTGA CAAGTTCCGCAGCGTGCTGGTGGTGACCACCCACGAGGACCCTGTCATTGCCGTCTTCCAGGCTCTGCTC CCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234270 representing NM_001243247 Red=Cloning site Green=Tags(s) MCGQKFELKIDNVRFVGHPTLLQHALGQISKTDPSPKREAPTMILFNVVFALRANADPSVINCLHNLSRR IATVLQHEERRCQYLTREAKLILALQDEVSAMADGNEGPQSPFHHILPKCKLARDLKEAYDSLCTSGVVR LHINSWLEVSFCLPHKIHYAASSLIPPEAIERSLKAIRPYHALLLLSDEKSLLGELPIDCSPALVRVIKT TSAVKNLQQLAQDADLALLQVFQLAAHLVYWGKAIIIYPLCENNVYMLSPNASVCLYSPLAEQFSHQFPS HDLPSVLAKFSLPVSLSEFRNPLAPAVQETQLIQMVVWMLQRRLLIQLHTYVCLMASPSEEEPRPREDDV PFTARVGGRSLSTPNALSFGSPTSSDDMTLTSPSMDNSSAELLPSGDSPLNQRMTENLLASLSEHERAAI LSVPAAQNPEDLRMFARLLHYFRGRHHLEEIMYNENTRRSQLLMLFDKFRSVLVVTTHEDPVIAVFQALL P TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001243247
ORF Size	1473 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001243247.1, NP_001230176.1
RefSeq Size	2875 bp
RefSeq ORF	1476 bp
Locus ID	8131
MW	55.5 kDa
Gene Summary	The function of the encoded protein is not known. [provided by RefSeq, Aug 2011]

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