TFPI2 (NM_001271003) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	PP5; REF1; TFPI-2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC232196 representing NM_001271003 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCCCGCTCGCCCCCTGGGGCTGTCGATTCTGCTGCTTTTCCTGACGGAGGCTGCACTGGGCGATG CTGCTCAGGAGCCAACAGACTACGGACCCTGCCGGGCCCTACTTCTCCGTTACTACTACGACAGGTACAC GCAGAGCTGCCGCCAGTTCCTGTACGGGGGCTGCGAGGGCAACGCCAACAATTTCTACACCTGGGAGGCT TGCGACGATGCTTGCTGGAGGATAGAAAAAGTTCCCAAAGTTTGCCGGCTGCAAGTGAGTGTGGACGACC AGTGTGAGGGGTCCACAGAAAAGTATTTCTTTAATCTAAGTTCCATGACATGTGAAAAATTCTTTTCCGG TGGGTGTCACCGGAACCGGATTGAGAACAGGTTTCCAGATGAAGCTACTTGTATGGGCTTCTGCGCACCA AAGAAAATTCCATCATTTTGCTACAGTCCAAAAGATGAGGGACTGTGCTCTGCCAATGTGACTCGCTATT ATTTTAATCCAAGATACAGAACCTGTGATGCTTTCACCTATACTGGCTGTGGAGGGAATGACAATAACTT TGTTAGCAGGGAGGATTGCAAACGTGCATGTGCAAAAGCTTTGAAAAAGAAAAAGAAGATGCCAAAGCTT CGCTTTGCCAGTAGAATCCGGAAAATTCGGAAGAAGCAATTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC232196 representing NM_001271003 Red=Cloning site Green=Tags(s) MDPARPLGLSILLLFLTEAALGDAAQEPTDYGPCRALLLRYYYDRYTQSCRQFLYGGCEGNANNFYTWEA CDDACWRIEKVPKVCRLQVSVDDQCEGSTEKYFFNLSSMTCEKFFSGGCHRNRIENRFPDEATCMGFCAP KKIPSFCYSPKDEGLCSANVTRYYFNPRYRTCDAFTYTGCGGNDNNFVSREDCKRACAKALKKKKKMPKL RFASRIRKIRKKQF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271003
ORF Size	672 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271003.2
RefSeq Size	2411 bp
RefSeq ORF	675 bp
Locus ID	7980
UniProt ID	P48307
Protein Families	Secreted Protein
MW	26.2 kDa
Gene Summary	This gene encodes a member of the Kunitz-type serine proteinase inhibitor family. The protein can inhibit a variety of serine proteases including factor VIIa/tissue factor, factor Xa, plasmin, trypsin, chymotryspin and plasma kallikrein. This gene has been identified as a tumor suppressor gene in several types of cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

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