NDRG1 (NM_001258433) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CAP43; CMT4D; DRG-1; DRG1; GC4; HMSNL; NDR1; NMSL; PROXY1; RIT42; RTP; TARG1; TDD5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC233730 representing NM_001258433 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGGAGATCACCCAGCACTTTGCCGTCTGCCACGTGGACGCCCCTGGCCAGCAGGACGGCGCAGCCT CCTTCCCCGCAGGGTACATGTACCCCTCCATGGATCAGCTGGCTGAAATGCTTCCTGGAGTCCTTCAACA GTTTGGGCTGAAAAGCATTATTGGCATGGGAACAGGAGCAGGCGCCTACATCCTAACTCGATTTGCTCTA AACAACCCTGAGATGGTGGAGGGCCTTGTCCTTATCAACGTGAACCCTTGTGCGGAAGGCTGGATGGACT GGGCCGCCTCCAAGATCTCAGGATGGACCCAAGCTCTGCCGGACATGGTGGTGTCCCACCTTTTTGGGAA GGAAGAAATGCAGAGTAACGTGGAAGTGGTCCACACCTACCGCCAGCACATTGTGAATGACATGAACCCC GGCAACCTGCACCTGTTCATCAATGCCTACAACAGCCGGCGCGACCTGGAGATTGAGCGACCAATGCCGG GAACCCACACAGTCACCCTGCAGTGCCCTGCTCTGTTGGTGGTTGGGGACAGCTCGCCTGCAGTGGATGC CGTGGTGGAGTGCAACTCAAAATTGGACCCAACAAAGACCACTCTCCTCAAGATGGCGGACTGTGGCGGC CTCCCGCAGATCTCCCAGCCGGCCAAGCTCGCTGAGGCCTTCAAGTACTTCGTGCAGGGCATGGGATACA TGCCCTCGGCTAGCATGACCCGCCTGATGCGGTCCCGCACAGCCTCTGGTTCCAGCGTCACTTCTCTGGA TGGCACCCGCAGCCGCTCCCACACCAGCGAGGGCACCCGAAGCCGCTCCCACACCAGCGAGGGCACCCGC AGCCGCTCGCACACCAGCGAGGGGGCCCACCTGGACATCACCCCCAACTCGGGTGCTGCTGGGAACAGCG CCGGGCCCAAGTCCATGGAGGTCTCCTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC233730 representing NM_001258433 Red=Cloning site Green=Tags(s) MQEITQHFAVCHVDAPGQQDGAASFPAGYMYPSMDQLAEMLPGVLQQFGLKSIIGMGTGAGAYILTRFAL NNPEMVEGLVLINVNPCAEGWMDWAASKISGWTQALPDMVVSHLFGKEEMQSNVEVVHTYRQHIVNDMNP GNLHLFINAYNSRRDLEIERPMPGTHTVTLQCPALLVVGDSSPAVDAVVECNSKLDPTKTTLLKMADCGG LPQISQPAKLAEAFKYFVQGMGYMPSASMTRLMRSRTASGSSVTSLDGTRSRSHTSEGTRSRSHTSEGTR SRSHTSEGAHLDITPNSGAAGNSAGPKSMEVSC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001258433
ORF Size	939 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001258433.2
RefSeq Size	3017 bp
RefSeq ORF	942 bp
Locus ID	10397
UniProt ID	Q92597
MW	34.1 kDa
Gene Summary	This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]

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